| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Luiz F Rezende, Rafael L Camargo, Renato C S Branco, Ana P G Cappelli, Antonio C Boschero, Everardo M Carneir. Reduced insulin clearance and lower insulin-degrading enzyme expression in the liver might contribute to the thrifty phenotype of protein-restricted mice. The British journal of nutrition. vol 112. issue 6. 2014-11-24. PMID:25036874. |
insulin concentrations were determined using ria and protein expression and phosphorylation by western blot analysis. |
2014-11-24 |
2023-08-13 |
mouse |
| Jingbo Liu, Jian He, Yuekui Yang, Jie Yu, Xiangbing Mao, Bing Yu, Daiwen Che. Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs. Archives of animal nutrition. vol 68. issue 2. 2014-11-17. PMID:24646150. |
western blot analysis indicated that hf feeding elevated the protein expressions of tlr4 signalling pathway, as tlr4, myd88, iκb kinase β and nuclear factor-κb, and insulin signalling-related proteins, as phosphorylated insulin receptor substrate-1 and phosphorylated protein kinase b. |
2014-11-17 |
2023-08-12 |
Not clear |
| Michelle Bamji-Mirza, Debbie Callaghan, Dema Najem, Shanshan Shen, Mohamed Shaad Hasim, Ze Yang, Wandong Zhan. Stimulation of insulin signaling and inhibition of JNK-AP1 activation protect cells from amyloid-β-induced signaling dysregulation and inflammatory response. Journal of Alzheimer's disease : JAD. vol 40. issue 1. 2014-10-30. PMID:24346217. |
transcription factor profiling showed that treatment of ihbec with aβ1-42, insulin, or sp600125 alone or in combination resulted in profound changes in modulating the activities of multiple transcription factors and relevant pathways, some of which were validated by western blot. |
2014-10-30 |
2023-08-12 |
human |
| Y Hu, X-H Xu, K He, L-L Zhang, S-K Wang, Y-Q Pan, B-S He, T-T Feng, X-M Ma. Genome-wide analysis of DNA methylation variations caused by chronic glucolipotoxicity in beta-cells. Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association. vol 122. issue 2. 2014-10-15. PMID:24554506. |
the mrna and protein expression of insulin and tcf7l2 in these cells were quantified by rt-pcr and western blot, respectively.we have identified a lot of loci which experienced aberrant dna methylation in beta-cells cultured with high-glucose-lipid medium. |
2014-10-15 |
2023-08-12 |
Not clear |
| Yun-A Jung, Hee Kyoung Kim, Kwi-Hyun Bae, Hye-Young Seo, Hye-Soon Kim, Byoung Kuk Jang, Gwon-Soo Jung, In-Kyu Lee, Mi-Kyung Kim, Keun-Gyu Par. Cilostazol inhibits insulin-stimulated expression of sterol regulatory binding protein-1c via inhibition of LXR and Sp1. Experimental & molecular medicine. vol 46. 2014-09-25. PMID:24458133. |
the effects of cilostazol on the expression of srebp-1c and its target genes in response to insulin or an lxr agonist (t0901317) were examined using real-time rt-pcr and western blot analysis on cultured hepatocytes. |
2014-09-25 |
2023-08-12 |
Not clear |
| So Yeon Park, Min Hye Kim, Joung Hoon Ahn, Su Jin Lee, Jong Ho Lee, Won Sik Eum, Soo Young Choi, Hyeok Yil Kwo. The Stimulatory Effect of Essential Fatty Acids on Glucose Uptake Involves Both Akt and AMPK Activation in C2C12 Skeletal Muscle Cells. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology. vol 18. issue 3. 2014-06-30. PMID:24976766. |
two efas and oa significantly protected pa-induced suppression of insulin signaling, respectively, which was confirmed by the increased levels of akt phosphorylation and serine/threonine kinases (pkcθ and jnk) dephosphorylation in the western blot analysis. |
2014-06-30 |
2023-08-13 |
Not clear |
| Snežana Tepavčević, Danijela Vojnović Milutinović, Djuro Macut, Zorica Žakula, Marina Nikolić, Ivana Božić-Antić, Snježana Romić, Jelica Bjekić-Macut, Gordana Matić, Goran Korićana. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. The Journal of steroid biochemistry and molecular biology. vol 141. 2014-05-27. PMID:24472754. |
expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by western blot analysis in dht-treated and control rats. |
2014-05-27 |
2023-08-12 |
rat |
| Chin Piow Wong, Toshio Kaneda, A Hamid A Hadi, Hiroshi Morit. Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus. Journal of natural medicines. vol 68. issue 1. 2014-05-21. PMID:23494817. |
western blot analysis showed that ceramicine b was found to inhibit the phosphorylation of forkhead box o1 (foxo1), a key process in the insulin signaling pathway. |
2014-05-21 |
2023-08-12 |
mouse |
| Xiangxiang Shan, Yufeng Miao, Rengen Fan, Changzhi Song, Guangzhou Wu, Zhengqiang Wan, Jian Zhu, Guan Sun, Wenzhang Zha, Xiangming Mu, Guangjun Zhou, Yan Che. Suppression of Grb2 expression improved hepatic steatosis, oxidative stress, and apoptosis induced by palmitic acid in vitro partly through insulin signaling alteration. In vitro cellular & developmental biology. Animal. vol 49. issue 8. 2014-05-06. PMID:23771793. |
quantitative polymerase chain reaction and western blot were used to measure expressional changes of key markers of insulin signaling, lipid/glucose metabolism, oxidative stress, and apoptosis. |
