| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Soheila Marandi, Nadine De Keyser, Catherine Stilmant, Alain Saliez, Etienne Marc Sokal, Yves Guiot, Jean-Paul But. Ontogeny of MAP kinases in rat small intestine: premature stimulation by insulin of BBM hydrolases is regulated by ERKs but not by p-38 MAP kinase. Pediatric research. vol 52. issue 2. 2003-01-13. PMID:12149494. |
therefore, we have analyzed the mucosal distribution, ontogeny, and responses to insulin and to inhibitors of p44, p42, and p38 map kinases in immature and mature enterocytes using western blot analysis and autoradiography after immunoprecipitation, immunohistochemistry, and in vitro phosphorylation assays. |
2003-01-13 |
2023-08-12 |
rat |
| Sakeneh Zraika, Marjorie Dunlop, Joseph Proietto, Sofianos Andrikopoulo. The hexosamine biosynthesis pathway regulates insulin secretion via protein glycosylation in mouse islets. Archives of biochemistry and biophysics. vol 405. issue 2. 2002-11-08. PMID:12220542. |
western blot analysis revealed that decreased islet protein glycosylation paralleled the decrease in insulin secretion following inhibition of the pathway. |
2002-11-08 |
2023-08-12 |
mouse |
| Richard C Ho, Kevin P Davy, Matthew S Hickey, Scott A Summers, Christopher L Melb. Behavioral, metabolic, and molecular correlates of lower insulin sensitivity in Mexican-Americans. American journal of physiology. Endocrinology and metabolism. vol 283. issue 4. 2002-10-10. PMID:12217898. |
we determined whether lower insulin sensitivity persists in young, nonobese, nondiabetic mexican-american [ma; n = 13, 27.0 +/- 2.0 yr, body mass index (bmi) 23.0 +/- 0.7] compared with non-hispanic white (nhw; n = 13, 24.8 +/- 1.5 yr, bmi 22.8 +/- 0.6) males and females after accounting for cardiorespiratory fitness (maximal o(2) uptake), abdominal fat distribution (computed tomography scans), dietary intake (4-day records), and skeletal muscle insulin-signaling protein abundance from muscle biopsies (western blot analysis). |
2002-10-10 |
2023-08-12 |
Not clear |
| W S Zawalich, G J Tesz, K C Zawalic. Inhibitors of phosphatidylinositol 3-kinase amplify insulin release from islets of lean but not obese mice. The Journal of endocrinology. vol 174. issue 2. 2002-10-08. PMID:12176663. |
islets were collagenase isolated and perifused or subjected to western blot analyses and probed for insulin receptor-signaling components. |
2002-10-08 |
2023-08-12 |
mouse |
| Joe M Chehade, Michael J Haas, Arshag D Mooradia. Diabetes-related changes in rat cerebral occludin and zonula occludens-1 (ZO-1) expression. Neurochemical research. vol 27. issue 3. 2002-10-04. PMID:11958524. |
to test this hypothesis, western and northern blot analysis were carried out to measure the steady-state level of occludin and zonula occludens-one (zo-1) proteins and mrna levels in cerebral tissue of streptozotocin-induced diabetic rats and the results were compared to insulin treated diabetic rats and vehicle injected control rats. |
2002-10-04 |
2023-08-12 |
rat |
| Hongxiang Hui, Run Yu, Corinne Bousquet, Riccardo Perfett. Transfection of pancreatic-derived beta-cells with a minigene encoding for human glucagon-like peptide-1 regulates glucose-dependent insulin synthesis and secretion. Endocrinology. vol 143. issue 9. 2002-09-23. PMID:12193567. |
detection of the counterpart proteins (insulin and glp-1) in the culture medium paralleled the observation derived from the northern blot analysis. |
2002-09-23 |
2023-08-12 |
mouse |
