| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xin Xiao, Wen-Hua Xu, Xiao-Qing Zhang, Jun-Feng Ding, Yue Jiang, Jun T. [Anti-oxidative and anti-apoptotic effects and molecular mechanisms of catalpol against H_2O_2-induced oxidative damage in pancreatic β cells (INS-1 cells)]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 16. 2022-09-01. PMID:36046869. |
the present study investigated the anti-oxidative and anti-apoptotic effects and molecular mechanisms of catalpol on the h_2o_2-induced pancreatic β-cells(ins-1 cells).the oxidative damage model of ins-1 cells was induced and optimized by the stimulation of h_2o_2 of different concentrations for different time.cck-8 assay was used to detect cell viability after catalpol intervention(1, 5, 10, 20, 40, 80, and 160 μmol·l~(-1)) for 24 h.intracellular reactive oxygen species(ros), superoxide dismutase(sod), and lipid peroxide malondialdehyde(mda) were measured by dcfh-da fluorescent probe, wst-1, and tba respectively.moreover, the apo-ptotic effect was detected by ao-eb and annexin v-fitc/pi staining.in addition, the protein expression levels were detected by wes-tern blot, and intracellular insulin concentration was measured by elisa.the results showed that the oxidative damage model of ins-1 cells was stably induced by 50 μmol·l~(-1) h_2o_2 treatment for 2 h, and catalpol at 1-80 μmol·l~(-1) did not affect cell viability of ins-1 cells.compared with the conditions in the model group, 1, 5, and 10 μmol·l~(-1) catalpol intervention for 2 h could protect ins-1 cells from oxidative damage(p<0.001), reduce ros and mda, increase sod, and inhibit excessive cell apoptosis.moreover, 1, 5, and 10 μmol·l~(-1) catalpol could also up-regulate the phosphorylation of nuclear transcription factor nf-e2 related factors, negatively regulate kelch-like ech-associated protein 1(keap1), phosphorylation of extracellular signal-regulated kinase(erk), and heme oxyge-nase 1(ho-1), and promote the protein expression of pancreatic-duodenal homeobox factor-1(pdx-1) and glucose transporter 2(glut2).in addition, 1, 5, and 10 μmol·l~(-1) catalpol increased insulin secretion of ins-1 cells under oxidative damage in the high-glucose culture medium, indicating function recovery of pancreatic β cells.pdx-1 is a key nuclear transcription factor of pancreatic β cell function that directly regulates glut2 and insulin synthesis, and affects glucose homeostasis.in conclusion, catalpol can reduce the oxidative damage and apoptosis of ins-1 cells, activate antioxidant pathway, protect the function of pancreatic β cells, and improve insulin synthesis and secretion. |
2022-09-01 |
2023-08-14 |
Not clear |
| Ya-Fen Pang, Ming Huang, Lin Li, Xin Zhao, Hong-Xia Zhang, Yu-Hong L. [Effect of Danlou Tablets on alleviating hepatic adipogenesis, inflammatory response, and insulin resistance in db/db mice]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 12. 2022-07-19. PMID:35851126. |
western blot was performed to determine the protein levels of insulin receptor-β(irβ), phospho-irβ(p-irβ), phosphatidylinositol 3-kinase(pi3 k), and insulin receptor substrate-1(irs-1) involved in irs-1/pi3 k/akt signaling pathway in the livers of mice to reveal the mechanism of dlt in alleviating insulin resistance in diabetic mice. |
2022-07-19 |
2023-08-14 |
mouse |
| Ruili Yin, Yan Ma, Ning Zhang, Longyan Yang, Dong Zha. Combined effects of voluntary running and liraglutide on glucose homeostasis, fatty acid composition of brown adipose tissue phospholipids, and white adipose tissue browning in db/db mice. The Chinese journal of physiology. vol 65. issue 3. 2022-07-01. PMID:35775530. |
fasting blood glucose was monitored every week, plasma insulin and lipid profiles were analyzed, and thermogenic protein expression in bat and white adipose tissue (wat) were analyzed by the western blot. |
