| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Snežana Tepavčević, Danijela Vojnović Milutinović, Djuro Macut, Zorica Žakula, Marina Nikolić, Ivana Božić-Antić, Snježana Romić, Jelica Bjekić-Macut, Gordana Matić, Goran Korićana. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. The Journal of steroid biochemistry and molecular biology. vol 141. 2014-05-27. PMID:24472754. |
expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by western blot analysis in dht-treated and control rats. |
2014-05-27 |
2023-08-12 |
rat |
| Chin Piow Wong, Toshio Kaneda, A Hamid A Hadi, Hiroshi Morit. Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus. Journal of natural medicines. vol 68. issue 1. 2014-05-21. PMID:23494817. |
western blot analysis showed that ceramicine b was found to inhibit the phosphorylation of forkhead box o1 (foxo1), a key process in the insulin signaling pathway. |
2014-05-21 |
2023-08-12 |
mouse |
| Xiangxiang Shan, Yufeng Miao, Rengen Fan, Changzhi Song, Guangzhou Wu, Zhengqiang Wan, Jian Zhu, Guan Sun, Wenzhang Zha, Xiangming Mu, Guangjun Zhou, Yan Che. Suppression of Grb2 expression improved hepatic steatosis, oxidative stress, and apoptosis induced by palmitic acid in vitro partly through insulin signaling alteration. In vitro cellular & developmental biology. Animal. vol 49. issue 8. 2014-05-06. PMID:23771793. |
quantitative polymerase chain reaction and western blot were used to measure expressional changes of key markers of insulin signaling, lipid/glucose metabolism, oxidative stress, and apoptosis. |
2014-05-06 |
2023-08-12 |
Not clear |
| Charlott Mattes, Mandy Laube, Ulrich H Thom. Rapid elevation of sodium transport through insulin is mediated by AKT in alveolar cells. Physiological reports. vol 2. issue 3. 2014-04-24. PMID:24760523. |
in western blot measurements the activation of mediators stimulated by insulin was analyzed. |
2014-04-24 |
2023-08-13 |
rat |
| Charlott Mattes, Mandy Laube, Ulrich H Thom. Rapid elevation of sodium transport through insulin is mediated by AKT in alveolar cells. Physiological reports. vol 2. issue 3. 2014-04-24. PMID:24760523. |
western blot measurements revealed an increased phosphorylation of akt after insulin stimulation. |
2014-04-24 |
2023-08-13 |
rat |
| Charlott Mattes, Mandy Laube, Ulrich H Thom. Rapid elevation of sodium transport through insulin is mediated by AKT in alveolar cells. Physiological reports. vol 2. issue 3. 2014-04-24. PMID:24760523. |
sgk1 activity was also increased by insulin as shown by western blot of pndrg1. |
2014-04-24 |
2023-08-13 |
rat |
| Susanne Lager, Anne-Maj Samulesson, Paul D Taylor, Lucilla Poston, Theresa L Powell, Thomas Jansso. Diet-induced obesity in mice reduces placental efficiency and inhibits placental mTOR signaling. Physiological reports. vol 2. issue 2. 2014-04-18. PMID:24744907. |
using western blot analysis, placental mtor activity was determined along with energy, inflammatory, and insulin signaling pathways (upstream modulators of mtor). |
2014-04-18 |
2023-08-13 |
mouse |
| Antonio Pérez-Pérez, Julieta Maymó, Yesica Gambino, Pilar Guadix, José L Dueñas, Cecilia Varone, Víctor Sánchez-Margale. Insulin enhances leptin expression in human trophoblastic cells. Biology of reproduction. vol 89. issue 1. 2014-03-05. PMID:23718986. |
western blot analysis and quantitative real time rt-pcr was performed to determine the leptin expression level after treatment of cells or trophoblast explants with different concentrations of insulin (0.1-100 nm). |
2014-03-05 |
2023-08-12 |
human |
| Xin Wang, Wei Zhang, Hongli Chen, Nai Liao, Zhao Wang, Xiaodi Zhang, Chunxu Ha. High selenium impairs hepatic insulin sensitivity through opposite regulation of ROS. Toxicology letters. vol 224. issue 1. 2014-02-06. PMID:24140496. |
insulin sensitivity and glucose/lipid metabolism were determined by glucose/insulin tolerance test, western blot, immunofluorescence, specific probes and other biochemical assays. |
2014-02-06 |
2023-08-12 |
rat |
| Anaies Nazarians-Armavil, Jennifer A Chalmers, Claire B Lee, Wenqing Ye, Denise D Belsha. Cellular insulin resistance disrupts hypothalamic mHypoA-POMC/GFP neuronal signaling pathways. The Journal of endocrinology. vol 220. issue 1. 2014-01-30. PMID:24134870. |
furthermore, the activation of ir-mediated downstream second messengers was examined by western blot analysis, following the induction of cellular insulin resistance, which resulted in a loss of insulin-mediated regulation of pomc and ir mrnas. |
2014-01-30 |
2023-08-12 |
mouse |
| Jialin Gao, Xuefan Gu, Don J Mahuran, Zhugang Wang, Huiwen Zhan. Impaired glucose tolerance in a mouse model of sidt2 deficiency. PloS one. vol 8. issue 6. 2014-01-16. PMID:23776622. |
by western blot and immunofluorescence, sidt2(-/-) mouse islets were shown to have increased insulin protein, which actually contained more insulin secretory granules than their controls, demonstrated by electromicroscopy. |
2014-01-16 |
2023-08-12 |
mouse |