| Hamid Sadeghi, Søren Bregenholt, Dale Wegmann, Jacob S Petersen, Jan Holmgren, Michael Leben. Genetic fusion of human insulin B-chain to the B-subunit of cholera toxin enhances in vitro antigen presentation and induction of bystander suppression in vivo. Immunology. vol 106. issue 2. 2002-07-15. PMID:12047753. |
gm1 enzyme-linked immunosorbent assay (elisa), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and western blot analyses showed that the protein construct in which insb was fused to the c-terminus of a ctb monomer (ci) assembled into structures that both bound to the receptor gm1 ganglioside and reacted with monoclonal antibodies to ctb and insulin. |
2002-07-15 |
2023-08-12 |
mouse |
| Víctor M Rodríguez, Catalina Picó, María P Portillo, M Teresa Macarulla, Andreu Palo. Dietary fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding. The British journal of nutrition. vol 87. issue 5. 2002-06-19. PMID:12010582. |
insulin and leptin were determined by elisa and ob mrna by northern blot. |
2002-06-19 |
2023-08-12 |
rat |
| H Hori, Y J Gu, N Nagata, A N Balamurugan, A Satake, Y Morimoto, W J Wang, Y Misawa, Y Nozawa, T Nembai, M Miyamoto, M Nozawa, K Inou. Isolation, culture, and characterization of endocrine cells from 6-month-old porcine pancreas. Cell transplantation. vol 10. issue 4-5. 2002-04-09. PMID:11549072. |
the suspension cells were tested for insulin and glucagon staining by western blot analysis. |
2002-04-09 |
2023-08-12 |
mouse |
| P Ostlund, H Lindegren, C Pettersson, K Bedec. Altered insulin receptor processing and function in scrapie-infected neuroblastoma cell lines. Brain research. Molecular brain research. vol 97. issue 2. 2002-03-21. PMID:11750072. |
in addition to these differences in ir properties, the basal extracellular signal regulated kinase-2 (erk2) phosphorylation detected by western blot, was significantly elevated and the insulin stimulated erk2 phosphorylation was subsequently decreased in scn2a. |
2002-03-21 |
2023-08-12 |
Not clear |
| Mohammed Taouis, Carine Dagou, Céline Ster, Georges Durand, Michèle Pinault, Jacques Delaru. N-3 polyunsaturated fatty acids prevent the defect of insulin receptor signaling in muscle. American journal of physiology. Endocrinology and metabolism. vol 282. issue 3. 2002-03-19. PMID:11832371. |
in contrast, in leg muscle, ir density, as determined by western blotting, was not affected, whereas ir and irs-1 tyrosine phosphorylation in response to insulin treatment was restored in animals fed with n-3 pufa to normal; in n-6 pufa, the phosphorylation was depressed, as evidenced by western blot analysis using specific antibodies. |
2002-03-19 |
2023-08-12 |
rat |
| D Yin, S D Clarke, T D Etherto. Transcriptional regulation of fatty acid synthase gene by somatotropin in 3T3-F442A adipocytes. Journal of animal science. vol 79. issue 9. 2002-03-13. PMID:11583420. |
western blot analyses of nuclear extracts from cells treated with insulin (10 ng/ml) or insulin (10 ng/ml) plus bst (100 ng/ml) for 48 h demonstrated that the abundance of usf1 was not affected by insulin or st. |
2002-03-13 |
2023-08-12 |
human |
| E Zhukova, A Afshar, J Ko, P Popper, T Pham, C Sternini, J H Wals. Expression of the human insulin gene in the gastric G cells of transgenic mice. Transgenic research. vol 10. issue 4. 2002-02-21. PMID:11592712. |
northern blot analysis, in situ hybridization, and immunohistochemistry demonstrated expression of the human insulin gene specifically in antral g cells. |
2002-02-21 |
2023-08-12 |
mouse |
| E Zhukova, A Afshar, J Ko, P Popper, T Pham, C Sternini, J H Wals. Expression of the human insulin gene in the gastric G cells of transgenic mice. Transgenic research. vol 10. issue 4. 2002-02-21. PMID:11592712. |
