| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Abdul Jaleel, Panagiotis Halvatsiotis, Brian Williamson, Peter Juhasz, Stephen Martin, K Sreekumaran Nai. Identification of Amadori-modified plasma proteins in type 2 diabetes and the effect of short-term intensive insulin treatment. Diabetes care. vol 28. issue 3. 2005-06-30. PMID:15735202. |
the objective of the current study was to utilize two-dimensional gel electrophoresis, western blot, and mass spectrometry to identify amadori-modified plasma proteins in type 2 diabetic patients with poor glycemic control and assess the impact of short-term insulin treatment on the glycation of these proteins. |
2005-06-30 |
2023-08-12 |
Not clear |
| Célia Lopes Da Costa, Marta Sampaio De Freitas, Anibal Sanchez Mour. Insulin secretion and GLUT-2 expression in undernourished neonate rats. The Journal of nutritional biochemistry. vol 15. issue 4. 2005-06-24. PMID:15068817. |
glucose-induced insulin secretion by pancreatic islets was analyzed by radioimmunoassay and glut-2 expression in beta-cells by western blot. |
2005-06-24 |
2023-08-12 |
rat |
| L Zhao, Z Li, M Kullin, L A H Borg, F A Karlsso. Alterations in net glucose uptake and in the pancreatic B-cell GLUT2 transporter induced by diazoxide and by secretory stimuli. The Journal of endocrinology. vol 185. issue 2. 2005-06-16. PMID:15845921. |
islet glut2 and insulin were determined by quantitative immunohistochemistry and glut2 was also determined by western blot analysis. |
2005-06-16 |
2023-08-12 |
rat |
| Anita M Hennige, Rainer Lehmann, Cora Weigert, Klaus Moeschel, Myriam Schäuble, Elisabeth Metzinger, Reiner Lammers, Hans-Ulrich Härin. Insulin glulisine: insulin receptor signaling characteristics in vivo. Diabetes. vol 54. issue 2. 2005-04-11. PMID:15677493. |
c57bl/6 mice were injected with insulin glulisine or regular insulin and western blot analysis was performed for liver and muscle tissue. |
2005-04-11 |
2023-08-12 |
mouse |
| Edward A Carter, Deborah Burks, Alan J Fischman, Morris White, Ronald G Tompkin. Insulin resistance in thermally-injured rats is associated with post-receptor alterations in skeletal muscle, liver and adipose tissue. International journal of molecular medicine. vol 14. issue 4. 2005-03-28. PMID:15375597. |
at 3 weeks after injury, glucose production by the liver and utilization by skeletal muscle was measured under insulin clamp conditions, insulin receptor binding was measured in skeletal muscle, liver and adipose tissue membranes and irs-1 expression was measured by western blot methods. |
2005-03-28 |
2023-08-12 |
rat |
| Marianne S Anderson, M Thamotharan, Doris Kao, Sherin U Devaskar, Liping Qiao, Jacob E Friedman, William W Ha. Effects of acute hyperinsulinemia on insulin signal transduction and glucose transporters in ovine fetal skeletal muscle. American journal of physiology. Regulatory, integrative and comparative physiology. vol 288. issue 2. 2005-02-16. PMID:15539611. |
serial skeletal muscle biopsies were taken from each fetus during the clamp and assayed by western blot for selected insulin signal transduction proteins. |
2005-02-16 |
2023-08-12 |
Not clear |
| Hideo Kanehara, Jinya Suzuki, Yasuo Zenimaru, Sadao Takahashi, Koji Oida, Wen-Jun Shen, Fredric B Kraemer, Isamu Miyamor. Function of hormone-sensitive lipase in diacylglycerol-protein kinase C pathway. Diabetes research and clinical practice. vol 65. issue 3. 2005-02-11. PMID:15331200. |
