| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ke-Ke Jia, Shu-Man Pan, Hong Ding, Jia-Hui Liu, Yan-Jing Zheng, Shui-Juan Wang, Ying Pan, Ling-Dong Kon. Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 103. 2018-10-11. PMID:29864926. |
western blot was used to detect the expression of key proteins in inflammatory signaling cascades including toll-like receptor 4 (tlr4), myeloid differentiation protein 88 (myd88), nuclear factor-kappa b (nf-κb), nod-like receptor family pyrin domain containing 3 (nlrp3), apoptosis-associated speck-like protein containing card (asc), cysteinyl aspartate specific proteinase-1 (caspase-1) and il-1β, as well as insulin signaling in liver and prefrontal cortex of rats. |
2018-10-11 |
2023-08-13 |
rat |
| Ying Xin, Haiyan Zhang, Zhaotong Jia, Xiaoqian Ding, Yong Sun, Qiang Wang, Tao X. Resveratrol improves uric acid-induced pancreatic β-cells injury and dysfunction through regulation of miR-126. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 102. 2018-10-02. PMID:29710530. |
then, cell viability, apoptosis, apoptosis-associated factors, inos expression and insulin secretion were examined by cck-8, flow cytometry, western blot, qrt-pcr and glucose-stimulated insulin secretion (gsis), respectively. |
2018-10-02 |
2023-08-13 |
mouse |
| Yang Wang, Ri-Bao Wei, Yue Yang, Ting-Yu Su, Meng-Jie Huang, Ping Li, Xiang-Mei Che. Valsartan Alleviates Insulin Resistance in Skeletal Muscle of Chronic Renal Failure Rats. Medical science monitor : international medical journal of experimental and clinical research. vol 24. 2018-09-28. PMID:29679000. |
western blot assays were performed to detect the expression and phosphorylation of irs-1 and akt, which are 2 critical proteins in the insulin signaling pathway. |
2018-09-28 |
2023-08-13 |
rat |
| Dalia H Abdelkader, Mohamed A Osman, Sanaa A El-Gizawy, Susan J Hawthorne, Ahmed M Faheem, Paul A McCarro. Effect of poly(ethylene glycol) on insulin stability and cutaneous cell proliferation in vitro following cytoplasmic delivery of insulin-loaded nanoparticulate carriers - A potential topical wound management approach. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. vol 114. 2018-08-27. PMID:29288081. |
recombinant human insulin was encapsulated in poly(d,l-lactide-co-glycolide) nanoparticles, manufactured with variation in poly(ethylene glycol) content, and shown to be stable for 6days using sds-page, western blot and maldi ms. to determine if insulin released from this sustained release matrix could stimulate migration of cell types normally active in dermal repair, a model wound was simulated by scratching confluent cultures of human keratinocytes (hacat) and fibroblasts (hs27). |
2018-08-27 |
2023-08-13 |
human |
| Caijuan Wang, Yujiao Deng, Yenan Yue, Wenting Chen, Yu Zhang, Guifang Shi, Zhongming W. Glutamine Enhances the Hypoglycemic Effect of Insulin in L6 Cells via Phosphatidylinositol-3-Kinase (PI3K)/Protein Kinase B (AKT)/Glucose Transporter 4 (GLUT4) Signaling Pathway. Medical science monitor : international medical journal of experimental and clinical research. vol 24. 2018-08-27. PMID:29491345. |
the expression of insulin signaling molecules, including phosphatidylinositol-3-kinase (pi3k), 3-phosphoinositide-dependent protein kinase-1 (pdk1), protein kinase b (akt), protein kinase c zeta (pkcz), and glucose transporter 4 (glut4), were detected by real-time pcr and western blot analysis, glut4 translocation was observed by immunofluorescence staining, glycogen synthase kinase (gsk) was analyzed by western blotting, and glucose uptake was measured by glucose oxidase method (god). |
2018-08-27 |
2023-08-13 |
Not clear |
| Dongzhi Hu, Yi Wang, Haiyang Zhang, Dalu Kon. Identification of miR-9 as a negative factor of insulin secretion from beta cells. Journal of physiology and biochemistry. vol 74. issue 2. 2018-08-17. PMID:29470815. |
concentration of insulin was determined by elisa, and the expression of the target protein was determined with western blot assay. |
2018-08-17 |
2023-08-13 |
mouse |