northern blot analysis demonstrated that the shortest of the insulin mrna three forms is predominantly expressed in stomach tissue. |
2002-02-21 |
2023-08-12 |
mouse |
| James A Rillema, Charles H Williams, Jerome Moulden, Kish L Golde. Effect of insulin on iodide uptake in mouse mammary gland explants. Experimental biology and medicine (Maywood, N.J.). vol 227. issue 1. 2002-01-30. PMID:11788781. |
the optimal cortisol concentration for a maximum stimulation of iodide uptake was 10(-7)m. in a quantitative western blot analysis employing an antibody to the sodium-iodide symporter, insulin stimulated an upregulation of the transporter protein after a 4-, 8-, or 20-hr treatment with insulin. |
2002-01-30 |
2023-08-12 |
mouse |
| V A Skeberdis, J Lan, X Zheng, R S Zukin, M V Bennet. Insulin promotes rapid delivery of N-methyl-D- aspartate receptors to the cell surface by exocytosis. Proceedings of the National Academy of Sciences of the United States of America. vol 98. issue 6. 2001-12-04. PMID:11248117. |
the present study shows that insulin induced (i) an increase in channel number times open probability (np(o)) in outside-out patches excised from xenopus oocytes, with no change in mean open time, unitary conductance, or reversal potential, indicating an increase in n and/or p(o); (ii) an increase in charge transfer during block of nmda-elicited currents by the open channel blocker mk-801, indicating increased number of functional nmdars in the cell membrane with no change in p(o); and (iii) increased nr1 surface expression, as indicated by western blot analysis of surface proteins. |
2001-12-04 |
2023-08-12 |
xenopus_laevis |
| A V Chibalin, M V Kovalenko, J W Ryder, E Féraille, H Wallberg-Henriksson, J R Zierat. Insulin- and glucose-induced phosphorylation of the Na(+),K(+)-adenosine triphosphatase alpha-subunits in rat skeletal muscle. Endocrinology. vol 142. issue 8. 2001-08-16. PMID:11459793. |
immunoprecipitation with antiphosphotyrosine antibody and subsequent western blot analysis with anti-alpha(1)- and -alpha(2)-subunit antibodies revealed that both alpha(1)- and alpha(2)-subunit isoforms underwent phosphorylation on tyrosine residues in response to insulin, although with different time course and magnitude. |
2001-08-16 |
2023-08-12 |
rat |
| X Han, B E Tuc. Cloning and characterization of porcine insulin gene. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. vol 129. issue 1. 2001-08-09. PMID:11337252. |
northern blot analysis of isolated adult porcine islets demonstrated an increase in steady-state insulin mrna levels in response to high concentrations of glucose. |
2001-08-09 |
2023-08-12 |
Not clear |
| N Chang, J M Uribe, S J Keely, S Calandrella, K E Barret. Insulin and IGF-I inhibit calcium-dependent chloride secretion by T84 human colonic epithelial cells. American journal of physiology. Gastrointestinal and liver physiology. vol 281. issue 1. 2001-07-19. PMID:11408264. |
western blot analysis showed that both insulin and igf-i recruited the 85-kda regulatory and 110-kda catalytic subunits of pi 3-kinase to anti-phosphotyrosine immunoprecipitates. |
2001-07-19 |
2023-08-12 |
human |
| S Morimoto, M A Cerbón, A Alvarez-Alvarez, G Romero-Navarro, V Díaz-Sánche. Insulin gene expression pattern in rat pancreas during the estrous cycle. Life sciences. vol 68. issue 26. 2001-07-05. PMID:11411797. |
a northern blot analysis was performed to assess insulin gene expression in pancreatic tissue. |
2001-07-05 |
2023-08-12 |
rat |