| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| B F Lindgren, I Odar-Cederlöf, F Ericsson, K Brisma. Decreased bioavailability of insulin-like growth factor-I, a cause of catabolism in hemodialysis patients? Growth regulation. vol 6. issue 3. 1997-05-16. PMID:8894646. |
in esrd patients predialysis mean values of insulin, igf-i sd-score, igfbp-1 and igfbp-2 were increased, while the mean densitrometric values of the igfbp-3 bands on western ligand blot were either normal or reduced. |
1997-05-16 |
2023-08-12 |
Not clear |
| J F Wang, G P Becks, D J Hil. Degradation of IGF-binding protein-3 by proteases in cultured FRTL-5 rat thyroid cells. The Journal of endocrinology. vol 152. issue 2. 1997-04-02. PMID:9071984. |
when frtl-5 rat thyroid cells were cultured in 6h medium (modified f-12m medium containing tsh, insulin, hydrocortisone, somatostatin, transferrin, and glycyl-histidyl-lysine) containing 5% calf serum, both 44-46 and 31 kda igfbps were found in conditioned medium by ligand blot analysis using 125i-labelled igf-ii. |
1997-04-02 |
2023-08-12 |
human |
| I Vietor, M Rusnak, E Viskupic, P Blazicek, E L Sabban, R Kvetnansk. Glucoprivation by insulin leads to trans-synaptic increase in rat adrenal tyrosine hydroxylase mRNA levels. European journal of pharmacology. vol 313. issue 1-2. 1997-02-27. PMID:8905338. |
western blot analysis showed that although a single insulin treatment did not affect tyrosine hydroxylase protein levels, a significant increase was observed following the seventh administration. |
1997-02-27 |
2023-08-12 |
rat |
| D Lu, H Hoshino, T Takeuch. Regulatable production of mature insulin from a hepatocyte cell line: insulin production is up-regulated by cAMP and glucocorticoids, and down-regulated by insulin. FEBS letters. vol 399. issue 1-2. 1997-01-31. PMID:8980115. |
addition of exogenous insulin to the culture medium significantly decreased insulin mrna expression on northern blot. |
1997-01-31 |
2023-08-12 |
rat |
| S Harada, R M Smith, J A Smith, M F White, L Jaret. Insulin-induced egr-1 and c-fos expression in 32D cells requires insulin receptor, Shc, and mitogen-activated protein kinase, but not insulin receptor substrate-1 and phosphatidylinositol 3-kinase activation. The Journal of biological chemistry. vol 271. issue 47. 1997-01-13. PMID:8939974. |
insulin (17 nm) increased egr-1 and c-fos expression in 32d/ir and 32d/ir+irs cells, but not in parental cells or 32d/irs cells, as determined by northern blot analysis. |
1997-01-13 |
2023-08-12 |
Not clear |
| S Harada, R M Smith, J A Smith, M F White, L Jaret. Insulin-induced egr-1 and c-fos expression in 32D cells requires insulin receptor, Shc, and mitogen-activated protein kinase, but not insulin receptor substrate-1 and phosphatidylinositol 3-kinase activation. The Journal of biological chemistry. vol 271. issue 47. 1997-01-13. PMID:8939974. |
insulin treatment (5 min at 37 degrees c) markedly increased tyrosine phosphorylation of several proteins, including the insulin receptor, irs-1, and shc, in 32d/ir+irs cells as determined by immunoprecipitation and western blot analysis with anti-phosphotyrosine antibody. |
1997-01-13 |
2023-08-12 |
Not clear |
| D B Schranz, A M Rohilla, C Anderson, W M Wood, P Berhan. Insulin internalization in the absence of the insulin receptor tyrosine kinase domain is insufficient for mediating intracellular biological effects. Biochemical and biophysical research communications. vol 227. issue 2. 1996-12-03. PMID:8878559. |
the hir delta 343 receptors retained the ability to internalize a significant amount of surface-bound insulin at 37 degrees c. however, they were unable to mediate either the short or long-term biological effects of insulin as determined by assaying insulin-stimulated glucose uptake (assessed by 2-deoxyglucose uptake), protooncogene expression (measured by northern blot analysis of c-fos mrna) and dna synthesis (measured by 3h-thymidine incorporation). |
1996-12-03 |
2023-08-12 |
human |
| U Myrsén, B Ahrén, F Sundle. Dexamethasone-induced neuropeptide Y expression in rat islet endocrine cells. Rapid reversibility and partial prevention by insulin. Diabetes. vol 45. issue 10. 1996-11-04. PMID:8826964. |
the present study, using icc, ish, and northern blot, addressed the question whether the islet cell expression of npy induced by dex is affected by concomitant insulin (60 u/kg body wt daily for 12 days) treatment. |
1996-11-04 |
2023-08-12 |
rat |
| Y Anouar, L E Eide. Rapid and long-lasting increase in galanin mRNA levels in rat adrenal medulla following insulin-induced reflex splanchnic nerve stimulation. Neuroendocrinology. vol 62. issue 6. 1996-10-24. PMID:8751287. |
here, we examine the cellular localization and mechanism of galanin mrna upregulation in the adrenal gland after insulin administration, by northern blot and in situ histochemical hybridization analysis. |
1996-10-24 |
2023-08-12 |
rat |
| Y Shimizu, D Kielar, Y Minokoshi, T Shimaz. Noradrenaline increases glucose transport into brown adipocytes in culture by a mechanism different from that of insulin. The Biochemical journal. vol 314 ( Pt 2). 1996-08-05. PMID:8670061. |
