| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xin-Yu Huang, Liang Zhang, Jian Sun, Neng-Gui Xu, Wei Y. Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats. Evidence-based complementary and alternative medicine : eCAM. vol 2016. 2020-09-30. PMID:27738449. |
protein and mrna expressions of insulin signaling molecules were determined by western blot and real-time rt-pcr, respectively. |
2020-09-30 |
2023-08-13 |
rat |
| Xiao Wei, Dan Zhu, Chenchen Feng, Guofang Chen, Xiaodong Mao, Qifeng Wang, Jie Wang, Chao Li. Inhibition of peptidyl-prolyl cis-trans isomerase B mediates cyclosporin A-induced apoptosis of islet β cells. Experimental and therapeutic medicine. vol 16. issue 5. 2020-09-29. PMID:30344674. |
expression levels of insulin, peptidyl-prolyl cis-trans isomerase b (ppib), cleaved caspase-3, phospho-protein kinase r (pkr)-like endoplasmic reticulum kinase (p-perk), pkr-like endoplasmic reticulum kinase (perk), binding immunoglobulin protein (bip), and c/ebp homologous protein (chop) were detected via reducing western blot assay. |
2020-09-29 |
2023-08-13 |
Not clear |
| Changjun Yun, Wenjie Qian, Junyi Wu, Chenxi Yuan, Songzhou Jiang, Jinpeng L. Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway. Experimental and therapeutic medicine. vol 19. issue 2. 2020-09-28. PMID:32010253. |
to determine whether pap can promote osteoblast development and to elucidate the molecular mechanisms underlying its functions, the present study investigated the effects of pap on osteoblast proliferation, differentiation and mineralization, and the role of the insulin signaling pathway using mtt assay, alkaline phosphatase activity assay, western blot analysis and reverse transcription-quantitative pcr. |
2020-09-28 |
2023-08-13 |
Not clear |
| Shivasadat Gheflat, Abdolrahim Sadeghi, Mojgan Bandehpour, Keyvan Ramezani, Bahram Kazem. Designing an Engineered Construct Gene Sensitive to Carbohydrate In-vitro and Candidate for Human Insulin Gene Therapy In-vivo. Iranian journal of pharmaceutical research : IJPR. vol 18. issue 4. 2020-09-28. PMID:32184874. |
insulin expression in the transfected cells with 200 and 400 ng of the construct in comparison with negative control was detected using western blot and elisa methods. |
2020-09-28 |
2023-08-13 |
human |
| Sithandiwe E Mazibuko-Mbeje, Khanyisani Ziqubu, Phiwayinkosi V Dludla, Luca Tiano, Sonia Silvestri, Patrick Orlando, Thembeka A Nyawo, Johan Louw, Abidemi P Kappo, Christo J F Mulle. Isoorientin ameliorates lipid accumulation by regulating fat browning in palmitate-exposed 3T3-L1 adipocytes. Metabolism open. vol 6. 2020-09-28. PMID:32812911. |
relevant bioassays and western blot analysis were conducted on these insulin resistant cells. |
2020-09-28 |
2023-08-13 |
Not clear |
| Yan Qin Tan, Qing Li, Lin Wang, Leo C Chiu-Leung, Lai K Leun. The livestock growth-promoter zeranol facilitates GLUT4 translocation in 3T3 L1 adipocytes. Chemosphere. vol 253. 2020-07-16. PMID:32464760. |
the insulin signal transduction pathway of glucose translocation was analyzed by western blot analysis. |
2020-07-16 |
2023-08-13 |
Not clear |
| Yan Gao, Juntong Li, Shifeng Chu, Zhao Zhang, Naihong Chen, Lin Li, Lan Zhan. Ginsenoside Rg1 protects mice against streptozotocin-induced type 1 diabetic by modulating the NLRP3 and Keap1/Nrf2/HO-1 pathways. European journal of pharmacology. vol 866. 2020-06-02. PMID:31738935. |
using elisa and western blot analysis, we found that rg1 attenuated abnormally elevated blood glucose, reduced inflammatory factors il-1β and il-18 in the blood, decreased alt and ast levels, promoted insulin secretion, and weakened the function of nlrp3 in mouse liver and pancreas. |
