| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Manabu Ishiki, Yasuhiro Nishida, Hiroshi Ishibashi, Tsutomu Wada, Shiho Fujisaka, Akiko Takikawa, Masaharu Urakaze, Toshiyasu Sasaoka, Isao Usui, Kazuyuki Tob. Impact of divergent effects of astaxanthin on insulin signaling in L6 cells. Endocrinology. vol 154. issue 8. 2013-10-29. PMID:23715867. |
we examined the effect of astaxanthin on insulin-stimulated glucose transporter 4 (glut4) translocation, glucose uptake, and insulin signaling in cultured rat l6 muscle cells using plasma membrane lawn assay, 2-deoxyglucose uptake, and western blot analysis. |
2013-10-29 |
2023-08-12 |
rat |
| Rongjing Song, Xi Wang, Yiqing Mao, Hui Li, Zhixin Li, Wei Xu, Rong Wang, Tingting Guo, Ling Jin, Xiaojing Zhang, Yizhuang Zhang, Na Zhou, Ruobi Hu, Jianwei Jia, Zhen Lei, David M Irwin, Gang Niu, Huanran Ta. Resistin disrupts glycogen synthesis under high insulin and high glucose levels by down-regulating the hepatic levels of GSK3β. Gene. vol 529. issue 1. 2013-10-29. PMID:23860320. |
protein levels of factors in the insulin signaling pathway involved in glycogen synthesis were examined by western blot analysis, with the only significant change observed being the level of phosphorylated (at ser 9) glycogen synthase kinase-3β (gsk-3β) (p<0.001). |
2013-10-29 |
2023-08-12 |
mouse |
| Antonio J Ruiz-Alcaraz, Christopher Lipina, John R Petrie, Michael J Murphy, Andrew D Morris, Calum Sutherland, Daniel J Cuthbertso. Obesity-induced insulin resistance in human skeletal muscle is characterised by defective activation of p42/p44 MAP kinase. PloS one. vol 8. issue 2. 2013-08-23. PMID:23468892. |
skeletal muscle (vastus lateralis) biopsies were taken before and after one hour of hyperinsulinaemia and the muscle insulin signalling proteins examined by western blot and immunoprecipitation assay. |
2013-08-23 |
2023-08-12 |
human |
| Hongxia Zhang, Junjie Hou, Ruina Cui, Xuejiang Guo, Zhimin Shi, Fuquan Yang, Jiayin Da. Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. Toxicology letters. vol 218. issue 1. 2013-04-16. PMID:23353032. |
western blot and qpcr results suggested that chronic pfdoa exposure inhibited insulin signal pathways and that inhibition of gsk3 might contribute to the observed increases of lipid levels in the liver. |
2013-04-16 |
2023-08-12 |
rat |
| Yue-Chun Wang, Yuan Zhang, A-Lin Duan, Wei-Xia Lin, Qiao-Dan Zheng, Wen-Lu X. [Rapid differentiation of human umbilical cord-derived mesenchymal stem cells into insulin-secreting cells under the sole induction of biological products]. Sheng li xue bao : [Acta physiologica Sinica]. vol 62. issue 1. 2013-01-24. PMID:20179892. |
before and after the induction, the morphological changes were observed under inverse microscope; the islet-related genes were detected by rt-pcr; islet-like clusters (ilcs) were identified by dithizone (dtz) staining; pdx-1 and immunoreactive insulin (iri) were examined by immunofluorescence method; the quantity and quality of iri secretion were assayed by chemiluminescence immunoassay and western blot respectively. |
2013-01-24 |
2023-08-12 |
human |
| Li Wang, Wei Zhou, Shanmiao Gou, Tongling Wang, Tao Liu, Chunyou Wan. Insulin promotes proliferative vitality and invasive capability of pancreatic cancer cells via hypoxia-inducible factor 1alpha pathway. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 30. issue 3. 2013-01-24. PMID:20556580. |
western blot analysis demonstrated that insulin dose-dependently increased the hif-1alpha protein expression, and yc-1 could dose-dependently block this effect. |
2013-01-24 |
2023-08-12 |
human |
| Xiaomei Liu, Ying Qi, Hong Gao, Yisheng Jiao, Hui Gu, Jianing Miao, Zhengwei Yua. Maternal protein restriction induces alterations in insulin signaling and ATP sensitive potassium channel protein in hypothalami of intrauterine growth restriction fetal rats. Journal of clinical biochemistry and nutrition. vol 52. issue 1. 2013-01-24. PMID:23341697. |
the expression and activated levels of insulin signaling molecules and k(atp) protein in the hypothalami which were dissected at 20 days of gestation, were analyzed by western blot and real time pcr. |
2013-01-24 |
2023-08-12 |
rat |
| Yu-Wen Cheng, Ying-I Chen, Chung-Yuh Tzeng, Hong-Chen Chen, Chin-Chun Tsai, Yu-Chen Lee, Jaung-Geng Lin, Yiu-Kay Lai, Shih-Liang Chan. Extracts of Cordyceps militaris lower blood glucose via the stimulation of cholinergic activation and insulin secretion in normal rats. Phytotherapy research : PTR. vol 26. issue 8. 2012-12-04. PMID:22821853. |
further, atropine and hemicholinium-3 (hc-3) were injected and a western blot was used to investigate insulin signaling. |
2012-12-04 |
2023-08-12 |
rat |
| R Oubaassine, M Weckering, L Kessler, M Breidert, J C Roegel, P Eftekhar. Insulin interacts directly with Na⁺/K⁺ATPase and protects from digoxin toxicity. Toxicology. vol 299. issue 1. 2012-10-17. PMID:22562035. |
the interaction of insulin with na⁺/k⁺-atpase was explored using enzyme activity, biacore and western blot. |
