| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| H M Peng, M J Coo. Regulation of rabbit cytochrome P450 2E1 expression in HepG2 cells by insulin and thyroid hormone. Molecular pharmacology. vol 54. issue 4. 1998-10-30. PMID:9765518. |
western blot analysis showed that 1 microm insulin decreased the cyp2e1 protein level in a dose- and time-dependent manner, whereas 1 microm t3 increased the level 2-fold in 1 day and 8-fold in 5 days. |
1998-10-30 |
2023-08-12 |
rabbit |
| G G Xu, P L Rothenber. Insulin receptor signaling in the beta-cell influences insulin gene expression and insulin content: evidence for autocrine beta-cell regulation. Diabetes. vol 47. issue 8. 1998-09-09. PMID:9703324. |
steady-state insulin mrna levels were elevated twofold in the beta-cells overexpressing the wild-type ir and unchanged in the beta-cells expressing the kinase-inactive receptor, as determined by northern blot analysis. |
1998-09-09 |
2023-08-12 |
Not clear |
| P Fragner, J Quette, S Aratan-Spir. Thyroid status and the regulation of thyrotropin-releasing hormone synthesis in rat pancreatic islets: comparison with insulin regulation. Biochemical and biophysical research communications. vol 247. issue 3. 1998-07-31. PMID:9647733. |
likewise, the northern blot analysis revealed that the steady-state concentrations of trh mrna increased (4x) while those of insulin remain unchanged. |
1998-07-31 |
2023-08-12 |
rat |
| R L Hopfner, R V Hasnadka, J R McNeill, T W Wilson, V Gopalakrishna. Differential regulation of endothelin-1 action by insulin and vanadate in vascular smooth muscle. Journal of cardiovascular pharmacology. vol 31 Suppl 1. 1998-07-17. PMID:9595436. |
northern blot analysis revealed that insulin upregulated only eta mrna levels, and sov enhanced both eta and etb mrna levels. |
1998-07-17 |
2023-08-12 |
rat |
| R L Hopfner, R V Hasnadka, T W Wilson, J R McNeill, V Gopalakrishna. Insulin increases endothelin-1-evoked intracellular free calcium responses by increased ET(A) receptor expression in rat aortic smooth muscle cells. Diabetes. vol 47. issue 6. 1998-06-16. PMID:9604872. |
northern blot analysis revealed an increase in et(a) mrna levels after insulin pretreatment for 20 h, an effect that was blocked by genistein, actinomycin d, and cycloheximide. |
1998-06-16 |
2023-08-12 |
rat |
| F Koumanov, J Yang, A E Jones, Y Hatanaka, G D Holma. Cell-surface biotinylation of GLUT4 using bis-mannose photolabels. The Biochemical journal. vol 330 ( Pt 3). 1998-05-21. PMID:9494087. |
insulin treatment of adipocytes increased the levels of signals from surface biotinylated glut4 by approximately 10-fold or approximately 20-fold, respectively, when the electrochemiluminescent or the western blot detection methods were used and these signals were blocked by cytochalasin b. |
1998-05-21 |
2023-08-12 |
rat |
| P Vorwerk, Y Yamanaka, A Spagnoli, Y Oh, R G Rosenfel. Insulin and IGF binding by IGFBP-3 fragments derived from proteolysis, baculovirus expression and normal human urine. The Journal of clinical endocrinology and metabolism. vol 83. issue 4. 1998-04-21. PMID:9543173. |
the fragments resulting from plasmin digestion, as well as the baculovirus-expressed recombinant human igfbp-3(1-97), retain weak igf binding and show specific insulin binding on cross-linking and western ligand blot. |
1998-04-21 |
2023-08-12 |
human |
| U G Das, H F Sadiq, M J Soares, W W Hay, S U Devaska. Time-dependent physiological regulation of rodent and ovine placental glucose transporter (GLUT-1) protein. The American journal of physiology. vol 274. issue 2. 1998-03-17. PMID:9486290. |
to examine the in vivo and in vitro time-dependent effects of glucose on placental glucose transporter (glut-1) protein levels, we employed western blot analysis using placenta from the short-term streptozotocin-induced diabetic pregnancy (stz-d), uterine artery ligation-intrauterine growth restriction (iugr) rat models, pregnant sheep exposed to chronic maternal glucose and insulin infusions, and the hrp.1 rat trophoblastic cell line exposed to differing concentrations of glucose. |
1998-03-17 |
2023-08-12 |
rat |
| J W Moore, M A Maher, W J Banz, M B Zeme. Chromium picolinate modulates rat vascular smooth muscle cell intracellular calcium metabolism. The Journal of nutrition. vol 128. issue 2. 1998-03-05. PMID:9446840. |
cultured vsmc from rats were grown from passage to confluence in the presence or absence of 1 micromol/l crpic, maintained in a quiescent medium for 24 h and incubated with or without insulin (10(-8) mol/l) for the final 2 h. cells were then harvested and rna and protein extracted for northern and western blot analysis, respectively. |
1998-03-05 |
2023-08-12 |
human |
| L Gros, L Montoliu, E Riu, L Lebrigand, F Bosc. Regulated production of mature insulin by non-beta-cells. Human gene therapy. vol 8. issue 18. 1998-02-25. PMID:9449378. |
the ftoinsm cells expressed high levels of insulin mrna and protein after northern blot or immunocytochemical analysis. |
1998-02-25 |
2023-08-12 |
human |
| G L Niermann, G C Buehrin. Hormone regulation of bovine leukemia virus via the long terminal repeat. Virology. vol 239. issue 2. 1998-02-12. PMID:9434716. |