2020-06-02 |
2023-08-13 |
mouse |
| Kaiwu He, Lulin Nie, Qiang Zhou, Shafiq Ur Rahman, Jianjun Liu, Xifei Yang, Shupeng L. Proteomic Profiles of the Early Mitochondrial Changes in APP/PS1 and ApoE4 Transgenic Mice Models of Alzheimer's Disease. Journal of proteome research. vol 18. issue 6. 2020-05-27. PMID:31055927. |
the abnormal changes of insulin signaling and mitochondrial electron transport chain were further verified by western blot. |
2020-05-27 |
2023-08-13 |
mouse |
| Marcia Hiriart-Urdanivia, Carmen Sánchez-Soto, Myrian Velasco, Jean Sabido-Barrera, Rosa Isela Ortiz-Huidobr. Soluble insulin receptor and metabolic syndrome. Gaceta medica de Mexico. vol 155. issue 5. 2020-05-22. PMID:32091018. |
electrophoresis of the rats' plasma proteins was performed, followed by western blot (wb) for insulin and for the outer portion of the insulin receptor. |
2020-05-22 |
2023-08-13 |
rat |
| Heidy Cabrera-Cruz, Lorena Oróstica, Francisca Plaza-Parrochia, Ignacio Torres-Pinto, Carmen Romero, Margarita Veg. The insulin-sensitizing mechanism of myo-inositol is associated with AMPK activation and GLUT-4 expression in human endometrial cells exposed to a PCOS environment. American journal of physiology. Endocrinology and metabolism. vol 318. issue 2. 2020-05-04. PMID:31874063. |
smit-1 was assessed in endometrial tissue from women with normal weight, obesity, insulin resistance, and pcos; additionally, using an in vitro model of human endometrial cells exposed to an environment resembling hyperinsulinemic-obese-pcos, myo effect was evaluated on p-ampk and glut-4 levels and glucose uptake by western blot, immunocytochemistry, and confocal microscopy, respectively. |
2020-05-04 |
2023-08-13 |
human |
| Yan Hao, Weiwei Wang, Dong Wu, Kai Liu, Yihan Su. Bilobalide alleviates tumor necrosis factor-alpha-induced pancreatic beta-cell MIN6 apoptosis and dysfunction through upregulation of miR-153. Phytotherapy research : PTR. vol 34. issue 2. 2020-04-30. PMID:31667906. |
min6 cells were treated by tnf-α; then, viability, apoptosis, and insulin secretion were assessed by performing cell counting kit-8 assay, flow cytometry, glucose-stimulated insulin secretion assay, and western blot. |
2020-04-30 |
2023-08-13 |
Not clear |
| Xu-Wen Tang, Qing-Xin Qi. miR-335-5p induces insulin resistance and pancreatic islet β-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-β signaling pathway. Journal of cellular physiology. vol 234. issue 5. 2020-04-08. PMID:30341900. |
expressions of mir-335-5p, vash1, tgf-β1, and c-myc in pancreatic islet β-cells were determined by rt-qpcr, western blot analysis, and insulin release by elisa. |
2020-04-08 |
2023-08-13 |
mouse |
| Marcia Hiriart-Urdanivia, Carmen Sánchez-Soto, Myrian Velasco, Jean Sabido-Barrera, Rosa Isela Ortiz-Huidobr. El receptor soluble de insulina y el síndrome metabólico. Gaceta medica de Mexico. vol 155. issue 5. 2020-03-30. PMID:31695236. |
electrophoresis of the rats' plasma proteins was performed, followed by western blot (wb) for insulin and for the outer portion of the insulin receptor. |
2020-03-30 |
2023-08-13 |
rat |
| Yibing Guo, Saisai Chen, Liancheng Xu, Yan Huang, Yang Xu, Yuhua Lu, Zhiwei Wan. Decellularized and solubilized pancreatic stroma promotes the in vitro proliferation, migration and differentiation of BMSCs into IPCs. Cell and tissue banking. vol 20. issue 3. 2020-02-24. PMID:31270642. |
the differences on the cytological behavior with or without stroma were evaluated by morphological observation, insulin release assay, qrt-pcr assay and western blot analysis. |
