| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| P J Miettinen, K Heikinheim. Transforming growth factor-alpha (TGF-alpha) and insulin gene expression in human fetal pancreas. Development (Cambridge, England). vol 114. issue 4. 1992-07-31. PMID:1618147. |
in northern blot analysis a single band of 4.8 kilobases (kb) corresponding to the tgf-alpha transcript and a 0.6 kb for the insulin mrna were detected in the pancreas. |
1992-07-31 |
2023-08-11 |
human |
| M E Thompson, W E Zimmer, A L Haynes, D L Valentine, S Forss-Petter, J G Scammel. Prolactin granulogenesis is associated with increased secretogranin expression and aggregation in the Golgi apparatus of GH4C1 cells. Endocrinology. vol 131. issue 1. 1992-07-27. PMID:1612012. |
gh cells were treated for up to 3 days with 17 beta-estradiol (1 nm), insulin (300 nm), and epidermal growth factor (10 nm) and were fixed in 4% paraformaldehyde for immunocytochemistry or harvested for rna isolation and northern blot analysis. |
1992-07-27 |
2023-08-11 |
Not clear |
| P A Farrell, A L Caston, D Rodd, J Engdah. Effect of training on insulin secretion from single pancreatic beta cells. Medicine and science in sports and exercise. vol 24. issue 4. 1992-05-14. PMID:1560738. |
in vitro insulin secretion from single beta cells was measured using the cell blot assay (kendall and hymer, endocrinology, 121:2260-2262, 1987) and t, had lower secretion 2.6 +/- 1.4 pg.cell-1 when compared (p less than 0.05) with s, 6.7 +/- 0.6, but not lower than pf, 4.1 +/- 1.7 pg.cell-1. |
1992-05-14 |
2023-08-11 |
rat |
| S P Campos, H Bauman. Insulin is a prominent modulator of the cytokine-stimulated expression of acute-phase plasma protein genes. Molecular and cellular biology. vol 12. issue 4. 1992-04-22. PMID:1372389. |
quantitation of c/ebp beta mrna by northern (rna) blot analysis and of c/ebp beta dna binding activity by southwestern (dna-protein) blot analysis showed that insulin, when combined with cytokines and dexamethasone, stimulated both the mrna and dna binding activity by a factor of 1.6 compared with that of cells treated with cytokines and dexamethasone alone. |
1992-04-22 |
2023-08-11 |
human |
| S Nagata, K Inoue, T Sensh. Estrogen-insulin synergism in induction of prolactin in growth hormone-producing cells. Molecular and cellular endocrinology. vol 82. issue 2-3. 1992-04-07. PMID:1794611. |
incorporation of [35s]amino acids into immunoprecipitable prl became detectable at 15 h of culture with insulin (500 ng/ml) and increased up to 48 h. northern blot hybridization demonstrated a concurrent induction of prl mrna. |
1992-04-07 |
2023-08-11 |
Not clear |
| F J Oliver, G de la Rubia, E P Feener, M E Lee, M R Loeken, T Shiba, T Quertermous, G L Kin. Stimulation of endothelin-1 gene expression by insulin in endothelial cells. The Journal of biological chemistry. vol 266. issue 34. 1992-01-13. PMID:1744120. |
by rna blot analysis, insulin (1.67 x 10(-8) m) increased et-1 mrna levels by 2.3-fold over the basal within 10 min and attained a maximum (5.3-fold increase) in 2 h. dose-response studies showed that a maximum effect of insulin was reached at 1.67 x 10(-8) m although a significant increase can be observed at 1.66 x 10(-9) m. radioligand receptor studies indicated that the affinity constant for insulin receptors on endothelial cells correlated closely with the dose response observed for et-1 mrna. |
1992-01-13 |
2023-08-11 |
cat |
| K Hawrami, R Mohan, V Mohan, G A Hitma. A genetic study of retinopathy in south Indian type 2 (non-insulin-dependent) diabetic patients. Diabetologia. vol 34. issue 6. 1991-10-08. PMID:1679401. |
