| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| James A Rillema, Charles H Williams, Jerome Moulden, Kish L Golde. Effect of insulin on iodide uptake in mouse mammary gland explants. Experimental biology and medicine (Maywood, N.J.). vol 227. issue 1. 2002-01-30. PMID:11788781. |
the optimal cortisol concentration for a maximum stimulation of iodide uptake was 10(-7)m. in a quantitative western blot analysis employing an antibody to the sodium-iodide symporter, insulin stimulated an upregulation of the transporter protein after a 4-, 8-, or 20-hr treatment with insulin. |
2002-01-30 |
2023-08-12 |
mouse |
| V A Skeberdis, J Lan, X Zheng, R S Zukin, M V Bennet. Insulin promotes rapid delivery of N-methyl-D- aspartate receptors to the cell surface by exocytosis. Proceedings of the National Academy of Sciences of the United States of America. vol 98. issue 6. 2001-12-04. PMID:11248117. |
the present study shows that insulin induced (i) an increase in channel number times open probability (np(o)) in outside-out patches excised from xenopus oocytes, with no change in mean open time, unitary conductance, or reversal potential, indicating an increase in n and/or p(o); (ii) an increase in charge transfer during block of nmda-elicited currents by the open channel blocker mk-801, indicating increased number of functional nmdars in the cell membrane with no change in p(o); and (iii) increased nr1 surface expression, as indicated by western blot analysis of surface proteins. |
2001-12-04 |
2023-08-12 |
xenopus_laevis |
| A V Chibalin, M V Kovalenko, J W Ryder, E Féraille, H Wallberg-Henriksson, J R Zierat. Insulin- and glucose-induced phosphorylation of the Na(+),K(+)-adenosine triphosphatase alpha-subunits in rat skeletal muscle. Endocrinology. vol 142. issue 8. 2001-08-16. PMID:11459793. |
immunoprecipitation with antiphosphotyrosine antibody and subsequent western blot analysis with anti-alpha(1)- and -alpha(2)-subunit antibodies revealed that both alpha(1)- and alpha(2)-subunit isoforms underwent phosphorylation on tyrosine residues in response to insulin, although with different time course and magnitude. |
2001-08-16 |
2023-08-12 |
rat |
| X Han, B E Tuc. Cloning and characterization of porcine insulin gene. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. vol 129. issue 1. 2001-08-09. PMID:11337252. |
northern blot analysis of isolated adult porcine islets demonstrated an increase in steady-state insulin mrna levels in response to high concentrations of glucose. |
2001-08-09 |
2023-08-12 |
Not clear |
| N Chang, J M Uribe, S J Keely, S Calandrella, K E Barret. Insulin and IGF-I inhibit calcium-dependent chloride secretion by T84 human colonic epithelial cells. American journal of physiology. Gastrointestinal and liver physiology. vol 281. issue 1. 2001-07-19. PMID:11408264. |
western blot analysis showed that both insulin and igf-i recruited the 85-kda regulatory and 110-kda catalytic subunits of pi 3-kinase to anti-phosphotyrosine immunoprecipitates. |
2001-07-19 |
2023-08-12 |
human |
| S Morimoto, M A Cerbón, A Alvarez-Alvarez, G Romero-Navarro, V Díaz-Sánche. Insulin gene expression pattern in rat pancreas during the estrous cycle. Life sciences. vol 68. issue 26. 2001-07-05. PMID:11411797. |
a northern blot analysis was performed to assess insulin gene expression in pancreatic tissue. |
2001-07-05 |
2023-08-12 |
rat |
| R Hernandez, T Teruel, M Lorenz. Akt mediates insulin induction of glucose uptake and up-regulation of GLUT4 gene expression in brown adipocytes. FEBS letters. vol 494. issue 3. 2001-05-17. PMID:11311245. |
