| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| K Hawrami, R Mohan, V Mohan, G A Hitma. A genetic study of retinopathy in south Indian type 2 (non-insulin-dependent) diabetic patients. Diabetologia. vol 34. issue 6. 1991-10-08. PMID:1679401. |
dna was extracted from blood samples and studied by southern blot hybridisation techniques and the following probe enzyme combinations: hla-dqb1; taq 1, hla-dqa1; taq 1, hla-dra; bgl ii, insulin gene hypervariable region; pvu ii and the switch region of the immunoglobulin igm heavy chain gene (s mu); sac i. |
1991-10-08 |
2023-08-11 |
Not clear |
| J F Wang, G P Becks, E Hanada, K D Buckingham, I D Phillips, D J Hil. Hormonal regulation of insulin-like growth factor (IGF)-binding proteins secreted by isolated sheep thyroid epithelial cells: relationship with iodine organification. The Journal of endocrinology. vol 130. issue 1. 1991-10-03. PMID:1715378. |
sheep thyroid follicles were isolated by incubation with collagenase and differential centrifugation, grown in coon's modified ham's f12m medium with the addition of transferrin, glycylhistidyl-lysine, somatostatin (3h), tsh, cortisol and insulin (6h), and maintained in oh (hormone-free) or 3h medium with or without further supplements for 48 h. conditioned culture medium was separated by 8% sodium dodecyl sulphate (sds)-polyacrylamide gel electrophoresis, transferred to nitrocellulose and incubated with 125i-labelled igf-ii followed by autoradiography (ligand blot). |
1991-10-03 |
2023-08-11 |
Not clear |
| I Hatayama, Y Yamada, K Tanaka, A Ichihara, K Sat. Induction of glutathione S-transferase P-form in primary cultured rat hepatocytes by epidermal growth factor and insulin. Japanese journal of cancer research : Gann. vol 82. issue 7. 1991-10-01. PMID:1908848. |
western blot analysis of gsts revealed that gst-p, which is not present in freshly isolated hepatocytes, was markedly induced and that gst subunits 3 and 4 of the mu class also increased after addition of egf and/or insulin, while the subunits 1 and 2 of the alpha class disappeared. |
1991-10-01 |
2023-08-11 |
rat |
| I Hatayama, Y Yamada, K Tanaka, A Ichihara, K Sat. Induction of glutathione S-transferase P-form in primary cultured rat hepatocytes by epidermal growth factor and insulin. Japanese journal of cancer research : Gann. vol 82. issue 7. 1991-10-01. PMID:1908848. |
northern blot analysis showed that on addition of egf and insulin the level of gst-p mrna was also elevated and expressions of the nuclear oncogenes c-jun and c-fos were enhanced. |
1991-10-01 |
2023-08-11 |
rat |
| S Nagamatsu, M Nishi, D F Steine. Biosynthesis of islet amyloid polypeptide. Elevated expression in mouse beta TC3 cells. The Journal of biological chemistry. vol 266. issue 21. 1991-08-23. PMID:1856207. |
northern blot analysis revealed that the size of iapp mrna (0.9 kb) in beta tc3 cells was the same as that in normal mouse islets; iapp mrna was approximately 60% of the level of insulin mrna in beta tc3 cells. |
1991-08-23 |
2023-08-11 |
mouse |
| T G Unterman, D T Oehler, L J Murphy, R G Lacso. Multihormonal regulation of insulin-like growth factor-binding protein-1 in rat H4IIE hepatoma cells: the dominant role of insulin. Endocrinology. vol 128. issue 6. 1991-07-02. PMID:1709855. |
northern and slot blot analyses revealed that 100 nm insulin profoundly lowered the abundance of igfbp-1 mrna in h4iie cells (4 +/- 0.6% of control at 4 h; p less than 0.001), while igfbp-1 mrna was increased 2-fold during incubation with 75 microm 8-cpt-camp (p less than 0.001) and 9-fold with 1 microm dexamethasone (p less than 0.001). |
1991-07-02 |
2023-08-11 |
rat |
