| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Guoqing Zhang, Ran Li, Wu Li, Sijia Yang, Qing Sun, Hongping Yin, Cui Wang, Biyu Hou, Huanhuan Wang, Liping Yu, Rucheng Chen, Liyun Shi, Kezhong Zhang, Chong Wee Liew, Guifen Qiang, Qinghua Sun, Cuiqing Li. Toll-like receptor 3 ablation prevented high-fat diet-induced obesity and metabolic disorder. The Journal of nutritional biochemistry. vol 95. 2021-09-16. PMID:33965533. |
standard western blot, immunohistochemistry and quantative pcr were used to assess molecules in pathways about lipid and glucose metabolism, insulin and inflammation in tissues of liver and vat. |
2021-09-16 |
2023-08-13 |
mouse |
| Hui Yang, Lin Tang, Zhan Qu, Shi-Hui Lei, Wei Li, Yu-Hong Wan. Hippocampal insulin resistance and the Sirtuin 1 signaling pathway in diabetes-induced cognitive dysfunction. Neural regeneration research. vol 16. issue 12. 2021-08-26. PMID:33907035. |
at 8 weeks after streptozotocin injection, the morris water maze test and western blot assays confirmed that the diabetic model rats had learning and memory deficits, insulin resistance, and sirtuin 1 expression could be detected in the hippocampus. |
2021-08-26 |
2023-08-13 |
rat |
| Steffen M Heckl, Franziska Mau, Anke Senftleben, Tina Daunke, Silje Beckinger, Samir Abdullazade, Stefan Schreiber, Christoph Röcken, Susanne Sebens, Heiner Schäfe. Programmed Death-Ligand 1 (PD-L1) Expression Is Induced by Insulin in Pancreatic Ductal Adenocarcinoma Cells Pointing to Its Role in Immune Checkpoint Control. Medical sciences (Basel, Switzerland). vol 9. issue 3. 2021-08-26. PMID:34202040. |
treatment of the pdac cell lines bxpc3, a818-6, and t3m4 with insulin increased pd-l1 expression in a time- and dose dependent fashion, as shown by western blot and qpcr analysis. |
2021-08-26 |
2023-08-13 |
human |
| Shuai-Nan Liu, Quan Liu, Lei Lei, Su-Juan Sun, Cai-Na Li, Yi Huan, Shao-Cong Hou, Zhu-Fang She. The Chinese patent medicine, Jin-tang-ning, ameliorates hyperglycemia through improving β cell function in pre-diabetic KKAy mice. Chinese journal of natural medicines. vol 18. issue 11. 2021-08-05. PMID:33308603. |
pancreatic protein expression levels of key factors involving in insulin secretion-related pathway and er stress were also detected by western blot. |
2021-08-05 |
2023-08-13 |
mouse |
| André Guaraci DeVito-Moraes, Victor Di Donato Marques, Luciana Chagas Caperuto, Flavia Kazue Ibuki, Fernando Neves Nogueira, Carlos Eduardo Francci, Carla Roberta de Oliveira Carvalh. INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats. Cell biochemistry and biophysics. 2021-08-04. PMID:34345983. |
herein we evaluated the acute effects of insulin on insulin receptor phosphorylation status and its substrates irs-1 and irs-2 in the parotid glands of adult male wistar rats, using western blot analyses. |
2021-08-04 |
2023-08-13 |
rat |
| Jessica I Selig, Joana Boulgaropoulos, Naima Niazy, D Margriet Ouwens, Karlheinz Preuß, Patrick Horn, Ralf Westenfeld, Artur Lichtenberg, Payam Akhyari, Mareike Bart. Crosstalk of Diabetic Conditions with Static Versus Dynamic Flow Environment-Impact on Aortic Valve Remodeling. International journal of molecular sciences. vol 22. issue 13. 2021-08-02. PMID:34203572. |
western blot analysis showed increased phosphorylation level of protein kinase b (akt) after acute insulin stimulation, which was lost in av under hyperinsulinemia, indicating acquired insulin resistance of the av tissue in response to elevated insulin levels. |
2021-08-02 |
2023-08-13 |
Not clear |
| Komal Rawal, Kishan M Purohit, Tushar P Patel, Neeta Karont, Sarita Gupt. Resistin mitigates stemness and metabolic profile of human adipose-derived mesenchymal stem cells via insulin resistance. Cytokine. vol 138. 2021-07-26. PMID:33271386. |
insulin sensitivity was examined by western blot and glucose uptake assay. |
2021-07-26 |
2023-08-13 |
human |