western blot analysis showed that membrane-bound protein kinase c (pkc)-alpha and -epsilon were decreased 40-50% in cells expressing wt-hsl grown in high glucose with insulin. |
2005-02-11 |
2023-08-12 |
rat |
| Phuong Mai Le, Ali Benhaddou-Andaloussi, Aziz Elimadi, Abdellatif Settaf, Yahia Cherrah, Pierre S Hadda. The petroleum ether extract of Nigella sativa exerts lipid-lowering and insulin-sensitizing actions in the rat. Journal of ethnopharmacology. vol 94. issue 2-3. 2005-01-14. PMID:15325727. |
response to insulin was evaluated in hepatocytes isolated from animals of all groups by western blot analysis of phosphorylated mapk p44/42erk and pkb. |
2005-01-14 |
2023-08-12 |
rat |
| Etto C Eringa, Coen D A Stehouwer, Geerten P van Nieuw Amerongen, Lenneke Ouwehand, Nico Westerhof, Pieter Sipkem. Vasoconstrictor effects of insulin in skeletal muscle arterioles are mediated by ERK1/2 activation in endothelium. American journal of physiology. Heart and circulatory physiology. vol 287. issue 5. 2004-11-24. PMID:15059773. |
effects of insulin on erk1/2 activation in arterioles were then investigated by western blot analysis. |
2004-11-24 |
2023-08-12 |
human |
| Naoyuki Yamada, Shinichi Ozawa, Naoko Kageyama, Hiroshi Miyan. Detection and quantification of protein residues in food grade amino acids and nucleic acids using a dot-blot fluorescent staining method. Journal of agricultural and food chemistry. vol 52. issue 17. 2004-10-07. PMID:15315365. |
several standard proteins, such as bovine serum albumin (66 kda), lysozyme (14 kda), ubiquitin (8.6 kda), bovine insulin (5.7 kda), and oxidized insulin b chain (3.5 kda), were detectable at 0.1 ppm using sypro ruby blot stain. |
2004-10-07 |
2023-08-12 |
cattle |
| Michal M Masternak, Khalid Al-Regaiey, Michael S Bonkowski, Jacob Panici, Liou Sun, Jian Wang, Grzegorz K Przybylski, Andrzej Bartk. Divergent effects of caloric restriction on gene expression in normal and long-lived mice. The journals of gerontology. Series A, Biological sciences and medical sciences. vol 59. issue 8. 2004-10-05. PMID:15345726. |
using real-time polymerase chain reaction and western blot, we have examined the expression of genes related to insulin signaling in the liver of normal and dwarf mice subjected to 30% cr. |
2004-10-05 |
2023-08-12 |
mouse |
| Jeltje R Goudriaan, Vivian E H Dahlmans, Bas Teusink, D Margriet Ouwens, Maria Febbraio, J Anton Maassen, Johannes A Romijn, Louis M Havekes, Peter J Vosho. CD36 deficiency increases insulin sensitivity in muscle, but induces insulin resistance in the liver in mice. Journal of lipid research. vol 44. issue 12. 2004-07-01. PMID:12923231. |
moreover, hepatic activation of protein kinase b by insulin, measured by western blot, was reduced by 54%. |
2004-07-01 |
2023-08-12 |
mouse |
| Bo-Chul Shin, Robert A McKnight, Sherin U Devaska. Glucose transporter GLUT8 translocation in neurons is not insulin responsive. Journal of neuroscience research. vol 75. issue 6. 2004-05-03. PMID:14994344. |
by employing stable transfectants that expressed glut8-gfp, the effect of insulin and insulin-like growth factor-i, potassium chloride (depolarized state), and 3% oxygen on translocation of glut8 to the plasma membrane of n2a cells was examined immunohistochemically and by subfractionation, followed by western blot analysis. |
2004-05-03 |
2023-08-12 |
Not clear |
