| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Hideo Kanehara, Jinya Suzuki, Yasuo Zenimaru, Sadao Takahashi, Koji Oida, Wen-Jun Shen, Fredric B Kraemer, Isamu Miyamor. Function of hormone-sensitive lipase in diacylglycerol-protein kinase C pathway. Diabetes research and clinical practice. vol 65. issue 3. 2005-02-11. PMID:15331200. |
western blot analysis showed that membrane-bound protein kinase c (pkc)-alpha and -epsilon were decreased 40-50% in cells expressing wt-hsl grown in high glucose with insulin. |
2005-02-11 |
2023-08-12 |
rat |
| Phuong Mai Le, Ali Benhaddou-Andaloussi, Aziz Elimadi, Abdellatif Settaf, Yahia Cherrah, Pierre S Hadda. The petroleum ether extract of Nigella sativa exerts lipid-lowering and insulin-sensitizing actions in the rat. Journal of ethnopharmacology. vol 94. issue 2-3. 2005-01-14. PMID:15325727. |
response to insulin was evaluated in hepatocytes isolated from animals of all groups by western blot analysis of phosphorylated mapk p44/42erk and pkb. |
2005-01-14 |
2023-08-12 |
rat |
| Etto C Eringa, Coen D A Stehouwer, Geerten P van Nieuw Amerongen, Lenneke Ouwehand, Nico Westerhof, Pieter Sipkem. Vasoconstrictor effects of insulin in skeletal muscle arterioles are mediated by ERK1/2 activation in endothelium. American journal of physiology. Heart and circulatory physiology. vol 287. issue 5. 2004-11-24. PMID:15059773. |
effects of insulin on erk1/2 activation in arterioles were then investigated by western blot analysis. |
2004-11-24 |
2023-08-12 |
human |
| Naoyuki Yamada, Shinichi Ozawa, Naoko Kageyama, Hiroshi Miyan. Detection and quantification of protein residues in food grade amino acids and nucleic acids using a dot-blot fluorescent staining method. Journal of agricultural and food chemistry. vol 52. issue 17. 2004-10-07. PMID:15315365. |
several standard proteins, such as bovine serum albumin (66 kda), lysozyme (14 kda), ubiquitin (8.6 kda), bovine insulin (5.7 kda), and oxidized insulin b chain (3.5 kda), were detectable at 0.1 ppm using sypro ruby blot stain. |
2004-10-07 |
2023-08-12 |
cattle |
| Michal M Masternak, Khalid Al-Regaiey, Michael S Bonkowski, Jacob Panici, Liou Sun, Jian Wang, Grzegorz K Przybylski, Andrzej Bartk. Divergent effects of caloric restriction on gene expression in normal and long-lived mice. The journals of gerontology. Series A, Biological sciences and medical sciences. vol 59. issue 8. 2004-10-05. PMID:15345726. |
using real-time polymerase chain reaction and western blot, we have examined the expression of genes related to insulin signaling in the liver of normal and dwarf mice subjected to 30% cr. |
2004-10-05 |
2023-08-12 |
mouse |
| Jeltje R Goudriaan, Vivian E H Dahlmans, Bas Teusink, D Margriet Ouwens, Maria Febbraio, J Anton Maassen, Johannes A Romijn, Louis M Havekes, Peter J Vosho. CD36 deficiency increases insulin sensitivity in muscle, but induces insulin resistance in the liver in mice. Journal of lipid research. vol 44. issue 12. 2004-07-01. PMID:12923231. |
moreover, hepatic activation of protein kinase b by insulin, measured by western blot, was reduced by 54%. |
2004-07-01 |
2023-08-12 |
mouse |
| Bo-Chul Shin, Robert A McKnight, Sherin U Devaska. Glucose transporter GLUT8 translocation in neurons is not insulin responsive. Journal of neuroscience research. vol 75. issue 6. 2004-05-03. PMID:14994344. |
by employing stable transfectants that expressed glut8-gfp, the effect of insulin and insulin-like growth factor-i, potassium chloride (depolarized state), and 3% oxygen on translocation of glut8 to the plasma membrane of n2a cells was examined immunohistochemically and by subfractionation, followed by western blot analysis. |
