| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| H L Kaung, C Y Wang, W W Che. Development of rat pancreatic islet cells in vitro. Microscopy research and technique. vol 43. issue 4. 1999-02-19. PMID:9849971. |
northern blot analyses also showed corresponding changes of insulin expression in these cells at different passages. |
1999-02-19 |
2023-08-12 |
rat |
| H L Kaung, C Y Wang, W W Che. Development of rat pancreatic islet cells in vitro. Microscopy research and technique. vol 43. issue 4. 1999-02-19. PMID:9849971. |
by passage 35, no insulin or glucagon expression was detectable by northern blot analysis or immunocytochemistry. |
1999-02-19 |
2023-08-12 |
rat |
| T A Kupriyanova, K V Kandro. Akt-2 binds to Glut4-containing vesicles and phosphorylates their component proteins in response to insulin. The Journal of biological chemistry. vol 274. issue 3. 1999-02-11. PMID:9880520. |
by means of monoq chromatography and western blot analysis, vesicle-associated protein kinase is identified as akt-2, a lipid-binding protein kinase involved in insulin signaling. |
1999-02-11 |
2023-08-12 |
rat |
| R Henningsson, P Alm, P Ekström, I Lundquis. Heme oxygenase and carbon monoxide: regulatory roles in islet hormone release: a biochemical, immunohistochemical, and confocal microscopic study. Diabetes. vol 48. issue 1. 1999-02-10. PMID:9892224. |
western blot showed that islet tissue expressed ho-2, and confocal microscopy revealed that ho-2 resided in insulin, glucagon, somatostatin, and pancreatic polypeptide cells. |
1999-02-10 |
2023-08-12 |
mouse |
| R Schechter, T Yanovitch, M Abboud, G Johnson, J Gaskin. Effects of brain endogenous insulin on neurofilament and MAPK in fetal rat neuron cell cultures. Brain research. vol 808. issue 2. 1998-12-11. PMID:9767173. |
western blot analysis showed that the basal level of mitogen activated protein kinase (mapk) phosphorylation was inhibited by the treatment of the ncc with isoproterenol or trypsin, but was significantly increased by treatment with exogenous insulin, demonstrating that brain endogenous insulin phosphorylated mapk (p<0.05). |
1998-12-11 |
2023-08-12 |
rat |
| D Lu, H Tamemoto, H Shibata, I Saito, T Takeuch. Regulatable production of insulin from primary-cultured hepatocytes: insulin production is up-regulated by glucagon and cAMP and down-regulated by insulin. Gene therapy. vol 5. issue 7. 1998-11-30. PMID:9813659. |
addition of exogenous insulin to the culture decreased insulin mrna expression remarkably on northern blot. |
1998-11-30 |
2023-08-12 |
Not clear |
| H M Peng, M J Coo. Regulation of rabbit cytochrome P450 2E1 expression in HepG2 cells by insulin and thyroid hormone. Molecular pharmacology. vol 54. issue 4. 1998-10-30. PMID:9765518. |
western blot analysis showed that 1 microm insulin decreased the cyp2e1 protein level in a dose- and time-dependent manner, whereas 1 microm t3 increased the level 2-fold in 1 day and 8-fold in 5 days. |
1998-10-30 |
2023-08-12 |
rabbit |
| G G Xu, P L Rothenber. Insulin receptor signaling in the beta-cell influences insulin gene expression and insulin content: evidence for autocrine beta-cell regulation. Diabetes. vol 47. issue 8. 1998-09-09. PMID:9703324. |
steady-state insulin mrna levels were elevated twofold in the beta-cells overexpressing the wild-type ir and unchanged in the beta-cells expressing the kinase-inactive receptor, as determined by northern blot analysis. |
1998-09-09 |
2023-08-12 |
Not clear |
| P Fragner, J Quette, S Aratan-Spir. Thyroid status and the regulation of thyrotropin-releasing hormone synthesis in rat pancreatic islets: comparison with insulin regulation. Biochemical and biophysical research communications. vol 247. issue 3. 1998-07-31. PMID:9647733. |
likewise, the northern blot analysis revealed that the steady-state concentrations of trh mrna increased (4x) while those of insulin remain unchanged. |
1998-07-31 |
2023-08-12 |
rat |
| R L Hopfner, R V Hasnadka, J R McNeill, T W Wilson, V Gopalakrishna. Differential regulation of endothelin-1 action by insulin and vanadate in vascular smooth muscle. Journal of cardiovascular pharmacology. vol 31 Suppl 1. 1998-07-17. PMID:9595436. |
northern blot analysis revealed that insulin upregulated only eta mrna levels, and sov enhanced both eta and etb mrna levels. |
1998-07-17 |
2023-08-12 |
rat |
| R L Hopfner, R V Hasnadka, T W Wilson, J R McNeill, V Gopalakrishna. Insulin increases endothelin-1-evoked intracellular free calcium responses by increased ET(A) receptor expression in rat aortic smooth muscle cells. Diabetes. vol 47. issue 6. 1998-06-16. PMID:9604872. |
northern blot analysis revealed an increase in et(a) mrna levels after insulin pretreatment for 20 h, an effect that was blocked by genistein, actinomycin d, and cycloheximide. |
1998-06-16 |
2023-08-12 |
rat |
