| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wei Chen, Yi-Ming Li, Mao-Hua Y. Astragalus polysaccharides inhibited diabetic cardiomyopathy in hamsters depending on suppression of heart chymase activation. Journal of diabetes and its complications. vol 24. issue 3. 2010-07-16. PMID:19230716. |
after aps/ns administration at a dose of 1 g/kg per day for 10 weeks, hemodynamic parameters, levels of insulin (ins), c-peptide (c-p), glycosylated serum protein (gsp), lipoproteins, myocardial enzymes, and ang ii (plasma and myocardial) were tested; myocardial collagen (type i and iii), myocardial ultrastructure, and activities of matrix metalloproteinase (mmps) were measured; activities and expression of cardiac chymase and ace were detected by using quantitative real-time rt-pcr and ria; protein expression of cardiac phosphoric extracellular signal-regulated kinase 1/2 (p-erk1/2) was measured by western blot. |
2010-07-16 |
2023-08-12 |
Not clear |
| Josiane Faganello, Valéria Dutra, Augusto Schrank, Wieland Meyer, Irene S Schrank, Marilene H Vainstei. Identification of genomic differences between Cryptococcus neoformans and Cryptococcus gattii by Representational Difference Analysis (RDA). Medical mycology. vol 47. issue 6. 2010-03-01. PMID:18668420. |
southern blot experiments using five selected clones confirmed the presence of polymorphisms for three sequences (a putative insulin degrading enzyme (ide), a chitin synthase and an endoplasmic reticulum protein). |
2010-03-01 |
2023-08-12 |
Not clear |
| Christopher M Mayer, Denise D Belsha. Palmitate attenuates insulin signaling and induces endoplasmic reticulum stress and apoptosis in hypothalamic neurons: rescue of resistance and apoptosis through adenosine 5' monophosphate-activated protein kinase activation. Endocrinology. vol 151. issue 2. 2010-02-26. PMID:19952270. |
through western blot analysis, we discerned that prolonged exposure to palmitate impairs insulin activation, as assessed by phosphorylation of akt. |
2010-02-26 |
2023-08-12 |
Not clear |
| Christopher M Mayer, Denise D Belsha. Central insulin signaling is attenuated by long-term insulin exposure via insulin receptor substrate-1 serine phosphorylation, proteasomal degradation, and lysosomal insulin receptor degradation. Endocrinology. vol 151. issue 1. 2010-02-22. PMID:19887566. |
western blot analysis indicated that prolonged insulin treatment of mhypoe-46 cells attenuated insulin signaling through phospho-akt. |
2010-02-22 |
2023-08-12 |
human |
| Megan E Bardgett, John J McCarthy, Sean D Stocke. Glutamatergic receptor activation in the rostral ventrolateral medulla mediates the sympathoexcitatory response to hyperinsulinemia. Hypertension (Dallas, Tex. : 1979). vol 55. issue 2. 2010-02-22. PMID:20065145. |
western blot analysis showed that insulin receptor expression is significantly lower in the rvlm than the hypothalamus, and direct microinjection of insulin into the rvlm did not significantly increase lumbar sna. |
2010-02-22 |
2023-08-12 |
rat |
| Hu Zhou, Yuanyuan Xiao, Rongxia Li, Shangyu Hong, Sujun Li, Lianshui Wang, Rong Zeng, Kan Lia. Quantitative analysis of secretome from adipocytes regulated by insulin. Acta biochimica et biophysica Sinica. vol 41. issue 11. 2010-01-13. PMID:19902125. |
western blot analysis of these adipokines confirmed the quantitative results from mass spectrometry, and revealed individualized secreting patterns of these proteins by increasing insulin dose. |
2010-01-13 |
2023-08-12 |
Not clear |
| Shi-Yan Li, Sara A B Gilbert, Qun Li, Jun Re. Aldehyde dehydrogenase-2 (ALDH2) ameliorates chronic alcohol ingestion-induced myocardial insulin resistance and endoplasmic reticulum stress. Journal of molecular and cellular cardiology. vol 47. issue 2. 2009-09-02. PMID:19344727. |
