| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Chan Yang, Hanyu Liu, Ziyan Xie, Qiangfei Yang, Lian Du, Chunguang Xi. The protective role of shenqi compound in type 2 diabetes: A comprehensive investigation of pancreatic β-cell function and mass. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 166. 2023-08-12. PMID:37572639. |
the results demonstrate multiple beneficial effects of sqc, including significant control of blood glucose levels (p < 0.05), inhibition of insulin resistance (measured by western blot), reduction of hyperinsulinemia (p < 0.05), attenuation of oxidative stress (p < 0.05), suppression of inflammation (p < 0.05), protection against islet hypertrophy and beta cell proliferation (evaluated through pathological staining), and inhibition of β-cell apoptosis and senescence (also assessed through pathological staining). |
2023-08-12 |
2023-08-16 |
rat |
| Minchuan Zhou, Jingqing Huang, Jingwen Zhou, Cuiting Zhi, Yan Bai, Qishi Che, Hua Cao, Jiao Guo, Zhengquan S. Anti-Obesity Effect and Mechanism of Chitooligosaccharides Were Revealed Based on Lipidomics in Diet-Induced Obese Mice. Molecules (Basel, Switzerland). vol 28. issue 14. 2023-07-29. PMID:37513467. |
the western blot assay demonstrated that cosm intervention can improve insulin resistance, inhibit de novo synthesis, and promote thermogenesis and β-oxidation in mitochondria by the ampk pathway, thereby alleviating high-fat diet-induced obesity. |
2023-07-29 |
2023-08-14 |
mouse |
| Ranran Sun, Liwei Jiang, Wenlong Chen, Yuanhao Xu, Xin Yi, Guohua Zhon. Azadirachtin exposure inhibit ovary development of Spodoptera litura (Lepidoptera: Noctuidae) by altering lipids metabolism event and inhibiting insulin signaling pathways. Ecotoxicology and environmental safety. vol 262. 2023-06-25. PMID:37356396. |
besides, following the abnormal lipids metabolism, western blot analysis suggested that aza induce insulin resistance-like phenotypes by inhibiting insulin receptor substrates (irs) /pi3k/akt pathway, which might be responsible for the ovary abnormalities of s. litura. |
2023-06-25 |
2023-08-14 |
Not clear |
| Jing Xu, Xiaoyu Fan, Mou Zhu, Zhenglin Zhao, Xiaodan Qi, Han Gao, Ying Liu, Yan Shi, Chunjing Zhan. Ginsenoside Rg3 Protects Mouse Islet β-Cells Injured by High Glucose. Indian journal of microbiology. vol 63. issue 2. 2023-06-16. PMID:37325021. |
the cultured mouse pancreatic islet min6 cells were divided into control group (nc), rg3 group (rg3, 50 μg/l), high glucose group (hg, 33 mmol/l), high glucose and rg3 group (hg + rg3), after 48 h of continuous culture, cck-8 was used to detect cell viability; mouse insulin enzyme-linked immunoassay kit to detect insulin release; atp content detection kit to detect atp; dcfh-da to detect intracellular reactive oxygen species (ros) levels; total glutathione (t-gsh)/oxidized glutathione (gssg) assay kit to detect the ratio of gsh/gssg; using the mitochondrial membrane channel pore (mptp) fluorescence detection kit in min6 cells and collect the intensity of green fluorescence; western blot to detect the expression of antioxidant proteins glutathione reductase (gr). |
2023-06-16 |
2023-08-14 |
mouse |
| Hakan Soylu, Kayihan Karaco. The effects of hydroxytyrosol on Prdx6 and insulin expression in diabetic rat pancreases. Histochemistry and cell biology. 2023-05-23. PMID:37219732. |
insulin expression was determined by immunohistochemistry and prdx6 expression was determined by immunohistochemistry and western blot. |
2023-05-23 |
2023-08-14 |
rat |
| Lihui Ni, Yuan Li, Haifeng Zhang, Yunxiao Ma, Yuli Song, Xudong Tang, Junwen Fan, Jin Yang Shi, Xinhua Cui, Hongyue Xu, Hong Zhou, Keshu Shen, Weiying Guo, Lu Y. The combination of insulin and linezolid ameliorates Staphylococcus aureus pneumonia in individuals with diabetes via the TLR2/MAPKs/NLRP3 pathway. International journal of biological macromolecules. 2023-05-09. PMID:37160172. |