2022-07-01 |
2023-08-14 |
mouse |
| Xiangjuan Wei, Daqian Yang, Boya Zhang, Xingpei Fan, Haining Du, Ruijiao Zhu, Xiaotong Sun, Meimei Zhao, Ning G. Di-(2-ethylhexyl) phthalate increases plasma glucose and induces lipid metabolic disorders via FoxO1 in adult mice. The Science of the total environment. 2022-06-24. PMID:35750186. |
western blot and real-time quantitative pcr showed that dehp induced insulin resistance and promoted gluconeogenesis and lipid accumulation via overexpression of forkhead box protein o1 (foxo1), in keeping with hepatic rna sequencing data. |
2022-06-24 |
2023-08-14 |
mouse |
| Lushan Wang, Timothy S Wiedmann, Karunya K Kandimall. Modulating Insulin Signaling and Trafficking at the Blood-Brain Barrier Endothelium Using Lipid Based Nanoemulsions. International journal of pharmaceutics. 2022-05-23. PMID:35605891. |
moreover, western blot and flow cytometry analysis showed that snes containing polyunsaturated fatty acids (pufas) increased phospo-akt (p-akt) expression, a marker for the stimulation of metabolic arm of the insulin signaling, and insulin uptake in human cerebral microvascular endothelial cell (hcmec/d3) monolayers. |
2022-05-23 |
2023-08-13 |
human |
| Lian-Zhi Cheng, Jia-Mei Zhou, Jun-Long Ma, Fan-Jing Wang, Kai Cheng, Qian Chen, Hui-Lun Yuan, Ai-Juan Jian. [Tetrahydropalmatine alleviated diabetic neuropathic pain by inhibiting activation of microglia via p38 MAPK signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 9. 2022-05-09. PMID:35531701. |
the protein expression levels of ox42, inducible nitric oxide synthase(inos), cd206, p38, and p-p38 were determined by western blot, the fluorescence expression levels of ox42 and p-p38 in the dorsal horn of the rat spinal cord by immunofluorescence, the mrna content of p38 and ox42 in rat spinal cord tissue by qrt-pcr, and levels of nitric oxide(no), interleukin-1β(il-1β), interleukin-6(il-6), tumor necrosis factor-α(tnf-α), interleukin-10(il-10), and serum fasting insulin(fins) by enzyme-linked immunosorbent assay(elisa). |
2022-05-09 |
2023-08-13 |
rat |
| Maryam Bazrgar, Pariya Khodabakhsh, Leila Dargahi, Fatemeh Mohagheghi, Abolhassan Ahmadian. MicroRNA modulation is a potential molecular mechanism for neuroprotective effects of intranasal insulin administration in amyloid βeta oligomer induced Alzheimer's like rat model. Experimental gerontology. 2022-04-27. PMID:35476966. |
then, molecular and histological changes in response to aβo and/or insulin time course were assessed in the extracted hippocampi on days 1, 14, and 21 of the study using congo red staining, western blot and quantitative real-time pcr analyses. |
2022-04-27 |
2023-08-13 |
rat |
| Yaru Li, Xueqin Chen, Yulu Chen, Dongsheng Yu, Ran Jiang, Xinhui Kou, Liang Sheng, Yang Liu, Yu Son. Berberine Improves TNF-α-Induced Hepatic Insulin Resistance by Targeting MEKK1/MEK Pathway. Inflammation. 2022-04-23. PMID:35460012. |
western blot and immunoprecipitation were used to investigate the effect of bbr on the crosstalk between tnf-α pathway and insulin signaling pathway. |
2022-04-23 |
2023-08-13 |
Not clear |
| Seong-Hyun Kim, Sang-Seo Park, Chang-Ju Kim, Tae-Woon Ki. Exercise with 40-Hz light flicker improves hippocampal insulin signaling in Alzheimer disease mice. Journal of exercise rehabilitation. vol 18. issue 1. 2022-03-31. PMID:35356135. |
to investigate insulin signaling proteins, western blot was conducted to detect the ratio of phosphorylated insulin receptor β (p-irβ)/total irβ (t-irβ), phosphorylated insulin receptor substrate 1 (p-irs-1)/total irs-1 (t-irs-1), phosphorylated phosphatidylinositide-3-kinase (p-pi3k)/total pi3k (t-pi3k), phosphorylated 3-phosphoinositide dependent protein kinase-1 (p-pdk1)/total pdk-1 (t-pdk1), phosphorylated protein kinase b (p-akt)/total-akt (t-akt), and phosphorylated glycogen synthase kinase 3 beta (p-gsk3β)/total gsk3β (t-gsk3β). |
