| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| E Oetjen, T Diedrich, A Eggers, B Eckert, W Knepe. Distinct properties of the cAMP-responsive element of the rat insulin I gene. The Journal of biological chemistry. vol 269. issue 43. 1994-11-23. PMID:7929445. |
in an electrophoretic mobility shift assay and in southwestern blot experiments, bacterially expressed recombinant creb bound to the insulin cre as it did to the rat glucagon and rat somatostatin gene cres. |
1994-11-23 |
2023-08-12 |
rat |
| D Montarras, C Pinset, M C Pérez, J Ilan, F Gro. Muscle differentiation: insulin-like growth factors as positive modulators of myogenic regulatory genes? Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. vol 316. issue 9. 1994-10-06. PMID:8076203. |
our results show that: (1) accumulation of igfii mrna is dramatically decreased (this mrna is undetectable by rna blotting); (2) accumulation of myod mrna also becomes undetectable by rna blot analysis; (3) the ability of the cells to differentiate, including the activation of myogenin and myosin genes, is severely compromised; (4) expression of the myod genes and the ability to differentiate are restored following the addition of insulin or igf to the cells. |
1994-10-06 |
2023-08-12 |
mouse |
| A Zarain-Herzberg, K Yano, V Elimban, N S Dhall. Cardiac sarcoplasmic reticulum Ca(2+)-ATPase expression in streptozotocin-induced diabetic rat heart. Biochemical and biophysical research communications. vol 203. issue 1. 1994-09-29. PMID:8074644. |
northern blot and slot blot analyses did not show statistically significant reduction in the relative level of serca2 mrna expression in diabetic or insulin treated rats. |
1994-09-29 |
2023-08-12 |
rat |
| A Zarain-Herzberg, K Yano, V Elimban, N S Dhall. Cardiac sarcoplasmic reticulum Ca(2+)-ATPase expression in streptozotocin-induced diabetic rat heart. Biochemical and biophysical research communications. vol 203. issue 1. 1994-09-29. PMID:8074644. |
quantitation of serca2 protein by western blot did not reveal any change in diabetic and insulin treated animals. |
1994-09-29 |
2023-08-12 |
rat |
| I M el-Kebbi, S Roser, R J Polle. Regulation of glucose transport by pioglitazone in cultured muscle cells. Metabolism: clinical and experimental. vol 43. issue 8. 1994-09-08. PMID:8052151. |
northern and western blot studies on glucose-treated cells (25 mmol/l) showed that glucose transporter mrna and protein increased in parallel following treatment with either pioglitazone or insulin alone. |
1994-09-08 |
2023-08-12 |
Not clear |
| F E Thorngate, R Raghow, H G Wilcox, C S Werner, M Heimberg, M B Ela. Insulin promotes the biosynthesis and secretion of apolipoprotein B-48 by altering apolipoprotein B mRNA editing. Proceedings of the National Academy of Sciences of the United States of America. vol 91. issue 12. 1994-07-06. PMID:8202496. |
neither insulin nor oleic acid affects total apob mrna levels as assayed by northern blot analysis. |
1994-07-06 |
2023-08-12 |
rat |
| A J Lange, D Argaud, M R el-Maghrabi, W Pan, S R Maitra, S J Pilki. Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP. Biochemical and biophysical research communications. vol 201. issue 1. 1994-06-29. PMID:8198588. |
northern blot analysis of rna from fao hepatoma cells revealed that dexamethasone induced the glucose-6-phosphatase mrna while insulin suppressed its expression. |
1994-06-29 |
2023-08-12 |
mouse |
| S Feilleux-Duché, M Garlatti, R Burcelin, M Aggerbeck, J Bouguet, J Girard, J Hanoune, R Barouk. Acinar zonation of the hormonal regulation of cytosolic aspartate aminotransferase in the liver. The American journal of physiology. vol 266. issue 4 Pt 1. 1994-06-09. PMID:7513955. |