furthermore western blot analysis with an anti-phosphotyrosine antibody revealed that, in contrast with insulin, noradrenaline apparently does not stimulate intracellular phosphorylation of tyrosine, suggesting that the noradrenaline-induced increase in dglc uptake depends on elevation of the intracellular cyclic amp level and not on the signal chain common to insulin. |
1996-08-05 |
2023-08-12 |
Not clear |
| C Reynet, C R Kahn, M R Loeke. Expression of the gene encoding glycogen phosphorylase is elevated in diabetic rat skeletal muscle and is regulated by insulin and cyclic AMP. Diabetologia. vol 39. issue 2. 1996-07-10. PMID:8635670. |
insulin stimulated the accumulation of glycogen phosphorylase mrna as determined by northern blot analysis. |
1996-07-10 |
2023-08-12 |
rat |
| T Ohno, C Ishii, N Kato, Y Ito, M Shimizu, S Tomono, K Murata, S Kawaz. Increased expression of a regenerating (reg) gene protein in neonatal rat pancreas treated with streptozotocin. Endocrine journal. vol 42. issue 5. 1996-03-14. PMID:8574288. |
northern blot analyses revealed that the expression of insulin mrna markedly decreased in stz-treated rat pancreas, but a significant increase in reg mrna expression was recognized in the stz-treated rat pancreas compared with that of control rats. |
1996-03-14 |
2023-08-12 |
rat |
| H Hoshi, S Konno, M Kikuchi, Y Sendai, T Sato. Fibroblast growth factor stimulates the gene expression and production of tissue inhibitor of metalloproteinase-1 in bovine granulosa cells. In vitro cellular & developmental biology. Animal. vol 31. issue 7. 1996-02-01. PMID:8528506. |
consistent with the timp-1 production data, bfgf and afgf increased the expression of timp-1 mrna as determined by northern blot analysis, while insulin, inhibited timp-1 mrna levels. |
1996-02-01 |
2023-08-12 |
cattle |
| A Bereket, C H Lang, S L Blethen, T A Wilso. Insulin-like growth factor-binding protein-2 and insulin: studies in children with type 1 diabetes mellitus and maturity-onset diabetes of the young. The Journal of clinical endocrinology and metabolism. vol 80. issue 12. 1996-01-31. PMID:8530614. |
in the present study, serum igfbp-2 concentrations were assessed by western ligand blot and immunoblot analysis in children with newly diagnosed and untreated insulin-dependent diabetes mellitus (iddm) and maturity-onset diabetes of the young before and at various times after insulin therapy. |
1996-01-31 |
2023-08-12 |
Not clear |
| C Schalin-Jäntti, E Laurila, M Löfman, L C Groo. Determinants of insulin-stimulated skeletal muscle glycogen metabolism in man. European journal of clinical investigation. vol 25. issue 9. 1996-01-17. PMID:7498245. |
to examine factors which influence skeletal muscle glycogen synthesis in man, we related insulin sensitivity measured by euglycaemic insulin clamp in 43 healthy males to muscle glycogen synthase (gs) activity, gs protein content (western blot), glycogen concentrations and fibre composition. |
1996-01-17 |
2023-08-12 |
human |
| C Sztalryd, F B Kraeme. Regulation of hormone-sensitive lipase in streptozotocin-induced diabetic rats. Metabolism: clinical and experimental. vol 44. issue 11. 1995-12-21. PMID:7476323. |
levels of hsl mrna assessed by northern blot analysis also increased twofold (p < .01) in adipose cells isolated from streptozotocin-induced diabetic rats, and remained elevated after insulin treatment. |
1995-12-21 |
2023-08-12 |
rat |
| S Harada, R M Smith, J A Smith, N Shah, D Q Hu, L Jaret. Insulin-induced egr-1 expression in Chinese hamster ovary cells is insulin receptor and insulin receptor substrate-1 phosphorylation-independent. Evidence of an alternative signal transduction pathway. The Journal of biological chemistry. vol 270. issue 44. 1995-12-21. PMID:7592888. |
as determined by northern blot, nuclear run-on analysis, and in situ hybridization, insulin induced c-fos mrna expression, through transcription, in chohirc cells but not in choneo and choa1018k cells, consistent with previous reports. |
1995-12-21 |
2023-08-12 |
human |
| S Klaus, M Ely, D Encke, G Heldmaie. Functional assessment of white and brown adipocyte development and energy metabolism in cell culture. Dissociation of terminal differentiation and thermogenesis in brown adipocytes. Journal of cell science. vol 108 ( Pt 10). 1995-12-18. PMID:7593278. |
ucp expression in bat cultures could be detected by western blot in insulin treated, t3 treated and insulin+t3 treated cultures with highest expression in the latter. |
1995-12-18 |
2023-08-12 |
Not clear |
| C D Hardin, M J Kushmerick, T M Robert. Vascular smooth muscle glycogen metabolism studied by 13C-NMR. Journal of vascular research. vol 32. issue 5. 1995-12-13. PMID:7578797. |
during 8 h of incubation in the presence of 5 mm glucose, the content of glycogen increased from 1.5 to 8.1 mumol/g blot weight in the absence of insulin and to 11.4 mumol/g blot weight in the presence of 0.5 u/ml insulin. |
1995-12-13 |
2023-08-12 |
Not clear |
| S Babajk. Transcriptional regulation of insulin-like growth factor binding protein-1 expression by insulin and cyclic AMP. Growth regulation. vol 5. issue 2. 1995-09-07. PMID:7542954. |
western ligand blot analysis of igfbps secreted by hepg2 human hepatoma cells showed that 24 h treatment with forskolin increased igfbp-1 secretion by approximately 100%, whereas similar treatment with insulin resulted in a 50% reduction. |
1995-09-07 |
2023-08-12 |
human |