| T G Unterman, D T Oehler, L J Murphy, R G Lacso. Multihormonal regulation of insulin-like growth factor-binding protein-1 in rat H4IIE hepatoma cells: the dominant role of insulin. Endocrinology. vol 128. issue 6. 1991-07-02. PMID:1709855. |
to determine whether this effect of insulin reflected altered generation or stability of igfbp-1 mrna, h4iie cells were incubated with 2.5 micrograms/ml actinomycin-d with or without insulin, and mrna was quantitated by northern blot. |
1991-07-02 |
2023-08-11 |
rat |
| N Feuerstein, P A Randazz. In vivo and in vitro phosphorylation studies of numatrin, a cell cycle regulated nuclear protein, in insulin-stimulated NIH 3T3 HIR cells. Experimental cell research. vol 194. issue 2. 1991-06-07. PMID:2026181. |
we found that an increase in phosphorylation of numatrin was associated with stimulation of the cells with insulin for 4 h and that the level of phosphorylation remained elevated after 8 h. by this time there was no increase in numatrin abundance as shown by coom-massie blue stain and western blot analysis. |
1991-06-07 |
2023-08-11 |
Not clear |
| A M Rohilla, C Anderson, W M Wood, P Berhan. Insulin downregulates the steady-state level of its receptor's messenger ribonucleic acid. Biochemical and biophysical research communications. vol 175. issue 2. 1991-05-23. PMID:2018500. |
the effects of insulin on the steady-state level of human insulin receptor (hir) mrna were examined in the hepg2 human liver cell line using northern blot analysis of either total cellular or poly(a)+ rna. |
1991-05-23 |
2023-08-11 |
human |
| L K Bachrach, M C Eggo, G N Burrow, F Liu, T Tram, D R Powel. Regulation of insulin-like growth factor-binding protein messenger ribonucleic acid levels in sheep thyroid cells. Endocrinology. vol 128. issue 4. 1991-04-23. PMID:1706262. |
cells grown in basal medium or with six-hormone (6h) medium supplements (transferrin, glycyl-histidyl-lysine, hydrocortisone, somatostatin, insulin, and tsh) release nonglycosylated bps that migrate at 24, 27, 29, and 32 kda on western ligand blot. |
1991-04-23 |
2023-08-11 |
rat |
| P Shier, H F Willard, V M Wat. Localization of the insulin receptor-related receptor gene to human chromosome 1. Cytogenetics and cell genetics. vol 54. issue 1-2. 1991-01-16. PMID:2249481. |
dna encoding the insulin receptor-related receptor (irr), a novel receptor whose predicted primary structure is similar to those of the insulin and insulin-like growth factor i receptors, has been used in southern blot analysis of dna from human x mouse somatic cell hybrids to assign the irr gene (insrr) to human chromosome 1. |
1991-01-16 |
2023-08-11 |
mouse |
| K M Thraikill, D R Clemmons, W H Busby, S Handwerge. Differential regulation of insulin-like growth factor binding protein secretion from human decidual cells by IGF-I, insulin, and relaxin. The Journal of clinical investigation. vol 86. issue 3. 1990-10-11. PMID:1697605. |
using ligand blot analysis and an ria for the 30,000-mr form (igf-bp-1), we examined the effects of igf-i (10-1,000 ng/ml), insulin (10-10,000 ng/ml), and relaxin (10-250 ng/ml) on decidual cell igf-bp release after 120 h of hormone exposure. |
1990-10-11 |
2023-08-11 |
human |
| D Thiebaud, K W Ng, D M Findlay, M Harker, T J Marti. Insulinlike growth factor 1 regulates mRNA levels of osteonectin and pro-alpha 1(I)-collagen in clonal preosteoblastic calvarial cells. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. vol 5. issue 7. 1990-10-10. PMID:2204253. |
cells were treated for 24 h with insulin, growth hormone, or igf-1 to study the regulation of messenger rna for osteonectin and pro-alpha 1(i)-collagen using northern blot hybridization. |