2012-10-17 |
2023-08-12 |
rat |
| R Oubaassine, M Weckering, L Kessler, M Breidert, J C Roegel, P Eftekhar. Insulin interacts directly with Na⁺/K⁺ATPase and protects from digoxin toxicity. Toxicology. vol 299. issue 1. 2012-10-17. PMID:22562035. |
in western blot, the same insulin concentration decreased enzyme α subunit immunoreactivity. |
2012-10-17 |
2023-08-12 |
rat |
| Hua-Jun He, Yaping Zong, Michel Bernier, Lili Wan. Sensing the insulin signaling pathway with an antibody array. Proteomics. Clinical applications. vol 3. issue 12. 2012-10-02. PMID:21136963. |
the results of the arrayed antibodies were verified by the multiplexed bead array assay and conventional western blot analysis, and confirmed the well-known inhibitory effects of phorbol esters on insulin signaling pathway activation. |
2012-10-02 |
2023-08-12 |
Not clear |
| Reza Hakkak, Ahmed Al-Dwairi, George J Fuchs, Soheila Korourian, Frank A Simme. Dietary soy protein induces hepatic lipogenic enzyme gene expression while suppressing hepatosteatosis in obese female Zucker rats bearing DMBA-initiated mammary tumors. Genes & nutrition. vol 7. issue 4. 2012-10-02. PMID:22528625. |
western blot analysis showed a significant induction of me1 protein expression in the livers of obese, soy protein-fed rats, which paralleled the increased serum insulin level in this group. |
2012-10-02 |
2023-08-12 |
rat |
| Juan Wang, Yangjun Gao, Yan Lu, Xiaolong Tang, Dongmei He, Yuan Zhan. [Induced differentiation of human umbilical cord mesenchymal stem modified by cells Pdx1gene into islet beta-like cells in vitro]. Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi. vol 28. issue 6. 2012-09-06. PMID:22295709. |
the genes' expressions related to islet beta cells such as pdx1, insulin, glucose transporter-2 (glut2), were detected with rt-pcr, immunocytochemistry and western blot. |
2012-09-06 |
2023-08-12 |
human |
| Yingmei Wang, Yuanxi Zhu, Lizhi Zhang, Wenyan Tian, Shaofang Hua, Jing Zhao, Huiying Zhang, Fengxia Xu. Insulin promotes proliferation, survival, and invasion in endometrial carcinoma by activating the MEK/ERK pathway. Cancer letters. vol 322. issue 2. 2012-08-20. PMID:22459351. |
the involvement of insulin in endometrial carcinoma (ec) was investigated using radioimmunoassay, western blot, immunoprecipitation, mtt, and annexin v-fitc/pi assays in tissue samples and cultured cells. |
2012-08-20 |
2023-08-12 |
Not clear |
| Thiago M Batista, Paloma Alonso-Magdalena, Elaine Vieira, Maria Esmeria C Amaral, Christopher R Cederroth, Serge Nef, Ivan Quesada, Everardo M Carneiro, Angel Nada. Short-term treatment with bisphenol-A leads to metabolic abnormalities in adult male mice. PloS one. vol 7. issue 3. 2012-08-03. PMID:22470480. |
insulin signaling assays were conducted by western blot analysis. |
2012-08-03 |
2023-08-12 |
mouse |
| Jason W Bjork, Lee A Meier, Sandra L Johnson, Zeeshan H Syedain, Robert T Tranquill. Hypoxic culture and insulin yield improvements to fibrin-based engineered tissue. Tissue engineering. Part A. vol 18. issue 7-8. 2012-07-23. PMID:22011014. |
western blot analysis showed that insulin supplementation alone increased akt phosphorylation and the combined treatment increased collagen prolyl-4-hydroxylase. |
2012-07-23 |
2023-08-12 |
human |
| Yinan Hua, Yingmei Zhang, Asli F Ceylan-Isik, Loren E Wold, Jennifer M Nunn, Jun Re. Chronic Akt activation accentuates aging-induced cardiac hypertrophy and myocardial contractile dysfunction: role of autophagy. Basic research in cardiology. vol 106. issue 6. 2012-03-27. PMID:21901288. |
level of insulin signaling and autophagy was evaluated by western blot. |
2012-03-27 |
2023-08-12 |
mouse |
| Eugenio Cersosimo, Xiaojing Xu, Nicolas Mus. Potential role of insulin signaling on vascular smooth muscle cell migration, proliferation, and inflammation pathways. American journal of physiology. Cell physiology. vol 302. issue 4. 2012-03-22. PMID:22094332. |
vsmcs were treated in a dose-response manner with insulin (0, 1, 10, and 100 nm) for 20 min, and akt and erk phosphorylation were measured by western blot analysis. |
2012-03-22 |
2023-08-12 |
human |
| Ji-Hong Liu, Dong-Fang Liu, Nan-Nan Wang, Hai-Ling Lin, Xi Me. Possible role for the thioredoxin system in the protective effects of probucol in the pancreatic islets of diabetic rats. Clinical and experimental pharmacology & physiology. vol 38. issue 8. 2012-01-26. PMID:21615774. |
in addition, the expression of insulin, trx and txnip in islets was analysed using immunohistochemical, western blot and reverse transcription-polymerase chain reaction methods. |
2012-01-26 |
2023-08-12 |
rat |
| Shin Hato. Hormonal regulation of Na+/K+-dependent ATPase activity and pump function in corneal endothelial cells. Cornea. vol 30 Suppl 1. 2012-01-03. PMID:21912233. |
western blot analysis indicated that insulin decreased the amount of inactive na,k-atpase α1-subunit, but the expression of total na,k-atpase α1-subunit was unchanged. |
2012-01-03 |
2023-08-12 |
mouse |