| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Dongmei Wang, Junqiang Yan, Jing Chen, Wenlan Wu, Xiaoying Zhu, Yong Wan. Naringin Improves Neuronal Insulin Signaling, Brain Mitochondrial Function, and Cognitive Function in High-Fat Diet-Induced Obese Mice. Cellular and molecular neurobiology. vol 35. issue 7. 2016-06-24. PMID:25939427. |
then the protein levels of insulin signaling pathway and the amp-activated protein kinase (ampk) in the hippocampus were detected by western blot method. |
2016-06-24 |
2023-08-13 |
mouse |
| Haiyan Zhang, Yihang Li, Jie Hu, Wen-Jun Shen, Madhurima Singh, Xiaoming Hou, Alex Bittner, Stefanie Bittner, Yuan Cortez, Juveria Tabassum, Fredric B Kraemer, Salman Azha. Effect of Creosote Bush-Derived NDGA on Expression of Genes Involved in Lipid Metabolism in Liver of High-Fructose Fed Rats: Relevance to NDGA Amelioration of Hypertriglyceridemia and Hepatic Steatosis. PloS one. vol 10. issue 9. 2016-05-31. PMID:26394137. |
western blot analysis indicated that ndga administration upregulated hepatic insulin signaling (p-akt), ampk activity (p-ampk), mlycd, and pparα protein levels, but decreased scd1, acc1 and acc2 protein content and also inactivated acc1 activity (increased p-acc1). |
2016-05-31 |
2023-08-13 |
rat |
| Tomokazu Tanaka, Yoshihiko Kitajima, Shuusuke Miyake, Kazuyoshi Yanagihara, Hiromitsu Hara, Aki Nishijima-Matsunobu, Koichi Baba, Masaaki Shida, Kota Wakiyama, Jun Nakamura, Hirokazu Noshir. The Apoptotic Effect of HIF-1α Inhibition Combined with Glucose plus Insulin Treatment on Gastric Cancer under Hypoxic Conditions. PloS one. vol 10. issue 9. 2016-05-17. PMID:26339797. |
a western blot analysis showed that the membranous expression of glut1 in kd cells was elevated by glucose and/or insulin treatments, indicating that the gi-induced glucose uptake is mediated by the increased translocation of glut1 on the cell membrane. |
2016-05-17 |
2023-08-13 |
Not clear |
| Yi-Min Chen, Jin-Fang Zhao, Yong-Lin Liu, Jie Chen, Rong-Lin Jian. Chronic ethanol treatment of human hepatocytes inhibits the activation of the insulin signaling pathway by increasing cytosolic free calcium levels. International journal of molecular medicine. vol 36. issue 3. 2016-05-09. PMID:26165524. |
furthermore, insulin was added to the ethanol‑treated l‑02 cells, and the phosphorylation levels of pi3k and protein kinase b (pkb) were determined by western blot analysis before and after ca(2+) blockage. |
2016-05-09 |
2023-08-13 |
human |
| Vincenzo Cardinale, Rosa Puca, Guido Carpino, Gaia Scafetta, Anastasia Renzi, Michele De Canio, Francesca Sicilia, Lorenzo Nevi, Domenico Casa, Rocco Panetta, Pasquale Bartolomeo Berloco, Lola M Reid, Giorgio Federici, Eugenio Gaudio, Marella Maroder, Domenico Alvar. Adult Human Biliary Tree Stem Cells Differentiate to β-Pancreatic Islet Cells by Treatment with a Recombinant Human Pdx1 Peptide. PloS one. vol 10. issue 8. 2016-05-09. PMID:26252949. |
differentiation toward pancreatic islet cells were evaluated by the expression of the β-cell transcription factors, pdx1 and musculoapo-neurotic fibrosarcoma oncogene homolog a, and of the pancreatic hormones, insulin, glucagon, and somatostatin, investigated by real time polymerase chain reaction, western blot, light microscopy and immunofluorescence. |
2016-05-09 |
2023-08-13 |
human |
| M R King, N J Anderson, C Liu, E Law, M Cundiff, T M Mixcoatl-Zecuatl, C G Jolival. Activation of the insulin-signaling pathway in sciatic nerve and hippocampus of type 1 diabetic rats. Neuroscience. vol 303. 2016-05-06. PMID:26149351. |
fresh sciatic nerve and hippocampus from control and diabetic rats were incubated with varied ex vivo concentrations of insulin and phosphorylation levels of insulin receptor and glycogen synthase kinase-3 (gsk3β) were assessed by western blot analysis. |
2016-05-06 |
2023-08-13 |
