Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
Rickard Köhnke, Jie Mei, Miejung Park, David A York, Charlotte Erlanson-Albertsso. Fatty acids and glucose in high concentration down-regulates ATP synthase beta-subunit protein expression in INS-1 cells. Nutritional neuroscience. vol 10. issue 5-6. 2008-03-20. PMID:18284036. |
atp synthase beta-subunit expression was measured by western blot, atp content was monitored by atp luminescence and insulin secretion detected by radio immunoassay. |
2008-03-20 |
2023-08-12 |
Not clear |
Bolin Qin, Yoshiharu Oshida, Ping Li, Masakazu Kubota, Masaru Nagasaki, Yuzo Sat. Voluntary running improves in vivo insulin resistance in high-salt diet fed rats. Experimental biology and medicine (Maywood, N.J.). vol 232. issue 10. 2008-03-14. PMID:17959846. |
further analyses of the possible changes in insulin signaling occurring in skeletal muscle were performed by western blot and reverse transcription polymerase chain reaction (rt-pcr). |
2008-03-14 |
2023-08-12 |
rat |
Gefeng Li, Imtiaz S Ali, R William Curri. Insulin-induced myocardial protection in isolated ischemic rat hearts requires p38 MAPK phosphorylation of Hsp27. American journal of physiology. Heart and circulatory physiology. vol 294. issue 1. 2008-02-28. PMID:17906111. |
by 3 h after insulin treatment, a significant increase in hsp70, but not hsp27, was detected by western blot analysis. |
2008-02-28 |
2023-08-12 |
rat |
Swasti Tiwari, Veerendra K M Halagappa, Shahla Riazi, Xinqun Hu, Carolyn A Ecelbarge. Reduced expression of insulin receptors in the kidneys of insulin-resistant rats. Journal of the American Society of Nephrology : JASN. vol 18. issue 10. 2008-02-25. PMID:17855644. |
using two rat models of insulin resistance, western blot analysis demonstrated a significant reduction in the expression of insulin receptor subunits in the kidney compared to lean control rats. |
2008-02-25 |
2023-08-12 |
rat |
Jiro Nakamur. Protein kinase C-dependent antilipolysis by insulin in rat adipocytes. Biochimica et biophysica acta. vol 1771. issue 9. 2007-12-27. PMID:17689141. |
western blot analysis revealed that insulin slightly increases membrane-bound pkcalpha, betai, and delta, and wortmannin decreases pkcbetai, betaii, and delta in the membrane fraction. |
2007-12-27 |
2023-08-12 |
rat |
Noriko Yamabe, Takako Yokozaw. Protective effect of Hachimi-jio-gan against the development of pancreatic fibrosis and oxidative damage in Otsuka Long-Evans Tokushima Fatty rats. Journal of ethnopharmacology. vol 113. issue 1. 2007-11-13. PMID:17606344. |
in addition, pancreatic wet weight, insulin content, and western blot analyses of transforming growth factor-beta(1), fibronectin, and nuclear factor-kappab-related inflammatory enzymes, such as inducible nitric oxide synthesis and cyclooxygenase-2, were also performed in the pancreas. |
2007-11-13 |
2023-08-12 |
rat |
S M Mense, A Sengupta, M Zhou, C Lan, G Bentsman, D J Volsky, L Zhan. Gene expression profiling reveals the profound upregulation of hypoxia-responsive genes in primary human astrocytes. Physiological genomics. vol 25. issue 3. 2007-11-08. PMID:16507782. |
indeed, western blot analysis confirmed that two major signal transducers mediating insulin and egf action, akt and mek1/2, were activated by hypoxia in astrocytes. |
2007-11-08 |
2023-08-12 |
human |
Andrea De Gottardi, Manlio Vinciguerra, Antonino Sgroi, Moulay Moukil, Florence Ravier-Dall'Antonia, Valerio Pazienza, Paolo Pugnale, Michelangelo Foti, Antoine Hadengu. Microarray analyses and molecular profiling of steatosis induction in immortalized human hepatocytes. Laboratory investigation; a journal of technical methods and pathology. vol 87. issue 8. 2007-10-19. PMID:17558421. |
finally, quantitative analysis of signalling induced by insulin was performed by western blot. |
2007-10-19 |
2023-08-12 |
human |
Christine Schäfer, Thor Gehrmann, Lisa Richter, Verena Keitel, Karl Köhrer, Dieter Häussinger, Freimut Schlies. Modulation of gene expression profiles by hyperosmolarity and insulin. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. vol 20. issue 5. 2007-09-27. PMID:17762165. |
here, the modulation of gene expression profiles by hyperosmolarity and insulin was examined in h4iie rat hepatoma cells by cdna/oligonucleotiode array-, northern- and western blot analysis. |
2007-09-27 |
2023-08-12 |
rat |
Wael M Maharsy, Lina N Kadi, Nahla G Issa, Khalil M Bitar, Asdghig H Der-Boghossian, Roy Abrahamian, Anwar B Bikhaz. Cross-talk related to insulin and angiotensin II binding on myocardial remodelling in diabetic rat hearts. Journal of the renin-angiotensin-aldosterone system : JRAAS. vol 8. issue 2. 2007-09-26. PMID:17703431. |
western blot results for at(1)-receptor density showed an increase in diabetics compared to normal with no normalising effect with insulin treatment. |
2007-09-26 |
2023-08-12 |
rat |
Amitabha Ray, Katai J Nkhata, Margot P Clear. Effects of leptin on human breast cancer cell lines in relationship to estrogen receptor and HER2 status. International journal of oncology. vol 30. issue 6. 2007-07-12. PMID:17487372. |