2022-03-31 |
2023-08-13 |
mouse |
| Quan-Cao Zhan, Yu-Zhe Liu, Yu-Meng Xi, Yan Zeng, Jia-Yu Tian, Li Liu, Dong Li, Yamahara Johji, Jian-Wei Wan. [6-Gingerol ameliorates adipose tissue insulin resistance in aging rats]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 6. 2022-03-29. PMID:35347963. |
the gene and protein expression levels of adiponectin receptor 1(adipor1), amp-activated protein kinase α(ampkα), phosphorylated ampk(p-ampkα~(thr172)), peroxisome proliferator-activated receptor-γ coactivator-1α(pgc-1α), phosphatidylinositol 3-kinase(pi3 k), protein kinase b(akt), phosphorylated akt(p-akt~(ser473)), tumor necrosis factor-α(tnf-α), c-jun n-terminal kinase 1/2(jnk1/2), phosphorylated jnk1/2(p-jnk~(thr183/tyr185)), interleukin-1β(il-1β), and interleukin-6(il-6) in adiponectin(apn), insulin, and inflammatory factor signaling pathways were detected by western blot and real-time rcr, respectively. |
2022-03-29 |
2023-08-13 |
rat |
| Jia-Luo Cai, Xiao-Ping Li, Yi-Lin Zhu, Gui-Ming Deng, Lei Yang, Xin-Hua Xia, Gang-Qiang Yi, Xin-Yu Che. [Mechanism of Huangjing Qianshi Decoction in treatment of prediabetes based on network pharmacology and molecular docking]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 4. 2022-03-14. PMID:35285205. |
western blot result showed that hqd-containing serum significantly reduced the expression of akt1, age, and rage proteins in insulin resistance model cells. |
2022-03-14 |
2023-08-13 |
Not clear |
| Yongtao Li, Wenshan Bian, Yang Jiang, Danyang Liu, Lei She. hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment. Journal of healthcare engineering. vol 2022. 2022-01-17. PMID:35035844. |
the results of western blot showed that the expression of p53 was significantly decreased in the coculture group, and the glucose stimulated insulin secretion test showed that insulin secretion was significantly increased. |
2022-01-17 |
2023-08-13 |
human |
| Min Hu, Yuehui Zhang, Lingjing Lu, Yu Zhou, Denghui Wu, Mats Brännström, Linus R Shao, Håkan Billi. Overactivation of the androgen receptor exacerbates gravid uterine ferroptosis via interaction with and suppression of the NRF2 defense signaling pathway. FEBS letters. 2022-01-17. PMID:35038776. |
the mechanisms through which the androgen-dependent activation of the androgen receptor (ar) regulates gravid uterine ferroptosis remain unknown. we show that while co-exposure of pregnant rats to the androgen 5α-dihydrotestosterone (dht) and insulin (ins) triggered uterine ferroptotic signaling cascades, additional treatment with the anti-androgen flutamide increased expression of the key ferroptosis-inhibitory proteins slc7a11, gsh, and gpx4, reduced iron content, normalized levels of ferroptosis-associated tfrc, fpn1, and ho1 mrnas, reduced levels of proteins modified by 4-hne (a marker of ferroptosis), and restored protein levels of nrf2, a key transcription factor regulating antioxidant defense signaling, in the gravid uterus. furthermore, exposure to dht alone increased uterine ferroptosis, and nrf2 abundance was negatively correlated with ar status. co-immunoprecipitation and western blot assays revealed that the ar physically interacted with endogenous nrf2, and this interaction was increased by dht exposure in vivo. our results suggest that ar overactivation and nrf2 suppression cooperate in the regulation of nrf2-targets in uterine ferroptosis. |
2022-01-17 |
2023-08-13 |
rat |
| Susana Cardoso, Paula I Moreir. Insulin-Induced Recurrent Hypoglycemia Up-Regulates Glucose Metabolism in the Brain Cortex of Chemically Induced Diabetic Rats. International journal of molecular sciences. vol 22. issue 24. 2021-12-24. PMID:34948265. |
