| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Y Fujii, K Hasumi, H Nakamura, Y Takahama, H Shimizu, I Sugawara, M Eriguchi, M Sekiguch. Characterization of a murine monoclonal antibody, 5D-4, reacting with pancreatic cancers and islets of Langerhans. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 46. issue 9. 1993-04-07. PMID:1292652. |
the 5d-4 mab reacted not only with ca 50 kda and 30 kda solubilized protein from aspc-1 cells by western blot analysis but also with human insulin in a competition ria. |
1993-04-07 |
2023-08-11 |
mouse |
| M C Hammond-Kosack, M W Kilpatrick, K Dochert. Analysis of DNA structure in the human insulin gene-linked polymorphic region in vivo. Journal of molecular endocrinology. vol 9. issue 3. 1993-02-05. PMID:1476609. |
following digestion with taqi and subsequent digestion with s1-nuclease to cleave at the bromoacetaldehyde-reactive sites, the dna was subjected to agarose gel electrophoresis, and insulin gene fragments were detected by southern blot analysis. |
1993-02-05 |
2023-08-11 |
human |
| N Sun, Z Su. [Effects of insulin on expression of oncogenes in CWE mouse brain]. Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao. vol 23. issue 2. 1993-01-07. PMID:1452149. |
by rna dot blot hybridization, we observed the expression of c-myc, c-fos, ha-ras and erbb genes in normal brain of cwe mouse after the injection of insulin. |
1993-01-07 |
2023-08-11 |
mouse |
| J Fowlkes, M Freemar. Evidence for a novel insulin-like growth factor (IGF)-dependent protease regulating IGF-binding protein-4 in dermal fibroblasts. Endocrinology. vol 131. issue 5. 1992-12-01. PMID:1385096. |
to determine whether the effects of igfs on igfbp-4 might be exerted through alterations in igfbp-4 degradation, we incubated cell-free, fibroblast-conditioned media from either sheep or human dermal fibroblasts with or without igf-i, igf-ii (each 1 microgram/ml), or insulin (10 micrograms/ml) for 72 h at 37 c. samples were then analyzed by western ligand blot using radiolabeled igfs and by immunoblotting using a polyclonal antisera to human igfbp-4. |
1992-12-01 |
2023-08-11 |
human |
| A Kanatsuka, H Makino, T Yamaguchi, H Ohsawa, Y Tokuyama, T Saitoh, K Yamamura, J Miyazaki, S Yoshid. Islet amyloid polypeptide/amylin in pancreatic beta-cell line derived from transgenic mouse insulinoma. Diabetes. vol 41. issue 11. 1992-11-19. PMID:1383068. |
rna blot analysis revealed an abundance of iapp and insulin ii mrna in the cells, findings comparable with those in the pancreas of a normal mouse. |
1992-11-19 |
2023-08-11 |
mouse |
| Y J Lin, S Harada, E G Loten, R M Smith, L Jaret. Direct stimulation of immediate-early genes by intranuclear insulin in trypsin-treated H35 hepatoma cells. Proceedings of the National Academy of Sciences of the United States of America. vol 89. issue 20. 1992-11-17. PMID:1409684. |
northern blot analysis revealed insulin increased g33 and c-fos mrna concentrations identically in control and trypsin-treated cells but had no effect on beta 2-microglobulin mrna. |
1992-11-17 |
2023-08-11 |
Not clear |
| R Yuasa, M C Eggo, J Meinkoth, W H Dillmann, G N Burro. Iodide induces transforming growth factor beta 1 (TGF-beta 1) mRNA in sheep thyroid cells. Thyroid : official journal of the American Thyroid Association. vol 2. issue 2. 1992-10-16. PMID:1525582. |