2004-05-03 |
2023-08-12 |
Not clear |
| Tung H Ngo, R James Barnard, Pinchas Cohen, Stephen Freedland, Chris Tran, Frank deGregorio, Yahya I Elshimali, David Heber, William J Aronso. Effect of isocaloric low-fat diet on human LAPC-4 prostate cancer xenografts in severe combined immunodeficient mice and the insulin-like growth factor axis. Clinical cancer research : an official journal of the American Association for Cancer Research. vol 9. issue 7. 2004-04-20. PMID:12855654. |
lf mice had significantly lower levels of serum insulin, tumor igf-1 mrna expression, and tumor igfbp-2 immunostaining and higher levels of serum igfbp-1 (by western ligand blot) relative to the hf mice. |
2004-04-20 |
2023-08-12 |
mouse |
| S M de la Monte, G J Chen, E Rivera, J R Wand. Neuronal thread protein regulation and interaction with microtubule-associated proteins in SH-Sy5y neuronal cells. Cellular and molecular life sciences : CMLS. vol 60. issue 12. 2004-02-11. PMID:14685691. |
quantitative real-time reverse transcription-polymerase chain reactin and western blot analysis demonstrated prominent insulin, moderate insulin-like growth factor, type 1 (igf-1) and minimal nerve growth factor stimulation of ntp expression. |
2004-02-11 |
2023-08-12 |
human |
| D C Wright, C A Fick, J B Olesen, K Lim, B R Barnes, B W Crai. A role for calcium/calmodulin kinase in insulin stimulated glucose transport. Life sciences. vol 74. issue 7. 2004-02-10. PMID:14659970. |
to more accurately determine the interaction between camk and l-type calcium channels, we measured insulin induced changes in camkii phosphorylation using western blot analysis. |
2004-02-10 |
2023-08-12 |
rat |
| Yun-Ping Zhou, Kimberly Marlen, John F Palma, Anthony Schweitzer, Linda Reilly, Francine M Gregoire, Gann G Xu, John E Blume, Jeffrey D Johnso. Overexpression of repressive cAMP response element modulators in high glucose and fatty acid-treated rat islets. A common mechanism for glucose toxicity and lipotoxicity? The Journal of biological chemistry. vol 278. issue 51. 2004-01-30. PMID:14534319. |
northern blot analysis revealed a 5-fold increase in the abundance of crem-17x mrna and a concomitant 50% reduction in the insulin mrna in ffa-treated islets. |
2004-01-30 |
2023-08-12 |
rat |
| Sif G Rønn, Johnny A Hansen, Karen Lindberg, Allan E Karlsen, Nils Billestru. The effect of suppressor of cytokine signaling 3 on GH signaling in beta-cells. Molecular endocrinology (Baltimore, Md.). vol 16. issue 9. 2003-12-19. PMID:12198248. |
furthermore, using northern blot analysis it was shown that socs-3 can completely inhibit gh-induced insulin production in these cells. |
2003-12-19 |
2023-08-12 |
Not clear |
| Serhat Aytug, David Reich, Lawrence E Sapiro, David Bernstein, Najma Begu. Impaired IRS-1/PI3-kinase signaling in patients with HCV: a mechanism for increased prevalence of type 2 diabetes. Hepatology (Baltimore, Md.). vol 38. issue 6. 2003-12-18. PMID:14647049. |
liver tissues were exposed to insulin and examined for the contents and phosphorylation/activation status of the upstream insulin signaling molecules by immunoprecipitation and western blot analysis. |
2003-12-18 |
2023-08-12 |
human |
| Jean Morisset, Jean Lainé, Judith Bourassa, Michel Lessard, Véronique Rome, Paul Guillotea. Presence and localization of CCK receptor subtypes in calf pancreas. Regulatory peptides. vol 111. issue 1-3. 2003-11-21. PMID:12609756. |
