| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Leilei Yu, Huifeng Wang, Zhongxiu Guo, Fenghua Li, Hong Cu. Role of nucleolar protein NOM1 in pancreatic islet β cell apoptosis in diabetes. Experimental and therapeutic medicine. vol 12. issue 4. 2020-10-01. PMID:27698723. |
furthermore, the mrna expression levels of insulin 1 and 2 in min6 cells were detected using reverse transcription-quantitative polymerase chain reaction, while the expression levels of various cell apoptosis-associated proteins, bcl-2 and bax, were analyzed using western blot analysis. |
2020-10-01 |
2023-08-13 |
mouse |
| Huicun Zhang, Na Ta, Pengmin Chen, Hongbing Wan. Erchen Decoction and Linguizhugan Decoction Ameliorate Hepatic Insulin Resistance by Inhibiting IRS-1Ser307 Phosphorylation In Vivo and In Vitro. Evidence-based complementary and alternative medicine : eCAM. vol 2017. 2020-10-01. PMID:28630632. |
because insulin resistance (ir) is a key etiological factor in the pathology of high-fat diet- (hfd-) induced nafld, in this study, the protective effects of ecd and lgzgd on hfd-induced insulin resistance in rats were evaluated and their mechanisms were investigated by ogtt and western blot. |
2020-10-01 |
2023-08-13 |
rat |
| Ben Niu, Chao Li, Heng Su, Qingzhu Li, Qiu He, Lijuan Liu, Yuanming Xue, Tao Shen, Xueshan Xi. Glucagon-like peptide-1 receptor agonist exendin-4 protects against interleukin-1β-mediated inhibition of glucose-stimulated insulin secretion by mouse insulinoma β cells. Experimental and therapeutic medicine. vol 14. issue 3. 2020-10-01. PMID:28947919. |
cells were stimulated with various concentrations of glucose (0, 1.38, 5.5 and 11.1 mm), and insulin was measured via radioimmunoassay of the supernatant; furthermore, western blot analysis was used to detect phosphorylated extracellular receptor kinase (erk)1/2. |
2020-10-01 |
2023-08-13 |
mouse |
| Naren Tuya, Yadi Wang, Lanmei Tong, Weishi Gao, Rong Yu, Liying Xu. Trichosanthin enhances the antitumor effect of gemcitabine in non-small cell lung cancer via inhibition of the PI3K/AKT pathway. Experimental and therapeutic medicine. vol 14. issue 6. 2020-10-01. PMID:29285119. |
in addition, western blot analysis demonstrated that the combination treatment of tcs with gemz further decreased the expression level of phosphoinositide 3-kinase (pi3k) and akt via regulating the expression of insulin growth factor. |
2020-10-01 |
2023-08-13 |
Not clear |
| Wen Pan, Yanan Zhang, Chun Zeng, Fen Xu, Jinhua Yan, Jianping Wen. miR-192 is upregulated in T1DM, regulates pancreatic β-cell development and inhibits insulin secretion through suppressing GLP-1 expression. Experimental and therapeutic medicine. vol 16. issue 3. 2020-10-01. PMID:30186503. |
western blot and reverse transcription-quantitative polymerase chain reaction analysis indicated that mir-192 suppressed the expression of glucagon-like peptide-1 (glp-1), a potent insulin secretagogue. |
2020-10-01 |
2023-08-13 |
rat |
| Mohamad Hasannejad, Seyed Ziaaldin Samsamshariat, Armita Esmaili, Ali Jahanian-Najafabad. Klotho induces insulin resistance possibly through interference with GLUT4 translocation and activation of Akt, GSK3β, and PFKfβ3 in 3T3-L1 adipocyte cells. Research in pharmaceutical sciences. vol 14. issue 4. 2020-10-01. PMID:31516514. |
p-akt/akt, p-gsk3β/gsk3β, and p-pfkfβ3/pfkfβ3 ratios were determined in insulin and klotho/insulin treated cells using western blot. |
2020-10-01 |
2023-08-13 |
Not clear |