only dexamethasone, in combination with insulin or insulin/prolactin, consistently stimulated blv expression, as measured by reverse transcriptase activity, rna blot hybridization (northern blots), and cat (chloramphenicol acetyltransferase) reporter assays of cell lines transiently or stably transfected with the blv ltr. |
1998-02-12 |
2023-08-12 |
mouse |
| L Q Dong, H Du, S G Porter, L F Kolakowski, A V Lee, L J Mandarino, J Fan, D Yee, F Liu, J Mandarin. Cloning, chromosome localization, expression, and characterization of an Src homology 2 and pleckstrin homology domain-containing insulin receptor binding protein hGrb10gamma. The Journal of biological chemistry. vol 272. issue 46. 1997-12-11. PMID:9360986. |
rnase protection assays and western blot analysis showed that hgrb10alpha and hgrb10gamma are differentially expressed in insulin target cells including skeletal muscle, liver, and adipocyte cells. |
1997-12-11 |
2023-08-12 |
human |
| Y Kajimoto, H Watada, T a Matsuoka, H Kaneto, Y Fujitani, J Miyazaki, Y Yamasak. Suppression of transcription factor PDX-1/IPF1/STF-1/IDX-1 causes no decrease in insulin mRNA in MIN6 cells. The Journal of clinical investigation. vol 100. issue 7. 1997-10-29. PMID:9312185. |
despite such suppression of pdx-1, northern blot analysis revealed no decrease in the amount of insulin mrna in the min6 cells. |
1997-10-29 |
2023-08-12 |
Not clear |
| H Wang, Z Xie, R E Scot. Induction of AP-1 activity associated with c-Jun and JunB is required for mitogenesis induced by insulin and vanadate in SV40-transformed 3T3T cells. Molecular and cellular biochemistry. vol 168. issue 1-2. 1997-05-27. PMID:9062890. |
gel supershift assays and western blot analysis using specific antibodies demonstrate that the increased ap-1 binding activity induced by insulin and vanadate in csv3-1 cells is primarily contributed by an increase in the expression of c-jun and junb protein levels. |
1997-05-27 |
2023-08-12 |
Not clear |
| B F Lindgren, I Odar-Cederlöf, F Ericsson, K Brisma. Decreased bioavailability of insulin-like growth factor-I, a cause of catabolism in hemodialysis patients? Growth regulation. vol 6. issue 3. 1997-05-16. PMID:8894646. |
in esrd patients predialysis mean values of insulin, igf-i sd-score, igfbp-1 and igfbp-2 were increased, while the mean densitrometric values of the igfbp-3 bands on western ligand blot were either normal or reduced. |
1997-05-16 |
2023-08-12 |
Not clear |
| J F Wang, G P Becks, D J Hil. Degradation of IGF-binding protein-3 by proteases in cultured FRTL-5 rat thyroid cells. The Journal of endocrinology. vol 152. issue 2. 1997-04-02. PMID:9071984. |
when frtl-5 rat thyroid cells were cultured in 6h medium (modified f-12m medium containing tsh, insulin, hydrocortisone, somatostatin, transferrin, and glycyl-histidyl-lysine) containing 5% calf serum, both 44-46 and 31 kda igfbps were found in conditioned medium by ligand blot analysis using 125i-labelled igf-ii. |
1997-04-02 |
2023-08-12 |
human |
| I Vietor, M Rusnak, E Viskupic, P Blazicek, E L Sabban, R Kvetnansk. Glucoprivation by insulin leads to trans-synaptic increase in rat adrenal tyrosine hydroxylase mRNA levels. European journal of pharmacology. vol 313. issue 1-2. 1997-02-27. PMID:8905338. |
western blot analysis showed that although a single insulin treatment did not affect tyrosine hydroxylase protein levels, a significant increase was observed following the seventh administration. |
1997-02-27 |
2023-08-12 |
rat |
| D Lu, H Hoshino, T Takeuch. Regulatable production of mature insulin from a hepatocyte cell line: insulin production is up-regulated by cAMP and glucocorticoids, and down-regulated by insulin. FEBS letters. vol 399. issue 1-2. 1997-01-31. PMID:8980115. |
addition of exogenous insulin to the culture medium significantly decreased insulin mrna expression on northern blot. |
1997-01-31 |
2023-08-12 |
rat |
| S Harada, R M Smith, J A Smith, M F White, L Jaret. Insulin-induced egr-1 and c-fos expression in 32D cells requires insulin receptor, Shc, and mitogen-activated protein kinase, but not insulin receptor substrate-1 and phosphatidylinositol 3-kinase activation. The Journal of biological chemistry. vol 271. issue 47. 1997-01-13. PMID:8939974. |
insulin (17 nm) increased egr-1 and c-fos expression in 32d/ir and 32d/ir+irs cells, but not in parental cells or 32d/irs cells, as determined by northern blot analysis. |
1997-01-13 |
2023-08-12 |
Not clear |
| S Harada, R M Smith, J A Smith, M F White, L Jaret. Insulin-induced egr-1 and c-fos expression in 32D cells requires insulin receptor, Shc, and mitogen-activated protein kinase, but not insulin receptor substrate-1 and phosphatidylinositol 3-kinase activation. The Journal of biological chemistry. vol 271. issue 47. 1997-01-13. PMID:8939974. |
insulin treatment (5 min at 37 degrees c) markedly increased tyrosine phosphorylation of several proteins, including the insulin receptor, irs-1, and shc, in 32d/ir+irs cells as determined by immunoprecipitation and western blot analysis with anti-phosphotyrosine antibody. |
1997-01-13 |
2023-08-12 |
Not clear |