| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| André Guaraci DeVito-Moraes, Victor Di Donato Marques, Luciana Chagas Caperuto, Flavia Kazue Ibuki, Fernando Neves Nogueira, Carlos Eduardo Francci, Carla Roberta de Oliveira Carvalh. INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats. Cell biochemistry and biophysics. 2021-08-04. PMID:34345983. |
herein we evaluated the acute effects of insulin on insulin receptor phosphorylation status and its substrates irs-1 and irs-2 in the parotid glands of adult male wistar rats, using western blot analyses. |
2021-08-04 |
2023-08-13 |
rat |
| Jessica I Selig, Joana Boulgaropoulos, Naima Niazy, D Margriet Ouwens, Karlheinz Preuß, Patrick Horn, Ralf Westenfeld, Artur Lichtenberg, Payam Akhyari, Mareike Bart. Crosstalk of Diabetic Conditions with Static Versus Dynamic Flow Environment-Impact on Aortic Valve Remodeling. International journal of molecular sciences. vol 22. issue 13. 2021-08-02. PMID:34203572. |
western blot analysis showed increased phosphorylation level of protein kinase b (akt) after acute insulin stimulation, which was lost in av under hyperinsulinemia, indicating acquired insulin resistance of the av tissue in response to elevated insulin levels. |
2021-08-02 |
2023-08-13 |
Not clear |
| Komal Rawal, Kishan M Purohit, Tushar P Patel, Neeta Karont, Sarita Gupt. Resistin mitigates stemness and metabolic profile of human adipose-derived mesenchymal stem cells via insulin resistance. Cytokine. vol 138. 2021-07-26. PMID:33271386. |
insulin sensitivity was examined by western blot and glucose uptake assay. |
2021-07-26 |
2023-08-13 |
human |
| Ricardo Augusto Leoni De Sousa, Alex Cleber Improta-Caria, Francine Menezes de Jesus-Silva, Caique Olegário Diniz E Magalhães, Daniel Almeida Freitas, Ana Cristina Rodrigues Lacerda, Vanessa Amaral Mendonça, Ricardo Cardoso Cassilhas, Hércules Ribeiro Leit. High-intensity resistance training induces changes in cognitive function, but not in locomotor activity or anxious behavior in rats induced to type 2 diabetes. Physiology & behavior. vol 223. 2021-06-21. PMID:32505787. |
insulin resistance and neuronal death were evaluated through western blot analysis. |
2021-06-21 |
2023-08-13 |
rat |
| K P Briski, Md Haider Ali, Prabhat R Napi. Sex-specific acclimation of A2 noradrenergic neuron dopamine-β-hydroxylase and estrogen receptor variant protein and 5'-AMP-Activated protein kinase reactivity to recurring hypoglycemia in rat. Journal of chemical neuroanatomy. vol 109. 2021-06-14. PMID:32599255. |
a2 neurons were laser-catapult-microdissected from male and female rats after one or four insulin injections for western blot analysis. |
2021-06-14 |
2023-08-13 |
rat |
| Alexandre M Lehnen, Graziela H Pinto, Júlia Borges, Melissa M Markoski, Beatriz D Schaa. Adaptations in GLUT4 Expression in Response to Exercise Detraining Linked to Downregulation of Insulin-Dependent Pathways in Cardiac but not in Skeletal Muscle Tissue. International journal of sport nutrition and exercise metabolism. vol 30. issue 4. 2021-06-10. PMID:32454459. |
the authors evaluated insulin resistance, maximum effort capacity, glut4 content, p-irs-1tyr1179, p-as160ser588, p-ampkα1thr172, and p-camkiithr286 in cardiac and gastrocnemius muscle tissues (western blot). |
2021-06-10 |
2023-08-13 |
rat |
| Kristina Wardelmann, Michaela Rath, José Pedro Castro, Sabine Blümel, Mareike Schell, Robert Hauffe, Fabian Schumacher, Tanina Flore, Katrin Ritter, Andreas Wernitz, Toru Hosoi, Koichiro Ozawa, Burkhard Kleuser, Jürgen Weiß, Annette Schürmann, André Kleinridder. Central Acting Hsp10 Regulates Mitochondrial Function, Fatty Acid Metabolism, and Insulin Sensitivity in the Hypothalamus. Antioxidants (Basel, Switzerland). vol 10. issue 5. 2021-05-27. PMID:33946318. |
we analyzed mitochondrial function and insulin signaling utilizing qpcr, western blot, xf96 analyzer, immunohistochemistry, and microscopy techniques. |
2021-05-27 |
2023-08-13 |
mouse |