western blot analysis was used to assess insulin signaling at the levels of receptor, irs, akt, gsk-3beta, the transcription factors foxo3a, c-jun amino-terminal kinase (jnk) and c-jun. |
2009-09-02 |
2023-08-12 |
mouse |
| Jenny Vikman, Hjalmar Svensson, Ya-Chi Huang, Youhou Kang, Sofia A Andersson, Herbert Y Gaisano, Lena Eliasso. Truncation of SNAP-25 reduces the stimulatory action of cAMP on rapid exocytosis in insulin-secreting cells. American journal of physiology. Endocrinology and metabolism. vol 297. issue 2. 2009-08-18. PMID:19509185. |
to investigate the coupling between snap-25- and camp-dependent stimulation of insulin exocytosis, we have used capacitance measurements, protein-binding assays, and western blot analysis. |
2009-08-18 |
2023-08-12 |
Not clear |
| Luiz F Rezende, André S Vieira, Alessandro Negro, Francesco Langone, Antonio C Boscher. Ciliary neurotrophic factor (CNTF) signals through STAT3-SOCS3 pathway and protects rat pancreatic islets from cytokine-induced apoptosis. Cytokine. vol 46. issue 1. 2009-08-13. PMID:19272793. |
mrna expression (rt-pcr) and protein phosphorylation (western blot) of akt, erk1/2 and stat3, and socs-3 (rt-pcr and western blot), as well as glucose-stimulated insulin secretion (gsis) (radioimmunoassay), were analyzed. |
2009-08-13 |
2023-08-12 |
rat |
| B Maiztegui, M I Borelli, M A Raschia, H Del Zotto, J J Gagliardin. Islet adaptive changes to fructose-induced insulin resistance: beta-cell mass, glucokinase, glucose metabolism, and insulin secretion. The Journal of endocrinology. vol 200. issue 2. 2009-08-06. PMID:19039094. |
glucose-induced insulin release, glucose metabolism ((14)co(2) and (3)h(2)o production from d-[u-(14)c]- and d-[5-(3)h]-glucose) and hk/gk activity (g-6-p production), transcription (rt-pcr), protein expression (western blot), and cellular compartmentalization were measured in isolated islets (collagenase digestion). |
2009-08-06 |
2023-08-12 |
rat |
| O Gómez, B Ballester, A Romero, E Arnal, I Almansa, M Miranda, J E Mesonero, J Terrad. Expression and regulation of insulin and the glucose transporter GLUT8 in the testes of diabetic rats. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. vol 41. issue 5. 2009-08-03. PMID:19194835. |
testicular insulin levels measured by western blot were reduced by more than half in diabetic rats, although the distribution of the hormone was unchanged. |
2009-08-03 |
2023-08-12 |
rat |
| Everardo M Carneiro, Marcia Q Latorraca, Eliana Araujo, Marta Beltrá, Maria J Oliveras, Mónica Navarro, Genoveva Berná, Francisco J Bedoya, Licio A Velloso, Bernat Soria, Franz Martí. Taurine supplementation modulates glucose homeostasis and islet function. The Journal of nutritional biochemistry. vol 20. issue 7. 2009-07-23. PMID:18708284. |
insulin signaling was studied by western blot. |
2009-07-23 |
2023-08-12 |
mouse |
| Xian-qing Mao, Feng Yu, Nian Wang, Yong Wu, Feng Zou, Ke Wu, Min Liu, Jing-ping Ouyan. Hypoglycemic effect of polysaccharide enriched extract of Astragalus membranaceus in diet induced insulin resistant C57BL/6J mice and its potential mechanism. Phytomedicine : international journal of phytotherapy and phytopharmacology. vol 16. issue 5. 2009-07-02. PMID:19201177. |
the expression and activity of protein tyrosine phosphatase 1b (ptp1b), which plays a very important role in insulin signaling and in the er stress response, was measured by immunoprecipitation and western blot. |
2009-07-02 |
2023-08-12 |
mouse |
| Yu-Hua Zhang, He-Feng Wang, Wei Liu, Bin Wei, Lu-Jun Bing, Ying-Mao Ga. Insulin-producing cells derived from rat bone marrow and their autologous transplantation in the duodenal wall for treating diabetes. Anatomical record (Hoboken, N.J. : 2007). vol 292. issue 5. 2009-06-26. PMID:19382247. |
