| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| S L Bradshaw, V K Ha. Hormonal regulation of astroglial insulin-like growth factor (IGF)-binding protein gene expression by IGFs and insulin. Endocrinology. vol 133. issue 4. 1993-11-03. PMID:7691580. |
conditioned media from astroglial cultures without (control) or with hormonal treatment (10, 100, and 200 ng/ml igf-i or igf-ii and 0.1, 1.0, and 10 micrograms/ml insulin) were collected and subjected to western ligand blot analysis. |
1993-11-03 |
2023-08-12 |
rat |
| A Novials, Y Sarri, R Casamitjana, F Rivera, R Gomi. Regulation of islet amyloid polypeptide in human pancreatic islets. Diabetes. vol 42. issue 10. 1993-10-21. PMID:8375592. |
northern blot analysis of each cultured condition showed an increase of both mrna iapp and insulin signals after exposure of islets at 16.7 mm glucose, the maximum mrna expression being after long exposure to high-glucose concentrations. |
1993-10-21 |
2023-08-12 |
human |
| B Schnetzler, G Murakawa, D Abalos, P Halban, R Selde. Adaptation to supraphysiologic levels of insulin gene expression in transgenic mice: evidence for the importance of posttranscriptional regulation. The Journal of clinical investigation. vol 92. issue 1. 1993-08-12. PMID:8325994. |
northern blot and primer extension analyses indicated that more than twice as much insulin mrna is present in pancreata from transgenic mice. |
1993-08-12 |
2023-08-12 |
mouse |
| Y Oh, H L Müller, H Pham, G Lamson, R G Rosenfel. Insulin-like growth factor binding protein (IGFBP)-3 levels in conditioned media of Hs578T human breast cancer cells are post-transcriptionally regulated. Growth regulation. vol 3. issue 1. 1993-06-09. PMID:7683543. |
in this study, we have demonstrated that regulation of hs578t igfbp-3 by igf peptides is specific, non-receptor mediated, and post-translational by showing: 1) dose-dependent increase of igfbp-3 in conditioned media(cm) following addition of igf-i and igf-ii (maximum 8-13-fold increase at 100 ng/ml concentration), but not by insulin up to 1 microgram/ml; 2) confirmation of igf-induced increases in cm concentrations of igfbp-3 by means of western ligand blot, affinity cross-linking, and igfbp-3-specific radioimmunoassay; 3) increase of igfbp-3 in cm by addition of igf analogs which retain full affinity for igfbps ([leu27]igf-ii and [tyr55,gln56]igf-i), but not by igf analogs which have significantly decreased affinity for igfbps ([gln3,ala4,tyr15,leu16]igf-i, [gln6,ala7,tyr18,leu19,leu27]igf-ii and igf-i/insulin hybrid); 4) no change in igfbp-3 mrna following addition of igfs; 5) existence of metal ion-dependent igfbp-3 specific protease in cm and protection of igfbp-3 from protease by formation of [igf:igfbp-3] complexes; and 6) release of cell surface-associated igfbp-3 into cm by addition of igf peptides. |
1993-06-09 |
2023-08-12 |
human |
| J A Flores, J C Garmey, J E Nestler, J D Veldhui. Sites of inhibition of steroidogenesis by activation of protein kinase-C in swine ovarian (granulosa) cells. Endocrinology. vol 132. issue 5. 1993-05-25. PMID:8477649. |
cellular concentrations of p450scc mrna, as measured by northern blot hybridization with a 32p-labeled 1-kilobase porcine cdna clone, were significantly increased by insulin. |
1993-05-25 |
2023-08-12 |
Not clear |
| S U Devaskar, H F Sadiq, L Holtzclaw, M Georg. The developmental pattern of rabbit brain insulin and insulin-like growth factor receptor expression. Brain research. vol 605. issue 1. 1993-05-10. PMID:8467380. |
to examine the effect of development on rabbit brain insulin and insulin-like growth factor (igf) receptor expression, we characterized and quantitated receptor mrnas by northern blot analysis and affinity-labeled ligand bound receptors by sds-page and autoradiography. |