in consequence, ml-9 precluded insulin stimulation of glucose uptake and glut4 translocation to plasma membrane (determined by western blot), without any effect on the basal glucose uptake. |
2001-05-17 |
2023-08-12 |
rat |
| X Pan, S S Solomon, D M Borromeo, A Martinez-Hernandez, R Ragho. Insulin deprivation leads to deficiency of Sp1 transcription factor in H-411E hepatoma cells and in streptozotocin-induced diabetic ketoacidosis in the rat. Endocrinology. vol 142. issue 4. 2001-04-26. PMID:11250945. |
we demonstrated in rat liver tissue by both western blot and immunohistochemical staining with anti-sp1 antibody that 1) livers of fully diabetic streptozotocin rats have low levels of sp1 transcription factor; and 2) insulin treatment of the diabetic rat rapidly reversed this process by markedly stimulating accumulation of sp1 in rat liver. |
2001-04-26 |
2023-08-12 |
rat |
| G R Devi, D H Yang, R G Rosenfeld, Y O. Differential effects of insulin-like growth factor (IGF)-binding protein-3 and its proteolytic fragments on ligand binding, cell surface association, and IGF-I receptor signaling. Endocrinology. vol 141. issue 11. 2000-12-22. PMID:11089550. |
based on the ascertained and predicted recognition sites for known igfbp-3 proteases, flag-epitope tagged intact igfbp-3, nh2-terminal (1-97), intermediate fragment (88-148), and cooh-terminal fragments (98-264) and (184-264) were generated in a baculovirus and/or escherichia coli expression system and examined, by western ligand blot and affinity cross-linking assays, for their ability to bind igf and insulin. |
2000-12-22 |
2023-08-12 |
Not clear |
| T Utsugi, T Ohno, Y Ohyama, T Uchiyama, Y Saito, Y Matsumura, H Aizawa, H Itoh, M Kurabayashi, S Kawazu, S Tomono, Y Oka, T Suga, M Kuro-o, Y Nabeshima, R Naga. Decreased insulin production and increased insulin sensitivity in the klotho mutant mouse, a novel animal model for human aging. Metabolism: clinical and experimental. vol 49. issue 9. 2000-10-12. PMID:11016890. |
decreased insulin production was also supported by northern blot analysis showing lower levels of insulin mrna in kl/kl mice. |
2000-10-12 |
2023-08-12 |
mouse |
| K Handoko, K Yang, B Strutt, W Khalil, D Killinge. Insulin attenuates the stimulatory effects of tumor necrosis factor alpha on 11beta-hydroxysteroid dehydrogenase 1 in human adipose stromal cells. The Journal of steroid biochemistry and molecular biology. vol 72. issue 3-4. 2000-08-25. PMID:10775808. |
northern blot analysis revealed corresponding changes in the level of 11beta-hsd1 mrna, suggesting that the effects of tnfalpha and insulin on 11beta-hsd1 activity are mediated at the level of gene transcription. |
2000-08-25 |
2023-08-12 |
human |
| Y Ding, N D Vaziri, R Coulson, V S Kamanna, D D Ro. Effects of simulated hyperglycemia, insulin, and glucagon on endothelial nitric oxide synthase expression. American journal of physiology. Endocrinology and metabolism. vol 279. issue 1. 2000-08-08. PMID:10893317. |
the rates of basal and bradykinin-stimulated no production (nitrate + nitrite) and enos protein expression (western blot) were then determined at the basal condition and in the presence of insulin (10(-8) and 10(-7) m), glucagon (10(-8) and 10(-7) m), or both. |
2000-08-08 |
2023-08-12 |
human |
| S Jormsjö, S Ye, J Moritz, D H Walter, S Dimmeler, A M Zeiher, A Henney, A Hamsten, P Eriksso. Allele-specific regulation of matrix metalloproteinase-12 gene activity is associated with coronary artery luminal dimensions in diabetic patients with manifest coronary artery disease. Circulation research. vol 86. issue 9. 2000-06-14. PMID:10807873. |
