| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| A Katsurada, N Iritani, H Fukuda, Y Matsumura, T Noguchi, T Tanak. Effects of insulin and fructose on transcriptional and post-transcriptional regulation of malic enzyme synthesis in diabetic rat liver. Biochimica et biophysica acta. vol 1004. issue 1. 1989-08-25. PMID:2663078. |
in northern blot analysis, we demonstrated that after insulin treatment, the nuclear mrna of malic enzyme tended to increase more rapidly than the total cellular mrna. |
1989-08-25 |
2023-08-11 |
rat |
| P S Walker, T Ramlal, J A Donovan, T P Doering, A Sandra, A Klip, J E Pessi. Insulin and glucose-dependent regulation of the glucose transport system in the rat L6 skeletal muscle cell line. The Journal of biological chemistry. vol 264. issue 11. 1989-05-18. PMID:2649505. |
similarly, northern blot analysis of total rna isolated from glucose-deprived, insulin, and glucose-deprived plus insulin-treated cells resulted in a 4-, 3-, and 9-fold induction of glucose transporter mrna, respectively. |
1989-05-18 |
2023-08-11 |
rat |
| C Neumer, R Brandt, U Lendeckel, H Zühlke, I Rjasanowski, D Michaeli. Restriction-fragment-length-polymorphisms close to the human insulin gene on chromosome 11 and their possible relation to diabetes mellitus in a GDR population. Biomedica biochimica acta. vol 47. issue 4-5. 1989-04-25. PMID:2907291. |
a polymorphic dna sequence flanking the 5'-region of the human insulin gene was studied by means of southern blot hybridization techniques in 92 diabetic and non-diabetic individuals in order to investigate the possible relation of their allelic variants to certain types of diabetes. |
1989-04-25 |
2023-08-11 |
human |
| S Ito, T Suzuki, S Isemura, K Sanada, H Anaguchi, H Shimizu, T Maruyama, A Shibat. 'Salivary peptide P-C' of human pancreatic B-cells shares only partly immunoreactivity with salivary peptide P-C indicating a new B-cell protein which is different from insulin. Acta endocrinologica. vol 120. issue 1. 1989-02-16. PMID:2643248. |
the result showed that pancreatic p-c substance was a substance which shares the common antigenic site with the 38-44 amino acid residue of salivary peptide p-c. western blot analysis using extracts of human pancreata further showed that pancreatic p-c substance is not a precursor of insulin but a protein with molecular weight of 11,500 dalton, indicating the presence of a new protein in the insulin secretory granules of human pancreatic b-cells. |
1989-02-16 |
2023-08-11 |
human |
| J D Paulauskis, H S Su. Hormonal regulation of mouse fatty acid synthase gene transcription in liver. The Journal of biological chemistry. vol 264. issue 1. 1989-02-07. PMID:2535847. |
blot analysis of poly(a+) rna showed a rapid and dramatic insulin induction of fatty acid synthase (fas) mrna in diabetic mouse liver. |
1989-02-07 |
2023-08-11 |
mouse |
| P W Cook, W H Weintraub, K T Swanson, T E Machen, G L Fireston. Glucocorticoids confer normal serum/growth factor-dependent growth regulation to Fu5 rat hepatoma cells in vitro. Sequential expression of cell cycle-regulated genes without changes in intracellular calcium or pH. The Journal of biological chemistry. vol 263. issue 36. 1989-01-24. PMID:3058698. |
treatment of dexamethasone-suppressed quiescent fu5 with serum plus insulin caused a rapid reinitiation of cellular proliferation and dna synthesis that peaked at 16 h. rna dot blot analysis of this time course showed that the transcript levels for the proto-oncogenes c-fos, c-myc, and c-raski peaked at 0.5, 2, and 4 h, respectively, while expression of c-rasha and ornithine decarboxylase transcripts rose steadily during 16 h. microspectrofluorimetric measurements of cytosolic calcium (ca2+i) with fura-2 showed that insulin and serum, alone or in combination, elicited no changes in ca2+i over a 50-min time course, although atp, which is not a mitogen, induced large increases in ca2+i. |