1993-05-10 |
2023-08-12 |
rabbit |
| Y Fujii, K Hasumi, H Nakamura, Y Takahama, H Shimizu, I Sugawara, M Eriguchi, M Sekiguch. Characterization of a murine monoclonal antibody, 5D-4, reacting with pancreatic cancers and islets of Langerhans. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 46. issue 9. 1993-04-07. PMID:1292652. |
the 5d-4 mab reacted not only with ca 50 kda and 30 kda solubilized protein from aspc-1 cells by western blot analysis but also with human insulin in a competition ria. |
1993-04-07 |
2023-08-11 |
mouse |
| M C Hammond-Kosack, M W Kilpatrick, K Dochert. Analysis of DNA structure in the human insulin gene-linked polymorphic region in vivo. Journal of molecular endocrinology. vol 9. issue 3. 1993-02-05. PMID:1476609. |
following digestion with taqi and subsequent digestion with s1-nuclease to cleave at the bromoacetaldehyde-reactive sites, the dna was subjected to agarose gel electrophoresis, and insulin gene fragments were detected by southern blot analysis. |
1993-02-05 |
2023-08-11 |
human |
| N Sun, Z Su. [Effects of insulin on expression of oncogenes in CWE mouse brain]. Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao. vol 23. issue 2. 1993-01-07. PMID:1452149. |
by rna dot blot hybridization, we observed the expression of c-myc, c-fos, ha-ras and erbb genes in normal brain of cwe mouse after the injection of insulin. |
1993-01-07 |
2023-08-11 |
mouse |
| J Fowlkes, M Freemar. Evidence for a novel insulin-like growth factor (IGF)-dependent protease regulating IGF-binding protein-4 in dermal fibroblasts. Endocrinology. vol 131. issue 5. 1992-12-01. PMID:1385096. |
to determine whether the effects of igfs on igfbp-4 might be exerted through alterations in igfbp-4 degradation, we incubated cell-free, fibroblast-conditioned media from either sheep or human dermal fibroblasts with or without igf-i, igf-ii (each 1 microgram/ml), or insulin (10 micrograms/ml) for 72 h at 37 c. samples were then analyzed by western ligand blot using radiolabeled igfs and by immunoblotting using a polyclonal antisera to human igfbp-4. |
1992-12-01 |
2023-08-11 |
human |
| A Kanatsuka, H Makino, T Yamaguchi, H Ohsawa, Y Tokuyama, T Saitoh, K Yamamura, J Miyazaki, S Yoshid. Islet amyloid polypeptide/amylin in pancreatic beta-cell line derived from transgenic mouse insulinoma. Diabetes. vol 41. issue 11. 1992-11-19. PMID:1383068. |
rna blot analysis revealed an abundance of iapp and insulin ii mrna in the cells, findings comparable with those in the pancreas of a normal mouse. |
1992-11-19 |
2023-08-11 |
mouse |
| Y J Lin, S Harada, E G Loten, R M Smith, L Jaret. Direct stimulation of immediate-early genes by intranuclear insulin in trypsin-treated H35 hepatoma cells. Proceedings of the National Academy of Sciences of the United States of America. vol 89. issue 20. 1992-11-17. PMID:1409684. |
northern blot analysis revealed insulin increased g33 and c-fos mrna concentrations identically in control and trypsin-treated cells but had no effect on beta 2-microglobulin mrna. |
1992-11-17 |
2023-08-11 |
Not clear |
| R Yuasa, M C Eggo, J Meinkoth, W H Dillmann, G N Burro. Iodide induces transforming growth factor beta 1 (TGF-beta 1) mRNA in sheep thyroid cells. Thyroid : official journal of the American Thyroid Association. vol 2. issue 2. 1992-10-16. PMID:1525582. |
thyroid cells were incubated with tsh and five additives (insulin, somatostatin, growth hormone, transferrin, and glycyl-l-histidyl-l-lysin) for 2-3 weeks and then were exposed to sodium iodide (nai) or 1-methylimidazole-2-thiol (methimazole, mmi), or both for 72 h. iodide at 10(-6) m and 10(-4) m significantly increased the amount of tgf-beta mrna as determined by northern blot analysis with a rat tgf-beta 1 cdna probe. |
