| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Min Jung Kim, Sabarinathan Rangasamy, Yumi Shim, Joon Myong Son. Cell lysis-free quantum dot multicolor cellular imaging-based mechanism study for TNF-α-induced insulin resistance. Journal of nanobiotechnology. vol 13. 2015-09-30. PMID:25623542. |
until now, many studies have been conducted to investigate the mechanism of tnf-α-induced insulin resistance based on western blot. |
2015-09-30 |
2023-08-13 |
Not clear |
| L A Winkelman, T R Overto. Long-acting insulins alter milk composition and metabolism of lactating dairy cows. Journal of dairy science. vol 96. issue 12. 2015-09-11. PMID:24119807. |
mammary biopsies were conducted on d 11, and mammary proteins extracted from the biopsies were analyzed by western blot for components of insulin and mammalian target of rapamycin signaling pathways. |
2015-09-11 |
2023-08-12 |
Not clear |
| Francisco José Ortega, Marta Serrano, Sergio Rodriguez-Cuenca, José María Moreno-Navarrete, María Gómez-Serrano, Mònica Sabater, Jose Ignacio Rodriguez-Hermosa, Gemma Xifra, Wifredo Ricart, Belén Peral, Antonio Vidal-Puig, José Manuel Fernández-Rea. Transducin-like enhancer of split 3 (TLE3) in adipose tissue is increased in situations characterized by decreased PPARγ gene expression. Journal of molecular medicine (Berlin, Germany). vol 93. issue 1. 2015-09-08. PMID:25249007. |
this study aimed to investigate tle3 gene expression (qrt-pcr) and protein (western blot) in subjects with a wide spectrum of obesity and insulin sensitivity and in an independent cohort of obese subjects following surgery-induced weight loss. |
2015-09-08 |
2023-08-13 |
mouse |
| Vishal M Balaramnavar, Rohit Srivastava, Neha Rahuja, Swati Gupta, Arun K Rawat, Salil Varshney, Hardik Chandasana, Yashpal S Chhonker, Pawan Kumar Doharey, Santosh Kumar, Sudeep Gautam, Swayam Prakash Srivastava, Rabi Sankar Bhatta, Jitendra Kumar Saxena, Anil Nilkanth Gaikwad, Arvind K Srivastava, Anil K Saxen. Identification of novel PTP1B inhibitors by pharmacophore based virtual screening, scaffold hopping and docking. European journal of medicinal chemistry. vol 87. 2015-08-19. PMID:25299681. |
western blot analysis of both skeletal muscle and liver demonstrated that proteins and intermediate enzymes of insulin signaling were also increased as compared to control group. |
2015-08-19 |
2023-08-13 |
mouse |
| Nathan D Roe, Emily Y He, Zhenbiao Wu, Jun Re. Folic acid reverses nitric oxide synthase uncoupling and prevents cardiac dysfunction in insulin resistance: role of Ca2+/calmodulin-activated protein kinase II. Free radical biology & medicine. vol 65. 2015-08-11. PMID:23820268. |
western blot analysis showed that insulin resistance significantly promoted ca(2+)/calmodulin-dependent protein kinase ii (camkii) phosphorylation, which might be responsible for the leaky ryr and cardiac mechanical dysfunction. |
2015-08-11 |
2023-08-12 |
mouse |
| Szu-Chuan Shen, Wen-Chang Chang, Chiao-Li Chan. Fraction from wax apple [Syzygium samarangense (Blume) Merrill and Perry] fruit extract ameliorates insulin resistance via modulating insulin signaling and inflammation pathway in tumor necrosis factor α-treated FL83B mouse hepatocytes. International journal of molecular sciences. vol 13. issue 7. 2015-08-07. PMID:22942720. |
western blot analysis revealed that, compared with the tnf-α-treated control group, fwfe increased the expression of the insulin receptor (ir), insulin receptor substrate-1 (irs-1), protein kinase b (akt/pkb), phosphatidylinositol-3 kinase (pi3k), and glucose transporter 2 (glut-2), and increased ir tyrosyl phosporylation, in insulin resistant fl83b cells. |
2015-08-07 |
2023-08-12 |
mouse |