| Xin-Yu Huang, Liang Zhang, Jian Sun, Neng-Gui Xu, Wei Y. Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats. Evidence-based complementary and alternative medicine : eCAM. vol 2016. 2020-09-30. PMID:27738449. |
protein and mrna expressions of insulin signaling molecules were determined by western blot and real-time rt-pcr, respectively. |
2020-09-30 |
2023-08-13 |
rat |
| Xiao Wei, Dan Zhu, Chenchen Feng, Guofang Chen, Xiaodong Mao, Qifeng Wang, Jie Wang, Chao Li. Inhibition of peptidyl-prolyl cis-trans isomerase B mediates cyclosporin A-induced apoptosis of islet β cells. Experimental and therapeutic medicine. vol 16. issue 5. 2020-09-29. PMID:30344674. |
expression levels of insulin, peptidyl-prolyl cis-trans isomerase b (ppib), cleaved caspase-3, phospho-protein kinase r (pkr)-like endoplasmic reticulum kinase (p-perk), pkr-like endoplasmic reticulum kinase (perk), binding immunoglobulin protein (bip), and c/ebp homologous protein (chop) were detected via reducing western blot assay. |
2020-09-29 |
2023-08-13 |
Not clear |
| Changjun Yun, Wenjie Qian, Junyi Wu, Chenxi Yuan, Songzhou Jiang, Jinpeng L. Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway. Experimental and therapeutic medicine. vol 19. issue 2. 2020-09-28. PMID:32010253. |
to determine whether pap can promote osteoblast development and to elucidate the molecular mechanisms underlying its functions, the present study investigated the effects of pap on osteoblast proliferation, differentiation and mineralization, and the role of the insulin signaling pathway using mtt assay, alkaline phosphatase activity assay, western blot analysis and reverse transcription-quantitative pcr. |
2020-09-28 |
2023-08-13 |
Not clear |
| Shivasadat Gheflat, Abdolrahim Sadeghi, Mojgan Bandehpour, Keyvan Ramezani, Bahram Kazem. Designing an Engineered Construct Gene Sensitive to Carbohydrate In-vitro and Candidate for Human Insulin Gene Therapy In-vivo. Iranian journal of pharmaceutical research : IJPR. vol 18. issue 4. 2020-09-28. PMID:32184874. |
insulin expression in the transfected cells with 200 and 400 ng of the construct in comparison with negative control was detected using western blot and elisa methods. |
2020-09-28 |
2023-08-13 |
human |
| Sithandiwe E Mazibuko-Mbeje, Khanyisani Ziqubu, Phiwayinkosi V Dludla, Luca Tiano, Sonia Silvestri, Patrick Orlando, Thembeka A Nyawo, Johan Louw, Abidemi P Kappo, Christo J F Mulle. Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes. Metabolism open. vol 6. 2020-09-28. PMID:32812911. |
relevant bioassays and western blot analysis were conducted on these insulin resistant cells. |
2020-09-28 |
2023-08-13 |
Not clear |
| Yan Qin Tan, Qing Li, Lin Wang, Leo C Chiu-Leung, Lai K Leun. The livestock growth-promoter zeranol facilitates GLUT4 translocation in 3T3 L1 adipocytes. Chemosphere. vol 253. 2020-07-16. PMID:32464760. |
the insulin signal transduction pathway of glucose translocation was analyzed by western blot analysis. |
2020-07-16 |
2023-08-13 |
Not clear |
| Yan Gao, Juntong Li, Shifeng Chu, Zhao Zhang, Naihong Chen, Lin Li, Lan Zhan. Ginsenoside Rg1 protects mice against streptozotocin-induced type 1 diabetic by modulating the NLRP3 and Keap1/Nrf2/HO-1 pathways. European journal of pharmacology. vol 866. 2020-06-02. PMID:31738935. |
using elisa and western blot analysis, we found that rg1 attenuated abnormally elevated blood glucose, reduced inflammatory factors il-1β and il-18 in the blood, decreased alt and ast levels, promoted insulin secretion, and weakened the function of nlrp3 in mouse liver and pancreas. |
2020-06-02 |
2023-08-13 |
mouse |