leptin was added at 0, 5, 10, 25, 50 and 100 ng/ml for 24 and 48 h. for western blot analyses, protein extracts were probed for ob-rb, ob-r, leptin, jak2, pi3k, stat3, p-stat3, pcna, cyclin d1, cox-2, vegf, bcl-2, bcl-xl, bax, insulin, igf-i, igfbp3, igf-iralpha, aromatase, cyp1a1 and cyp1b1. |
2007-07-12 |
2023-08-12 |
human |
Ursula A White, Ann A Coulter, Tiffany K Miles, Jacqueline M Stephen. The STAT5A-mediated induction of pyruvate dehydrogenase kinase 4 expression by prolactin or growth hormone in adipocytes. Diabetes. vol 56. issue 6. 2007-06-14. PMID:17360981. |
both northern blot and western blot analyses were conducted on samples isolated from 3t3-l1 adipocytes after various treatments with prolactin (prl), growth hormone (gh), and/or insulin. |
2007-06-14 |
2023-08-12 |
Not clear |
Eadaoin Mc Kiernan, Niall W Barron, Finbarr O'Sullivan, Paul Barham, Martin Clynes, Lorraine O'Driscol. Detecting de novo insulin synthesis in embryonic stem cell-derived populations. Experimental cell research. vol 313. issue 7. 2007-06-05. PMID:17362926. |
in combination with other techniques, including immunofluorescent staining and western blot analysis to detect and quantify c-peptide, we conclude that the majority of the insulin found in these differentiated es cell cultures is medium-derived. |
2007-06-05 |
2023-08-12 |
Not clear |
Nichola M Thompson, Amy M Norman, Shawn S Donkin, Ravi R Shankar, Mark H Vickers, Jennifer L Miles, Bernhard H Breie. Prenatal and postnatal pathways to obesity: different underlying mechanisms, different metabolic outcomes. Endocrinology. vol 148. issue 5. 2007-06-04. PMID:17272392. |
real-time pcr and western blot analyses of key markers of insulin sensitivity and metabolic regulation showed that iugr offspring had increased hepatic levels of atypical protein kinase c zeta (pkc zeta) and increased expression of fatty acid synthase mrna. |
2007-06-04 |
2023-08-12 |
rat |
Catarina Pinto Reis, António J Ribeiro, Simone Houng, Francisco Veiga, Ronald J Neufel. Nanoparticulate delivery system for insulin: design, characterization and in vitro/in vivo bioactivity. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. vol 30. issue 5. 2007-05-31. PMID:17280820. |
insulin encapsulation efficiency and in vitro release were determined by bradford protein assay and bioactivity determined in vitro using a newly developed western blot immunoassay and in vivo using wistar diabetic rats. |
2007-05-31 |
2023-08-12 |
rat |
HaiYan Liu, DongMei Zhang, Xiao Xu, XiaoDong Liu, GuangJi Wang, Lin Xie, XiaoYan Pang, Li Li. Attenuated function and expression of P-glycoprotein at blood-brain barrier and increased brain distribution of phenobarbital in streptozotocin-induced diabetic mice. European journal of pharmacology. vol 561. issue 1-3. 2007-05-22. PMID:17349620. |
the results showed that the brain-to-plasma concentration ratio value of rodamine123 in diabetic mice was significantly higher than that of non-diabetic mice, western blot suggested that the protein level of p-glycoprotein in the brain of 3-week diabetic mice was significantly lower than that of non-diabetic mice, and insulin treatment restored the impairment of p-glycoprotein. |
2007-05-22 |
2023-08-12 |
mouse |
Sachin Paranjape, Kamlesh Vavaiya, Ajay Kale, Karen Brisk. Role of dorsal vagal motor nucleus orexin-receptor-1 in glycemic responses to acute versus repeated insulin administration. Neuropeptides. vol 41. issue 2. 2007-05-16. PMID:17276508. |
effects of acute versus repeated insulin administration on orx-a and or-1 protein levels in the microdissected dorsal vagal complex (dvc) were evaluated by radioimmunoassay and western blot analyses, respectively. |
2007-05-16 |
2023-08-12 |
rat |
Olga Hrytsenko, James R Wright, Carol M Morrison, Bill Pohajda. Insulin expression in the brain and pituitary cells of tilapia (Oreochromis niloticus). Brain research. vol 1135. issue 1. 2007-04-05. PMID:17196948. |
furthermore, insulin mrna and immunoreactive insulin-like protein was detected in the pituitary gland using in situ hybridization, immunohistochemistry, and western blot analysis. |
2007-04-05 |
2023-08-12 |
Not clear |
Tayaramma Thatava, Thatava Tayaramma, Bin Ma, Manfred Rohde, Hubert Maye. Chromatin-remodeling factors allow differentiation of bone marrow cells into insulin-producing cells. Stem cells (Dayton, Ohio). vol 24. issue 12. 2007-02-08. PMID:16990588. |
western blot analysis demonstrated the presence of stored insulin. |
2007-02-08 |
2023-08-12 |
Not clear |
Esther Velázquez, Antonio Santos, Agustin Montes, Enrique Blázquez, Juan Miguel Ruiz-Albusa. 25-Hydroxycholesterol has a dual effect on the proliferation of cultured rat astrocytes. Neuropharmacology. vol 51. issue 2. 2006-12-04. PMID:16678217. |
finally, in media with albumin alone or in the presence of fetal bovine serum, growth factors, insulin or forskolin, 25-oh-cholesterol did not affect the expression of either c-fos mrna or c-fos protein, as measured by real-time quantitative pcr or by western blot, respectively. |
2006-12-04 |
2023-08-12 |
rat |