| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jiangqiang Chen, Zuhui Che. Downregulation of miR-19a inhibits the proliferation and promotes the apoptosis of osteosarcoma cells by regulating the JAK2/STAT3 pathway. Oncology letters. vol 20. issue 5. 2020-09-28. PMID:32934740. |
next, the cell proliferation and apoptosis levels in each group were detected via methyl thiazolyl tetrazolium (mtt) assay and flow cytometry, respectively, and the level of reactive oxygen species (ros) in cells was further determined. |
2020-09-28 |
2023-08-13 |
Not clear |
| Wei Wang, Jin-Zhuo Ning, Zhi-Gang Tang, Ying He, Li-Chao Yao, Lin Ye, Lun W. MicroRNA-23a acts as an oncogene in pancreatic carcinoma by targeting TFPI-2. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32952643. |
mtt and flow cytometry assays were used to assess cell viability and apoptosis, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
| Gui-Nan Shen, Jing Li, Ying-Hua Jin, Hu-Nan Sun, Ying-Ying Hao, Mei-Hua Jin, Ren Liu, Wei-Long Li, Yong-Qing Zhang, Jia-Bin Yu, Nan-Nan Yu, Wei-Dong Wang, Li-Yun Yu, Ji-Su Kim, Taeho Kwon, Ying-Hao Ha. The compound 2-benzylthio-5,8-dimethoxynaphthalene-1,4-dione leads to apoptotic cell death by increasing the cellular reactive oxygen species levels in Ras-mutated liver cancer cells. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32968439. |
mtt assays were used to detect cellular viability and flow cytometry was performed to assess intracellular reactive oxygen species (ros) levels and apoptosis. |
2020-09-28 |
2023-08-13 |
mouse |
| Lihong Yang, Xiaojun Huang, Wei Wang, Tao Jiang, Feifei Din. XEDAR inhibits the proliferation and induces apoptosis of gastric cancer cells by regulating JNK signaling pathway. Bioscience reports. vol 39. issue 12. 2020-09-23. PMID:31829409. |
cell proliferation and apoptosis were explored using mtt and annexin-v/propidium iodide (pi) assays, respectively. |
2020-09-23 |
2023-08-13 |
Not clear |
| Bo Zhang, Haijun Zhang, Hao Luo, Chao Yang, Yi Yua. Sinomenine can promote the proliferation and differentiation of osteoblasts by regulating the Akt/Runx2 signaling pathway in MC3T3-E1 cells. Die Pharmazie. vol 74. issue 12. 2020-09-23. PMID:31907116. |
the proliferation and apoptosis of the cells were determined by mtt assay at 0, 24, 48 and 72 h, and flow cytometry method was used to determine the effect of sinomenine on the apoptosis of mc3t3-e1 cells at 72 h; furthermore, after 72 h, the cell culture supernatants were collected, and the levels of alkaline phosphatase and osteocalcin were examined by enzyme-linked immunosorbent assay (elisa); next, cells were collected, and the expression of type i collagen (col1a1), osteopontin (opn), protein kinase b (akt), runt-related transcription factor 2 (runx2), b-cell lymphoma 2 (bcl-2) and bcl-2-associated x protein (bax) were examined by rt-qpcr and western blot methods. |
2020-09-23 |
2023-08-13 |
mouse |
| Rong Luo, Han Jin, Lan Li, Yu-Xiang Hu, Fan Xia. Long Noncoding RNA MEG3 Inhibits Apoptosis of Retinal Pigment Epithelium Cells Induced by High Glucose via the miR-93/Nrf2 Axis. The American journal of pathology. vol 190. issue 9. 2020-09-21. PMID:32473920. |
the effect of meg3 and mir-93 on high glucose (hg)-induced apoptosis was detected by mtt and flow cytometry. |
2020-09-21 |
2023-08-13 |
human |
| Qiang Duan, Erwang S. MicroRNA-25 aggravates Aβ1-42-induced hippocampal neuron injury in Alzheimer's disease by downregulating KLF2 via the Nrf2 signaling pathway in a mouse model. Journal of cellular biochemistry. vol 120. issue 9. 2020-09-18. PMID:31144355. |
mtt assay was used to evaluate the proliferative ability and flow cytometry to detect cell cycle distribution and apoptosis. |
2020-09-18 |
2023-08-13 |
mouse |
| Cao Gao, Xiao-Feng He, Qing-Rong Xu, Yan-Jie Xu, Jiang She. Sevoflurane downregulates insulin-like growth factor-1 to inhibit cell proliferation, invasion and trigger apoptosis in glioma through the PI3K/AKT signaling pathway. Anti-cancer drugs. vol 30. issue 7. 2020-09-18. PMID:31305291. |
the effect of sevoflurane on proliferation was evaluated by mtt, and cell migration assay, cell apoptosis, and invasion ability were evaluated by wound-healing assay, cell apoptosis, and transwell assays. |
2020-09-18 |
2023-08-13 |
human |
| H Li, M W Liu, W Yang, L J Wan, H L Yan, J C Li, S Y Tang, Y Q Wan. Naringenin induces neuroprotection against homocysteine-induced PC12 cells via the upregulation of superoxide dismutase 1 expression by decreasing miR-224-3p expression. Journal of biological regulators and homeostatic agents. vol 34. issue 2. 2020-09-16. PMID:32515177. |
the relative survival rate and activities of the pc12 cells were determined by the mtt method, and the apoptosis rate of the pc12 cells was determined by using flow cytometry. |
2020-09-16 |
2023-08-13 |
Not clear |