thyroid cells were incubated with tsh and five additives (insulin, somatostatin, growth hormone, transferrin, and glycyl-l-histidyl-l-lysin) for 2-3 weeks and then were exposed to sodium iodide (nai) or 1-methylimidazole-2-thiol (methimazole, mmi), or both for 72 h. iodide at 10(-6) m and 10(-4) m significantly increased the amount of tgf-beta mrna as determined by northern blot analysis with a rat tgf-beta 1 cdna probe. |
1992-10-16 |
2023-08-11 |
rat |
| M A Leal, C Cabañas, C Rius, P Aller, C Call. Modulation by dexamethasone of insulin binding and insulin receptor mRNA levels in U-937 human promonocytic cells. Biochimie. vol 74. issue 6. 1992-10-14. PMID:1520733. |
northern blot assays revealed two insulin receptor-related mrnas of approximately 11 and 7 kb in size. |
1992-10-14 |
2023-08-11 |
human |
| G W Gould, A M Brant, B B Kahn, P R Shepherd, S C McCoid, E M Gibb. Expression of the brain-type glucose transporter is restricted to brain and neuronal cells in mice. Diabetologia. vol 35. issue 4. 1992-10-06. PMID:1516757. |
northern blot analysis of human tissues has demonstrated the expression of the brain-type glucose transporter isoform (glut 3) in liver, muscle and fat, raising the possibility that this transporter isoform may play a role in the regulation of glucose disposal in these tissues in response to insulin. |
1992-10-06 |
2023-08-11 |
mouse |
| R Schechter, J Whitmire, L Holtzclaw, M George, R Harlow, S U Devaska. Developmental regulation of insulin in the mammalian central nervous system. Brain research. vol 582. issue 1. 1992-09-14. PMID:1482442. |
employing porcine/bovine or rabbit insulin as standards, we observed that brain insulin mimicked authentic insulin in its migration on sds-polyacrylamide and native gel electrophoresis, immunogenicity on western blot analysis, and its elution profile on immunoaffinity column chromatographic, and high performance liquid chromatographic separation. |
1992-09-14 |
2023-08-11 |
rabbit |
| R Schechter, J Whitmire, L Holtzclaw, M George, R Harlow, S U Devaska. Developmental regulation of insulin in the mammalian central nervous system. Brain research. vol 582. issue 1. 1992-09-14. PMID:1482442. |
a search for insulin mrna by northern blot analysis yielded minute amounts of atypical large sized transcripts. |
1992-09-14 |
2023-08-11 |
rabbit |
| S A Dekowski, J M Snyde. Insulin regulation of messenger ribonucleic acid for the surfactant-associated proteins in human fetal lung in vitro. Endocrinology. vol 131. issue 2. 1992-09-02. PMID:1639013. |
in the present study, we used northern blot analysis to evaluate the effects of insulin on the content of sp-a messenger rna (mrna) as well as on the content of mrna for the hydrophobic surfactant-associated proteins sp-b and sp-c in human fetal lung explants maintained in vitro. |
1992-09-02 |
2023-08-11 |
human |
| B Thorens, Y J Wu, J L Leahy, G C Wei. The loss of GLUT2 expression by glucose-unresponsive beta cells of db/db mice is reversible and is induced by the diabetic environment. The Journal of clinical investigation. vol 90. issue 1. 1992-08-24. PMID:1634622. |
glucose-induced insulin secretion by beta cells of diabetic db/db mice was studied by a pancreas perfusion technique, and the levels of glut2 protein in pancreatic islets were assessed by immunofluorescence microscopy and protein blot analysis. |
1992-08-24 |
2023-08-11 |
mouse |
| B Bhandari, K S Saini, R E Mille. Glycerol 3-phosphate dehydrogenase gene expression in cultured 3T3-L1 adipocytes: regulation by insulin, dexamethasone and dibutyryl cAMP at the level of mRNA abundance, transcription and mRNA stability. Molecular and cellular endocrinology. vol 76. issue 1-3. 1992-08-11. PMID:1668204. |