northern blot analysis showed that the caspat mrnas were increased by those treatments; the effect of streptozotocin was reversed by the administration of insulin. |
1994-06-09 |
2023-08-12 |
rat |
| T Ohnishi, O Nakamura, N Arakaki, H Miyazaki, Y Daikuhar. Effects of cytokines and growth factors on phosphorylated fetuin biosynthesis by adult rat hepatocytes in primary culture. Biochemical and biophysical research communications. vol 200. issue 1. 1994-05-23. PMID:7513166. |
here we report that the rate of synthesis of phosphofetuin by hepatocytes in culture was reduced by inflammatory cytokines such as human interleukin (hil)-6, human tumor necrosis factor-alpha and hil-1 alpha, but dose-dependently stimulated by growth factors of hepatocytes, such as hepatocyte growth factor (hgf)/scatter factor (sf), epidermal growth factor and insulin, as determined by metabolic labeling and northern blot analysis using cdna for rat fetuin as a probe. |
1994-05-23 |
2023-08-12 |
human |
| L A Sechi, C A Griffin, M Schambela. Effect of dietary sodium chloride on insulin receptor number and mRNA levels in rat kidney. The American journal of physiology. vol 266. issue 1 Pt 2. 1994-03-09. PMID:8304482. |
insulin receptor mrna, as quantified by northern and slot blot analysis, was inversely related to salt intake in absence of a change in plasma glucose, insulin, and corticosterone levels. |
1994-03-09 |
2023-08-12 |
rat |
| J Hogg, V K Han, D R Clemmons, D J Hil. Interactions of nutrients, insulin-like growth factors (IGFs) and IGF-binding proteins in the regulation of DNA synthesis by isolated fetal rat islets of Langerhans. The Journal of endocrinology. vol 138. issue 3. 1994-02-10. PMID:7506287. |
the release of insulin and endogenous igf-i and -ii were each determined by radioimmunoassay, and igfbp release characterized by western ligand blot analysis. |
1994-02-10 |
2023-08-12 |
rat |
| T Matsuda, Y Murata, N Kawamura, M Hayashi, K Tamada, K Takuma, S Maeda, A Bab. Selective induction of alpha 1 isoform of (Na+ + K+)-ATPase by insulin/insulin-like growth factor-I in cultured rat astrocytes. Archives of biochemistry and biophysics. vol 307. issue 1. 1993-12-21. PMID:8239654. |
western blot analysis showed that alpha 1 and alpha 2 isoforms of (na+ + k+)-atpase were present in cultured astrocytes and that insulin and igf-i increased the content of the alpha 1 isoform but did not that of the alpha 2 isoform. |
1993-12-21 |
2023-08-12 |
rat |
| D J Gwilliam, P M Jones, S J Persaud, S L Howel. The role of protein kinase C in insulin biosynthesis. Acta diabetologica. vol 30. issue 2. 1993-12-06. PMID:8219266. |
these elevations in the basal rate of insulin synthesis were not secondary to an increase in the amount of preproinsulin mrna in pkc-depleted islets since northern blot analysis showed that prolonged exposure to 4 beta-pma, and the subsequent loss of pkc activity, did not detectably alter basal levels of preproinsulin mrna. |
1993-12-06 |
2023-08-12 |
rat |
| F Dandoy-Dron, L Deltour, E Monthioux, D Bucchini, J Jam. Insulin gene can be expressed in the absence of Isl-1. Experimental cell research. vol 209. issue 1. 1993-11-26. PMID:8224007. |
the possible role of isl-1 in insulin-gene transcription was investigated using northern blot analysis to determine whether insulin and isl-1 gene expression are correlated in various somatic cell hybrids. |
1993-11-26 |
2023-08-12 |
mouse |
| S L Bradshaw, V K Ha. Hormonal regulation of astroglial insulin-like growth factor (IGF)-binding protein gene expression by IGFs and insulin. Endocrinology. vol 133. issue 4. 1993-11-03. PMID:7691580. |