| Hao Sun, Jun Cai, Li Xu, Jiamei Liu, Mulei Chen, Meili Zheng, Lefeng Wang, Xinchun Yan. miR-483-3p regulates acute myocardial infarction by transcriptionally repressing insulin growth factor 1 expression. Molecular medicine reports. vol 17. issue 3. 2018-08-14. PMID:29363719. |
b‑cell lymphoma 2 (bcl‑2)/bcl‑2‑associated x protein (bax) and transcriptionally suppressed the protein expression of insulin growth factor 1 (igf‑1) were analyze using western blot analysis. |
2018-08-14 |
2023-08-13 |
Not clear |
| Yuan-Chiang Chung, Chung-Yuh Tzeng, Ying-I Chen, Shu-Wei Chang, Tai-Hao Hsu, Wai-Jane Ho, Yueh-Hsiung Kuo, Pei-Hsiu Hung, Shih-Liang Chan. Improving insulin resistance with Antrodia cinnamomea mycelium powder to induce a hypoglycemic effect in dexamethasone‑induced insulin‑resistant rats. Molecular medicine reports. vol 17. issue 2. 2018-08-03. PMID:29257321. |
changes in plasma ffa levels were detected and levels of insulin signal proteins (irs‑1, glut‑4 and pi3k) were analyzed by western blot to elucidate ac's mechanism of action. |
2018-08-03 |
2023-08-13 |
rat |
| Krzysztof Marycz, Katarzyna Kornicka, Jolanta Szlapka-Kosarzewska, Christine Weis. Excessive Endoplasmic Reticulum Stress Correlates with Impaired Mitochondrial Dynamics, Mitophagy and Apoptosis, in Liver and Adipose Tissue, but Not in Muscles in EMS Horses. International journal of molecular sciences. vol 19. issue 1. 2018-07-26. PMID:29316632. |
we established the expression of genes related to insulin resistance, endoplasmic reticulum (er) stress and mitochondria clearance by mitophagy using rt-pcr and western blot. |
2018-07-26 |
2023-08-13 |
human |
| Rubí Viedma-Rodríguez, María Guadalupe Martínez-Hernández, Luis Antonio Flores-López, Luis Arturo Baiza-Gutma. Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system. Molecular and cellular biochemistry. vol 437. issue 1-2. 2018-07-09. PMID:28612231. |
mda-mb-231 cells were treated with eaca 12.5 mmol/l under high glucose 30 mmol/l (hg) and high glucose and insulin 80 nmol/l (hg-i) conditions, evaluating: cell population growth, % of viability, migratory, and invasive abilities, as well as the expression of upa, its receptor (upar), and its inhibitor (pai-1), by real-time reverse transcription-polymerase chain reaction (rt-pcr) and western blot, mmp-2 and mmp-9 mrnas were evaluated by rt-pcr. |
2018-07-09 |
2023-08-13 |
Not clear |
| Santosh K Mandal, Prem K Shrestha, Fahaad S H Alenazi, Manita Shakya, Hussain Alhamami, Karen P Brisk. Role of hindbrain adenosine 5'-monophosphate-activated protein kinase (AMPK) in hypothalamic AMPK and metabolic neuropeptide adaptation to recurring insulin-induced hypoglycemia in the male rat. Neuropeptides. vol 66. 2018-06-27. PMID:28823463. |
brain tissue was harvested after single versus serial insulin (i) dosing for western blot analysis of ampk, phospho-ampk (pampk), and relevant biosynthetic enzyme/neuropeptide expression in micro-punch dissected arcuate (arh), ventromedial (vmh), dorsomedial (dmh) nuclei and lateral hypothalamic area (lha) tissue. |
2018-06-27 |
2023-08-13 |
rat |
| Fan Wang, Shaobing Wang, Zhenghong Zhang, Qingqiang Lin, Yiping Liu, Yijun Xiao, Kaizhuan Xiao, Zhengchao Wan. Defective insulin signaling and the protective effects of dimethyldiguanide during follicular development in the ovaries of polycystic ovary syndrome. Molecular medicine reports. vol 16. issue 6. 2018-06-25. PMID:28990055. |
subsequently, the expression of two key elements of insulin signaling, insulin receptor substrate (irs)‑2 and phosphatidylinositol 3‑kinase (pi3k), was determined by immunohistochemistry and western blot analysis. |
2018-06-25 |
2023-08-13 |
rat |
| Mei-Lin Wei, Peng Duan, Zhi-Ming Wang, Miao Ding, Ping T. High glucose and high insulin conditions promote MCF‑7 cell proliferation and invasion by upregulating IRS1 and activating the Ras/Raf/ERK pathway. Molecular medicine reports. vol 16. issue 5. 2018-06-04. PMID:28901503. |