1989-01-24 |
2023-08-11 |
rat |
| K M Kriauciunas, C R Kahn, D Muller-Wieland, S S Reddy, R Tau. Altered expression and function of the insulin receptor in a family with lipoatrophic diabetes. The Journal of clinical endocrinology and metabolism. vol 67. issue 6. 1989-01-03. PMID:2903867. |
to determine the role of genetic defects in the insulin receptor in the insulin resistance of lipoatrophic diabetes mellitus, we studied insulin binding, insulin receptor autophosphorylation, and insulin receptor mrna levels and performed southern blot analysis of genomic dna in four siblings, all of whom have some degree of insulin resistance and three of whom have lipoatrophy. |
1989-01-03 |
2023-08-11 |
Not clear |
| S E Ziska, M Bhattacharjee, R L Herber, P K Qasba, B K Vonderhaa. Thyroid hormone regulation of alpha-lactalbumin: differential glycosylation and messenger ribonucleic acid synthesis in mouse mammary glands. Endocrinology. vol 123. issue 5. 1988-11-21. PMID:3049049. |
northern blot analyses indicated that mammary gland tissue cultured in the presence of t3 contained 2.46 times more alpha-lactalbumin mrna than tissue cultured only in the presence of insulin, hydrocortisone, and prl. |
1988-11-21 |
2023-08-11 |
mouse |
| B Formby, A Ullrich, L Coussens, L Walker, C M Peterso. Growth hormone stimulates insulin gene expression in cultured human fetal pancreatic islets. The Journal of clinical endocrinology and metabolism. vol 66. issue 5. 1988-06-03. PMID:3283160. |
insulin gene expression was quantified by measuring insulin mrna by blot hybridization analysis using a [32p] cdna probe encoding human insulin. |
1988-06-03 |
2023-08-11 |
human |
| M Crettaz, D Muller-Wieland, C R Kah. Transcriptional and posttranscriptional regulation of tyrosine aminotransferase by insulin in rat hepatoma cells. Biochemistry. vol 27. issue 1. 1988-04-28. PMID:2894848. |
incubation of fao cells with insulin resulted in a 2-fold increase in tat activity and tat mrna measured by northern blot analysis with an oligonucleotide probe to the 5' end of the gene. |
1988-04-28 |
2023-08-11 |
rat |
| J L Messina, J Hamlin, J Larne. Positive interaction between insulin and phorbol esters on the regulation of a specific messenger ribonucleic acid in rat hepatoma cells. Endocrinology. vol 121. issue 4. 1987-11-04. PMID:3308433. |
with northern blot analysis, we demonstrate that phorbol 12-myristate 13-acetate (pma) stimulated p-33 messenger rna (mrna)-accumulation in rat hepatoma cells in a time- and concentration-dependent manner similar to insulin and plant lectins. |
1987-11-04 |
2023-08-11 |
rat |
| D S Straus, C D Takemot. Insulin negatively regulates albumin mRNA at the transcriptional and post-transcriptional level in rat hepatoma cells. The Journal of biological chemistry. vol 262. issue 5. 1987-03-30. PMID:2434475. |
northern blot analysis of purified poly(a)+ rna has indicated that insulin causes a decrease in albumin mrna relative to total cytoplasmic poly(a)+ rna. |
1987-03-30 |
2023-08-11 |
rat |
| M M Le Beau, T W McKeithan, E A Shima, R E Goldman-Leikin, S J Chan, G I Bell, J D Rowley, M O Dia. T-cell receptor alpha-chain gene is split in a human T-cell leukemia cell line with a t(11;14)(p15;q11). Proceedings of the National Academy of Sciences of the United States of America. vol 83. issue 24. 1987-02-06. PMID:3540949. |
by using in situ chromosomal hybridization and southern blot analysis to examine rpmi 8402 cells, we determined that the break at 14q11 occurs within the variable region sequences of the t-cell receptor alpha-chain gene (tcra); the break at 11p15 occurs between the hras1 gene and the genes for insulin and the insulin-like growth factor 2. |