1992-10-16 |
2023-08-11 |
rat |
| M A Leal, C Cabañas, C Rius, P Aller, C Call. Modulation by dexamethasone of insulin binding and insulin receptor mRNA levels in U-937 human promonocytic cells. Biochimie. vol 74. issue 6. 1992-10-14. PMID:1520733. |
northern blot assays revealed two insulin receptor-related mrnas of approximately 11 and 7 kb in size. |
1992-10-14 |
2023-08-11 |
human |
| G W Gould, A M Brant, B B Kahn, P R Shepherd, S C McCoid, E M Gibb. Expression of the brain-type glucose transporter is restricted to brain and neuronal cells in mice. Diabetologia. vol 35. issue 4. 1992-10-06. PMID:1516757. |
northern blot analysis of human tissues has demonstrated the expression of the brain-type glucose transporter isoform (glut 3) in liver, muscle and fat, raising the possibility that this transporter isoform may play a role in the regulation of glucose disposal in these tissues in response to insulin. |
1992-10-06 |
2023-08-11 |
mouse |
| R Schechter, J Whitmire, L Holtzclaw, M George, R Harlow, S U Devaska. Developmental regulation of insulin in the mammalian central nervous system. Brain research. vol 582. issue 1. 1992-09-14. PMID:1482442. |
employing porcine/bovine or rabbit insulin as standards, we observed that brain insulin mimicked authentic insulin in its migration on sds-polyacrylamide and native gel electrophoresis, immunogenicity on western blot analysis, and its elution profile on immunoaffinity column chromatographic, and high performance liquid chromatographic separation. |
1992-09-14 |
2023-08-11 |
rabbit |
| R Schechter, J Whitmire, L Holtzclaw, M George, R Harlow, S U Devaska. Developmental regulation of insulin in the mammalian central nervous system. Brain research. vol 582. issue 1. 1992-09-14. PMID:1482442. |
a search for insulin mrna by northern blot analysis yielded minute amounts of atypical large sized transcripts. |
1992-09-14 |
2023-08-11 |
rabbit |
| S A Dekowski, J M Snyde. Insulin regulation of messenger ribonucleic acid for the surfactant-associated proteins in human fetal lung in vitro. Endocrinology. vol 131. issue 2. 1992-09-02. PMID:1639013. |
in the present study, we used northern blot analysis to evaluate the effects of insulin on the content of sp-a messenger rna (mrna) as well as on the content of mrna for the hydrophobic surfactant-associated proteins sp-b and sp-c in human fetal lung explants maintained in vitro. |
1992-09-02 |
2023-08-11 |
human |
| B Thorens, Y J Wu, J L Leahy, G C Wei. The loss of GLUT2 expression by glucose-unresponsive beta cells of db/db mice is reversible and is induced by the diabetic environment. The Journal of clinical investigation. vol 90. issue 1. 1992-08-24. PMID:1634622. |
glucose-induced insulin secretion by beta cells of diabetic db/db mice was studied by a pancreas perfusion technique, and the levels of glut2 protein in pancreatic islets were assessed by immunofluorescence microscopy and protein blot analysis. |
1992-08-24 |
2023-08-11 |
mouse |
| B Bhandari, K S Saini, R E Mille. Glycerol 3-phosphate dehydrogenase gene expression in cultured 3T3-L1 adipocytes: regulation by insulin, dexamethasone and dibutyryl cAMP at the level of mRNA abundance, transcription and mRNA stability. Molecular and cellular endocrinology. vol 76. issue 1-3. 1992-08-11. PMID:1668204. |
an increase by insulin (4-fold) and decrease by dexamethasone (by 50%) and dibutyryl camp (by 70%) was observed for g3pdh mrna abundance as analyzed by northern blot hybridization. |
1992-08-11 |
2023-08-11 |
human |