2020-02-24 |
2023-08-13 |
Not clear |
| Seung-Il Choi, Boram Lee, Jong Hwan Woo, Jang Bin Jeong, Ikhyun Jun, Eung Kweon Ki. APP processing and metabolism in corneal fibroblasts and epithelium as a potential biomarker for Alzheimer's disease. Experimental eye research. vol 182. 2020-02-17. PMID:30930125. |
western blot analysis also demonstrated the expression of a disintegrin and metalloprotease domain-containing protein 10 (adam10), beta-site app-cleaving enzyme 1 (bace1), nicastrin, insulin degradation enzyme, and neprilysin in corneal fibroblasts. |
2020-02-17 |
2023-08-13 |
mouse |
| Xiao-Xuan Guo, Zhu Zeng, Yong-Zhong Qian, Jing Qiu, Kai Wang, Yong Wang, Bao-Ping Ji, Feng Zho. Wheat Flour, Enriched with γ-Oryzanol, Phytosterol, and Ferulic Acid, Alleviates Lipid and Glucose Metabolism in High-Fat-Fructose-Fed Rats. Nutrients. vol 11. issue 7. 2020-01-29. PMID:31340583. |
western blot analysis of proteins from liver samples reveals that these enriched flours alleviated hepatic lipid accumulation and insulin resistance through their elevation in the phosphorylation of ampk and akt. |
2020-01-29 |
2023-08-13 |
rat |
| Yingying Zhao, Chuanqian Liu, Xueting Zhang, Xipeng Ya. Angelica polysaccharide alleviates TNF-α-induced MIN6 cell damage a through the up-regulation microRNA-143. BioFactors (Oxford, England). 2020-01-08. PMID:31746030. |
the insulin, mir-143 and relative proteins and the inducible nitric oxide synthase (inos) were individually determined via gsis assay, qrt-pcr, and western blot. |
2020-01-08 |
2023-08-13 |
Not clear |
| Ana Babic Perhoc, Jelena Osmanovic Barilar, Ana Knezovic, Vladimir Farkas, Robert Bagaric, Alfred Svarc, Edna Grünblatt, Peter Riederer, Melita Salkovic-Petrisi. Cognitive, behavioral and metabolic effects of oral galactose treatment in the transgenic Tg2576 mice. Neuropharmacology. vol 148. 2019-12-27. PMID:30571958. |
the level of insulin, glucagon-like peptide-1 (glp-1) and soluble amyloid β1-42 (saβ1-42) was measured by elisa and the protein expression of insulin receptor (ir), glycogen synthase kinase-3β (gsk-3β), and pre-/post-synaptic markers by western blot analysis. |
2019-12-27 |
2023-08-13 |
mouse |
| Lin Yin, Ming-Xin Liu, Feng-Yuan Wang, Xi Wang, Yan-Hong Tang, Qing-Yan Zhao, Teng Wang, Yu-Ting Chen, Cong-Xin Huan. Transcription Factor prrx1 Promotes Brown Adipose-Derived Stem Cells Differentiation to Sinus Node-Like Cells. DNA and cell biology. vol 38. issue 11. 2019-12-03. PMID:31545082. |
after 5-7 days of adenoviral transfection, the expression levels of sinus node cell-associated pacing protein (hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 [hcn4]) and ion channel (calcium channel, voltage-dependent, t type, alpha 1g subunit [cacna1g]), as well as transcription factors (t-box 18 [tbx18], insulin gene enhancer binding protein 1 [isl-1], paired-like homeodomain transcription factor 2 [pitx2], short stature homeobox 2 [shox2]), were detected by western blot and reverse transcription-quantitative polymerase chain reaction. |
2019-12-03 |
2023-08-13 |
Not clear |
| Liang Yin, Qian Wang, Xiaohui Wang, Liang-Nian Son. Effects of Tribulus terrestris saponins on exercise performance in overtraining rats and the underlying mechanisms. Canadian journal of physiology and pharmacology. vol 94. issue 11. 2019-11-20. PMID:27482746. |
serum levels of testosterone and corticosterone and levels of androgen receptor (ar) and insulin growth factor-1 receptor (igf-1r) in the liver, gastrocnemius, and soleus were determined by elisa and western blot. |
2019-11-20 |
2023-08-13 |
rat |