| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ruiyi Yang, Lu Wang, Jie Xie, Xiang Li, Shan Liu, Shengxiang Qiu, Yingjie Hu, Xiaoling She. Treatment of type 2 diabetes mellitus via reversing insulin resistance and regulating lipid homeostasis in vitro and in vivo using cajanonic acid A. International journal of molecular medicine. vol 42. issue 5. 2019-01-21. PMID:30226559. |
the expression levels of insulin signaling factors were detected via reverse transcription‑quantitative polymerase chain reaction and western blot analyses. |
2019-01-21 |
2023-08-13 |
mouse |
| Shuyi Wang, Xiyao Chen, Sreejayan Nair, Dongdong Sun, Xiaoming Wang, Jun Re. Deletion of protein tyrosine phosphatase 1B obliterates endoplasmic reticulum stress-induced myocardial dysfunction through regulation of autophagy. Biochimica et biophysica acta. Molecular basis of disease. vol 1863. issue 12. 2018-12-11. PMID:28941626. |
western blot analysis was used to monitor the levels of er stress, autophagy and insulin signaling including insulin receptor substrate (irs), tribbles homolog 3 (trib3), atg5/7, p62 and lc3-ii. |
2018-12-11 |
2023-08-13 |
mouse |
| Shingo Ito, Mari Yanai, Shunsuke Yamaguchi, Pierre-Olivier Couraud, Sumio Ohtsuk. Regulation of Tight-Junction Integrity by Insulin in an In Vitro Model of Human Blood-Brain Barrier. Journal of pharmaceutical sciences. vol 106. issue 9. 2018-11-09. PMID:28456720. |
western blot analysis showed that insulin induced phosphorylation of extracellular signal-regulated kinase and insulin receptor substrate-1 in hcmec/d3 cells. |
2018-11-09 |
2023-08-13 |
human |
| Fang Chen, Yingkun He, Pengwen Wang, Peng Wei, Huili Feng, Yingxue Rao, Jing Shi, Jinzhou Tia. Banxia Xiexin decoction ameliorated cognition via the regulation of insulin pathways and glucose transporters in the hippocampus of APPswe/PS1dE9 mice. International journal of immunopathology and pharmacology. vol 32. 2018-10-31. PMID:29873261. |
proteins involved in insulin signalling pathways and glucose transports in the hippocampus were assessed through immunohistochemical staining and western blot. |
2018-10-31 |
2023-08-13 |
mouse |
| Kenneth T Lewis, Akshata R Naik, Suvra S Laha, Sunxi Wang, Guangzhao Mao, Eric Kuhn, Bhanu P Jen. Secretion induces cell pH dynamics impacting assembly-disassembly of the fusion protein complex: A combined fluorescence and atomic force microscopy study. Seminars in cell & developmental biology. vol 73. 2018-10-18. PMID:28779980. |
in the current study, a novel approach using cadmium telluride quantum dots (cdteqds) as ph sensors, combined with fluorescent imaging, spectrofluorimetry, atomic force microscopy (afm), and western blot analysis, enabled the study of intracellular ph dynamics at 1 milli-ph sensitivity and 80nm pixel resolution, during insulin secretion. |
2018-10-18 |
2023-08-13 |
mouse |
| Moon Ho Do, Eunjung Lee, Mi-Jin Oh, Yoonsook Kim, Ho-Young Par. High-Glucose or -Fructose Diet Cause Changes of the Gut Microbiota and Metabolic Disorders in Mice without Body Weight Change. Nutrients. vol 10. issue 6. 2018-10-15. PMID:29899272. |
gut microbiota-derived endotoxin-induced metabolic disorders were evaluated by glucose and insulin tolerance test, gut permeability, western blot and histological analysis. |
2018-10-15 |
2023-08-13 |
mouse |
| Ke-Ke Jia, Shu-Man Pan, Hong Ding, Jia-Hui Liu, Yan-Jing Zheng, Shui-Juan Wang, Ying Pan, Ling-Dong Kon. Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 103. 2018-10-11. PMID:29864926. |
western blot was used to detect the expression of key proteins in inflammatory signaling cascades including toll-like receptor 4 (tlr4), myeloid differentiation protein 88 (myd88), nuclear factor-kappa b (nf-κb), nod-like receptor family pyrin domain containing 3 (nlrp3), apoptosis-associated speck-like protein containing card (asc), cysteinyl aspartate specific proteinase-1 (caspase-1) and il-1β, as well as insulin signaling in liver and prefrontal cortex of rats. |
2018-10-11 |
2023-08-13 |
rat |