| Ricardo Augusto Leoni De Sousa, Alex Cleber Improta-Caria, Francine Menezes de Jesus-Silva, Caique Olegário Diniz E Magalhães, Daniel Almeida Freitas, Ana Cristina Rodrigues Lacerda, Vanessa Amaral Mendonça, Ricardo Cardoso Cassilhas, Hércules Ribeiro Leit. High-intensity resistance training induces changes in cognitive function, but not in locomotor activity or anxious behavior in rats induced to type 2 diabetes. Physiology & behavior. vol 223. 2021-06-21. PMID:32505787. |
insulin resistance and neuronal death were evaluated through western blot analysis. |
2021-06-21 |
2023-08-13 |
rat |
| K P Briski, Md Haider Ali, Prabhat R Napi. Sex-specific acclimation of A2 noradrenergic neuron dopamine-β-hydroxylase and estrogen receptor variant protein and 5'-AMP-Activated protein kinase reactivity to recurring hypoglycemia in rat. Journal of chemical neuroanatomy. vol 109. 2021-06-14. PMID:32599255. |
a2 neurons were laser-catapult-microdissected from male and female rats after one or four insulin injections for western blot analysis. |
2021-06-14 |
2023-08-13 |
rat |
| Alexandre M Lehnen, Graziela H Pinto, Júlia Borges, Melissa M Markoski, Beatriz D Schaa. Adaptations in GLUT4 Expression in Response to Exercise Detraining Linked to Downregulation of Insulin-Dependent Pathways in Cardiac but not in Skeletal Muscle Tissue. International journal of sport nutrition and exercise metabolism. vol 30. issue 4. 2021-06-10. PMID:32454459. |
the authors evaluated insulin resistance, maximum effort capacity, glut4 content, p-irs-1tyr1179, p-as160ser588, p-ampkα1thr172, and p-camkiithr286 in cardiac and gastrocnemius muscle tissues (western blot). |
2021-06-10 |
2023-08-13 |
rat |
| Kristina Wardelmann, Michaela Rath, José Pedro Castro, Sabine Blümel, Mareike Schell, Robert Hauffe, Fabian Schumacher, Tanina Flore, Katrin Ritter, Andreas Wernitz, Toru Hosoi, Koichiro Ozawa, Burkhard Kleuser, Jürgen Weiß, Annette Schürmann, André Kleinridder. Central Acting Hsp10 Regulates Mitochondrial Function, Fatty Acid Metabolism, and Insulin Sensitivity in the Hypothalamus. Antioxidants (Basel, Switzerland). vol 10. issue 5. 2021-05-27. PMID:33946318. |
we analyzed mitochondrial function and insulin signaling utilizing qpcr, western blot, xf96 analyzer, immunohistochemistry, and microscopy techniques. |
2021-05-27 |
2023-08-13 |
mouse |
| Eugenio Cersosimo, Xiaojing Xu, Tomoko Terasawa, Lily Q Don. Anti-inflammatory and anti-proliferative action of adiponectin mediated by insulin signaling cascade in human vascular smooth muscle cells. Molecular biology reports. vol 47. issue 9. 2021-05-25. PMID:32789574. |
in primary cultures of vsmc exposed to high glucose and palmitate plus insulin, the expression of pi-3 kinase (akt and m-tor), map-kinase (erk and p-38) molecules, and inflammatory markers (tlr-4 and ikb-α) were assessed with western blot, in the absence/presence of adiporon (ar). |
2021-05-25 |
2023-08-13 |
human |
| Lin Zhao, Yang Liu, Xiangrong Wan. TNF-α promotes insulin resistance in obstructive sleep apnea-hypopnea syndrome. Experimental and therapeutic medicine. vol 21. issue 6. 2021-04-16. PMID:33850540. |
the expression of insulin resistance-associated factors, insulin receptor substrate 1 (irs1) and glut4, was determined by western blot analysis and immunohistochemistry. |
2021-04-16 |
2023-08-13 |
Not clear |
| Leela Paladugu, Abeer Gharaibeh, Nivya Kolli, Cameron Learman, Tia C Hall, Lixin Li, Julien Rossignol, Panchanan Maiti, Gary L Dunba. Liraglutide Has Anti-Inflammatory and Anti-Amyloid Properties in Streptozotocin-Induced and 5xFAD Mouse Models of Alzheimer's Disease. International journal of molecular sciences. vol 22. issue 2. 2021-04-07. PMID:33467075. |
the extent of neurodegeneration, aβ plaque load, and key proteins associated with the insulin signaling pathways were measured using western blot and neuroinflammation (via immunohistological assays) in the cortical and hippocampal regions of the brain were assessed following a series of behavioral tests used to measure cognitive function after lir or vehicle treatments. |