using specific antibodies against ccka and cckb receptors, somatostatin, glucagon and insulin, we were able to confirm by western blot the presence of both cck receptor protein subtypes in the calf pancreas as a 80-85-kda ccka receptor and 40-45-kda cckb receptor. |
2003-11-21 |
2023-08-12 |
human |
| Kazuya Yamada, Hiroko Kawata, Zhangfei Shou, Tetsuya Mizutani, Tamio Noguchi, Kaoru Miyamot. Insulin induces the expression of the SHARP-2/Stra13/DEC1 gene via a phosphoinositide 3-kinase pathway. The Journal of biological chemistry. vol 278. issue 33. 2003-11-10. PMID:12796501. |
northern blot analysis revealed that the levels of sharp-2 mrna increase when a high carbohydrate diet is fed to normal rats or when insulin is administered to diabetic rats. |
2003-11-10 |
2023-08-12 |
rat |
| D Spampinato, A Giaccari, V Trischitta, B V Costanzo, L Morviducci, A Buongiorno, U Di Mario, R Vigneri, L Frittitt. Rats that are made insulin resistant by glucosamine treatment have impaired skeletal muscle insulin receptor phosphorylation. Metabolism: clinical and experimental. vol 52. issue 9. 2003-10-16. PMID:14506612. |
ir autophosphorylation was measured in skeletal muscle after in vivo insulin stimulation (ie, during clamp) by western blot and then retested after subsequent in vitro 0.1 to 100 nmol/l insulin stimulation (by enzyme-linked immunosorbent assay [elisa]). |
2003-10-16 |
2023-08-12 |
rat |
| D C Wright, C A Fick, J B Olesen, B W Crai. Evidence for the involvement of a phospholipase C--protein kinase C signaling pathway in insulin stimulated glucose transport in skeletal muscle. Life sciences. vol 73. issue 1. 2003-05-29. PMID:12726887. |
western blot analysis revealed that insulin induced a redistribution of pkc beta ii from the cytosol to the membrane that was reversed in the presence of gf109203x (1 microm) and u73122 (20 microm). |
2003-05-29 |
2023-08-12 |
rat |
| Jing He, M Thamotharan, Sherin U Devaska. Insulin-induced translocation of facilitative glucose transporters in fetal/neonatal rat skeletal muscle. American journal of physiology. Regulatory, integrative and comparative physiology. vol 284. issue 4. 2003-04-17. PMID:12531786. |
we examined the effect of insulin on fetal/neonatal rat skeletal muscle glut-1 and glut-4 concentrations and subcellular distribution by employing immunohistochemical analysis and subcellular fractionation followed by western blot analysis. |
2003-04-17 |
2023-08-12 |
rat |
| C Menzaghi, R Di Paola, G Baj, A Funaro, A Arnulfo, T Ercolino, N Surico, F Malavasi, V Trischitt. Insulin modulates PC-1 processing and recruitment in cultured human cells. American journal of physiology. Endocrinology and metabolism. vol 284. issue 3. 2003-03-21. PMID:12441313. |
moreover, at western blot, insulin elicited the appearance, in both plasma membrane and cytosol, of a pc-1-related 146-kda band (in addition to bands of 163, 117, 106, and 97 kda observed also in absence of insulin) that was sensitive to endoglycosidase h. finally, inhibition of pc-1 translocation to plasma membrane, by wortmannin pretreatment, increases insulin-stimulated receptor autophosphorylation. |
2003-03-21 |
2023-08-12 |
human |
| Arshag D Mooradian, Michael J Haas, Joe Chehade, Norman C W Won. Apolipoprotein A-I expression in rats is not altered by troglitazone. Experimental biology and medicine (Maywood, N.J.). vol 227. issue 11. 2003-01-16. PMID:12486210. |
to determine if enhancement of insulin action with insulin sensitizers can also increase the apoa-i expression, we studied the in vivo effect of troglitazone, a potent insulin sensitizer, on the expression of rat hepatic and intestinal apoa-i mrna using northern blot analysis. |
2003-01-16 |
2023-08-12 |
rat |