| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Shi-Yan Li, Sara A B Gilbert, Qun Li, Jun Re. Aldehyde dehydrogenase-2 (ALDH2) ameliorates chronic alcohol ingestion-induced myocardial insulin resistance and endoplasmic reticulum stress. Journal of molecular and cellular cardiology. vol 47. issue 2. 2009-09-02. PMID:19344727. |
western blot analysis was used to assess insulin signaling at the levels of receptor, irs, akt, gsk-3beta, the transcription factors foxo3a, c-jun amino-terminal kinase (jnk) and c-jun. |
2009-09-02 |
2023-08-12 |
mouse |
| Jenny Vikman, Hjalmar Svensson, Ya-Chi Huang, Youhou Kang, Sofia A Andersson, Herbert Y Gaisano, Lena Eliasso. Truncation of SNAP-25 reduces the stimulatory action of cAMP on rapid exocytosis in insulin-secreting cells. American journal of physiology. Endocrinology and metabolism. vol 297. issue 2. 2009-08-18. PMID:19509185. |
to investigate the coupling between snap-25- and camp-dependent stimulation of insulin exocytosis, we have used capacitance measurements, protein-binding assays, and western blot analysis. |
2009-08-18 |
2023-08-12 |
Not clear |
| Luiz F Rezende, André S Vieira, Alessandro Negro, Francesco Langone, Antonio C Boscher. Ciliary neurotrophic factor (CNTF) signals through STAT3-SOCS3 pathway and protects rat pancreatic islets from cytokine-induced apoptosis. Cytokine. vol 46. issue 1. 2009-08-13. PMID:19272793. |
mrna expression (rt-pcr) and protein phosphorylation (western blot) of akt, erk1/2 and stat3, and socs-3 (rt-pcr and western blot), as well as glucose-stimulated insulin secretion (gsis) (radioimmunoassay), were analyzed. |
2009-08-13 |
2023-08-12 |
rat |
| B Maiztegui, M I Borelli, M A Raschia, H Del Zotto, J J Gagliardin. Islet adaptive changes to fructose-induced insulin resistance: beta-cell mass, glucokinase, glucose metabolism, and insulin secretion. The Journal of endocrinology. vol 200. issue 2. 2009-08-06. PMID:19039094. |
glucose-induced insulin release, glucose metabolism ((14)co(2) and (3)h(2)o production from d-[u-(14)c]- and d-[5-(3)h]-glucose) and hk/gk activity (g-6-p production), transcription (rt-pcr), protein expression (western blot), and cellular compartmentalization were measured in isolated islets (collagenase digestion). |
2009-08-06 |
2023-08-12 |
rat |
| O Gómez, B Ballester, A Romero, E Arnal, I Almansa, M Miranda, J E Mesonero, J Terrad. Expression and regulation of insulin and the glucose transporter GLUT8 in the testes of diabetic rats. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. vol 41. issue 5. 2009-08-03. PMID:19194835. |
testicular insulin levels measured by western blot were reduced by more than half in diabetic rats, although the distribution of the hormone was unchanged. |
2009-08-03 |
2023-08-12 |
rat |
| Everardo M Carneiro, Marcia Q Latorraca, Eliana Araujo, Marta Beltrá, Maria J Oliveras, Mónica Navarro, Genoveva Berná, Francisco J Bedoya, Licio A Velloso, Bernat Soria, Franz Martí. Taurine supplementation modulates glucose homeostasis and islet function. The Journal of nutritional biochemistry. vol 20. issue 7. 2009-07-23. PMID:18708284. |
insulin signaling was studied by western blot. |
2009-07-23 |
2023-08-12 |
mouse |
| Xian-qing Mao, Feng Yu, Nian Wang, Yong Wu, Feng Zou, Ke Wu, Min Liu, Jing-ping Ouyan. Hypoglycemic effect of polysaccharide enriched extract of Astragalus membranaceus in diet induced insulin resistant C57BL/6J mice and its potential mechanism. Phytomedicine : international journal of phytotherapy and phytopharmacology. vol 16. issue 5. 2009-07-02. PMID:19201177. |