| Fangfang Fang, Donglong Chen, Pan Yu, Wenyi Qian, Jing Zhou, Jingli Liu, Rong Gao, Jun Wang, Hang Xia. Effects of Bisphenol A on glucose homeostasis and brain insulin signaling pathways in male mice. General and comparative endocrinology. vol 212. 2015-08-04. PMID:25637671. |
the insulin levels were detected with radioimmunoassay (ria), and the insulin signaling pathways were investigated by western blot. |
2015-08-04 |
2023-08-13 |
mouse |
| Carolina De Ciuceis, Vincenzo Flati, Claudia Rossini, Anna Rufo, Enzo Porteri, Jacopo Di Gregorio, Beatrice Petroboni, Elisa La Boria, Carlotta Donini, Evasio Pasini, Enrico Agabiti Rosei, Damiano Rizzon. Effect of antihypertensive treatments on insulin signalling in lympho-monocytes of essential hypertensive patients: a pilot study. Blood pressure. vol 23. issue 6. 2015-07-01. PMID:24786779. |
insulin signalling was evaluated in mononuclear cells by isolating them through ficoll-paque density gradient centrifugation and protein analysis by western blot. |
2015-07-01 |
2023-08-13 |
human |
| Jing Wu, Hui-Ling Lu, Xiu-Fen Hu, Xiao-Yan Liang, Han-Hua Lin, Hong-Wei Wang, Xiao-Wei Fa. [Effect of acylation stimulating protein on the perilipin and adipophilin expression during 3T3-L1 preadipocyte differentiation.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 61. issue 1. 2015-05-16. PMID:19224055. |
the effects of asp or insulin on gene/protein expression of adipophilin and perilipin at indicated time points were evaluated by semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) and western blot. |
2015-05-16 |
2023-08-12 |
Not clear |
| Caleb W Grote, Anna L Groover, Janelle M Ryals, Paige C Geiger, Eva L Feldman, Douglas E Wrigh. Peripheral nervous system insulin resistance in ob/ob mice. Acta neuropathologica communications. vol 1. 2015-03-31. PMID:24252636. |
for these studies, nondiabetic control and type 2 diabetic ob/ob mice were challenged with an intrathecal injection of insulin or insulin-like growth factor 1 (igf-1) and downstream signaling was evaluated in the drg and sciatic nerve using western blot analysis. |
2015-03-31 |
2023-08-12 |
mouse |
| Rasoul Ghasemi, Asadollah Zarifkar, Karim Rastegar, Nader maghsoudi, Maryam Moosav. Insulin protects against Aβ-induced spatial memory impairment, hippocampal apoptosis and MAPKs signaling disruption. Neuropharmacology. vol 85. 2015-03-30. PMID:24881967. |
western blot analysis revealed that aβ25-35 induced elevation of caspase-3 and all 3 mapks subfamily activity, while insulin in 6 mu restored erk and p38 activation but has no effect on jnk. |
2015-03-30 |
2023-08-13 |
rat |
| Arefeh Jafarian, Mohammad Taghikhani, Saeid Abroun, Zahra Pourpak, Amir Allahverdi, Masoud Soleiman. Generation of high-yield insulin producing cells from human bone marrow mesenchymal stem cells. Molecular biology reports. vol 41. issue 7. 2015-02-02. PMID:24718781. |
western blot analysis discriminated insulin from proinsulin in the final differentiated cells. |
2015-02-02 |
2023-08-13 |
human |
| Irving L M H Aye, Susanne Lager, Vanessa I Ramirez, Francesca Gaccioli, Donald J Dudley, Thomas Jansson, Theresa L Powel. Increasing maternal body mass index is associated with systemic inflammation in the mother and the activation of distinct placental inflammatory pathways. Biology of reproduction. vol 90. issue 6. 2015-01-23. PMID:24759787. |
to determine the link between activated placental inflammatory pathways and elevated maternal cytokines, cultured primary human trophoblast (pht) cells were treated with physiological concentrations of insulin, mcp-1, and tnfalpha, and inflammatory signaling analyzed by western blot. |
2015-01-23 |
2023-08-13 |
human |
| An Song, Chao Wang, Luping Ren, Jiajun Zha. Swimming improves high-fat induced insulin resistance by regulating lipid and energy metabolism and the insulin pathway in rats. International journal of molecular medicine. vol 33. issue 6. 2014-12-18. PMID:24715199. |