1990-10-10 |
2023-08-11 |
rat |
| S P LeDoux, N J Patton, J W Nelson, W A Bohr, G L Wilso. Preferential DNA repair of alkali-labile sites within the active insulin gene. The Journal of biological chemistry. vol 265. issue 25. 1990-10-09. PMID:2203764. |
quantitative dna blot analysis was utilized to assess the formation and repair of alkali-labile sites within the restriction fragment containing the insulin gene. |
1990-10-09 |
2023-08-11 |
rat |
| C J Williams, D H Wreschner, A Tanaka, I Tsarfaty, I Keydar, A S Dio. Multiple protein forms of the human breast tumor-associated epithelial membrane antigen (EMA) are generated by alternative splicing and induced by hormonal stimulation. Biochemical and biophysical research communications. vol 170. issue 3. 1990-09-27. PMID:2202302. |
the bt549 line has been shown to exhibit enhanced expression and secretion of ema when the cells are cultivated in a medium supplemented with hydrocortisone and insulin, and northern blot analysis demonstrated that ema-related rna transcripts are commensurately enhanced. |
1990-09-27 |
2023-08-11 |
human |
| P R Johnson, B Spiegelman, B Rosen, I Turkenkopf, H Ree, M R Greenwoo. Reduced adipsin mRNA and circulating adipsin protein are modulated by adrenal steroids in obese Zucker rats. The American journal of physiology. vol 259. issue 1 Pt 2. 1990-08-30. PMID:2197880. |
in both experiments, serum adipsin protein was determined by western blot analysis and plasma insulin by radioimmunoassay. |
1990-08-30 |
2023-08-11 |
rat |
| M K Sinha, C Buchanan, C Raineri-Maldonado, P Khazanie, S Atkinson, R DiMarchi, J F Car. IGF-II receptors and IGF-II-stimulated glucose transport in human fat cells. The American journal of physiology. vol 258. issue 3 Pt 1. 1990-04-26. PMID:2156441. |
through the use of 0.5 mm disuccinimidyl suberate, 125i-igf-ii is cross-linked to a 260-kda protein that is identified as the igf-ii receptor by displacement experiments with unlabeled igf-ii, igf-i, and insulin and either by immunoprecipitation or by western blot analysis with mannose 6-phosphate receptor antibodies. |
1990-04-26 |
2023-08-11 |
human |
| G D Chazenbalk, H L Wadsworth, B Rapopor. Transcriptional regulation of ferritin H messenger RNA levels in FRTL5 rat thyroid cells by thyrotropin. The Journal of biological chemistry. vol 265. issue 2. 1990-02-21. PMID:2153109. |
on northern blot analysis, tsh (in the presence of serum and insulin) increased ferritin h mrna levels, with an initial response evident after 1 h of stimulation. |
1990-02-21 |
2023-08-11 |
rat |
| L K Bachrach, F R Liu, G N Burrow, M C Egg. Characterization of insulin-like growth factor-binding proteins from sheep thyroid cells. Endocrinology. vol 125. issue 6. 1990-01-09. PMID:2479527. |
cells grown without or with standard medium supplements (transferrin, glycyl-histidyl-lysine, hydrocortisone, somatostatin, insulin, and tsh) released binding proteins with apparent mol wt of 23, 29, and 32 kda on western ligand blot (nonreduced). |
1990-01-09 |
2023-08-11 |
human |
| L C Andersen, B Michelsen, O D Madse. Molecular characterization of size and copy number of the human insulin gene in transfected rat islet tumor cell clones. Experimental and clinical endocrinology. vol 93. issue 2-3. 1989-10-20. PMID:2570709. |
two stable transfectants, nhi-6f and nhi-5b, obtained by cotransfection of the human insulin gene (a 15 kb ecori genomic fragment) with psv2-neo into msl-cells (pluripotent rat islet tumor cells), were characterized with regard to copy number and size of the inserted human sequences by southern blot analyses using appropriate restriction enzymes. |
1989-10-20 |
2023-08-11 |
human |