western blot analysis was used to determine the protein content of glucose and monocarboxylate transporters, players in the insulin signaling pathway and mitochondrial biogenesis and dynamics. |
2021-12-24 |
2023-08-13 |
human |
| Katarzyna Hodun, Klaudia Sztolsztener, Adrian Chabowsk. Antioxidants Supplementation Reduces Ceramide Synthesis Improving the Cardiac Insulin Transduction Pathway in a Rodent Model of Obesity. Nutrients. vol 13. issue 10. 2021-11-29. PMID:34684414. |
the proteins expressed from sphingolipid and insulin signaling pathways were determined by western blot. |
2021-11-29 |
2023-08-13 |
rat |
| Xuan Zhou, Jia-Qi Li, Li-Jie Wei, Meng-Zhou He, Jing Jia, Jing-Yi Zhang, Shao-Shuai Wang, Ling Fen. Silencing of DsbA-L gene impairs the PPARγ agonist function of improving insulin resistance in a high-glucose cell model. Journal of Zhejiang University. Science. B. vol 21. issue 12. 2021-11-19. PMID:33843164. |
the western blot technique was performed to verify the relationship between pparγ agonists and dsba-l, and to explore changes in key molecules of the insulin signaling pathway, as well as the effect of chemerin on dsba-l. |
2021-11-19 |
2023-08-13 |
Not clear |
| Dahae Lee, Young-Mi Kim, Hyun Woo Kim, You-Kyoung Choi, Bang Ju Park, Sang Hoon Joo, Ki Sung Kan. Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells and Glucose Uptake in Skeletal Muscle Cells. Molecules (Basel, Switzerland). vol 26. issue 21. 2021-11-18. PMID:34770916. |
in addition, western blot analysis showed that the expression of associated proteins, including peroxisome proliferator-activated receptor γ (pparγ), pancreatic and duodenal homeobox 1 (pdx-1), phosphatidylinositol 3-kinase (pi3k), akt, and insulin receptor substrate-2 (irs-2), was increased in ins-1 cells after treatment with schisandrin c. in addition, insulin secretion effect of schisandrin c were enhanced by the bay k 8644 (l-type ca |
2021-11-18 |
2023-08-13 |
mouse |
| Saltuk Bugra Baltaci, Omer Unal, Elif Gulbahce-Mutlu, Haluk Gumus, Suray Pehlivanoglu, Ahmet Yardimci, Rasim Mogulkoc, Abdulkerim Kasim Baltac. The Role of Zinc Status on Spatial Memory, Hippocampal Synaptic Plasticity, and Insulin Signaling in icv-STZ-Induced Sporadic Alzheimer's-Like Disease in Rats. Biological trace element research. 2021-11-02. PMID:34727320. |
the expression of hippocampal protein and mrna levels of targets related to synaptic plasticity and insulin pathway was assessed by western blot and real-time quantitative pcr. |
2021-11-02 |
2023-08-13 |
rat |
| Hin Ting Wan, Lok Yi Cheung, Ting Fung Chan, Marco Li, Keng Po Lai, Chris Kong Chu Won. Characterization of PFOS toxicity on in-vivo and ex-vivo mouse pancreatic islets. Environmental pollution (Barking, Essex : 1987). vol 289. 2021-09-29. PMID:34330010. |
using the mouse β-cells (min-6) treated with 1 μm and 10 μm pfos for 24 h, western blot analysis consistently showed the pfos-treatment inhibited akt-pathway and reduced cellular insulin contents. |
2021-09-29 |
2023-08-13 |
mouse |
| Waseem El-Huneidi, Shabana Anjum, Khuloud Bajbouj, Eman Abu-Gharbieh, Jalal Taneer. The Coffee Diterpene, Kahweol, Ameliorates Pancreatic β-Cell Function in Streptozotocin (STZ)-Treated Rat INS-1 Cells through NF-kB and p-AKT/Bcl-2 Pathways. Molecules (Basel, Switzerland). vol 26. issue 17. 2021-09-23. PMID:34500601. |
furthermore, western blot analysis showed that kahweol downregulated stz-induced nuclear factor kappa b (nf-κb), and the antioxidant proteins, heme oxygenase-1 (hmox-1), and inhibitor of dna binding and cell differentiation (id) proteins (id1, id3) while upregulated protein expression of insulin (ins), p-akt and b-cell lymphoma 2 (bcl-2). |
2021-09-23 |
2023-08-13 |
rat |