| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| D B Schranz, A M Rohilla, C Anderson, W M Wood, P Berhan. Insulin internalization in the absence of the insulin receptor tyrosine kinase domain is insufficient for mediating intracellular biological effects. Biochemical and biophysical research communications. vol 227. issue 2. 1996-12-03. PMID:8878559. |
the hir delta 343 receptors retained the ability to internalize a significant amount of surface-bound insulin at 37 degrees c. however, they were unable to mediate either the short or long-term biological effects of insulin as determined by assaying insulin-stimulated glucose uptake (assessed by 2-deoxyglucose uptake), protooncogene expression (measured by northern blot analysis of c-fos mrna) and dna synthesis (measured by 3h-thymidine incorporation). |
1996-12-03 |
2023-08-12 |
human |
| U Myrsén, B Ahrén, F Sundle. Dexamethasone-induced neuropeptide Y expression in rat islet endocrine cells. Rapid reversibility and partial prevention by insulin. Diabetes. vol 45. issue 10. 1996-11-04. PMID:8826964. |
the present study, using icc, ish, and northern blot, addressed the question whether the islet cell expression of npy induced by dex is affected by concomitant insulin (60 u/kg body wt daily for 12 days) treatment. |
1996-11-04 |
2023-08-12 |
rat |
| Y Anouar, L E Eide. Rapid and long-lasting increase in galanin mRNA levels in rat adrenal medulla following insulin-induced reflex splanchnic nerve stimulation. Neuroendocrinology. vol 62. issue 6. 1996-10-24. PMID:8751287. |
here, we examine the cellular localization and mechanism of galanin mrna upregulation in the adrenal gland after insulin administration, by northern blot and in situ histochemical hybridization analysis. |
1996-10-24 |
2023-08-12 |
rat |
| Y Shimizu, D Kielar, Y Minokoshi, T Shimaz. Noradrenaline increases glucose transport into brown adipocytes in culture by a mechanism different from that of insulin. The Biochemical journal. vol 314 ( Pt 2). 1996-08-05. PMID:8670061. |
furthermore western blot analysis with an anti-phosphotyrosine antibody revealed that, in contrast with insulin, noradrenaline apparently does not stimulate intracellular phosphorylation of tyrosine, suggesting that the noradrenaline-induced increase in dglc uptake depends on elevation of the intracellular cyclic amp level and not on the signal chain common to insulin. |
1996-08-05 |
2023-08-12 |
Not clear |
| C Reynet, C R Kahn, M R Loeke. Expression of the gene encoding glycogen phosphorylase is elevated in diabetic rat skeletal muscle and is regulated by insulin and cyclic AMP. Diabetologia. vol 39. issue 2. 1996-07-10. PMID:8635670. |
insulin stimulated the accumulation of glycogen phosphorylase mrna as determined by northern blot analysis. |
1996-07-10 |
2023-08-12 |
rat |
| T Ohno, C Ishii, N Kato, Y Ito, M Shimizu, S Tomono, K Murata, S Kawaz. Increased expression of a regenerating (reg) gene protein in neonatal rat pancreas treated with streptozotocin. Endocrine journal. vol 42. issue 5. 1996-03-14. PMID:8574288. |
northern blot analyses revealed that the expression of insulin mrna markedly decreased in stz-treated rat pancreas, but a significant increase in reg mrna expression was recognized in the stz-treated rat pancreas compared with that of control rats. |
1996-03-14 |
2023-08-12 |
rat |
| H Hoshi, S Konno, M Kikuchi, Y Sendai, T Sato. Fibroblast growth factor stimulates the gene expression and production of tissue inhibitor of metalloproteinase-1 in bovine granulosa cells. In vitro cellular & developmental biology. Animal. vol 31. issue 7. 1996-02-01. PMID:8528506. |
consistent with the timp-1 production data, bfgf and afgf increased the expression of timp-1 mrna as determined by northern blot analysis, while insulin, inhibited timp-1 mrna levels. |
1996-02-01 |
2023-08-12 |
cattle |
| A Bereket, C H Lang, S L Blethen, T A Wilso. Insulin-like growth factor-binding protein-2 and insulin: studies in children with type 1 diabetes mellitus and maturity-onset diabetes of the young. The Journal of clinical endocrinology and metabolism. vol 80. issue 12. 1996-01-31. PMID:8530614. |
in the present study, serum igfbp-2 concentrations were assessed by western ligand blot and immunoblot analysis in children with newly diagnosed and untreated insulin-dependent diabetes mellitus (iddm) and maturity-onset diabetes of the young before and at various times after insulin therapy. |
1996-01-31 |
2023-08-12 |
Not clear |
| C Schalin-Jäntti, E Laurila, M Löfman, L C Groo. Determinants of insulin-stimulated skeletal muscle glycogen metabolism in man. European journal of clinical investigation. vol 25. issue 9. 1996-01-17. PMID:7498245. |
to examine factors which influence skeletal muscle glycogen synthesis in man, we related insulin sensitivity measured by euglycaemic insulin clamp in 43 healthy males to muscle glycogen synthase (gs) activity, gs protein content (western blot), glycogen concentrations and fibre composition. |
1996-01-17 |
2023-08-12 |
human |
| C Sztalryd, F B Kraeme. Regulation of hormone-sensitive lipase in streptozotocin-induced diabetic rats. Metabolism: clinical and experimental. vol 44. issue 11. 1995-12-21. PMID:7476323. |