the expression and activity of protein tyrosine phosphatase 1b (ptp1b), which plays a very important role in insulin signaling and in the er stress response, was measured by immunoprecipitation and western blot. |
2009-07-02 |
2023-08-12 |
mouse |
| Yu-Hua Zhang, He-Feng Wang, Wei Liu, Bin Wei, Lu-Jun Bing, Ying-Mao Ga. Insulin-producing cells derived from rat bone marrow and their autologous transplantation in the duodenal wall for treating diabetes. Anatomical record (Hoboken, N.J. : 2007). vol 292. issue 5. 2009-06-26. PMID:19382247. |
insulin production by the ipcs was confirmed by immunocytochemistry and western blot analysis. |
2009-06-26 |
2023-08-12 |
rat |
| H T Chiang, W H Cheng, P J Lu, H N Huang, W C Lo, Y C Tseng, J L Wang, M Hsiao, C J Tsen. Neuronal nitric oxide synthase activation is involved in insulin-mediated cardiovascular effects in the nucleus tractus solitarii of rats. Neuroscience. vol 159. issue 2. 2009-06-17. PMID:19167463. |
western blot and immunohistochemical analyses were performed to determine nnos phosphorylation levels after insulin or phosphoinositide 3-kinase (pi3k) inhibitor ly294002 microinjection into the nts. |
2009-06-17 |
2023-08-12 |
rat |
| H T Chiang, W H Cheng, P J Lu, H N Huang, W C Lo, Y C Tseng, J L Wang, M Hsiao, C J Tsen. Neuronal nitric oxide synthase activation is involved in insulin-mediated cardiovascular effects in the nucleus tractus solitarii of rats. Neuroscience. vol 159. issue 2. 2009-06-17. PMID:19167463. |
moreover, western blot analysis showed a significant increase in nnos (16.5+/-0.4-fold; p<0.05; n=4) phosphorylation after insulin injection, whereas the pi3k inhibitor ly294002 abolished the insulin-induced effects. |
2009-06-17 |
2023-08-12 |
rat |
| Haiyan Liu, Huiwen Yang, Dianlei Wang, Yuchun Liu, Xiaodong Liu, Yang Li, Lin Xie, GuangJi Wan. Insulin regulates P-glycoprotein in rat brain microvessel endothelial cells via an insulin receptor-mediated PKC/NF-kappaB pathway but not a PI3K/Akt pathway. European journal of pharmacology. vol 602. issue 2-3. 2009-03-30. PMID:19049803. |
the rat brain microvessel endothelial cells were incubated in normal culture medium containing 50 mu/l insulin and different concentrations of inhibitors for 72 h. the p-glycoprotein function and expression in the rat brain microvessel endothelial cells were assessed using the uptake of p-glycoprotein substrate rhodamine 123 and western blot assay, respectively. |
2009-03-30 |
2023-08-12 |
rat |
| Danilo G Ceschin, María C Sánchez, Gustavo A Chiabrand. Insulin induces the low density lipoprotein receptor-related protein 1 (LRP1) degradation by the proteasomal system in J774 macrophage-derived cells. Journal of cellular biochemistry. vol 106. issue 3. 2009-03-23. PMID:19115269. |
considering that macrophages play a central role in the pathogenesis of atherosclerosis, herein we evaluate the effect of insulin on the cellular expression of lrp1 in j774 macrophages-derived cells using western blot and immunofluorescence microscopy. |
2009-03-23 |
2023-08-12 |
Not clear |
| Jeremy A Lavine, Philipp W Raess, Dawn Belt Davis, Mary E Rabaglia, Brent K Presley, Mark P Keller, Margery C Beinfeld, Alan S Kopin, Christopher B Newgard, Alan D Atti. Overexpression of pre-pro-cholecystokinin stimulates beta-cell proliferation in mouse and human islets with retention of islet function. Molecular endocrinology (Baltimore, Md.). vol 22. issue 12. 2009-03-06. PMID:18845673. |
we studied islet function by measuring glucose-stimulated insulin secretion and investigated the cell cycle regulation of proliferating beta-cells by quantitative rt-pcr and western blot analysis. |
2009-03-06 |
2023-08-12 |
mouse |