dna was extracted from blood samples and studied by southern blot hybridisation techniques and the following probe enzyme combinations: hla-dqb1; taq 1, hla-dqa1; taq 1, hla-dra; bgl ii, insulin gene hypervariable region; pvu ii and the switch region of the immunoglobulin igm heavy chain gene (s mu); sac i. |
1991-10-08 |
2023-08-11 |
Not clear |
| J F Wang, G P Becks, E Hanada, K D Buckingham, I D Phillips, D J Hil. Hormonal regulation of insulin-like growth factor (IGF)-binding proteins secreted by isolated sheep thyroid epithelial cells: relationship with iodine organification. The Journal of endocrinology. vol 130. issue 1. 1991-10-03. PMID:1715378. |
sheep thyroid follicles were isolated by incubation with collagenase and differential centrifugation, grown in coon's modified ham's f12m medium with the addition of transferrin, glycylhistidyl-lysine, somatostatin (3h), tsh, cortisol and insulin (6h), and maintained in oh (hormone-free) or 3h medium with or without further supplements for 48 h. conditioned culture medium was separated by 8% sodium dodecyl sulphate (sds)-polyacrylamide gel electrophoresis, transferred to nitrocellulose and incubated with 125i-labelled igf-ii followed by autoradiography (ligand blot). |
1991-10-03 |
2023-08-11 |
Not clear |
| I Hatayama, Y Yamada, K Tanaka, A Ichihara, K Sat. Induction of glutathione S-transferase P-form in primary cultured rat hepatocytes by epidermal growth factor and insulin. Japanese journal of cancer research : Gann. vol 82. issue 7. 1991-10-01. PMID:1908848. |
western blot analysis of gsts revealed that gst-p, which is not present in freshly isolated hepatocytes, was markedly induced and that gst subunits 3 and 4 of the mu class also increased after addition of egf and/or insulin, while the subunits 1 and 2 of the alpha class disappeared. |
1991-10-01 |
2023-08-11 |
rat |
| I Hatayama, Y Yamada, K Tanaka, A Ichihara, K Sat. Induction of glutathione S-transferase P-form in primary cultured rat hepatocytes by epidermal growth factor and insulin. Japanese journal of cancer research : Gann. vol 82. issue 7. 1991-10-01. PMID:1908848. |
northern blot analysis showed that on addition of egf and insulin the level of gst-p mrna was also elevated and expressions of the nuclear oncogenes c-jun and c-fos were enhanced. |
1991-10-01 |
2023-08-11 |
rat |
| S Nagamatsu, M Nishi, D F Steine. Biosynthesis of islet amyloid polypeptide. Elevated expression in mouse beta TC3 cells. The Journal of biological chemistry. vol 266. issue 21. 1991-08-23. PMID:1856207. |
northern blot analysis revealed that the size of iapp mrna (0.9 kb) in beta tc3 cells was the same as that in normal mouse islets; iapp mrna was approximately 60% of the level of insulin mrna in beta tc3 cells. |
1991-08-23 |
2023-08-11 |
mouse |
| T G Unterman, D T Oehler, L J Murphy, R G Lacso. Multihormonal regulation of insulin-like growth factor-binding protein-1 in rat H4IIE hepatoma cells: the dominant role of insulin. Endocrinology. vol 128. issue 6. 1991-07-02. PMID:1709855. |
northern and slot blot analyses revealed that 100 nm insulin profoundly lowered the abundance of igfbp-1 mrna in h4iie cells (4 +/- 0.6% of control at 4 h; p less than 0.001), while igfbp-1 mrna was increased 2-fold during incubation with 75 microm 8-cpt-camp (p less than 0.001) and 9-fold with 1 microm dexamethasone (p less than 0.001). |
1991-07-02 |
2023-08-11 |
rat |
| T G Unterman, D T Oehler, L J Murphy, R G Lacso. Multihormonal regulation of insulin-like growth factor-binding protein-1 in rat H4IIE hepatoma cells: the dominant role of insulin. Endocrinology. vol 128. issue 6. 1991-07-02. PMID:1709855. |
to determine whether this effect of insulin reflected altered generation or stability of igfbp-1 mrna, h4iie cells were incubated with 2.5 micrograms/ml actinomycin-d with or without insulin, and mrna was quantitated by northern blot. |