in vitro, our drug sensitivity assay, bacterial growth curve and hemolytic tests showed that a combination of insulin and linezolid exerted good antibacterial and anti-α-hemolysin activity, cck8 experiment, glucose content and glycogen content determination showed that the combination of insulin and linezolid increased murine macrophage survival rate and reduced the extracellular glucose level of high glucose-treated mh-s cells and intracellular glycogen level, and western blot showed that the combination inhibited tlr2/mapks/nlrp3-related inflammatory pathways in mh-s cells. |
2023-05-09 |
2023-08-14 |
mouse |
| Ana Knezovic, Marija Piknjac, Jelena Osmanovic Barilar, Ana Babic Perhoc, Davor Virag, Jan Homolak, Melita Salkovic-Petrisi. Association of Cognitive Deficit with Glutamate and Insulin Signaling in a Rat Model of Parkinson's Disease. Biomedicines. vol 11. issue 3. 2023-03-29. PMID:36979662. |
the expression of tyrosine hydroxylase (th) and proteins involved in insulin (insulin receptor - ir, phosphoinositide 3 kinase - pi3k, extracellular signal-regulated kinases-erk) and glutamate receptor (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptos-ampar, n-methyl-d-aspartate receptor - nmdar) signaling was assessed in the hippocampus (hpc), hypothalamus (hpt) and striatum (s) by immunofluorescence, western blot and enzyme-linked immunosorbent assay (elisa). |
2023-03-29 |
2023-08-14 |
rat |
| Man Zhao, Qian Meng, Man Zhan. Urinary insulin signaling pathway related proteins may serve as potential biomarkers for monitoring diabetes mellitus without hypertension and hyperlipidemia. Medicine. vol 102. issue 5. 2023-02-07. PMID:36749274. |
western blot and enzyme-linked immunoassay were performed to verify the expression of insulin signaling pathway related proteins in the urine of diabetic patients. |
2023-02-07 |
2023-08-14 |
Not clear |
| Xueyao Yin, Zhiye Xu, Xinxin Zhang, Jiahua Wu, Weina L. Deficiency of lipopolysaccharide binding protein facilitates adipose browning, glucose uptake and oxygen consumption in mouse embryonic fibroblasts via activating PI3K/Akt/mTOR pathway and inhibiting autophagy. Cell cycle (Georgetown, Tex.). 2023-01-30. PMID:36710409. |
the expression levels of lbp, inflammatory markers , brown fat markers, lipid metabolism marker, autophagy markers, insulin signaling-related molecules , p-mtor, mtor, p-akt, akt, p-pi3k, and pi3k were quantified or determined by western blot, qrt-pcr, and immunofluorescence assay. |
2023-01-30 |
2023-08-14 |
mouse |
| Tingtian Li, Yafei Wang, Shujuan Huang, Huaping Tan. The Regulation Mechanism of MUC5AC Secretion in Airway of Obese Asthma. Cellular and molecular biology (Noisy-le-Grand, France). vol 68. issue 7. 2022-12-10. PMID:36495503. |
compared with the control group, 100 fxmol and l1 efaroxan promoted insulin secretion (p <0.01), and administration of the muc5ac antagonist ku14r significantly inhibited the effect of muc5ac.western blot showed that the protein expression levels of β-catenin, c-myc and cyclin d1 in asmc of the obese asthma group were significantly higher than those of the control group (p <0.05), while the protein expression level of gsk-3β was lower than control group (p <0.05). |
2022-12-10 |
2023-08-14 |
rat |
| Priyanka Rajan, Premkumar Natraj, Sachithra S Ranaweera, Lakshi A Dayarathne, Young Jae Lee, Chang-Hoon Ha. Anti-diabetic effect of hesperidin on palmitate (PA)-treated HepG2 cells and high fat diet-induced obese mice. Food research international (Ottawa, Ont.). vol 162. issue Pt B. 2022-12-03. PMID:36461387. |
furthermore, western blot analysis confirmed that hes improved insulin sensitivity and glucose homeostasis by normalizing the expression levels of hexokinase-ii, enolase-1, and pi3 kinase p110δ to normal level. |
2022-12-03 |
2023-08-14 |
mouse |