rat |
| Fredrick J Rosario, Theresa L Powell, Thomas Jansso. Activation of placental insulin and mTOR signaling in a mouse model of maternal obesity associated with fetal overgrowth. American journal of physiology. Regulatory, integrative and comparative physiology. vol 310. issue 1. 2016-05-02. PMID:26491103. |
using western blot analysis, placental mtor activity was determined along with energy, inflammatory, leptin, and insulin signaling pathways (upstream modulators of mtor). |
2016-05-02 |
2023-08-13 |
mouse |
| Jiang-Kui Liu, Yi-Hui Shen, Xiang-Chuan Liu, Hui Zhang, Hui Wu, Ming Yi. [Effects of telmisartan on nonalcoholic steatohepatitis rat model by activating peroxisome proliferator-activated receptor r]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology. vol 19. issue 8. 2016-04-23. PMID:22152320. |
serum alanine aminotransferase(alt), aspartate aminotransferase(ast), fasting blood glucose(fbg), fasting insulin(fins), homa-ir(homeostasis model assessment insulin resistance), serum tnf-a and adiponectin were detected and analyzed.western blot and rt-pcr were used to detect pparr expression in hepatic tissues on protein and mrna levels. |
2016-04-23 |
2023-08-12 |
rat |
| Xiaojing Hu, Liyi Chi, Wentao Zhang, Tiao Bai, Wei Zhao, Zhanbin Feng, Hongyan Tia. Down-regulation of the miR-543 alleviates insulin resistance through targeting the SIRT1. Biochemical and biophysical research communications. vol 468. issue 4. 2016-04-19. PMID:26562529. |
in addition, the overexpression of the mir-543 by the lentivirus-mediated gene transfer markedly impaired the insulin signaling assessed by the western blot analysis of the glycogen synthesis and the phosphorylation of akt and gsk3β. |
2016-04-19 |
2023-08-13 |
human |
| J Song, S M Kang, E Kim, C-H Kim, H-T Song, J E Le. Impairment of insulin receptor substrate 1 signaling by insulin resistance inhibits neurite outgrowth and aggravates neuronal cell death. Neuroscience. vol 301. 2016-04-18. PMID:26047734. |
to investigate the changes caused by the inhibition of irs-1-mediated insulin signaling in neuroblastoma cells, we performed western blot analysis, reverse transcription-pcr, and immunochemical analysis. |
2016-04-18 |
2023-08-13 |
mouse |
| Hu-Ping Deng, Jia-Ke Chai, Chuan-An Shen, Xi-Bo Zhang, Li Ma, Tian-Jun Sun, Qing-Gang Hu, Yun-Fei Chi, Ning Don. Insulin down-regulates the expression of ubiquitin E3 ligases partially by inhibiting the activity and expression of AMP-activated protein kinase in L6 myotubes. Bioscience reports. vol 35. issue 4. 2016-04-11. PMID:26193886. |
these treatments were also conducted in the presence or absence of insulin and the cells were analysed by western blot and quantitative real-time pcr. |
2016-04-11 |
2023-08-13 |
Not clear |
| P K Shrestha, K P Brisk. Hindbrain lactate regulates preoptic gonadotropin-releasing hormone (GnRH) neuron GnRH-I protein but not AMPK responses to hypoglycemia in the steroid-primed ovariectomized female rat. Neuroscience. vol 298. 2016-03-21. PMID:25934033. |
western blot analyses of laser-microdissected a2 neurons demonstrate hypoglycemic augmentation [fos, estrogen receptor-beta (er-β), phosphoampk (pampk)] and inhibition (dopamine-beta-hydroxylase, glut3, mct2) of protein expression in these cells, responses that were normalized by insulin plus lactate treatment. |
2016-03-21 |
2023-08-13 |
rat |
| Wenjun Wu, Sunyinyan Tang, Junfeng Shi, Wenwen Yin, Shu Cao, Ruifang Bu, Dalong Zhu, Yan B. Metformin attenuates palmitic acid-induced insulin resistance in L6 cells through the AMP-activated protein kinase/sterol regulatory element-binding protein-1c pathway. International journal of molecular medicine. vol 35. issue 6. 2016-01-25. PMID:25891779. |
l6 muscle cells were incubated with palmitic acid (pa) to induce insulin resistance and then treated with metformin and/or the ampk inhibitor, compound c. ampk, srebp-1c, irs-1 and akt protein expression levels were determined by western blot analysis. |