1991-07-02 |
2023-08-11 |
rat |
| N Feuerstein, P A Randazz. In vivo and in vitro phosphorylation studies of numatrin, a cell cycle regulated nuclear protein, in insulin-stimulated NIH 3T3 HIR cells. Experimental cell research. vol 194. issue 2. 1991-06-07. PMID:2026181. |
we found that an increase in phosphorylation of numatrin was associated with stimulation of the cells with insulin for 4 h and that the level of phosphorylation remained elevated after 8 h. by this time there was no increase in numatrin abundance as shown by coom-massie blue stain and western blot analysis. |
1991-06-07 |
2023-08-11 |
Not clear |
| A M Rohilla, C Anderson, W M Wood, P Berhan. Insulin downregulates the steady-state level of its receptor's messenger ribonucleic acid. Biochemical and biophysical research communications. vol 175. issue 2. 1991-05-23. PMID:2018500. |
the effects of insulin on the steady-state level of human insulin receptor (hir) mrna were examined in the hepg2 human liver cell line using northern blot analysis of either total cellular or poly(a)+ rna. |
1991-05-23 |
2023-08-11 |
human |
| L K Bachrach, M C Eggo, G N Burrow, F Liu, T Tram, D R Powel. Regulation of insulin-like growth factor-binding protein messenger ribonucleic acid levels in sheep thyroid cells. Endocrinology. vol 128. issue 4. 1991-04-23. PMID:1706262. |
cells grown in basal medium or with six-hormone (6h) medium supplements (transferrin, glycyl-histidyl-lysine, hydrocortisone, somatostatin, insulin, and tsh) release nonglycosylated bps that migrate at 24, 27, 29, and 32 kda on western ligand blot. |
1991-04-23 |
2023-08-11 |
rat |
| P Shier, H F Willard, V M Wat. Localization of the insulin receptor-related receptor gene to human chromosome 1. Cytogenetics and cell genetics. vol 54. issue 1-2. 1991-01-16. PMID:2249481. |
dna encoding the insulin receptor-related receptor (irr), a novel receptor whose predicted primary structure is similar to those of the insulin and insulin-like growth factor i receptors, has been used in southern blot analysis of dna from human x mouse somatic cell hybrids to assign the irr gene (insrr) to human chromosome 1. |
1991-01-16 |
2023-08-11 |
mouse |
| K M Thraikill, D R Clemmons, W H Busby, S Handwerge. Differential regulation of insulin-like growth factor binding protein secretion from human decidual cells by IGF-I, insulin, and relaxin. The Journal of clinical investigation. vol 86. issue 3. 1990-10-11. PMID:1697605. |
using ligand blot analysis and an ria for the 30,000-mr form (igf-bp-1), we examined the effects of igf-i (10-1,000 ng/ml), insulin (10-10,000 ng/ml), and relaxin (10-250 ng/ml) on decidual cell igf-bp release after 120 h of hormone exposure. |
1990-10-11 |
2023-08-11 |
human |
| D Thiebaud, K W Ng, D M Findlay, M Harker, T J Marti. Insulinlike growth factor 1 regulates mRNA levels of osteonectin and pro-alpha 1(I)-collagen in clonal preosteoblastic calvarial cells. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. vol 5. issue 7. 1990-10-10. PMID:2204253. |
cells were treated for 24 h with insulin, growth hormone, or igf-1 to study the regulation of messenger rna for osteonectin and pro-alpha 1(i)-collagen using northern blot hybridization. |
1990-10-10 |
2023-08-11 |
rat |
| S P LeDoux, N J Patton, J W Nelson, W A Bohr, G L Wilso. Preferential DNA repair of alkali-labile sites within the active insulin gene. The Journal of biological chemistry. vol 265. issue 25. 1990-10-09. PMID:2203764. |
quantitative dna blot analysis was utilized to assess the formation and repair of alkali-labile sites within the restriction fragment containing the insulin gene. |
1990-10-09 |
2023-08-11 |
rat |