levels of hsl mrna assessed by northern blot analysis also increased twofold (p < .01) in adipose cells isolated from streptozotocin-induced diabetic rats, and remained elevated after insulin treatment. |
1995-12-21 |
2023-08-12 |
rat |
| S Harada, R M Smith, J A Smith, N Shah, D Q Hu, L Jaret. Insulin-induced egr-1 expression in Chinese hamster ovary cells is insulin receptor and insulin receptor substrate-1 phosphorylation-independent. Evidence of an alternative signal transduction pathway. The Journal of biological chemistry. vol 270. issue 44. 1995-12-21. PMID:7592888. |
as determined by northern blot, nuclear run-on analysis, and in situ hybridization, insulin induced c-fos mrna expression, through transcription, in chohirc cells but not in choneo and choa1018k cells, consistent with previous reports. |
1995-12-21 |
2023-08-12 |
human |
| S Klaus, M Ely, D Encke, G Heldmaie. Functional assessment of white and brown adipocyte development and energy metabolism in cell culture. Dissociation of terminal differentiation and thermogenesis in brown adipocytes. Journal of cell science. vol 108 ( Pt 10). 1995-12-18. PMID:7593278. |
ucp expression in bat cultures could be detected by western blot in insulin treated, t3 treated and insulin+t3 treated cultures with highest expression in the latter. |
1995-12-18 |
2023-08-12 |
Not clear |
| C D Hardin, M J Kushmerick, T M Robert. Vascular smooth muscle glycogen metabolism studied by 13C-NMR. Journal of vascular research. vol 32. issue 5. 1995-12-13. PMID:7578797. |
during 8 h of incubation in the presence of 5 mm glucose, the content of glycogen increased from 1.5 to 8.1 mumol/g blot weight in the absence of insulin and to 11.4 mumol/g blot weight in the presence of 0.5 u/ml insulin. |
1995-12-13 |
2023-08-12 |
Not clear |
| S Babajk. Transcriptional regulation of insulin-like growth factor binding protein-1 expression by insulin and cyclic AMP. Growth regulation. vol 5. issue 2. 1995-09-07. PMID:7542954. |
western ligand blot analysis of igfbps secreted by hepg2 human hepatoma cells showed that 24 h treatment with forskolin increased igfbp-1 secretion by approximately 100%, whereas similar treatment with insulin resulted in a 50% reduction. |
1995-09-07 |
2023-08-12 |
human |
| Y S Guo, C M Townsend, G F Jin, R D Beauchamp, J C Thompso. Differential regulation by TGF-beta 1 and insulin of insulin-like growth factor binding protein-2 in IEC-6 cells. The American journal of physiology. vol 268. issue 6 Pt 1. 1995-08-15. PMID:7541944. |
utilizing western ligand blot analysis, we found that tgf-beta 1 at concentrations of 0.5, 1.0, and 2 ng/ml significantly increased levels of 32-kda igfbp in the conditioned medium (cm) of iec-6 cells in a dose-dependent fashion and that low doses of insulin (1.0 and 5.0 microgram/ml) also increased igfbp levels in the cm of iec-6 cells, but a high dose of insulin (10 micrograms/ml) depressed igfbp release in the cm. |
1995-08-15 |
2023-08-12 |
Not clear |
| N Welsh, K Bendtzen, M Wels. Expression of an insulin/interleukin-1 receptor antagonist hybrid gene in insulin-producing cell lines (HIT-T15 and NIT-1) confers resistance against interleukin-1-induced nitric oxide production. The Journal of clinical investigation. vol 95. issue 4. 1995-05-09. PMID:7706480. |
one of the geneticin-selected clones, hitra2, expressed a 1.4-kb mrna, which hybridized both to insulin and il-1ra-cdna in northern blot analysis. |
1995-05-09 |
2023-08-12 |
human |
| P J Miettinen, T Otonkoski, R Voutilaine. Insulin-like growth factor-II and transforming growth factor-alpha in developing human fetal pancreatic islets. The Journal of endocrinology. vol 138. issue 1. 1995-03-10. PMID:7852883. |
northern blot analysis revealed an abundant expression of igf-ii, insulin and tgf-alpha mrnas in the intact pancreas and the cultured iccs. |
1995-03-10 |
2023-08-12 |
human |
| I Conget, Y Sarri, A Novials, R Casamitjana, M Vives, R Gomi. Functional properties of isolated human pancreatic islets beneficial effects of culture and exposure to high glucose concentrations. Diabete & metabolisme. vol 20. issue 2. 1995-02-02. PMID:7805958. |
northern blot analysis was performed to measure insulin mrna levels. |
1995-02-02 |
2023-08-12 |
human |
| B Tang, S Guller, E Gurpid. Mechanisms involved in the decidualization of human endometrial stromal cells. Acta Europaea fertilitatis. vol 24. issue 5. 1995-01-05. PMID:7985467. |
exposure of stromal cell cultures to db-camp, 8-br-camp or forskolin in rpmi 1640 medium containing 2% ct-fcs and 0.1 u/ml insulin induces the expression of prolactin (prl), evident from 1) its secretion to the culture medium, measured by radioimmunoassay and by western blot analysis, 2) the incorporation of 35s-methionine in a protein precipitated with a prl antibody and co-migrating with authentic prl during electrophoresis, and 3) the synthesis of prl mrna determined by northern blot analysis. |
1995-01-05 |
2023-08-12 |
human |
| F S Celi, K Tanner, A K Roth, A E Roth, A R Shuldine. The two nonallelic Xenopus insulin genes are expressed coordinately in the adult pancreas. General and comparative endocrinology. vol 95. issue 2. 1994-12-28. PMID:7525401. |
insulin i and ii mrnas were quantitated by slot blot hybridization with specific probes and were expressed as the number of copies (x 10(8)) per 5 micrograms total rna +/- sem. |
1994-12-28 |
2023-08-12 |
xenopus_laevis |