| Kaiwu He, Lulin Nie, Qiang Zhou, Shafiq Ur Rahman, Jianjun Liu, Xifei Yang, Shupeng L. Proteomic Profiles of the Early Mitochondrial Changes in APP/PS1 and ApoE4 Transgenic Mice Models of Alzheimer's Disease. Journal of proteome research. vol 18. issue 6. 2020-05-27. PMID:31055927. |
the abnormal changes of insulin signaling and mitochondrial electron transport chain were further verified by western blot. |
2020-05-27 |
2023-08-13 |
mouse |
| Marcia Hiriart-Urdanivia, Carmen Sánchez-Soto, Myrian Velasco, Jean Sabido-Barrera, Rosa Isela Ortiz-Huidobr. Soluble insulin receptor and metabolic syndrome. Gaceta medica de Mexico. vol 155. issue 5. 2020-05-22. PMID:32091018. |
electrophoresis of the rats' plasma proteins was performed, followed by western blot (wb) for insulin and for the outer portion of the insulin receptor. |
2020-05-22 |
2023-08-13 |
rat |
| Heidy Cabrera-Cruz, Lorena Oróstica, Francisca Plaza-Parrochia, Ignacio Torres-Pinto, Carmen Romero, Margarita Veg. The insulin-sensitizing mechanism of myo-inositol is associated with AMPK activation and GLUT-4 expression in human endometrial cells exposed to a PCOS environment. American journal of physiology. Endocrinology and metabolism. vol 318. issue 2. 2020-05-04. PMID:31874063. |
smit-1 was assessed in endometrial tissue from women with normal weight, obesity, insulin resistance, and pcos; additionally, using an in vitro model of human endometrial cells exposed to an environment resembling hyperinsulinemic-obese-pcos, myo effect was evaluated on p-ampk and glut-4 levels and glucose uptake by western blot, immunocytochemistry, and confocal microscopy, respectively. |
2020-05-04 |
2023-08-13 |
human |
| Yan Hao, Weiwei Wang, Dong Wu, Kai Liu, Yihan Su. Bilobalide alleviates tumor necrosis factor-alpha-induced pancreatic beta-cell MIN6 apoptosis and dysfunction through upregulation of miR-153. Phytotherapy research : PTR. vol 34. issue 2. 2020-04-30. PMID:31667906. |
min6 cells were treated by tnf-α; then, viability, apoptosis, and insulin secretion were assessed by performing cell counting kit-8 assay, flow cytometry, glucose-stimulated insulin secretion assay, and western blot. |
2020-04-30 |
2023-08-13 |
Not clear |
| Xu-Wen Tang, Qing-Xin Qi. miR-335-5p induces insulin resistance and pancreatic islet β-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-β signaling pathway. Journal of cellular physiology. vol 234. issue 5. 2020-04-08. PMID:30341900. |
expressions of mir-335-5p, vash1, tgf-β1, and c-myc in pancreatic islet β-cells were determined by rt-qpcr, western blot analysis, and insulin release by elisa. |
2020-04-08 |
2023-08-13 |
mouse |
| Marcia Hiriart-Urdanivia, Carmen Sánchez-Soto, Myrian Velasco, Jean Sabido-Barrera, Rosa Isela Ortiz-Huidobr. El receptor soluble de insulina y el síndrome metabólico. Gaceta medica de Mexico. vol 155. issue 5. 2020-03-30. PMID:31695236. |
electrophoresis of the rats' plasma proteins was performed, followed by western blot (wb) for insulin and for the outer portion of the insulin receptor. |
2020-03-30 |
2023-08-13 |
rat |
| Yibing Guo, Saisai Chen, Liancheng Xu, Yan Huang, Yang Xu, Yuhua Lu, Zhiwei Wan. Decellularized and solubilized pancreatic stroma promotes the in vitro proliferation, migration and differentiation of BMSCs into IPCs. Cell and tissue banking. vol 20. issue 3. 2020-02-24. PMID:31270642. |
the differences on the cytological behavior with or without stroma were evaluated by morphological observation, insulin release assay, qrt-pcr assay and western blot analysis. |
2020-02-24 |
2023-08-13 |
Not clear |