| Heloisa B Assalin, Kelly Cristiane Gabriel De Almeida, Dioze Guadagnini, Andrey Santos, Caio J Teixeira, Silvana Bordin, Guilherme Z Rocha, Mario J A Saa. Proton Pump Inhibitor Pantoprazole Modulates Intestinal Microbiota and Induces TLR4 Signaling and Fibrosis in Mouse Liver. International journal of molecular sciences. vol 23. issue 22. 2022-11-26. PMID:36430244. |
we performed glucose (gtt) and insulin (itt) tolerance tests, serum lipopolysaccharide (lps) dosage, liver histology, liver and intestine extraction for western blot and qpcr. |
2022-11-26 |
2023-08-14 |
mouse |
| Wan-Hui Guo, Qi Guo, Ya-Lin Liu, Dan-Dan Yan, Li Jin, Rong Zhang, Jing Yan, Xiang-Hang Luo, Mi Yan. Mutated lncRNA increase the risk of type 2 diabetes by promoting β cell dysfunction and insulin resistance. Cell death & disease. vol 13. issue 10. 2022-10-27. PMID:36302749. |
mass spectrometry assay of proteins after rna pulldown demonstrate that mut-reg1cp directly binds to polypyrimidine tract binding protein 1 (ptbp1), further immunofluorescence staining, western blot analysis, qpcr analysis and glucose stimulated insulin secretion test reveal that mut-reg1cp disrupts the stabilization of insulin mrna by inhibiting the phosphorylation of ptbp1 in β cells. |
2022-10-27 |
2023-08-14 |
mouse |
| Xin Xiao, Wen-Hua Xu, Xiao-Qing Zhang, Jun-Feng Ding, Yue Jiang, Jun T. [Anti-oxidative and anti-apoptotic effects and molecular mechanisms of catalpol against H_2O_2-induced oxidative damage in pancreatic β cells (INS-1 cells)]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 16. 2022-09-01. PMID:36046869. |
the present study investigated the anti-oxidative and anti-apoptotic effects and molecular mechanisms of catalpol on the h_2o_2-induced pancreatic β-cells(ins-1 cells).the oxidative damage model of ins-1 cells was induced and optimized by the stimulation of h_2o_2 of different concentrations for different time.cck-8 assay was used to detect cell viability after catalpol intervention(1, 5, 10, 20, 40, 80, and 160 μmol·l~(-1)) for 24 h.intracellular reactive oxygen species(ros), superoxide dismutase(sod), and lipid peroxide malondialdehyde(mda) were measured by dcfh-da fluorescent probe, wst-1, and tba respectively.moreover, the apo-ptotic effect was detected by ao-eb and annexin v-fitc/pi staining.in addition, the protein expression levels were detected by wes-tern blot, and intracellular insulin concentration was measured by elisa.the results showed that the oxidative damage model of ins-1 cells was stably induced by 50 μmol·l~(-1) h_2o_2 treatment for 2 h, and catalpol at 1-80 μmol·l~(-1) did not affect cell viability of ins-1 cells.compared with the conditions in the model group, 1, 5, and 10 μmol·l~(-1) catalpol intervention for 2 h could protect ins-1 cells from oxidative damage(p<0.001), reduce ros and mda, increase sod, and inhibit excessive cell apoptosis.moreover, 1, 5, and 10 μmol·l~(-1) catalpol could also up-regulate the phosphorylation of nuclear transcription factor nf-e2 related factors, negatively regulate kelch-like ech-associated protein 1(keap1), phosphorylation of extracellular signal-regulated kinase(erk), and heme oxyge-nase 1(ho-1), and promote the protein expression of pancreatic-duodenal homeobox factor-1(pdx-1) and glucose transporter 2(glut2).in addition, 1, 5, and 10 μmol·l~(-1) catalpol increased insulin secretion of ins-1 cells under oxidative damage in the high-glucose culture medium, indicating function recovery of pancreatic β cells.pdx-1 is a key nuclear transcription factor of pancreatic β cell function that directly regulates glut2 and insulin synthesis, and affects glucose homeostasis.in conclusion, catalpol can reduce the oxidative damage and apoptosis of ins-1 cells, activate antioxidant pathway, protect the function of pancreatic β cells, and improve insulin synthesis and secretion. |
2022-09-01 |
2023-08-14 |