furthermore, western blot analysis demonstrated that insulin increased mmp-12 protein production. |
2000-06-14 |
2023-08-12 |
Not clear |
| L Yau, H Lukes, H McDiarmid, J Werner, P Zahradk. Insulin-like growth factor-I (IGF-I)-dependent activation of pp42/44 mitogen-activated protein kinase occurs independently of IGF-I receptor kinase activation and IRS-1 tyrosine phosphorylation. European journal of biochemistry. vol 266. issue 3. 2000-01-31. PMID:10583412. |
western blot analysis demonstrated that tyrosine phosphorylation patterns produced by insulin and igf-i differed. |
2000-01-31 |
2023-08-12 |
Not clear |
| C Deachapunya, M Palmer-Densmore, S M O'Grad. Insulin stimulates transepithelial sodium transport by activation of a protein phosphatase that increases Na-K ATPase activity in endometrial epithelial cells. The Journal of general physiology. vol 114. issue 4. 1999-11-19. PMID:10498674. |
immunohistochemical localization along with western blot analysis of cultured porcine endometrial epithelial cells revealed the presence of alpha-1 and alpha-2 isoforms, but not the alpha-3 isoform of na(+)-k(+) atpase, which did not change in the presence of insulin. |
1999-11-19 |
2023-08-12 |
Not clear |
| T H Elsasser, S Kahl, A Martínez, L M Montuenga, R Pio, F Cuttitt. Adrenomedullin binding protein in the plasma of multiple species: characterization by radioligand blotting. Endocrinology. vol 140. issue 10. 1999-10-12. PMID:10499551. |
a series of peptide fragments of am representing amino- and carboxyterminal regions of the hormone, or amylin, calcitonin gene-related peptide (cgrp), or insulin failed to displace intact 125i-am from ligand blot bands. |
1999-10-12 |
2023-08-12 |
mouse |
| F Pacifico, D Liguoro, R Acquaviva, S Formisano, E Consigli. Thyroglobulin binding and TSH regulation of the RHL-1 subunit of the asialoglycoprotein receptor in rat thyroid. Biochimie. vol 81. issue 5. 1999-09-02. PMID:10403180. |
moreover, rt-pcr and western blot assays show that tsh deprivation decreases rhl-1 expression in pc c13 thyroid differentiated cells whereas insulin deprivation does not have any effect. |
1999-09-02 |
2023-08-12 |
rat |
| C Benes, V Poitout, J C Marie, J Martin-Perez, M P Roisin, R Fagar. Mode of regulation of the extracellular signal-regulated kinases in the pancreatic beta-cell line MIN6 and their implication in the regulation of insulin gene transcription. The Biochemical journal. vol 340 ( Pt 1). 1999-06-30. PMID:10229678. |
this was observed by analysis of the transfected rat insulin i gene promoter activity and a northern blot of endogenous insulin mrna. |
1999-06-30 |
2023-08-12 |
rat |
| J E Friedman, T Ishizuka, S Liu, C J Farrell, R J Koletsky, D Bedol, P Ernsberge. Anti-hyperglycemic activity of moxonidine: metabolic and molecular effects in obese spontaneously hypertensive rats. Blood pressure. Supplement. vol 3. 1999-06-24. PMID:10321453. |
western blot analysis of insulin signaling proteins showed that irs-1 was decreased 42% in control shrob compared with shr. |
1999-06-24 |
2023-08-12 |
human |
| E Sciullo, G Cardellini, M G Baroni, P Torresi, A Buongiorno, P Pozzilli, F Fallucc. Glucose transporter (Glut1, Glut3) mRNA in human placenta of diabetic and non-diabetic pregnancies. Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy. vol 3. issue 3. 1999-04-15. PMID:10086067. |
we therefore investigated placental glut1 and glut3 mrna by northern blot analysis in ten diabetic (five insulin dependent diabetes mellitus (iddm), two non-insulin dependent diabetes mellitus (niddm) and three gestational diabetes mellitus (gdm)) and nine non-diabetic women. |
1999-04-15 |
2023-08-12 |
human |