insulin production by the ipcs was confirmed by immunocytochemistry and western blot analysis. |
2009-06-26 |
2023-08-12 |
rat |
| H T Chiang, W H Cheng, P J Lu, H N Huang, W C Lo, Y C Tseng, J L Wang, M Hsiao, C J Tsen. Neuronal nitric oxide synthase activation is involved in insulin-mediated cardiovascular effects in the nucleus tractus solitarii of rats. Neuroscience. vol 159. issue 2. 2009-06-17. PMID:19167463. |
western blot and immunohistochemical analyses were performed to determine nnos phosphorylation levels after insulin or phosphoinositide 3-kinase (pi3k) inhibitor ly294002 microinjection into the nts. |
2009-06-17 |
2023-08-12 |
rat |
| H T Chiang, W H Cheng, P J Lu, H N Huang, W C Lo, Y C Tseng, J L Wang, M Hsiao, C J Tsen. Neuronal nitric oxide synthase activation is involved in insulin-mediated cardiovascular effects in the nucleus tractus solitarii of rats. Neuroscience. vol 159. issue 2. 2009-06-17. PMID:19167463. |
moreover, western blot analysis showed a significant increase in nnos (16.5+/-0.4-fold; p<0.05; n=4) phosphorylation after insulin injection, whereas the pi3k inhibitor ly294002 abolished the insulin-induced effects. |
2009-06-17 |
2023-08-12 |
rat |
| Haiyan Liu, Huiwen Yang, Dianlei Wang, Yuchun Liu, Xiaodong Liu, Yang Li, Lin Xie, GuangJi Wan. Insulin regulates P-glycoprotein in rat brain microvessel endothelial cells via an insulin receptor-mediated PKC/NF-kappaB pathway but not a PI3K/Akt pathway. European journal of pharmacology. vol 602. issue 2-3. 2009-03-30. PMID:19049803. |
the rat brain microvessel endothelial cells were incubated in normal culture medium containing 50 mu/l insulin and different concentrations of inhibitors for 72 h. the p-glycoprotein function and expression in the rat brain microvessel endothelial cells were assessed using the uptake of p-glycoprotein substrate rhodamine 123 and western blot assay, respectively. |
2009-03-30 |
2023-08-12 |
rat |
| Danilo G Ceschin, María C Sánchez, Gustavo A Chiabrand. Insulin induces the low density lipoprotein receptor-related protein 1 (LRP1) degradation by the proteasomal system in J774 macrophage-derived cells. Journal of cellular biochemistry. vol 106. issue 3. 2009-03-23. PMID:19115269. |
considering that macrophages play a central role in the pathogenesis of atherosclerosis, herein we evaluate the effect of insulin on the cellular expression of lrp1 in j774 macrophages-derived cells using western blot and immunofluorescence microscopy. |
2009-03-23 |
2023-08-12 |
Not clear |
| Jeremy A Lavine, Philipp W Raess, Dawn Belt Davis, Mary E Rabaglia, Brent K Presley, Mark P Keller, Margery C Beinfeld, Alan S Kopin, Christopher B Newgard, Alan D Atti. Overexpression of pre-pro-cholecystokinin stimulates beta-cell proliferation in mouse and human islets with retention of islet function. Molecular endocrinology (Baltimore, Md.). vol 22. issue 12. 2009-03-06. PMID:18845673. |
we studied islet function by measuring glucose-stimulated insulin secretion and investigated the cell cycle regulation of proliferating beta-cells by quantitative rt-pcr and western blot analysis. |
2009-03-06 |
2023-08-12 |
mouse |
| Maryam Moosavi, Nader Maghsoudi, Saleh Zahedi-Asl, Nasser Naghdi, Mitra Yousefpour, Ian Andrew Trounc. The role of PI3/Akt pathway in the protective effect of insulin against corticosterone cell death induction in hippocampal cell culture. Neuroendocrinology. vol 88. issue 4. 2009-02-19. PMID:18679014. |
western blot analyses showed that insulin induced the activation of protein kinase b (akt). |
2009-02-19 |
2023-08-12 |
Not clear |