| Eugenio Cersosimo, Xiaojing Xu, Tomoko Terasawa, Lily Q Don. Anti-inflammatory and anti-proliferative action of adiponectin mediated by insulin signaling cascade in human vascular smooth muscle cells. Molecular biology reports. vol 47. issue 9. 2021-05-25. PMID:32789574. |
in primary cultures of vsmc exposed to high glucose and palmitate plus insulin, the expression of pi-3 kinase (akt and m-tor), map-kinase (erk and p-38) molecules, and inflammatory markers (tlr-4 and ikb-α) were assessed with western blot, in the absence/presence of adiporon (ar). |
2021-05-25 |
2023-08-13 |
human |
| Lin Zhao, Yang Liu, Xiangrong Wan. TNF-α promotes insulin resistance in obstructive sleep apnea-hypopnea syndrome. Experimental and therapeutic medicine. vol 21. issue 6. 2021-04-16. PMID:33850540. |
the expression of insulin resistance-associated factors, insulin receptor substrate 1 (irs1) and glut4, was determined by western blot analysis and immunohistochemistry. |
2021-04-16 |
2023-08-13 |
Not clear |
| Leela Paladugu, Abeer Gharaibeh, Nivya Kolli, Cameron Learman, Tia C Hall, Lixin Li, Julien Rossignol, Panchanan Maiti, Gary L Dunba. Liraglutide Has Anti-Inflammatory and Anti-Amyloid Properties in Streptozotocin-Induced and 5xFAD Mouse Models of Alzheimer's Disease. International journal of molecular sciences. vol 22. issue 2. 2021-04-07. PMID:33467075. |
the extent of neurodegeneration, aβ plaque load, and key proteins associated with the insulin signaling pathways were measured using western blot and neuroinflammation (via immunohistological assays) in the cortical and hippocampal regions of the brain were assessed following a series of behavioral tests used to measure cognitive function after lir or vehicle treatments. |
2021-04-07 |
2023-08-13 |
mouse |
| Ayse Karatug Kaca. Indomethacin decreases insulin secretion by reducing KCa3.1 as a biomarker of pancreatic tumor and causes apoptotic cell death. Journal of biochemical and molecular toxicology. vol 34. issue 7. 2021-02-23. PMID:32128977. |
the relationship between cell death and insulin metabolism was determined with the administration of indomethacin. the cell viability by wst-1; the apoptosis and necrosis levels by elisa kits; malondialdehyde levels by spectrophotometer; and beclin, intracellular insulin, insulin secretion, kca3.1, insulin receptor (ir), glucose transporter type 2 (glut2), activating transcription factor 2 (atf2), elk1, c-jun, akt and phosphorylated atf2, elk1, c-jun, akt, intracellular betacellulin and betacellulin secretion levels by western blot analysis investigated. the ins1, ins2, ir, glut2, atf2, elk1, c-jun, akt, and betacellulin gene expression levels were determined by the real-time quantitative reverse transcription-polymerase chain reaction method. |
2021-02-23 |
2023-08-13 |
rat |
| Jiayuan Chen, Li Gao, Yao Zhang, Ya Su, Zheng Kong, Dongqing Wang, Ming Ya. Acteoside-improved streptozotocin-induced learning and memory impairment by upregulating hippocampal insulin, glucose transport, and energy metabolism. Phytotherapy research : PTR. vol 35. issue 1. 2021-02-23. PMID:33029835. |
the acteoside induced increase in the protein expression of glucose transporters (glu t1, glu t3, and glu t4), glucose, and insulin levels in the hippocampus for maintaining normal learning and memory function were demonstrated by western blot. |
2021-02-23 |
2023-08-13 |
Not clear |
| Xiaoqing Yi, Xuan Cai, Sisi Wang, Yanfeng Xia. Mechanisms of impaired pancreatic β‑cell function in high‑fat diet‑induced obese mice: The role of endoplasmic reticulum stress. Molecular medicine reports. vol 21. issue 5. 2021-02-16. PMID:32323766. |
in vitro, ers was induced by palmitate (pa) in ins‑1 cells, and the protein expression of atf6, and mrna expression of atf6 and insulin were examined via western blot and quantitative pcr (qpcr) analyses, respectively. |
2021-02-16 |
2023-08-13 |
mouse |