| Tung H Ngo, R James Barnard, Pinchas Cohen, Stephen Freedland, Chris Tran, Frank deGregorio, Yahya I Elshimali, David Heber, William J Aronso. Effect of isocaloric low-fat diet on human LAPC-4 prostate cancer xenografts in severe combined immunodeficient mice and the insulin-like growth factor axis. Clinical cancer research : an official journal of the American Association for Cancer Research. vol 9. issue 7. 2004-04-20. PMID:12855654. |
lf mice had significantly lower levels of serum insulin, tumor igf-1 mrna expression, and tumor igfbp-2 immunostaining and higher levels of serum igfbp-1 (by western ligand blot) relative to the hf mice. |
2004-04-20 |
2023-08-12 |
mouse |
| S M de la Monte, G J Chen, E Rivera, J R Wand. Neuronal thread protein regulation and interaction with microtubule-associated proteins in SH-Sy5y neuronal cells. Cellular and molecular life sciences : CMLS. vol 60. issue 12. 2004-02-11. PMID:14685691. |
quantitative real-time reverse transcription-polymerase chain reactin and western blot analysis demonstrated prominent insulin, moderate insulin-like growth factor, type 1 (igf-1) and minimal nerve growth factor stimulation of ntp expression. |
2004-02-11 |
2023-08-12 |
human |
| D C Wright, C A Fick, J B Olesen, K Lim, B R Barnes, B W Crai. A role for calcium/calmodulin kinase in insulin stimulated glucose transport. Life sciences. vol 74. issue 7. 2004-02-10. PMID:14659970. |
to more accurately determine the interaction between camk and l-type calcium channels, we measured insulin induced changes in camkii phosphorylation using western blot analysis. |
2004-02-10 |
2023-08-12 |
rat |
| Yun-Ping Zhou, Kimberly Marlen, John F Palma, Anthony Schweitzer, Linda Reilly, Francine M Gregoire, Gann G Xu, John E Blume, Jeffrey D Johnso. Overexpression of repressive cAMP response element modulators in high glucose and fatty acid-treated rat islets. A common mechanism for glucose toxicity and lipotoxicity? The Journal of biological chemistry. vol 278. issue 51. 2004-01-30. PMID:14534319. |
northern blot analysis revealed a 5-fold increase in the abundance of crem-17x mrna and a concomitant 50% reduction in the insulin mrna in ffa-treated islets. |
2004-01-30 |
2023-08-12 |
rat |
| Sif G Rønn, Johnny A Hansen, Karen Lindberg, Allan E Karlsen, Nils Billestru. The effect of suppressor of cytokine signaling 3 on GH signaling in beta-cells. Molecular endocrinology (Baltimore, Md.). vol 16. issue 9. 2003-12-19. PMID:12198248. |
furthermore, using northern blot analysis it was shown that socs-3 can completely inhibit gh-induced insulin production in these cells. |
2003-12-19 |
2023-08-12 |
Not clear |
| Serhat Aytug, David Reich, Lawrence E Sapiro, David Bernstein, Najma Begu. Impaired IRS-1/PI3-kinase signaling in patients with HCV: a mechanism for increased prevalence of type 2 diabetes. Hepatology (Baltimore, Md.). vol 38. issue 6. 2003-12-18. PMID:14647049. |
liver tissues were exposed to insulin and examined for the contents and phosphorylation/activation status of the upstream insulin signaling molecules by immunoprecipitation and western blot analysis. |
2003-12-18 |
2023-08-12 |
human |
| Jean Morisset, Jean Lainé, Judith Bourassa, Michel Lessard, Véronique Rome, Paul Guillotea. Presence and localization of CCK receptor subtypes in calf pancreas. Regulatory peptides. vol 111. issue 1-3. 2003-11-21. PMID:12609756. |
using specific antibodies against ccka and cckb receptors, somatostatin, glucagon and insulin, we were able to confirm by western blot the presence of both cck receptor protein subtypes in the calf pancreas as a 80-85-kda ccka receptor and 40-45-kda cckb receptor. |
2003-11-21 |
2023-08-12 |
human |