| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Q X Yuan, L P Teng, J Y Zhou, C P Liu, J Guo, L J Liu, W De, C Li. Characterization of Munc13-1 and insulin secretion during pancreatic development in rats. Journal of endocrinological investigation. vol 31. issue 7. 2008-12-22. PMID:18787382. |
the expression of munc13-1 and insulin secretion was studied by the techniques of rtpcr, real-time pcr, western blot, and enzyme-linked immunosorbent assay. |
2008-12-22 |
2023-08-12 |
rat |
| BingZhi Yu, Zhe Zhang, Xin Deng, XiaoYan Xu, Chen Feng, YanXiao Li, Cheng Cui, WenHui Su, HongMei Zhao, DaHai Y. Involvement of insulin in early development of mouse one-cell stage embryos. Science in China. Series C, Life sciences. vol 51. issue 9. 2008-11-04. PMID:18726521. |
in the present study, we examined the expression and localization of insulin in the mouse oocytes and one-cell stage embryos by quantitative elisa, rt-pcr, western blot and immunofluorescence. |
2008-11-04 |
2023-08-12 |
mouse |
| Michael Hsiao, Pei-Jung Lu, Hsiao-Ning Huang, Wan-Chen Lo, Wen-Yu Ho, Tsung-Ching Lai, Hung-Ting Chiang, Ching-Jiunn Tsen. Defective phosphatidylinositol 3-kinase signaling in central control of cardiovascular effects in the nucleus tractus solitarii of spontaneously hypertensive rats. Hypertension research : official journal of the Japanese Society of Hypertension. vol 31. issue 6. 2008-09-09. PMID:18716370. |
western blot analysis showed no significant increase in akt phosphorylation in 8-week-old pre-hypertensive shr after insulin injection. |
2008-09-09 |
2023-08-12 |
rat |
| Christian Freund, Dorien Ward-van Oostwaard, Jantine Monshouwer-Kloots, Stieneke van den Brink, Marga van Rooijen, Xiuqin Xu, Robert Zweigerdt, Christine Mummery, Robert Passie. Insulin redirects differentiation from cardiogenic mesoderm and endoderm to neuroectoderm in differentiating human embryonic stem cells. Stem cells (Dayton, Ohio). vol 26. issue 3. 2008-05-07. PMID:18096723. |
real-time reverse transcription-polymerase chain reaction and western blot analysis showed that insulin inhibited cardiomyogenesis in the early phase of coculture by suppressing the expression of endoderm (foxa2, gata-6), mesoderm (brachyury t), and cardiac mesoderm (nkx2.5, gata-4). |
2008-05-07 |
2023-08-12 |
human |
| Haiyan Liu, Xiaodong Liu, Lee Jia, Yuchun Liu, Huiwen Yang, Guangji Wang, Lin Xi. Insulin therapy restores impaired function and expression of P-glycoprotein in blood-brain barrier of experimental diabetes. Biochemical pharmacology. vol 75. issue 8. 2008-04-22. PMID:18299117. |
in vitro effects of insulin on function and expression of p-gp in primarily cultured rat brain microvessel endothelial cells (rbmecs) were evaluated using rhodamine 123 (rho123) uptakes and western blot, respectively. |
2008-04-22 |
2023-08-12 |
rat |
| N Montserrat, J Sánchez-Gurmaches, D García de la Serrana, M I Navarro, J Gutiérre. IGF-I binding and receptor signal transduction in primary cell culture of muscle cells of gilthead sea bream: changes throughout in vitro development. Cell and tissue research. vol 330. issue 3. 2008-04-11. PMID:17940802. |
the activation of various kinase (erk 1/2 mapk and akt/pkb) proteins by igfs and insulin was studied by means of western blot analysis. |
2008-04-11 |
2023-08-12 |
Not clear |