Not clear |
| Ya-Fen Pang, Ming Huang, Lin Li, Xin Zhao, Hong-Xia Zhang, Yu-Hong L. [Effect of Danlou Tablets on alleviating hepatic adipogenesis, inflammatory response, and insulin resistance in db/db mice]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 12. 2022-07-19. PMID:35851126. |
western blot was performed to determine the protein levels of insulin receptor-β(irβ), phospho-irβ(p-irβ), phosphatidylinositol 3-kinase(pi3 k), and insulin receptor substrate-1(irs-1) involved in irs-1/pi3 k/akt signaling pathway in the livers of mice to reveal the mechanism of dlt in alleviating insulin resistance in diabetic mice. |
2022-07-19 |
2023-08-14 |
mouse |
| Ruili Yin, Yan Ma, Ning Zhang, Longyan Yang, Dong Zha. Combined effects of voluntary running and liraglutide on glucose homeostasis, fatty acid composition of brown adipose tissue phospholipids, and white adipose tissue browning in db/db mice. The Chinese journal of physiology. vol 65. issue 3. 2022-07-01. PMID:35775530. |
fasting blood glucose was monitored every week, plasma insulin and lipid profiles were analyzed, and thermogenic protein expression in bat and white adipose tissue (wat) were analyzed by the western blot. |
2022-07-01 |
2023-08-14 |
mouse |
| Xiangjuan Wei, Daqian Yang, Boya Zhang, Xingpei Fan, Haining Du, Ruijiao Zhu, Xiaotong Sun, Meimei Zhao, Ning G. Di-(2-ethylhexyl) phthalate increases plasma glucose and induces lipid metabolic disorders via FoxO1 in adult mice. The Science of the total environment. 2022-06-24. PMID:35750186. |
western blot and real-time quantitative pcr showed that dehp induced insulin resistance and promoted gluconeogenesis and lipid accumulation via overexpression of forkhead box protein o1 (foxo1), in keeping with hepatic rna sequencing data. |
2022-06-24 |
2023-08-14 |
mouse |
| Lushan Wang, Timothy S Wiedmann, Karunya K Kandimall. Modulating Insulin Signaling and Trafficking at the Blood-Brain Barrier Endothelium Using Lipid Based Nanoemulsions. International journal of pharmaceutics. 2022-05-23. PMID:35605891. |
moreover, western blot and flow cytometry analysis showed that snes containing polyunsaturated fatty acids (pufas) increased phospo-akt (p-akt) expression, a marker for the stimulation of metabolic arm of the insulin signaling, and insulin uptake in human cerebral microvascular endothelial cell (hcmec/d3) monolayers. |
2022-05-23 |
2023-08-13 |
human |
| Lian-Zhi Cheng, Jia-Mei Zhou, Jun-Long Ma, Fan-Jing Wang, Kai Cheng, Qian Chen, Hui-Lun Yuan, Ai-Juan Jian. [Tetrahydropalmatine alleviated diabetic neuropathic pain by inhibiting activation of microglia via p38 MAPK signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 9. 2022-05-09. PMID:35531701. |
the protein expression levels of ox42, inducible nitric oxide synthase(inos), cd206, p38, and p-p38 were determined by western blot, the fluorescence expression levels of ox42 and p-p38 in the dorsal horn of the rat spinal cord by immunofluorescence, the mrna content of p38 and ox42 in rat spinal cord tissue by qrt-pcr, and levels of nitric oxide(no), interleukin-1β(il-1β), interleukin-6(il-6), tumor necrosis factor-α(tnf-α), interleukin-10(il-10), and serum fasting insulin(fins) by enzyme-linked immunosorbent assay(elisa). |
2022-05-09 |
2023-08-13 |
rat |
| Maryam Bazrgar, Pariya Khodabakhsh, Leila Dargahi, Fatemeh Mohagheghi, Abolhassan Ahmadian. MicroRNA modulation is a potential molecular mechanism for neuroprotective effects of intranasal insulin administration in amyloid βeta oligomer induced Alzheimer's like rat model. Experimental gerontology. 2022-04-27. PMID:35476966. |
then, molecular and histological changes in response to aβo and/or insulin time course were assessed in the extracted hippocampi on days 1, 14, and 21 of the study using congo red staining, western blot and quantitative real-time pcr analyses. |
2022-04-27 |
2023-08-13 |
rat |