| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Y S Guo, C M Townsend, G F Jin, R D Beauchamp, J C Thompso. Differential regulation by TGF-beta 1 and insulin of insulin-like growth factor binding protein-2 in IEC-6 cells. The American journal of physiology. vol 268. issue 6 Pt 1. 1995-08-15. PMID:7541944. |
utilizing western ligand blot analysis, we found that tgf-beta 1 at concentrations of 0.5, 1.0, and 2 ng/ml significantly increased levels of 32-kda igfbp in the conditioned medium (cm) of iec-6 cells in a dose-dependent fashion and that low doses of insulin (1.0 and 5.0 microgram/ml) also increased igfbp levels in the cm of iec-6 cells, but a high dose of insulin (10 micrograms/ml) depressed igfbp release in the cm. |
1995-08-15 |
2023-08-12 |
Not clear |
| N Welsh, K Bendtzen, M Wels. Expression of an insulin/interleukin-1 receptor antagonist hybrid gene in insulin-producing cell lines (HIT-T15 and NIT-1) confers resistance against interleukin-1-induced nitric oxide production. The Journal of clinical investigation. vol 95. issue 4. 1995-05-09. PMID:7706480. |
one of the geneticin-selected clones, hitra2, expressed a 1.4-kb mrna, which hybridized both to insulin and il-1ra-cdna in northern blot analysis. |
1995-05-09 |
2023-08-12 |
human |
| P J Miettinen, T Otonkoski, R Voutilaine. Insulin-like growth factor-II and transforming growth factor-alpha in developing human fetal pancreatic islets. The Journal of endocrinology. vol 138. issue 1. 1995-03-10. PMID:7852883. |
northern blot analysis revealed an abundant expression of igf-ii, insulin and tgf-alpha mrnas in the intact pancreas and the cultured iccs. |
1995-03-10 |
2023-08-12 |
human |
| I Conget, Y Sarri, A Novials, R Casamitjana, M Vives, R Gomi. Functional properties of isolated human pancreatic islets beneficial effects of culture and exposure to high glucose concentrations. Diabete & metabolisme. vol 20. issue 2. 1995-02-02. PMID:7805958. |
northern blot analysis was performed to measure insulin mrna levels. |
1995-02-02 |
2023-08-12 |
human |
| B Tang, S Guller, E Gurpid. Mechanisms involved in the decidualization of human endometrial stromal cells. Acta Europaea fertilitatis. vol 24. issue 5. 1995-01-05. PMID:7985467. |
exposure of stromal cell cultures to db-camp, 8-br-camp or forskolin in rpmi 1640 medium containing 2% ct-fcs and 0.1 u/ml insulin induces the expression of prolactin (prl), evident from 1) its secretion to the culture medium, measured by radioimmunoassay and by western blot analysis, 2) the incorporation of 35s-methionine in a protein precipitated with a prl antibody and co-migrating with authentic prl during electrophoresis, and 3) the synthesis of prl mrna determined by northern blot analysis. |
1995-01-05 |
2023-08-12 |
human |
| F S Celi, K Tanner, A K Roth, A E Roth, A R Shuldine. The two nonallelic Xenopus insulin genes are expressed coordinately in the adult pancreas. General and comparative endocrinology. vol 95. issue 2. 1994-12-28. PMID:7525401. |
insulin i and ii mrnas were quantitated by slot blot hybridization with specific probes and were expressed as the number of copies (x 10(8)) per 5 micrograms total rna +/- sem. |
1994-12-28 |
2023-08-12 |
xenopus_laevis |
| E Oetjen, T Diedrich, A Eggers, B Eckert, W Knepe. Distinct properties of the cAMP-responsive element of the rat insulin I gene. The Journal of biological chemistry. vol 269. issue 43. 1994-11-23. PMID:7929445. |
in an electrophoretic mobility shift assay and in southwestern blot experiments, bacterially expressed recombinant creb bound to the insulin cre as it did to the rat glucagon and rat somatostatin gene cres. |
1994-11-23 |
2023-08-12 |
rat |
| D Montarras, C Pinset, M C Pérez, J Ilan, F Gro. Muscle differentiation: insulin-like growth factors as positive modulators of myogenic regulatory genes? Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. vol 316. issue 9. 1994-10-06. PMID:8076203. |
our results show that: (1) accumulation of igfii mrna is dramatically decreased (this mrna is undetectable by rna blotting); (2) accumulation of myod mrna also becomes undetectable by rna blot analysis; (3) the ability of the cells to differentiate, including the activation of myogenin and myosin genes, is severely compromised; (4) expression of the myod genes and the ability to differentiate are restored following the addition of insulin or igf to the cells. |
1994-10-06 |
2023-08-12 |
mouse |
| A Zarain-Herzberg, K Yano, V Elimban, N S Dhall. Cardiac sarcoplasmic reticulum Ca(2+)-ATPase expression in streptozotocin-induced diabetic rat heart. Biochemical and biophysical research communications. vol 203. issue 1. 1994-09-29. PMID:8074644. |
northern blot and slot blot analyses did not show statistically significant reduction in the relative level of serca2 mrna expression in diabetic or insulin treated rats. |
1994-09-29 |
2023-08-12 |
rat |
| A Zarain-Herzberg, K Yano, V Elimban, N S Dhall. Cardiac sarcoplasmic reticulum Ca(2+)-ATPase expression in streptozotocin-induced diabetic rat heart. Biochemical and biophysical research communications. vol 203. issue 1. 1994-09-29. PMID:8074644. |