| J Bian, X M Bai, Y L Zhao, L Zhang, Z J Li. Lentiviral vector-mediated knockdown of Lrb in the arcuate nucleus promotes diet-induced obesity in rats. Journal of molecular endocrinology. vol 51. issue 1. 2014-01-07. PMID:23549406. |
after 8 weeks of the diet, serum leptin, and insulin concentrations were measured by ria, gene expression of lrb in the arc was detected by a real-time rt-pcr, and leptin signaling was examined by western blot. |
2014-01-07 |
2023-08-12 |
human |
| Adam Gesing, Andrzej Bartke, Michal M Masterna. Pioglitazone does not improve insulin signaling in mice with GH over-expression. The Journal of endocrinology. vol 219. issue 2. 2013-12-03. PMID:23946430. |
plasma levels of insulin and glucose as well as hepatic levels of proteins involved in insulin signaling were analyzed by elisa or western blot methods. |
2013-12-03 |
2023-08-12 |
mouse |
| P Brandimarti, J M Costa-Júnior, S M Ferreira, A O Protzek, G J Santos, E M Carneiro, A C Boschero, L F Rezend. Cafeteria diet inhibits insulin clearance by reduced insulin-degrading enzyme expression and mRNA splicing. The Journal of endocrinology. vol 219. issue 2. 2013-12-03. PMID:23959080. |
we then isolated pancreatic islets for ex vivo glucose-stimulated insulin secretion as well as liver, gastrocnemius, visceral adipose tissue, and hypothalamus for subsequent protein analysis by western blot and determination of mrna levels by real-time rt-pcr. |
2013-12-03 |
2023-08-12 |
mouse |
| Manabu Ishiki, Yasuhiro Nishida, Hiroshi Ishibashi, Tsutomu Wada, Shiho Fujisaka, Akiko Takikawa, Masaharu Urakaze, Toshiyasu Sasaoka, Isao Usui, Kazuyuki Tob. Impact of divergent effects of astaxanthin on insulin signaling in L6 cells. Endocrinology. vol 154. issue 8. 2013-10-29. PMID:23715867. |
we examined the effect of astaxanthin on insulin-stimulated glucose transporter 4 (glut4) translocation, glucose uptake, and insulin signaling in cultured rat l6 muscle cells using plasma membrane lawn assay, 2-deoxyglucose uptake, and western blot analysis. |
2013-10-29 |
2023-08-12 |
rat |
| Rongjing Song, Xi Wang, Yiqing Mao, Hui Li, Zhixin Li, Wei Xu, Rong Wang, Tingting Guo, Ling Jin, Xiaojing Zhang, Yizhuang Zhang, Na Zhou, Ruobi Hu, Jianwei Jia, Zhen Lei, David M Irwin, Gang Niu, Huanran Ta. Resistin disrupts glycogen synthesis under high insulin and high glucose levels by down-regulating the hepatic levels of GSK3β. Gene. vol 529. issue 1. 2013-10-29. PMID:23860320. |
protein levels of factors in the insulin signaling pathway involved in glycogen synthesis were examined by western blot analysis, with the only significant change observed being the level of phosphorylated (at ser 9) glycogen synthase kinase-3β (gsk-3β) (p<0.001). |
2013-10-29 |
2023-08-12 |
mouse |
| Antonio J Ruiz-Alcaraz, Christopher Lipina, John R Petrie, Michael J Murphy, Andrew D Morris, Calum Sutherland, Daniel J Cuthbertso. Obesity-induced insulin resistance in human skeletal muscle is characterised by defective activation of p42/p44 MAP kinase. PloS one. vol 8. issue 2. 2013-08-23. PMID:23468892. |
skeletal muscle (vastus lateralis) biopsies were taken before and after one hour of hyperinsulinaemia and the muscle insulin signalling proteins examined by western blot and immunoprecipitation assay. |
2013-08-23 |
2023-08-12 |
human |
| Hongxia Zhang, Junjie Hou, Ruina Cui, Xuejiang Guo, Zhimin Shi, Fuquan Yang, Jiayin Da. Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. Toxicology letters. vol 218. issue 1. 2013-04-16. PMID:23353032. |
western blot and qpcr results suggested that chronic pfdoa exposure inhibited insulin signal pathways and that inhibition of gsk3 might contribute to the observed increases of lipid levels in the liver. |
2013-04-16 |
2023-08-12 |
rat |
| Yue-Chun Wang, Yuan Zhang, A-Lin Duan, Wei-Xia Lin, Qiao-Dan Zheng, Wen-Lu X. [Rapid differentiation of human umbilical cord-derived mesenchymal stem cells into insulin-secreting cells under the sole induction of biological products]. Sheng li xue bao : [Acta physiologica Sinica]. vol 62. issue 1. 2013-01-24. PMID:20179892. |
before and after the induction, the morphological changes were observed under inverse microscope; the islet-related genes were detected by rt-pcr; islet-like clusters (ilcs) were identified by dithizone (dtz) staining; pdx-1 and immunoreactive insulin (iri) were examined by immunofluorescence method; the quantity and quality of iri secretion were assayed by chemiluminescence immunoassay and western blot respectively. |
2013-01-24 |
2023-08-12 |
human |
| Li Wang, Wei Zhou, Shanmiao Gou, Tongling Wang, Tao Liu, Chunyou Wan. Insulin promotes proliferative vitality and invasive capability of pancreatic cancer cells via hypoxia-inducible factor 1alpha pathway. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 30. issue 3. 2013-01-24. PMID:20556580. |
western blot analysis demonstrated that insulin dose-dependently increased the hif-1alpha protein expression, and yc-1 could dose-dependently block this effect. |
2013-01-24 |
2023-08-12 |
human |