| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| R Hernandez, T Teruel, M Lorenz. Akt mediates insulin induction of glucose uptake and up-regulation of GLUT4 gene expression in brown adipocytes. FEBS letters. vol 494. issue 3. 2001-05-17. PMID:11311245. |
in consequence, ml-9 precluded insulin stimulation of glucose uptake and glut4 translocation to plasma membrane (determined by western blot), without any effect on the basal glucose uptake. |
2001-05-17 |
2023-08-12 |
rat |
| X Pan, S S Solomon, D M Borromeo, A Martinez-Hernandez, R Ragho. Insulin deprivation leads to deficiency of Sp1 transcription factor in H-411E hepatoma cells and in streptozotocin-induced diabetic ketoacidosis in the rat. Endocrinology. vol 142. issue 4. 2001-04-26. PMID:11250945. |
we demonstrated in rat liver tissue by both western blot and immunohistochemical staining with anti-sp1 antibody that 1) livers of fully diabetic streptozotocin rats have low levels of sp1 transcription factor; and 2) insulin treatment of the diabetic rat rapidly reversed this process by markedly stimulating accumulation of sp1 in rat liver. |
2001-04-26 |
2023-08-12 |
rat |
| G R Devi, D H Yang, R G Rosenfeld, Y O. Differential effects of insulin-like growth factor (IGF)-binding protein-3 and its proteolytic fragments on ligand binding, cell surface association, and IGF-I receptor signaling. Endocrinology. vol 141. issue 11. 2000-12-22. PMID:11089550. |
based on the ascertained and predicted recognition sites for known igfbp-3 proteases, flag-epitope tagged intact igfbp-3, nh2-terminal (1-97), intermediate fragment (88-148), and cooh-terminal fragments (98-264) and (184-264) were generated in a baculovirus and/or escherichia coli expression system and examined, by western ligand blot and affinity cross-linking assays, for their ability to bind igf and insulin. |
2000-12-22 |
2023-08-12 |
Not clear |
| T Utsugi, T Ohno, Y Ohyama, T Uchiyama, Y Saito, Y Matsumura, H Aizawa, H Itoh, M Kurabayashi, S Kawazu, S Tomono, Y Oka, T Suga, M Kuro-o, Y Nabeshima, R Naga. Decreased insulin production and increased insulin sensitivity in the klotho mutant mouse, a novel animal model for human aging. Metabolism: clinical and experimental. vol 49. issue 9. 2000-10-12. PMID:11016890. |
decreased insulin production was also supported by northern blot analysis showing lower levels of insulin mrna in kl/kl mice. |
2000-10-12 |
2023-08-12 |
mouse |
| K Handoko, K Yang, B Strutt, W Khalil, D Killinge. Insulin attenuates the stimulatory effects of tumor necrosis factor alpha on 11beta-hydroxysteroid dehydrogenase 1 in human adipose stromal cells. The Journal of steroid biochemistry and molecular biology. vol 72. issue 3-4. 2000-08-25. PMID:10775808. |
northern blot analysis revealed corresponding changes in the level of 11beta-hsd1 mrna, suggesting that the effects of tnfalpha and insulin on 11beta-hsd1 activity are mediated at the level of gene transcription. |
2000-08-25 |
2023-08-12 |
human |
| Y Ding, N D Vaziri, R Coulson, V S Kamanna, D D Ro. Effects of simulated hyperglycemia, insulin, and glucagon on endothelial nitric oxide synthase expression. American journal of physiology. Endocrinology and metabolism. vol 279. issue 1. 2000-08-08. PMID:10893317. |
the rates of basal and bradykinin-stimulated no production (nitrate + nitrite) and enos protein expression (western blot) were then determined at the basal condition and in the presence of insulin (10(-8) and 10(-7) m), glucagon (10(-8) and 10(-7) m), or both. |
2000-08-08 |
2023-08-12 |
human |
| S Jormsjö, S Ye, J Moritz, D H Walter, S Dimmeler, A M Zeiher, A Henney, A Hamsten, P Eriksso. Allele-specific regulation of matrix metalloproteinase-12 gene activity is associated with coronary artery luminal dimensions in diabetic patients with manifest coronary artery disease. Circulation research. vol 86. issue 9. 2000-06-14. PMID:10807873. |
furthermore, western blot analysis demonstrated that insulin increased mmp-12 protein production. |
2000-06-14 |
2023-08-12 |
Not clear |
| L Yau, H Lukes, H McDiarmid, J Werner, P Zahradk. Insulin-like growth factor-I (IGF-I)-dependent activation of pp42/44 mitogen-activated protein kinase occurs independently of IGF-I receptor kinase activation and IRS-1 tyrosine phosphorylation. European journal of biochemistry. vol 266. issue 3. 2000-01-31. PMID:10583412. |
western blot analysis demonstrated that tyrosine phosphorylation patterns produced by insulin and igf-i differed. |
2000-01-31 |
2023-08-12 |
Not clear |
| C Deachapunya, M Palmer-Densmore, S M O'Grad. Insulin stimulates transepithelial sodium transport by activation of a protein phosphatase that increases Na-K ATPase activity in endometrial epithelial cells. The Journal of general physiology. vol 114. issue 4. 1999-11-19. PMID:10498674. |
immunohistochemical localization along with western blot analysis of cultured porcine endometrial epithelial cells revealed the presence of alpha-1 and alpha-2 isoforms, but not the alpha-3 isoform of na(+)-k(+) atpase, which did not change in the presence of insulin. |