| Ying Xin, Haiyan Zhang, Zhaotong Jia, Xiaoqian Ding, Yong Sun, Qiang Wang, Tao X. Resveratrol improves uric acid-induced pancreatic β-cells injury and dysfunction through regulation of miR-126. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 102. 2018-10-02. PMID:29710530. |
then, cell viability, apoptosis, apoptosis-associated factors, inos expression and insulin secretion were examined by cck-8, flow cytometry, western blot, qrt-pcr and glucose-stimulated insulin secretion (gsis), respectively. |
2018-10-02 |
2023-08-13 |
mouse |
| Yang Wang, Ri-Bao Wei, Yue Yang, Ting-Yu Su, Meng-Jie Huang, Ping Li, Xiang-Mei Che. Valsartan Alleviates Insulin Resistance in Skeletal Muscle of Chronic Renal Failure Rats. Medical science monitor : international medical journal of experimental and clinical research. vol 24. 2018-09-28. PMID:29679000. |
western blot assays were performed to detect the expression and phosphorylation of irs-1 and akt, which are 2 critical proteins in the insulin signaling pathway. |
2018-09-28 |
2023-08-13 |
rat |
| Dalia H Abdelkader, Mohamed A Osman, Sanaa A El-Gizawy, Susan J Hawthorne, Ahmed M Faheem, Paul A McCarro. Effect of poly(ethylene glycol) on insulin stability and cutaneous cell proliferation in vitro following cytoplasmic delivery of insulin-loaded nanoparticulate carriers - A potential topical wound management approach. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. vol 114. 2018-08-27. PMID:29288081. |
recombinant human insulin was encapsulated in poly(d,l-lactide-co-glycolide) nanoparticles, manufactured with variation in poly(ethylene glycol) content, and shown to be stable for 6days using sds-page, western blot and maldi ms. to determine if insulin released from this sustained release matrix could stimulate migration of cell types normally active in dermal repair, a model wound was simulated by scratching confluent cultures of human keratinocytes (hacat) and fibroblasts (hs27). |
2018-08-27 |
2023-08-13 |
human |
| Caijuan Wang, Yujiao Deng, Yenan Yue, Wenting Chen, Yu Zhang, Guifang Shi, Zhongming W. Glutamine Enhances the Hypoglycemic Effect of Insulin in L6 Cells via Phosphatidylinositol-3-Kinase (PI3K)/Protein Kinase B (AKT)/Glucose Transporter 4 (GLUT4) Signaling Pathway. Medical science monitor : international medical journal of experimental and clinical research. vol 24. 2018-08-27. PMID:29491345. |
the expression of insulin signaling molecules, including phosphatidylinositol-3-kinase (pi3k), 3-phosphoinositide-dependent protein kinase-1 (pdk1), protein kinase b (akt), protein kinase c zeta (pkcz), and glucose transporter 4 (glut4), were detected by real-time pcr and western blot analysis, glut4 translocation was observed by immunofluorescence staining, glycogen synthase kinase (gsk) was analyzed by western blotting, and glucose uptake was measured by glucose oxidase method (god). |
2018-08-27 |
2023-08-13 |
Not clear |
| Dongzhi Hu, Yi Wang, Haiyang Zhang, Dalu Kon. Identification of miR-9 as a negative factor of insulin secretion from beta cells. Journal of physiology and biochemistry. vol 74. issue 2. 2018-08-17. PMID:29470815. |
concentration of insulin was determined by elisa, and the expression of the target protein was determined with western blot assay. |
2018-08-17 |
2023-08-13 |
mouse |
| Hao Sun, Jun Cai, Li Xu, Jiamei Liu, Mulei Chen, Meili Zheng, Lefeng Wang, Xinchun Yan. miR-483-3p regulates acute myocardial infarction by transcriptionally repressing insulin growth factor 1 expression. Molecular medicine reports. vol 17. issue 3. 2018-08-14. PMID:29363719. |
b‑cell lymphoma 2 (bcl‑2)/bcl‑2‑associated x protein (bax) and transcriptionally suppressed the protein expression of insulin growth factor 1 (igf‑1) were analyze using western blot analysis. |
2018-08-14 |
2023-08-13 |
Not clear |
| Yuan-Chiang Chung, Chung-Yuh Tzeng, Ying-I Chen, Shu-Wei Chang, Tai-Hao Hsu, Wai-Jane Ho, Yueh-Hsiung Kuo, Pei-Hsiu Hung, Shih-Liang Chan. Improving insulin resistance with Antrodia cinnamomea mycelium powder to induce a hypoglycemic effect in dexamethasone‑induced insulin‑resistant rats. Molecular medicine reports. vol 17. issue 2. 2018-08-03. PMID:29257321. |