1987-02-06 |
2023-08-11 |
human |
| P Rotwein, S L Naylor, J M Chirgwi. Human insulin-related DNA sequences map to chromosomes 2 and 11. Somatic cell and molecular genetics. vol 12. issue 6. 1987-01-14. PMID:3024335. |
two of these single copy dna sequences, hir1 and hir2, have been localized to chromosome 2 and 11p11----q13, respectively, by southern blot analysis of mouse-human somatic cell hybrids, a result consistent with other evidence supporting the dispersal of the insulin gene family throughout the genome. |
1987-01-14 |
2023-08-11 |
human |
| N S Ma, D S Gerhard, D E Housman, S Orkin, G Brun. Owl monkey gene mapping: the assignment of gene loci for catalase, beta-globin gene cluster, HRAS1, insulin, and parathyroid hormone. Cytogenetics and cell genetics. vol 43. issue 1-2. 1987-01-08. PMID:3536334. |
using somatic cell genetics and southern blot hybridization, we have mapped five structural genes in the owl monkey, coding for catalase (cat), the beta-globin gene cluster (hbbc), c-ha-ras 1 (hras1), insulin (ins), and parathyroid hormone (pth). |
1987-01-08 |
2023-08-11 |
mouse |
| U K Schubar. Regulation of gene expression in rat hepatocytes and hepatoma cells by insulin: quantitation of messenger ribonucleic acid's coding for tyrosine aminotransferase, tryptophan oxygenase, and phosphoenolpyruvate carboxykinase. Endocrinology. vol 119. issue 4. 1986-10-30. PMID:2875868. |
the effect of insulin on the abundance of mrnas coding for tyrosine aminotransferase (tat; ec 2.6.1.5), tryptophan oxygenase (to; ec 1.13.1.12), and p-enolpyruvate carboxykinase(gtp) (pepck; ec 4.1.1.32) was examined in primary cultures of adult rat hepatocytes and in fto-2b rat hepatoma cells by northern blot analysis using rna probes made from sp6-cdnas. |
1986-10-30 |
2023-08-11 |
rat |
| N Aoyama, T Nakamura, K Doi, S Baba, R Takahashi, T Sugiyam. Low frequency of 5'-flanking insertion of human insulin gene in Japanese non-insulin-dependent diabetic subjects. Diabetes care. vol 9. issue 4. 1986-10-23. PMID:3017653. |
polymorphism in the 5'-flanking region of the human insulin gene in 149 unrelated japanese subjects [77 with non-insulin-dependent diabetes mellitus (niddm), 17 with insulin-dependent diabetes mellitus (iddm), and 55 controls] was analyzed with southern blot hybridization. |
1986-10-23 |
2023-08-11 |
human |
| M A Permutt, T Andreone, J Chirgwin, S Elbein, P Rotwein, M Orlan. The genetics of type I and type II diabetes: analysis by recombinant DNA methodology. Advances in experimental medicine and biology. vol 189. 1985-10-24. PMID:3898768. |
the human insulin gene has been cloned and by southern blot hybridization analysis of peripheral leukocyte dna, the insulin gene locus is being evaluated as a possible contributor to the genetic defect. |
1985-10-24 |
2023-08-11 |
human |
| G A Hitman, J Katz, N Lytras, N I Jowett, J A Wass, G M Besser, D J Galto. Are there genetic determinants for the glucose intolerance of acromegaly? Clinical endocrinology. vol 22. issue 6. 1985-09-25. PMID:2990766. |
a genetic marker identifying the two parental insulin genes has been studied in 51 caucasian acromegalics by southern blot hybridization techniques using a cloned insulin gene probe. |
1985-09-25 |
2023-08-11 |
Not clear |
| E E Michalopoulos, P J Bevilacqua, N Stokoe, V E Powers, H F Willard, W H Lewi. Molecular analysis of gene deletion in aniridia--Wilms tumor association. Human genetics. vol 70. issue 2. 1985-08-01. PMID:2989154. |
the dna from the parental cells and the hybrid clones was screened by southern blot and dna hybridization with probes for the human insulin and ha-ras-1 genes. |
1985-08-01 |
2023-08-11 |
human |