| Rickard Köhnke, Jie Mei, Miejung Park, David A York, Charlotte Erlanson-Albertsso. Fatty acids and glucose in high concentration down-regulates ATP synthase beta-subunit protein expression in INS-1 cells. Nutritional neuroscience. vol 10. issue 5-6. 2008-03-20. PMID:18284036. |
atp synthase beta-subunit expression was measured by western blot, atp content was monitored by atp luminescence and insulin secretion detected by radio immunoassay. |
2008-03-20 |
2023-08-12 |
Not clear |
| Bolin Qin, Yoshiharu Oshida, Ping Li, Masakazu Kubota, Masaru Nagasaki, Yuzo Sat. Voluntary running improves in vivo insulin resistance in high-salt diet fed rats. Experimental biology and medicine (Maywood, N.J.). vol 232. issue 10. 2008-03-14. PMID:17959846. |
further analyses of the possible changes in insulin signaling occurring in skeletal muscle were performed by western blot and reverse transcription polymerase chain reaction (rt-pcr). |
2008-03-14 |
2023-08-12 |
rat |
| Gefeng Li, Imtiaz S Ali, R William Curri. Insulin-induced myocardial protection in isolated ischemic rat hearts requires p38 MAPK phosphorylation of Hsp27. American journal of physiology. Heart and circulatory physiology. vol 294. issue 1. 2008-02-28. PMID:17906111. |
by 3 h after insulin treatment, a significant increase in hsp70, but not hsp27, was detected by western blot analysis. |
2008-02-28 |
2023-08-12 |
rat |
| Swasti Tiwari, Veerendra K M Halagappa, Shahla Riazi, Xinqun Hu, Carolyn A Ecelbarge. Reduced expression of insulin receptors in the kidneys of insulin-resistant rats. Journal of the American Society of Nephrology : JASN. vol 18. issue 10. 2008-02-25. PMID:17855644. |
using two rat models of insulin resistance, western blot analysis demonstrated a significant reduction in the expression of insulin receptor subunits in the kidney compared to lean control rats. |
2008-02-25 |
2023-08-12 |
rat |
| Jiro Nakamur. Protein kinase C-dependent antilipolysis by insulin in rat adipocytes. Biochimica et biophysica acta. vol 1771. issue 9. 2007-12-27. PMID:17689141. |
western blot analysis revealed that insulin slightly increases membrane-bound pkcalpha, betai, and delta, and wortmannin decreases pkcbetai, betaii, and delta in the membrane fraction. |
2007-12-27 |
2023-08-12 |
rat |
| Noriko Yamabe, Takako Yokozaw. Protective effect of Hachimi-jio-gan against the development of pancreatic fibrosis and oxidative damage in Otsuka Long-Evans Tokushima Fatty rats. Journal of ethnopharmacology. vol 113. issue 1. 2007-11-13. PMID:17606344. |
in addition, pancreatic wet weight, insulin content, and western blot analyses of transforming growth factor-beta(1), fibronectin, and nuclear factor-kappab-related inflammatory enzymes, such as inducible nitric oxide synthesis and cyclooxygenase-2, were also performed in the pancreas. |
2007-11-13 |
2023-08-12 |
rat |
| S M Mense, A Sengupta, M Zhou, C Lan, G Bentsman, D J Volsky, L Zhan. Gene expression profiling reveals the profound upregulation of hypoxia-responsive genes in primary human astrocytes. Physiological genomics. vol 25. issue 3. 2007-11-08. PMID:16507782. |
indeed, western blot analysis confirmed that two major signal transducers mediating insulin and egf action, akt and mek1/2, were activated by hypoxia in astrocytes. |
2007-11-08 |
2023-08-12 |
human |
| Andrea De Gottardi, Manlio Vinciguerra, Antonino Sgroi, Moulay Moukil, Florence Ravier-Dall'Antonia, Valerio Pazienza, Paolo Pugnale, Michelangelo Foti, Antoine Hadengu. Microarray analyses and molecular profiling of steatosis induction in immortalized human hepatocytes. Laboratory investigation; a journal of technical methods and pathology. vol 87. issue 8. 2007-10-19. PMID:17558421. |