an increase by insulin (4-fold) and decrease by dexamethasone (by 50%) and dibutyryl camp (by 70%) was observed for g3pdh mrna abundance as analyzed by northern blot hybridization. |
1992-08-11 |
2023-08-11 |
human |
| P J Miettinen, K Heikinheim. Transforming growth factor-alpha (TGF-alpha) and insulin gene expression in human fetal pancreas. Development (Cambridge, England). vol 114. issue 4. 1992-07-31. PMID:1618147. |
in northern blot analysis a single band of 4.8 kilobases (kb) corresponding to the tgf-alpha transcript and a 0.6 kb for the insulin mrna were detected in the pancreas. |
1992-07-31 |
2023-08-11 |
human |
| M E Thompson, W E Zimmer, A L Haynes, D L Valentine, S Forss-Petter, J G Scammel. Prolactin granulogenesis is associated with increased secretogranin expression and aggregation in the Golgi apparatus of GH4C1 cells. Endocrinology. vol 131. issue 1. 1992-07-27. PMID:1612012. |
gh cells were treated for up to 3 days with 17 beta-estradiol (1 nm), insulin (300 nm), and epidermal growth factor (10 nm) and were fixed in 4% paraformaldehyde for immunocytochemistry or harvested for rna isolation and northern blot analysis. |
1992-07-27 |
2023-08-11 |
Not clear |
| P A Farrell, A L Caston, D Rodd, J Engdah. Effect of training on insulin secretion from single pancreatic beta cells. Medicine and science in sports and exercise. vol 24. issue 4. 1992-05-14. PMID:1560738. |
in vitro insulin secretion from single beta cells was measured using the cell blot assay (kendall and hymer, endocrinology, 121:2260-2262, 1987) and t, had lower secretion 2.6 +/- 1.4 pg.cell-1 when compared (p less than 0.05) with s, 6.7 +/- 0.6, but not lower than pf, 4.1 +/- 1.7 pg.cell-1. |
1992-05-14 |
2023-08-11 |
rat |
| S P Campos, H Bauman. Insulin is a prominent modulator of the cytokine-stimulated expression of acute-phase plasma protein genes. Molecular and cellular biology. vol 12. issue 4. 1992-04-22. PMID:1372389. |
quantitation of c/ebp beta mrna by northern (rna) blot analysis and of c/ebp beta dna binding activity by southwestern (dna-protein) blot analysis showed that insulin, when combined with cytokines and dexamethasone, stimulated both the mrna and dna binding activity by a factor of 1.6 compared with that of cells treated with cytokines and dexamethasone alone. |
1992-04-22 |
2023-08-11 |
human |
| S Nagata, K Inoue, T Sensh. Estrogen-insulin synergism in induction of prolactin in growth hormone-producing cells. Molecular and cellular endocrinology. vol 82. issue 2-3. 1992-04-07. PMID:1794611. |
incorporation of [35s]amino acids into immunoprecipitable prl became detectable at 15 h of culture with insulin (500 ng/ml) and increased up to 48 h. northern blot hybridization demonstrated a concurrent induction of prl mrna. |
1992-04-07 |
2023-08-11 |
Not clear |
| F J Oliver, G de la Rubia, E P Feener, M E Lee, M R Loeken, T Shiba, T Quertermous, G L Kin. Stimulation of endothelin-1 gene expression by insulin in endothelial cells. The Journal of biological chemistry. vol 266. issue 34. 1992-01-13. PMID:1744120. |
by rna blot analysis, insulin (1.67 x 10(-8) m) increased et-1 mrna levels by 2.3-fold over the basal within 10 min and attained a maximum (5.3-fold increase) in 2 h. dose-response studies showed that a maximum effect of insulin was reached at 1.67 x 10(-8) m although a significant increase can be observed at 1.66 x 10(-9) m. radioligand receptor studies indicated that the affinity constant for insulin receptors on endothelial cells correlated closely with the dose response observed for et-1 mrna. |
1992-01-13 |
2023-08-11 |
cat |