conditioned media from astroglial cultures without (control) or with hormonal treatment (10, 100, and 200 ng/ml igf-i or igf-ii and 0.1, 1.0, and 10 micrograms/ml insulin) were collected and subjected to western ligand blot analysis. |
1993-11-03 |
2023-08-12 |
rat |
| A Novials, Y Sarri, R Casamitjana, F Rivera, R Gomi. Regulation of islet amyloid polypeptide in human pancreatic islets. Diabetes. vol 42. issue 10. 1993-10-21. PMID:8375592. |
northern blot analysis of each cultured condition showed an increase of both mrna iapp and insulin signals after exposure of islets at 16.7 mm glucose, the maximum mrna expression being after long exposure to high-glucose concentrations. |
1993-10-21 |
2023-08-12 |
human |
| B Schnetzler, G Murakawa, D Abalos, P Halban, R Selde. Adaptation to supraphysiologic levels of insulin gene expression in transgenic mice: evidence for the importance of posttranscriptional regulation. The Journal of clinical investigation. vol 92. issue 1. 1993-08-12. PMID:8325994. |
northern blot and primer extension analyses indicated that more than twice as much insulin mrna is present in pancreata from transgenic mice. |
1993-08-12 |
2023-08-12 |
mouse |
| Y Oh, H L Müller, H Pham, G Lamson, R G Rosenfel. Insulin-like growth factor binding protein (IGFBP)-3 levels in conditioned media of Hs578T human breast cancer cells are post-transcriptionally regulated. Growth regulation. vol 3. issue 1. 1993-06-09. PMID:7683543. |
in this study, we have demonstrated that regulation of hs578t igfbp-3 by igf peptides is specific, non-receptor mediated, and post-translational by showing: 1) dose-dependent increase of igfbp-3 in conditioned media(cm) following addition of igf-i and igf-ii (maximum 8-13-fold increase at 100 ng/ml concentration), but not by insulin up to 1 microgram/ml; 2) confirmation of igf-induced increases in cm concentrations of igfbp-3 by means of western ligand blot, affinity cross-linking, and igfbp-3-specific radioimmunoassay; 3) increase of igfbp-3 in cm by addition of igf analogs which retain full affinity for igfbps ([leu27]igf-ii and [tyr55,gln56]igf-i), but not by igf analogs which have significantly decreased affinity for igfbps ([gln3,ala4,tyr15,leu16]igf-i, [gln6,ala7,tyr18,leu19,leu27]igf-ii and igf-i/insulin hybrid); 4) no change in igfbp-3 mrna following addition of igfs; 5) existence of metal ion-dependent igfbp-3 specific protease in cm and protection of igfbp-3 from protease by formation of [igf:igfbp-3] complexes; and 6) release of cell surface-associated igfbp-3 into cm by addition of igf peptides. |
1993-06-09 |
2023-08-12 |
human |
| J A Flores, J C Garmey, J E Nestler, J D Veldhui. Sites of inhibition of steroidogenesis by activation of protein kinase-C in swine ovarian (granulosa) cells. Endocrinology. vol 132. issue 5. 1993-05-25. PMID:8477649. |
cellular concentrations of p450scc mrna, as measured by northern blot hybridization with a 32p-labeled 1-kilobase porcine cdna clone, were significantly increased by insulin. |
1993-05-25 |
2023-08-12 |
Not clear |
| S U Devaskar, H F Sadiq, L Holtzclaw, M Georg. The developmental pattern of rabbit brain insulin and insulin-like growth factor receptor expression. Brain research. vol 605. issue 1. 1993-05-10. PMID:8467380. |
to examine the effect of development on rabbit brain insulin and insulin-like growth factor (igf) receptor expression, we characterized and quantitated receptor mrnas by northern blot analysis and affinity-labeled ligand bound receptors by sds-page and autoradiography. |
1993-05-10 |
2023-08-12 |
rabbit |