finally, quantitative analysis of signalling induced by insulin was performed by western blot. |
2007-10-19 |
2023-08-12 |
human |
| Christine Schäfer, Thor Gehrmann, Lisa Richter, Verena Keitel, Karl Köhrer, Dieter Häussinger, Freimut Schlies. Modulation of gene expression profiles by hyperosmolarity and insulin. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. vol 20. issue 5. 2007-09-27. PMID:17762165. |
here, the modulation of gene expression profiles by hyperosmolarity and insulin was examined in h4iie rat hepatoma cells by cdna/oligonucleotiode array-, northern- and western blot analysis. |
2007-09-27 |
2023-08-12 |
rat |
| Wael M Maharsy, Lina N Kadi, Nahla G Issa, Khalil M Bitar, Asdghig H Der-Boghossian, Roy Abrahamian, Anwar B Bikhaz. Cross-talk related to insulin and angiotensin II binding on myocardial remodelling in diabetic rat hearts. Journal of the renin-angiotensin-aldosterone system : JRAAS. vol 8. issue 2. 2007-09-26. PMID:17703431. |
western blot results for at(1)-receptor density showed an increase in diabetics compared to normal with no normalising effect with insulin treatment. |
2007-09-26 |
2023-08-12 |
rat |
| Amitabha Ray, Katai J Nkhata, Margot P Clear. Effects of leptin on human breast cancer cell lines in relationship to estrogen receptor and HER2 status. International journal of oncology. vol 30. issue 6. 2007-07-12. PMID:17487372. |
leptin was added at 0, 5, 10, 25, 50 and 100 ng/ml for 24 and 48 h. for western blot analyses, protein extracts were probed for ob-rb, ob-r, leptin, jak2, pi3k, stat3, p-stat3, pcna, cyclin d1, cox-2, vegf, bcl-2, bcl-xl, bax, insulin, igf-i, igfbp3, igf-iralpha, aromatase, cyp1a1 and cyp1b1. |
2007-07-12 |
2023-08-12 |
human |
| Ursula A White, Ann A Coulter, Tiffany K Miles, Jacqueline M Stephen. The STAT5A-mediated induction of pyruvate dehydrogenase kinase 4 expression by prolactin or growth hormone in adipocytes. Diabetes. vol 56. issue 6. 2007-06-14. PMID:17360981. |
both northern blot and western blot analyses were conducted on samples isolated from 3t3-l1 adipocytes after various treatments with prolactin (prl), growth hormone (gh), and/or insulin. |
2007-06-14 |
2023-08-12 |
Not clear |
| Eadaoin Mc Kiernan, Niall W Barron, Finbarr O'Sullivan, Paul Barham, Martin Clynes, Lorraine O'Driscol. Detecting de novo insulin synthesis in embryonic stem cell-derived populations. Experimental cell research. vol 313. issue 7. 2007-06-05. PMID:17362926. |
in combination with other techniques, including immunofluorescent staining and western blot analysis to detect and quantify c-peptide, we conclude that the majority of the insulin found in these differentiated es cell cultures is medium-derived. |
2007-06-05 |
2023-08-12 |
Not clear |
| Nichola M Thompson, Amy M Norman, Shawn S Donkin, Ravi R Shankar, Mark H Vickers, Jennifer L Miles, Bernhard H Breie. Prenatal and postnatal pathways to obesity: different underlying mechanisms, different metabolic outcomes. Endocrinology. vol 148. issue 5. 2007-06-04. PMID:17272392. |
real-time pcr and western blot analyses of key markers of insulin sensitivity and metabolic regulation showed that iugr offspring had increased hepatic levels of atypical protein kinase c zeta (pkc zeta) and increased expression of fatty acid synthase mrna. |
2007-06-04 |
2023-08-12 |
rat |