| Jaison Jeevanandam, Yen San Chan, Michael K Danquah, Ming Chiat La. Cytotoxicity Analysis of Morphologically Different Sol-Gel-Synthesized MgO Nanoparticles and Their In Vitro Insulin Resistance Reversal Ability in Adipose cells. Applied biochemistry and biotechnology. vol 190. issue 4. 2021-01-29. PMID:31776944. |
dns glucose assay and western blot showed that hexagonally shaped mgo nanoparticles had reversed 29.5% of insulin resistance whilst fluorescence microscopy studies indicated that the insulin resistance reversal is due to the activation of intracellular enzymes. |
2021-01-29 |
2023-08-13 |
Not clear |
| Franziska Kopietz, Kaja Rupar, Christine Berggreen, Johanna Säll, Didier Vertommen, Eva Degerman, Mark H Rider, Olga Göransso. Inhibition of AMPK activity in response to insulin in adipocytes: involvement of AMPK pS485, PDEs, and cellular energy levels. American journal of physiology. Endocrinology and metabolism. vol 319. issue 3. 2020-12-30. PMID:32663099. |
ampk activity measurements by western blot analysis and in vitro kinase assay revealed that wt and s485a ampk were inhibited to a similar degree by insulin, indicating that ampk s485 phosphorylation is not required for insulin-induced ampk inhibition. |
2020-12-30 |
2023-08-13 |
human |
| Li Xiong, Yingying Gong, Liting Wu, Jin Li, Weiman He, Xiaonan Zhu, Haipeng Xia. LncRNA-Malat1 is Involved in Lipotoxicity-Induced ß-cell Dysfunction and the Therapeutic Effect of Exendin-4 via Ptbp1. Endocrinology. vol 161. issue 7. 2020-12-14. PMID:32324218. |
the results of tunel, western blot and flow cytometric analyses, and gsis assays revealed that malat1 knockdown significantly induced β-cell apoptosis and inhibited insulin secretion. |
2020-12-14 |
2023-08-13 |
mouse |
| Leilei Yu, Huifeng Wang, Zhongxiu Guo, Fenghua Li, Hong Cu. Role of nucleolar protein NOM1 in pancreatic islet β cell apoptosis in diabetes. Experimental and therapeutic medicine. vol 12. issue 4. 2020-10-01. PMID:27698723. |
furthermore, the mrna expression levels of insulin 1 and 2 in min6 cells were detected using reverse transcription-quantitative polymerase chain reaction, while the expression levels of various cell apoptosis-associated proteins, bcl-2 and bax, were analyzed using western blot analysis. |
2020-10-01 |
2023-08-13 |
mouse |
| Huicun Zhang, Na Ta, Pengmin Chen, Hongbing Wan. Erchen Decoction and Linguizhugan Decoction Ameliorate Hepatic Insulin Resistance by Inhibiting IRS-1Ser307 Phosphorylation In Vivo and In Vitro. Evidence-based complementary and alternative medicine : eCAM. vol 2017. 2020-10-01. PMID:28630632. |
because insulin resistance (ir) is a key etiological factor in the pathology of high-fat diet- (hfd-) induced nafld, in this study, the protective effects of ecd and lgzgd on hfd-induced insulin resistance in rats were evaluated and their mechanisms were investigated by ogtt and western blot. |
2020-10-01 |
2023-08-13 |
rat |
| Ben Niu, Chao Li, Heng Su, Qingzhu Li, Qiu He, Lijuan Liu, Yuanming Xue, Tao Shen, Xueshan Xi. Glucagon-like peptide-1 receptor agonist exendin-4 protects against interleukin-1β-mediated inhibition of glucose-stimulated insulin secretion by mouse insulinoma β cells. Experimental and therapeutic medicine. vol 14. issue 3. 2020-10-01. PMID:28947919. |
cells were stimulated with various concentrations of glucose (0, 1.38, 5.5 and 11.1 mm), and insulin was measured via radioimmunoassay of the supernatant; furthermore, western blot analysis was used to detect phosphorylated extracellular receptor kinase (erk)1/2. |
2020-10-01 |
2023-08-13 |
mouse |
| Naren Tuya, Yadi Wang, Lanmei Tong, Weishi Gao, Rong Yu, Liying Xu. Trichosanthin enhances the antitumor effect of gemcitabine in non-small cell lung cancer via inhibition of the PI3K/AKT pathway. Experimental and therapeutic medicine. vol 14. issue 6. 2020-10-01. PMID:29285119. |
in addition, western blot analysis demonstrated that the combination treatment of tcs with gemz further decreased the expression level of phosphoinositide 3-kinase (pi3k) and akt via regulating the expression of insulin growth factor. |
2020-10-01 |
2023-08-13 |
Not clear |