2014-05-06 |
2023-08-12 |
Not clear |
| Charlott Mattes, Mandy Laube, Ulrich H Thom. Rapid elevation of sodium transport through insulin is mediated by AKT in alveolar cells. Physiological reports. vol 2. issue 3. 2014-04-24. PMID:24760523. |
in western blot measurements the activation of mediators stimulated by insulin was analyzed. |
2014-04-24 |
2023-08-13 |
rat |
| Charlott Mattes, Mandy Laube, Ulrich H Thom. Rapid elevation of sodium transport through insulin is mediated by AKT in alveolar cells. Physiological reports. vol 2. issue 3. 2014-04-24. PMID:24760523. |
western blot measurements revealed an increased phosphorylation of akt after insulin stimulation. |
2014-04-24 |
2023-08-13 |
rat |
| Charlott Mattes, Mandy Laube, Ulrich H Thom. Rapid elevation of sodium transport through insulin is mediated by AKT in alveolar cells. Physiological reports. vol 2. issue 3. 2014-04-24. PMID:24760523. |
sgk1 activity was also increased by insulin as shown by western blot of pndrg1. |
2014-04-24 |
2023-08-13 |
rat |
| Susanne Lager, Anne-Maj Samulesson, Paul D Taylor, Lucilla Poston, Theresa L Powell, Thomas Jansso. Diet-induced obesity in mice reduces placental efficiency and inhibits placental mTOR signaling. Physiological reports. vol 2. issue 2. 2014-04-18. PMID:24744907. |
using western blot analysis, placental mtor activity was determined along with energy, inflammatory, and insulin signaling pathways (upstream modulators of mtor). |
2014-04-18 |
2023-08-13 |
mouse |
| Antonio Pérez-Pérez, Julieta Maymó, Yesica Gambino, Pilar Guadix, José L Dueñas, Cecilia Varone, Víctor Sánchez-Margale. Insulin enhances leptin expression in human trophoblastic cells. Biology of reproduction. vol 89. issue 1. 2014-03-05. PMID:23718986. |
western blot analysis and quantitative real time rt-pcr was performed to determine the leptin expression level after treatment of cells or trophoblast explants with different concentrations of insulin (0.1-100 nm). |
2014-03-05 |
2023-08-12 |
human |
| Xin Wang, Wei Zhang, Hongli Chen, Nai Liao, Zhao Wang, Xiaodi Zhang, Chunxu Ha. High selenium impairs hepatic insulin sensitivity through opposite regulation of ROS. Toxicology letters. vol 224. issue 1. 2014-02-06. PMID:24140496. |
insulin sensitivity and glucose/lipid metabolism were determined by glucose/insulin tolerance test, western blot, immunofluorescence, specific probes and other biochemical assays. |
2014-02-06 |
2023-08-12 |
rat |
| Anaies Nazarians-Armavil, Jennifer A Chalmers, Claire B Lee, Wenqing Ye, Denise D Belsha. Cellular insulin resistance disrupts hypothalamic mHypoA-POMC/GFP neuronal signaling pathways. The Journal of endocrinology. vol 220. issue 1. 2014-01-30. PMID:24134870. |
furthermore, the activation of ir-mediated downstream second messengers was examined by western blot analysis, following the induction of cellular insulin resistance, which resulted in a loss of insulin-mediated regulation of pomc and ir mrnas. |
2014-01-30 |
2023-08-12 |
mouse |
| Jialin Gao, Xuefan Gu, Don J Mahuran, Zhugang Wang, Huiwen Zhan. Impaired glucose tolerance in a mouse model of sidt2 deficiency. PloS one. vol 8. issue 6. 2014-01-16. PMID:23776622. |
by western blot and immunofluorescence, sidt2(-/-) mouse islets were shown to have increased insulin protein, which actually contained more insulin secretory granules than their controls, demonstrated by electromicroscopy. |
2014-01-16 |
2023-08-12 |
mouse |
| J Bian, X M Bai, Y L Zhao, L Zhang, Z J Li. Lentiviral vector-mediated knockdown of Lrb in the arcuate nucleus promotes diet-induced obesity in rats. Journal of molecular endocrinology. vol 51. issue 1. 2014-01-07. PMID:23549406. |
after 8 weeks of the diet, serum leptin, and insulin concentrations were measured by ria, gene expression of lrb in the arc was detected by a real-time rt-pcr, and leptin signaling was examined by western blot. |
2014-01-07 |
2023-08-12 |
human |
| Adam Gesing, Andrzej Bartke, Michal M Masterna. Pioglitazone does not improve insulin signaling in mice with GH over-expression. The Journal of endocrinology. vol 219. issue 2. 2013-12-03. PMID:23946430. |
plasma levels of insulin and glucose as well as hepatic levels of proteins involved in insulin signaling were analyzed by elisa or western blot methods. |
2013-12-03 |
2023-08-12 |
mouse |
| P Brandimarti, J M Costa-Júnior, S M Ferreira, A O Protzek, G J Santos, E M Carneiro, A C Boschero, L F Rezend. Cafeteria diet inhibits insulin clearance by reduced insulin-degrading enzyme expression and mRNA splicing. The Journal of endocrinology. vol 219. issue 2. 2013-12-03. PMID:23959080. |
we then isolated pancreatic islets for ex vivo glucose-stimulated insulin secretion as well as liver, gastrocnemius, visceral adipose tissue, and hypothalamus for subsequent protein analysis by western blot and determination of mrna levels by real-time rt-pcr. |
2013-12-03 |
2023-08-12 |
mouse |