changes in plasma ffa levels were detected and levels of insulin signal proteins (irs‑1, glut‑4 and pi3k) were analyzed by western blot to elucidate ac's mechanism of action. |
2018-08-03 |
2023-08-13 |
rat |
| Krzysztof Marycz, Katarzyna Kornicka, Jolanta Szlapka-Kosarzewska, Christine Weis. Excessive Endoplasmic Reticulum Stress Correlates with Impaired Mitochondrial Dynamics, Mitophagy and Apoptosis, in Liver and Adipose Tissue, but Not in Muscles in EMS Horses. International journal of molecular sciences. vol 19. issue 1. 2018-07-26. PMID:29316632. |
we established the expression of genes related to insulin resistance, endoplasmic reticulum (er) stress and mitochondria clearance by mitophagy using rt-pcr and western blot. |
2018-07-26 |
2023-08-13 |
human |
| Rubí Viedma-Rodríguez, María Guadalupe Martínez-Hernández, Luis Antonio Flores-López, Luis Arturo Baiza-Gutma. Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system. Molecular and cellular biochemistry. vol 437. issue 1-2. 2018-07-09. PMID:28612231. |
mda-mb-231 cells were treated with eaca 12.5 mmol/l under high glucose 30 mmol/l (hg) and high glucose and insulin 80 nmol/l (hg-i) conditions, evaluating: cell population growth, % of viability, migratory, and invasive abilities, as well as the expression of upa, its receptor (upar), and its inhibitor (pai-1), by real-time reverse transcription-polymerase chain reaction (rt-pcr) and western blot, mmp-2 and mmp-9 mrnas were evaluated by rt-pcr. |
2018-07-09 |
2023-08-13 |
Not clear |
| Santosh K Mandal, Prem K Shrestha, Fahaad S H Alenazi, Manita Shakya, Hussain Alhamami, Karen P Brisk. Role of hindbrain adenosine 5'-monophosphate-activated protein kinase (AMPK) in hypothalamic AMPK and metabolic neuropeptide adaptation to recurring insulin-induced hypoglycemia in the male rat. Neuropeptides. vol 66. 2018-06-27. PMID:28823463. |
brain tissue was harvested after single versus serial insulin (i) dosing for western blot analysis of ampk, phospho-ampk (pampk), and relevant biosynthetic enzyme/neuropeptide expression in micro-punch dissected arcuate (arh), ventromedial (vmh), dorsomedial (dmh) nuclei and lateral hypothalamic area (lha) tissue. |
2018-06-27 |
2023-08-13 |
rat |
| Fan Wang, Shaobing Wang, Zhenghong Zhang, Qingqiang Lin, Yiping Liu, Yijun Xiao, Kaizhuan Xiao, Zhengchao Wan. Defective insulin signaling and the protective effects of dimethyldiguanide during follicular development in the ovaries of polycystic ovary syndrome. Molecular medicine reports. vol 16. issue 6. 2018-06-25. PMID:28990055. |
subsequently, the expression of two key elements of insulin signaling, insulin receptor substrate (irs)‑2 and phosphatidylinositol 3‑kinase (pi3k), was determined by immunohistochemistry and western blot analysis. |
2018-06-25 |
2023-08-13 |
rat |
| Mei-Lin Wei, Peng Duan, Zhi-Ming Wang, Miao Ding, Ping T. High glucose and high insulin conditions promote MCF‑7 cell proliferation and invasion by upregulating IRS1 and activating the Ras/Raf/ERK pathway. Molecular medicine reports. vol 16. issue 5. 2018-06-04. PMID:28901503. |
reverse transcription‑quantitative polymerase chain reaction and western blot analyses revealed that insulin receptor substrate 1 (irs1) was significantly upregulated following high glucose and high insulin treatment compared with normal glucose and low insulin conditions. |
2018-06-04 |
2023-08-13 |
Not clear |
| Meihong Xu, Bin Sun, Di Li, Ruixue Mao, Hui Li, Yong Li, Junbo Wan. Beneficial Effects of Small Molecule Oligopeptides Isolated from Panax ginseng Meyer on Pancreatic Beta-Cell Dysfunction and Death in Diabetic Rats. Nutrients. vol 9. issue 10. 2018-06-04. PMID:28954411. |
the insulin level and the expression of nf-κb and bcl-2 family in pancreatic islets were also detected by western blot analysis. |
2018-06-04 |
2023-08-13 |
rat |