quantitation of serca2 protein by western blot did not reveal any change in diabetic and insulin treated animals. |
1994-09-29 |
2023-08-12 |
rat |
| I M el-Kebbi, S Roser, R J Polle. Regulation of glucose transport by pioglitazone in cultured muscle cells. Metabolism: clinical and experimental. vol 43. issue 8. 1994-09-08. PMID:8052151. |
northern and western blot studies on glucose-treated cells (25 mmol/l) showed that glucose transporter mrna and protein increased in parallel following treatment with either pioglitazone or insulin alone. |
1994-09-08 |
2023-08-12 |
Not clear |
| F E Thorngate, R Raghow, H G Wilcox, C S Werner, M Heimberg, M B Ela. Insulin promotes the biosynthesis and secretion of apolipoprotein B-48 by altering apolipoprotein B mRNA editing. Proceedings of the National Academy of Sciences of the United States of America. vol 91. issue 12. 1994-07-06. PMID:8202496. |
neither insulin nor oleic acid affects total apob mrna levels as assayed by northern blot analysis. |
1994-07-06 |
2023-08-12 |
rat |
| A J Lange, D Argaud, M R el-Maghrabi, W Pan, S R Maitra, S J Pilki. Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP. Biochemical and biophysical research communications. vol 201. issue 1. 1994-06-29. PMID:8198588. |
northern blot analysis of rna from fao hepatoma cells revealed that dexamethasone induced the glucose-6-phosphatase mrna while insulin suppressed its expression. |
1994-06-29 |
2023-08-12 |
mouse |
| S Feilleux-Duché, M Garlatti, R Burcelin, M Aggerbeck, J Bouguet, J Girard, J Hanoune, R Barouk. Acinar zonation of the hormonal regulation of cytosolic aspartate aminotransferase in the liver. The American journal of physiology. vol 266. issue 4 Pt 1. 1994-06-09. PMID:7513955. |
northern blot analysis showed that the caspat mrnas were increased by those treatments; the effect of streptozotocin was reversed by the administration of insulin. |
1994-06-09 |
2023-08-12 |
rat |
| T Ohnishi, O Nakamura, N Arakaki, H Miyazaki, Y Daikuhar. Effects of cytokines and growth factors on phosphorylated fetuin biosynthesis by adult rat hepatocytes in primary culture. Biochemical and biophysical research communications. vol 200. issue 1. 1994-05-23. PMID:7513166. |
here we report that the rate of synthesis of phosphofetuin by hepatocytes in culture was reduced by inflammatory cytokines such as human interleukin (hil)-6, human tumor necrosis factor-alpha and hil-1 alpha, but dose-dependently stimulated by growth factors of hepatocytes, such as hepatocyte growth factor (hgf)/scatter factor (sf), epidermal growth factor and insulin, as determined by metabolic labeling and northern blot analysis using cdna for rat fetuin as a probe. |
1994-05-23 |
2023-08-12 |
human |
| L A Sechi, C A Griffin, M Schambela. Effect of dietary sodium chloride on insulin receptor number and mRNA levels in rat kidney. The American journal of physiology. vol 266. issue 1 Pt 2. 1994-03-09. PMID:8304482. |
insulin receptor mrna, as quantified by northern and slot blot analysis, was inversely related to salt intake in absence of a change in plasma glucose, insulin, and corticosterone levels. |
1994-03-09 |
2023-08-12 |
rat |
| J Hogg, V K Han, D R Clemmons, D J Hil. Interactions of nutrients, insulin-like growth factors (IGFs) and IGF-binding proteins in the regulation of DNA synthesis by isolated fetal rat islets of Langerhans. The Journal of endocrinology. vol 138. issue 3. 1994-02-10. PMID:7506287. |
the release of insulin and endogenous igf-i and -ii were each determined by radioimmunoassay, and igfbp release characterized by western ligand blot analysis. |
1994-02-10 |
2023-08-12 |
rat |
| T Matsuda, Y Murata, N Kawamura, M Hayashi, K Tamada, K Takuma, S Maeda, A Bab. Selective induction of alpha 1 isoform of (Na+ + K+)-ATPase by insulin/insulin-like growth factor-I in cultured rat astrocytes. Archives of biochemistry and biophysics. vol 307. issue 1. 1993-12-21. PMID:8239654. |
western blot analysis showed that alpha 1 and alpha 2 isoforms of (na+ + k+)-atpase were present in cultured astrocytes and that insulin and igf-i increased the content of the alpha 1 isoform but did not that of the alpha 2 isoform. |
1993-12-21 |
2023-08-12 |
rat |
| D J Gwilliam, P M Jones, S J Persaud, S L Howel. The role of protein kinase C in insulin biosynthesis. Acta diabetologica. vol 30. issue 2. 1993-12-06. PMID:8219266. |
these elevations in the basal rate of insulin synthesis were not secondary to an increase in the amount of preproinsulin mrna in pkc-depleted islets since northern blot analysis showed that prolonged exposure to 4 beta-pma, and the subsequent loss of pkc activity, did not detectably alter basal levels of preproinsulin mrna. |
1993-12-06 |
2023-08-12 |
rat |
| F Dandoy-Dron, L Deltour, E Monthioux, D Bucchini, J Jam. Insulin gene can be expressed in the absence of Isl-1. Experimental cell research. vol 209. issue 1. 1993-11-26. PMID:8224007. |
the possible role of isl-1 in insulin-gene transcription was investigated using northern blot analysis to determine whether insulin and isl-1 gene expression are correlated in various somatic cell hybrids. |
1993-11-26 |
2023-08-12 |
mouse |