| F Koumanov, J Yang, A E Jones, Y Hatanaka, G D Holma. Cell-surface biotinylation of GLUT4 using bis-mannose photolabels. The Biochemical journal. vol 330 ( Pt 3). 1998-05-21. PMID:9494087. |
insulin treatment of adipocytes increased the levels of signals from surface biotinylated glut4 by approximately 10-fold or approximately 20-fold, respectively, when the electrochemiluminescent or the western blot detection methods were used and these signals were blocked by cytochalasin b. |
1998-05-21 |
2023-08-12 |
rat |
| P Vorwerk, Y Yamanaka, A Spagnoli, Y Oh, R G Rosenfel. Insulin and IGF binding by IGFBP-3 fragments derived from proteolysis, baculovirus expression and normal human urine. The Journal of clinical endocrinology and metabolism. vol 83. issue 4. 1998-04-21. PMID:9543173. |
the fragments resulting from plasmin digestion, as well as the baculovirus-expressed recombinant human igfbp-3(1-97), retain weak igf binding and show specific insulin binding on cross-linking and western ligand blot. |
1998-04-21 |
2023-08-12 |
human |
| U G Das, H F Sadiq, M J Soares, W W Hay, S U Devaska. Time-dependent physiological regulation of rodent and ovine placental glucose transporter (GLUT-1) protein. The American journal of physiology. vol 274. issue 2. 1998-03-17. PMID:9486290. |
to examine the in vivo and in vitro time-dependent effects of glucose on placental glucose transporter (glut-1) protein levels, we employed western blot analysis using placenta from the short-term streptozotocin-induced diabetic pregnancy (stz-d), uterine artery ligation-intrauterine growth restriction (iugr) rat models, pregnant sheep exposed to chronic maternal glucose and insulin infusions, and the hrp.1 rat trophoblastic cell line exposed to differing concentrations of glucose. |
1998-03-17 |
2023-08-12 |
rat |
| J W Moore, M A Maher, W J Banz, M B Zeme. Chromium picolinate modulates rat vascular smooth muscle cell intracellular calcium metabolism. The Journal of nutrition. vol 128. issue 2. 1998-03-05. PMID:9446840. |
cultured vsmc from rats were grown from passage to confluence in the presence or absence of 1 micromol/l crpic, maintained in a quiescent medium for 24 h and incubated with or without insulin (10(-8) mol/l) for the final 2 h. cells were then harvested and rna and protein extracted for northern and western blot analysis, respectively. |
1998-03-05 |
2023-08-12 |
human |
| L Gros, L Montoliu, E Riu, L Lebrigand, F Bosc. Regulated production of mature insulin by non-beta-cells. Human gene therapy. vol 8. issue 18. 1998-02-25. PMID:9449378. |
the ftoinsm cells expressed high levels of insulin mrna and protein after northern blot or immunocytochemical analysis. |
1998-02-25 |
2023-08-12 |
human |
| G L Niermann, G C Buehrin. Hormone regulation of bovine leukemia virus via the long terminal repeat. Virology. vol 239. issue 2. 1998-02-12. PMID:9434716. |
only dexamethasone, in combination with insulin or insulin/prolactin, consistently stimulated blv expression, as measured by reverse transcriptase activity, rna blot hybridization (northern blots), and cat (chloramphenicol acetyltransferase) reporter assays of cell lines transiently or stably transfected with the blv ltr. |
1998-02-12 |
2023-08-12 |
mouse |
| L Q Dong, H Du, S G Porter, L F Kolakowski, A V Lee, L J Mandarino, J Fan, D Yee, F Liu, J Mandarin. Cloning, chromosome localization, expression, and characterization of an Src homology 2 and pleckstrin homology domain-containing insulin receptor binding protein hGrb10gamma. The Journal of biological chemistry. vol 272. issue 46. 1997-12-11. PMID:9360986. |
rnase protection assays and western blot analysis showed that hgrb10alpha and hgrb10gamma are differentially expressed in insulin target cells including skeletal muscle, liver, and adipocyte cells. |
1997-12-11 |
2023-08-12 |
human |
| Y Kajimoto, H Watada, T a Matsuoka, H Kaneto, Y Fujitani, J Miyazaki, Y Yamasak. Suppression of transcription factor PDX-1/IPF1/STF-1/IDX-1 causes no decrease in insulin mRNA in MIN6 cells. The Journal of clinical investigation. vol 100. issue 7. 1997-10-29. PMID:9312185. |
despite such suppression of pdx-1, northern blot analysis revealed no decrease in the amount of insulin mrna in the min6 cells. |
1997-10-29 |
2023-08-12 |
Not clear |
| H Wang, Z Xie, R E Scot. Induction of AP-1 activity associated with c-Jun and JunB is required for mitogenesis induced by insulin and vanadate in SV40-transformed 3T3T cells. Molecular and cellular biochemistry. vol 168. issue 1-2. 1997-05-27. PMID:9062890. |
gel supershift assays and western blot analysis using specific antibodies demonstrate that the increased ap-1 binding activity induced by insulin and vanadate in csv3-1 cells is primarily contributed by an increase in the expression of c-jun and junb protein levels. |
1997-05-27 |
2023-08-12 |
Not clear |