quantitative rt-pcr and western blot analysis were performed to detect the expression of proteins related to lipid metabolism, energy metabolism and insulin signaling transduction. |
2014-12-18 |
2023-08-13 |
rat |
| Luiz F Rezende, Rafael L Camargo, Renato C S Branco, Ana P G Cappelli, Antonio C Boschero, Everardo M Carneir. Reduced insulin clearance and lower insulin-degrading enzyme expression in the liver might contribute to the thrifty phenotype of protein-restricted mice. The British journal of nutrition. vol 112. issue 6. 2014-11-24. PMID:25036874. |
insulin concentrations were determined using ria and protein expression and phosphorylation by western blot analysis. |
2014-11-24 |
2023-08-13 |
mouse |
| Jingbo Liu, Jian He, Yuekui Yang, Jie Yu, Xiangbing Mao, Bing Yu, Daiwen Che. Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs. Archives of animal nutrition. vol 68. issue 2. 2014-11-17. PMID:24646150. |
western blot analysis indicated that hf feeding elevated the protein expressions of tlr4 signalling pathway, as tlr4, myd88, iκb kinase β and nuclear factor-κb, and insulin signalling-related proteins, as phosphorylated insulin receptor substrate-1 and phosphorylated protein kinase b. |
2014-11-17 |
2023-08-12 |
Not clear |
| Michelle Bamji-Mirza, Debbie Callaghan, Dema Najem, Shanshan Shen, Mohamed Shaad Hasim, Ze Yang, Wandong Zhan. Stimulation of insulin signaling and inhibition of JNK-AP1 activation protect cells from amyloid-β-induced signaling dysregulation and inflammatory response. Journal of Alzheimer's disease : JAD. vol 40. issue 1. 2014-10-30. PMID:24346217. |
transcription factor profiling showed that treatment of ihbec with aβ1-42, insulin, or sp600125 alone or in combination resulted in profound changes in modulating the activities of multiple transcription factors and relevant pathways, some of which were validated by western blot. |
2014-10-30 |
2023-08-12 |
human |
| Y Hu, X-H Xu, K He, L-L Zhang, S-K Wang, Y-Q Pan, B-S He, T-T Feng, X-M Ma. Genome-wide analysis of DNA methylation variations caused by chronic glucolipotoxicity in beta-cells. Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association. vol 122. issue 2. 2014-10-15. PMID:24554506. |
the mrna and protein expression of insulin and tcf7l2 in these cells were quantified by rt-pcr and western blot, respectively.we have identified a lot of loci which experienced aberrant dna methylation in beta-cells cultured with high-glucose-lipid medium. |
2014-10-15 |
2023-08-12 |
Not clear |
| Yun-A Jung, Hee Kyoung Kim, Kwi-Hyun Bae, Hye-Young Seo, Hye-Soon Kim, Byoung Kuk Jang, Gwon-Soo Jung, In-Kyu Lee, Mi-Kyung Kim, Keun-Gyu Par. Cilostazol inhibits insulin-stimulated expression of sterol regulatory binding protein-1c via inhibition of LXR and Sp1. Experimental & molecular medicine. vol 46. 2014-09-25. PMID:24458133. |
the effects of cilostazol on the expression of srebp-1c and its target genes in response to insulin or an lxr agonist (t0901317) were examined using real-time rt-pcr and western blot analysis on cultured hepatocytes. |
2014-09-25 |
2023-08-12 |
Not clear |
| So Yeon Park, Min Hye Kim, Joung Hoon Ahn, Su Jin Lee, Jong Ho Lee, Won Sik Eum, Soo Young Choi, Hyeok Yil Kwo. The Stimulatory Effect of Essential Fatty Acids on Glucose Uptake Involves Both Akt and AMPK Activation in C2C12 Skeletal Muscle Cells. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology. vol 18. issue 3. 2014-06-30. PMID:24976766. |
two efas and oa significantly protected pa-induced suppression of insulin signaling, respectively, which was confirmed by the increased levels of akt phosphorylation and serine/threonine kinases (pkcθ and jnk) dephosphorylation in the western blot analysis. |
2014-06-30 |
2023-08-13 |
Not clear |