2021-04-07 |
2023-08-13 |
mouse |
| Ayse Karatug Kaca. Indomethacin decreases insulin secretion by reducing KCa3.1 as a biomarker of pancreatic tumor and causes apoptotic cell death. Journal of biochemical and molecular toxicology. vol 34. issue 7. 2021-02-23. PMID:32128977. |
the relationship between cell death and insulin metabolism was determined with the administration of indomethacin. the cell viability by wst-1; the apoptosis and necrosis levels by elisa kits; malondialdehyde levels by spectrophotometer; and beclin, intracellular insulin, insulin secretion, kca3.1, insulin receptor (ir), glucose transporter type 2 (glut2), activating transcription factor 2 (atf2), elk1, c-jun, akt and phosphorylated atf2, elk1, c-jun, akt, intracellular betacellulin and betacellulin secretion levels by western blot analysis investigated. the ins1, ins2, ir, glut2, atf2, elk1, c-jun, akt, and betacellulin gene expression levels were determined by the real-time quantitative reverse transcription-polymerase chain reaction method. |
2021-02-23 |
2023-08-13 |
rat |
| Jiayuan Chen, Li Gao, Yao Zhang, Ya Su, Zheng Kong, Dongqing Wang, Ming Ya. Acteoside-improved streptozotocin-induced learning and memory impairment by upregulating hippocampal insulin, glucose transport, and energy metabolism. Phytotherapy research : PTR. vol 35. issue 1. 2021-02-23. PMID:33029835. |
the acteoside induced increase in the protein expression of glucose transporters (glu t1, glu t3, and glu t4), glucose, and insulin levels in the hippocampus for maintaining normal learning and memory function were demonstrated by western blot. |
2021-02-23 |
2023-08-13 |
Not clear |
| Xiaoqing Yi, Xuan Cai, Sisi Wang, Yanfeng Xia. Mechanisms of impaired pancreatic β‑cell function in high‑fat diet‑induced obese mice: The role of endoplasmic reticulum stress. Molecular medicine reports. vol 21. issue 5. 2021-02-16. PMID:32323766. |
in vitro, ers was induced by palmitate (pa) in ins‑1 cells, and the protein expression of atf6, and mrna expression of atf6 and insulin were examined via western blot and quantitative pcr (qpcr) analyses, respectively. |
2021-02-16 |
2023-08-13 |
mouse |
| Jaison Jeevanandam, Yen San Chan, Michael K Danquah, Ming Chiat La. Cytotoxicity Analysis of Morphologically Different Sol-Gel-Synthesized MgO Nanoparticles and Their In Vitro Insulin Resistance Reversal Ability in Adipose cells. Applied biochemistry and biotechnology. vol 190. issue 4. 2021-01-29. PMID:31776944. |
dns glucose assay and western blot showed that hexagonally shaped mgo nanoparticles had reversed 29.5% of insulin resistance whilst fluorescence microscopy studies indicated that the insulin resistance reversal is due to the activation of intracellular enzymes. |
2021-01-29 |
2023-08-13 |
Not clear |
| Franziska Kopietz, Kaja Rupar, Christine Berggreen, Johanna Säll, Didier Vertommen, Eva Degerman, Mark H Rider, Olga Göransso. Inhibition of AMPK activity in response to insulin in adipocytes: involvement of AMPK pS485, PDEs, and cellular energy levels. American journal of physiology. Endocrinology and metabolism. vol 319. issue 3. 2020-12-30. PMID:32663099. |
ampk activity measurements by western blot analysis and in vitro kinase assay revealed that wt and s485a ampk were inhibited to a similar degree by insulin, indicating that ampk s485 phosphorylation is not required for insulin-induced ampk inhibition. |
2020-12-30 |
2023-08-13 |
human |
| Li Xiong, Yingying Gong, Liting Wu, Jin Li, Weiman He, Xiaonan Zhu, Haipeng Xia. LncRNA-Malat1 is Involved in Lipotoxicity-Induced ß-cell Dysfunction and the Therapeutic Effect of Exendin-4 via Ptbp1. Endocrinology. vol 161. issue 7. 2020-12-14. PMID:32324218. |
the results of tunel, western blot and flow cytometric analyses, and gsis assays revealed that malat1 knockdown significantly induced β-cell apoptosis and inhibited insulin secretion. |
2020-12-14 |
2023-08-13 |
mouse |