reverse transcription‑quantitative polymerase chain reaction and western blot analyses revealed that insulin receptor substrate 1 (irs1) was significantly upregulated following high glucose and high insulin treatment compared with normal glucose and low insulin conditions. |
2018-06-04 |
2023-08-13 |
Not clear |
| Meihong Xu, Bin Sun, Di Li, Ruixue Mao, Hui Li, Yong Li, Junbo Wan. Beneficial Effects of Small Molecule Oligopeptides Isolated from Panax ginseng Meyer on Pancreatic Beta-Cell Dysfunction and Death in Diabetic Rats. Nutrients. vol 9. issue 10. 2018-06-04. PMID:28954411. |
the insulin level and the expression of nf-κb and bcl-2 family in pancreatic islets were also detected by western blot analysis. |
2018-06-04 |
2023-08-13 |
rat |
| Yan Su, Juan Wu, Junlin He, Xueqing Liu, Xuemei Chen, Yubin Ding, Chen Zhang, Wenqi Chen, Yingxiong Wang, Rufei Ga. High insulin impaired ovarian function in early pregnant mice and the role of autophagy in this process. Endocrine journal. vol 64. issue 6. 2018-05-30. PMID:28420820. |
western blot analysis showed that the expressions of lc3 (microtubule-associated protein 1 light chain 3), becn1, and cathepsin b proteins in ovaries from insulin group were obviously reduced, while p62 proteins were significantly increased. |
2018-05-30 |
2023-08-13 |
mouse |
| Huiwen Ma, Ping Wang, Dan Jin, Ting Jia, Hong Mao, Jiandi Zhang, Shi Zha. The hepatic ectonucleotide pyrophosphatase/phosphodiesterase 1 gene mRNA abundance is reduced by insulin and induced by dexamethasone. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 51. issue 4. 2018-05-24. PMID:29513794. |
to determine its potential role in the regulation of metabolism, we analyzed its gene (enpp1) expression in the liver of rats experiencing fasting and refeeding cycles, and in primary rat hepatocytes and human hepatoma hepg2 cells treated with insulin and dexamethasone using northern blot and real-time pcr techniques. |
2018-05-24 |
2023-08-13 |
human |
| Sandeep M S, Nandini C . Influence of quercetin, naringenin and berberine on glucose transporters and insulin signalling molecules in brain of streptozotocin-induced diabetic rats. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 94. 2018-05-15. PMID:28783583. |
in the present study, we looked into the effect of them on expression of various glucose transporters and key components of brain insulin signalling, namely, insulin receptor substrate 1 (irs 1), phosphatidyl inositol 3 kinase (pi3k), akt 1 and low-density lipoprotein receptor-related protein 1 (lrp1) in brain of control, diabetic and bioactive-treated rats by western blot. |
2018-05-15 |
2023-08-13 |
rat |
| Jong Seok Lee, Jun Myoung Park, Sarah Lee, Hye Jin Lee, Hee-Sun Yang, Joohong Yeo, Ki Rim Lee, Byung Hyun Choi, Eock Kee Hon. Hispidin rescues palmitate‑induced insulin resistance in C2C12 myotubes. Molecular medicine reports. vol 16. issue 4. 2018-05-14. PMID:28731188. |
the present study investigated the effects of hispidin on palmitate‑induced insulin resistance in c2c12 skeletal muscle myotubes via an mtt assay, glucose uptake assay, oil‑red‑o staining and western blot analysis. |
2018-05-14 |
2023-08-13 |
Not clear |
| Tamara B Dakic, Tanja V Jevdjovic, Mina I Peric, Ivana M Bjelobaba, Milica B Markelic, Bojana S Milutinovic, Iva V Lakic, Nebojsa I Jasnic, Jelena D Djordjevic, Predrag Z Vujovi. Short-term fasting promotes insulin expression in rat hypothalamus. The European journal of neuroscience. vol 46. issue 1. 2018-03-26. PMID:28544147. |
hypothalamic insulin content was assessed by both radioimmunoassay and western blot. |
2018-03-26 |
2023-08-13 |
rat |
| Long Cheng, Junmei Song, Geng Li, Yue Liu, Yuming Wang, Xiangbao Meng, Guibo Sun, Xiaobo Su. Effects of the Tangningtongluo formula as an alternative strategy for diabetics via upregulation of insulin receptor substrate-1. Molecular medicine reports. vol 16. issue 1. 2018-03-26. PMID:28586073. |
western blot analysis indicated that upregulation of phosphorylated insulin receptor substrate‑1 (p‑irs‑1) by the administration of tntl may be strongly involved in the improvement of insulin resistance. |
2018-03-26 |
2023-08-13 |
mouse |