| Maryam Moosavi, Nader Maghsoudi, Saleh Zahedi-Asl, Nasser Naghdi, Mitra Yousefpour, Ian Andrew Trounc. The role of PI3/Akt pathway in the protective effect of insulin against corticosterone cell death induction in hippocampal cell culture. Neuroendocrinology. vol 88. issue 4. 2009-02-19. PMID:18679014. |
western blot analyses showed that insulin induced the activation of protein kinase b (akt). |
2009-02-19 |
2023-08-12 |
Not clear |
| Q X Yuan, L P Teng, J Y Zhou, C P Liu, J Guo, L J Liu, W De, C Li. Characterization of Munc13-1 and insulin secretion during pancreatic development in rats. Journal of endocrinological investigation. vol 31. issue 7. 2008-12-22. PMID:18787382. |
the expression of munc13-1 and insulin secretion was studied by the techniques of rtpcr, real-time pcr, western blot, and enzyme-linked immunosorbent assay. |
2008-12-22 |
2023-08-12 |
rat |
| BingZhi Yu, Zhe Zhang, Xin Deng, XiaoYan Xu, Chen Feng, YanXiao Li, Cheng Cui, WenHui Su, HongMei Zhao, DaHai Y. Involvement of insulin in early development of mouse one-cell stage embryos. Science in China. Series C, Life sciences. vol 51. issue 9. 2008-11-04. PMID:18726521. |
in the present study, we examined the expression and localization of insulin in the mouse oocytes and one-cell stage embryos by quantitative elisa, rt-pcr, western blot and immunofluorescence. |
2008-11-04 |
2023-08-12 |
mouse |
| Michael Hsiao, Pei-Jung Lu, Hsiao-Ning Huang, Wan-Chen Lo, Wen-Yu Ho, Tsung-Ching Lai, Hung-Ting Chiang, Ching-Jiunn Tsen. Defective phosphatidylinositol 3-kinase signaling in central control of cardiovascular effects in the nucleus tractus solitarii of spontaneously hypertensive rats. Hypertension research : official journal of the Japanese Society of Hypertension. vol 31. issue 6. 2008-09-09. PMID:18716370. |
western blot analysis showed no significant increase in akt phosphorylation in 8-week-old pre-hypertensive shr after insulin injection. |
2008-09-09 |
2023-08-12 |
rat |
| Christian Freund, Dorien Ward-van Oostwaard, Jantine Monshouwer-Kloots, Stieneke van den Brink, Marga van Rooijen, Xiuqin Xu, Robert Zweigerdt, Christine Mummery, Robert Passie. Insulin redirects differentiation from cardiogenic mesoderm and endoderm to neuroectoderm in differentiating human embryonic stem cells. Stem cells (Dayton, Ohio). vol 26. issue 3. 2008-05-07. PMID:18096723. |
real-time reverse transcription-polymerase chain reaction and western blot analysis showed that insulin inhibited cardiomyogenesis in the early phase of coculture by suppressing the expression of endoderm (foxa2, gata-6), mesoderm (brachyury t), and cardiac mesoderm (nkx2.5, gata-4). |
2008-05-07 |
2023-08-12 |
human |
| Haiyan Liu, Xiaodong Liu, Lee Jia, Yuchun Liu, Huiwen Yang, Guangji Wang, Lin Xi. Insulin therapy restores impaired function and expression of P-glycoprotein in blood-brain barrier of experimental diabetes. Biochemical pharmacology. vol 75. issue 8. 2008-04-22. PMID:18299117. |
in vitro effects of insulin on function and expression of p-gp in primarily cultured rat brain microvessel endothelial cells (rbmecs) were evaluated using rhodamine 123 (rho123) uptakes and western blot, respectively. |
2008-04-22 |
2023-08-12 |
rat |
| N Montserrat, J Sánchez-Gurmaches, D García de la Serrana, M I Navarro, J Gutiérre. IGF-I binding and receptor signal transduction in primary cell culture of muscle cells of gilthead sea bream: changes throughout in vitro development. Cell and tissue research. vol 330. issue 3. 2008-04-11. PMID:17940802. |
the activation of various kinase (erk 1/2 mapk and akt/pkb) proteins by igfs and insulin was studied by means of western blot analysis. |
2008-04-11 |
2023-08-12 |
Not clear |