1999-11-19 |
2023-08-12 |
Not clear |
| T H Elsasser, S Kahl, A Martínez, L M Montuenga, R Pio, F Cuttitt. Adrenomedullin binding protein in the plasma of multiple species: characterization by radioligand blotting. Endocrinology. vol 140. issue 10. 1999-10-12. PMID:10499551. |
a series of peptide fragments of am representing amino- and carboxyterminal regions of the hormone, or amylin, calcitonin gene-related peptide (cgrp), or insulin failed to displace intact 125i-am from ligand blot bands. |
1999-10-12 |
2023-08-12 |
mouse |
| F Pacifico, D Liguoro, R Acquaviva, S Formisano, E Consigli. Thyroglobulin binding and TSH regulation of the RHL-1 subunit of the asialoglycoprotein receptor in rat thyroid. Biochimie. vol 81. issue 5. 1999-09-02. PMID:10403180. |
moreover, rt-pcr and western blot assays show that tsh deprivation decreases rhl-1 expression in pc c13 thyroid differentiated cells whereas insulin deprivation does not have any effect. |
1999-09-02 |
2023-08-12 |
rat |
| C Benes, V Poitout, J C Marie, J Martin-Perez, M P Roisin, R Fagar. Mode of regulation of the extracellular signal-regulated kinases in the pancreatic beta-cell line MIN6 and their implication in the regulation of insulin gene transcription. The Biochemical journal. vol 340 ( Pt 1). 1999-06-30. PMID:10229678. |
this was observed by analysis of the transfected rat insulin i gene promoter activity and a northern blot of endogenous insulin mrna. |
1999-06-30 |
2023-08-12 |
rat |
| J E Friedman, T Ishizuka, S Liu, C J Farrell, R J Koletsky, D Bedol, P Ernsberge. Anti-hyperglycemic activity of moxonidine: metabolic and molecular effects in obese spontaneously hypertensive rats. Blood pressure. Supplement. vol 3. 1999-06-24. PMID:10321453. |
western blot analysis of insulin signaling proteins showed that irs-1 was decreased 42% in control shrob compared with shr. |
1999-06-24 |
2023-08-12 |
human |
| E Sciullo, G Cardellini, M G Baroni, P Torresi, A Buongiorno, P Pozzilli, F Fallucc. Glucose transporter (Glut1, Glut3) mRNA in human placenta of diabetic and non-diabetic pregnancies. Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy. vol 3. issue 3. 1999-04-15. PMID:10086067. |
we therefore investigated placental glut1 and glut3 mrna by northern blot analysis in ten diabetic (five insulin dependent diabetes mellitus (iddm), two non-insulin dependent diabetes mellitus (niddm) and three gestational diabetes mellitus (gdm)) and nine non-diabetic women. |
1999-04-15 |
2023-08-12 |
human |
| H L Kaung, C Y Wang, W W Che. Development of rat pancreatic islet cells in vitro. Microscopy research and technique. vol 43. issue 4. 1999-02-19. PMID:9849971. |
northern blot analyses also showed corresponding changes of insulin expression in these cells at different passages. |
1999-02-19 |
2023-08-12 |
rat |
| H L Kaung, C Y Wang, W W Che. Development of rat pancreatic islet cells in vitro. Microscopy research and technique. vol 43. issue 4. 1999-02-19. PMID:9849971. |
by passage 35, no insulin or glucagon expression was detectable by northern blot analysis or immunocytochemistry. |
1999-02-19 |
2023-08-12 |
rat |
| T A Kupriyanova, K V Kandro. Akt-2 binds to Glut4-containing vesicles and phosphorylates their component proteins in response to insulin. The Journal of biological chemistry. vol 274. issue 3. 1999-02-11. PMID:9880520. |
by means of monoq chromatography and western blot analysis, vesicle-associated protein kinase is identified as akt-2, a lipid-binding protein kinase involved in insulin signaling. |
1999-02-11 |
2023-08-12 |
rat |
| R Henningsson, P Alm, P Ekström, I Lundquis. Heme oxygenase and carbon monoxide: regulatory roles in islet hormone release: a biochemical, immunohistochemical, and confocal microscopic study. Diabetes. vol 48. issue 1. 1999-02-10. PMID:9892224. |
western blot showed that islet tissue expressed ho-2, and confocal microscopy revealed that ho-2 resided in insulin, glucagon, somatostatin, and pancreatic polypeptide cells. |
1999-02-10 |
2023-08-12 |
mouse |
| R Schechter, T Yanovitch, M Abboud, G Johnson, J Gaskin. Effects of brain endogenous insulin on neurofilament and MAPK in fetal rat neuron cell cultures. Brain research. vol 808. issue 2. 1998-12-11. PMID:9767173. |
western blot analysis showed that the basal level of mitogen activated protein kinase (mapk) phosphorylation was inhibited by the treatment of the ncc with isoproterenol or trypsin, but was significantly increased by treatment with exogenous insulin, demonstrating that brain endogenous insulin phosphorylated mapk (p<0.05). |
1998-12-11 |
2023-08-12 |
rat |
| D Lu, H Tamemoto, H Shibata, I Saito, T Takeuch. Regulatable production of insulin from primary-cultured hepatocytes: insulin production is up-regulated by glucagon and cAMP and down-regulated by insulin. Gene therapy. vol 5. issue 7. 1998-11-30. PMID:9813659. |
addition of exogenous insulin to the culture decreased insulin mrna expression remarkably on northern blot. |
1998-11-30 |
2023-08-12 |
Not clear |