2016-01-25 |
2023-08-13 |
Not clear |
| De-Lin Ma, Fu-Qiong Chen, Wei-Jie Xu, Wen-Zhu Yue, Gang Yuan, Yan Yan. Early intervention with glucagon-like peptide 1 analog liraglutide prevents tau hyperphosphorylation in diabetic db/db mice. Journal of neurochemistry. vol 135. issue 2. 2016-01-11. PMID:26183127. |
male db/db mice (3-3.5 weeks) were daily injected subcutaneously with liraglutide (n = 27), insulin (n = 27), or saline (n = 26), and five to seven mice were killed every 2 weeks for analysis of plasma and cerebrospinal (csf) insulin levels by elisa, and protein levels in the hippocampal formation by western blot. |
2016-01-11 |
2023-08-13 |
mouse |
| Ou Bian, Haishan Zhang, Qigang Guan, Yingxian Sun, Dingyin Zen. High-dose insulin inhibits gap junction intercellular communication in vascular smooth muscle cells. Molecular medicine reports. vol 12. issue 1. 2015-12-31. PMID:25760256. |
western blot assays further demonstrated that high‑dose insulin induced the phosphorylation of cx43 at s368 and downregulated the expression of cx43. |
2015-12-31 |
2023-08-13 |
Not clear |
| Lina Ma, Zhimin Shao, Rong Wang, Zhiwei Zhao, Xu Zhang, Zhijuan Ji, Shuli Sheng, Baolei Xu, Wen Dong, Jingshuang Zhan. The β-amyloid precursor protein analog P165 improves impaired insulin signal transduction in type 2 diabetic rats. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology. vol 36. issue 4. 2015-12-09. PMID:25380623. |
after 8 weeks of treatment, the animals were killed and the expression of insulin signaling-related proteins was examined in the hippocampus by western blot and immunohistochemical staining. |
2015-12-09 |
2023-08-13 |
rat |
| Gökhan Sadi, Mehmet Bilgehan Pektaş, Halit Bugra Koca, Murat Tosun, Tulay Koc. Resveratrol improves hepatic insulin signaling and reduces the inflammatory response in streptozotocin-induced diabetes. Gene. vol 570. issue 2. 2015-11-17. PMID:26071184. |
in addition to hepatic glucose, lipid, insulin, alt, ast, resistin and xod contents, gene and protein expressions of insulin signaling pathway components such as insulin rβ, irs-1, irs-2, enos, pi3k, akt, and foxo3a were analyzed by qrt-pcr and western blot. |
2015-11-17 |
2023-08-13 |
rat |
| Jennifer L Steiner, Megan E Bardgett, Lawrence Wolfgang, Charles H Lang, Sean D Stocke. Glucocorticoids attenuate the central sympathoexcitatory actions of insulin. Journal of neurophysiology. vol 112. issue 10. 2015-10-19. PMID:25185805. |
western blot analysis indicates insulin produced similar activation of akt ser(473) and rps6 ser(240/244) in the ventral hypothalamus of control and dexamethasone-treated rats. |
2015-10-19 |
2023-08-13 |
rat |
| Lina Ma, Zhimin Shao, Rong Wang, Zhiwei Zhao, Wen Dong, Jingshuang Zhang, Xu Zhang, Shuli Sheng, Zhijuan Ji, Jingyan Zhan. Rosiglitazone improves learning and memory ability in rats with type 2 diabetes through the insulin signaling pathway. The American journal of the medical sciences. vol 350. issue 2. 2015-10-13. PMID:25973687. |
western blot and immunohistochemistry were also used to detect changes in proteins involved in the insulin signal transduction pathway, such as the insulin receptor, insulin receptor substrate-1, protein kinase b, phosphorylated camp response element-binding protein and b-cell lymphoma 2, in the hippocampus of the rats. |
2015-10-13 |
2023-08-13 |
rat |
| Ahmed A Abolliel, Hamdallah Zeda. Synthesis, cloning and expression of a novel pre-miniproinsulin analogue gene in Escherichia coli. Journal of advanced research. vol 6. issue 5. 2015-10-01. PMID:26425357. |
the identity of the expressed protein was verified by immunological detection method of western blot using polyclonal rabbit antibody against insulin. |
2015-10-01 |
2023-08-13 |
human |