| Yuanyuan Ding, Weibo Niu, Tao Zhang, Jue Wang, Jiao Cao, He Chen, Ruiqi Wang, Hongli A. Levistolide A synergistically enhances doxorubicin‑induced apoptosis of k562/dox cells by decreasing MDR1 expression through the ubiquitin pathway. Oncology reports. vol 41. issue 2. 2020-09-11. PMID:30483812. |
the present study conducted the following experiments: mtt assay, apoptosis analysis, cellular doxorubicin accumulation assay, immunoblotting and reverse transcription‑quantitative polymerase chain reaction, to investigate whether levistolide a enhance doxorubicin‑induced apoptosis of k562/dox cells and to determine the molecular mechanisms involved. |
2020-09-11 |
2023-08-13 |
Not clear |
| Miao Guo, Jing-Jing Zhou, Wei Huan. Metformin alleviates endometrial hyperplasia through the UCA1/miR‑144/TGF‑β1/AKT signaling pathway. International journal of molecular medicine. vol 45. issue 2. 2020-09-11. PMID:31894313. |
in addition, mtt and flow cytometry assays were performed to detect the effect of met on cell proliferation and apoptosis. |
2020-09-11 |
2023-08-13 |
Not clear |
| Xiaochun Jin, Hui Wang, Shuzhou Yin, Youtao Zhan. MicroRNA-19a mediates neuroprotection through the PTEN/AKT pathway in SK-N-SH cells after oxygen-glucose deprivation/reoxygenation injury. General physiology and biophysics. vol 39. issue 3. 2020-09-11. PMID:32525819. |
in addition, cell viability and apoptosis were measured using an mtt and flow cytometry. |
2020-09-11 |
2023-08-13 |
Not clear |
| Lei Wang, Mingxin Yu, Shanshan Zha. lncRNA MEG3 modified epithelial-mesenchymal transition of ovarian cancer cells by sponging miR-219a-5p and regulating EGFR. Journal of cellular biochemistry. vol 120. issue 10. 2020-09-09. PMID:31161607. |
moreover, pcdna3.1-meg3, si-meg3, mir-219a-5p mimic, mir-219a-5p inhibitor, pcdna3.1-egfr, and si-egfr were, respectively, transfected into the oc cells, and their impacts on viability, proliferation, apoptosis, invasion, and migration of oc cells were assessed via conduction of mtt assay, colony formation assay, flow cytometry assay, transwell assay, and scratch assay. |
2020-09-09 |
2023-08-13 |
human |
| Dan Yang, Meijuan Zhang, Yu Gan, Si Yang, Jinglei Wang, Mei Yu, Jie Wei, Jiaxiang Che. Involvement of oxidative stress in ZnO NPs-induced apoptosis and autophagy of mouse GC-1 spg cells. Ecotoxicology and environmental safety. vol 202. 2020-09-07. PMID:32800232. |
herein, mouse-derived spermatogonia cell line gc-1 spg cells were treated with zno nps for 24 h in the presence or absence of radical scavenger n-acetyl-l-cysteine (nac) or autophagy inhibitor 3-methyladenine (3-ma), then cell viability was observed by mtt assay; apoptosis was observed by western blotting analysis and annexinv-fitc/pi assay, respectively; autophagy was detected by western blotting analysis and transmission electron microscopy, respectively; and the contents of mda and gsh and the activities of sod and gsh-px were measured by oxidative stress kits. |
2020-09-07 |
2023-08-13 |
mouse |
| Yun Shi, Ya Su, Chuanli Liu, Jinglong Tao, Hao Fu, Chengzhi L. Inhibition of HES-1 might play a protective role in endothelial cells under cholesterol stimulation via PI3K/AKT signaling pathway. General physiology and biophysics. vol 39. issue 2. 2020-09-03. PMID:32329442. |
in this study, we established a cell model of hes-1 inhibition and overexpression in ea.hy 926 cells, and firstly detected the proliferation rete of ea.hy 926 cells under cholesterol stimulation using mtt assay, and apoptosis of ea.hy 926 cells were detected using flow cytometry. |
2020-09-03 |
2023-08-13 |
Not clear |
| Lili Zheng, Jing Huang, Yuan Su, Fang Wang, Hongfang Kong, Hong Xi. Overexpression of tissue factor pathway inhibitor 2 attenuates trophoblast proliferation and invasion in preeclampsia. Human cell. vol 33. issue 3. 2020-09-02. PMID:32130677. |
mtt assay, annexin v/propidium iodide (pi) apoptosis assay, and transwell assay were used to determine the cell proliferation, apoptosis, and invasion. |
2020-09-02 |
2023-08-13 |
Not clear |
| Daniela Carneiro de Lima, Silvana Vianna Rodrigues, Gilson Teles Boaventura, Hee-Yeon Cho, Thomas C Chen, Axel H Schönthal, Clovis Orlando Da Fonsec. Simultaneous measurement of perillyl alcohol and its metabolite perillic acid in plasma and lung after inhalational administration in Wistar rats. Drug testing and analysis. vol 12. issue 2. 2020-09-01. PMID:31800149. |
toxicity was established by a standard mtt assay, and apoptosis markers were analyzed by western blot. |
2020-09-01 |
2023-08-13 |
rat |
| Zheyu Zhang, Sifang Zhang, Jingjing Yang, Pengji Yi, Panpan Xu, Min Yi, Weijun Pen. Integrated transcriptomic and metabolomic analyses to characterize the anti-cancer effects of (-)-epigallocatechin-3-gallate in human colon cancer cells. Toxicology and applied pharmacology. vol 401. 2020-08-31. PMID:32512070. |
the mtt assay, flow cytometry, and tunel staining were used to study the underlying mechanisms of egcg (proliferation, apoptosis, autophagy). |
2020-08-31 |
2023-08-13 |
human |
| Xinyu Tang, Yang Li, Long Liu, Rui Guo, Ping Zhang, Yihe Zhang, Yong Zhang, Jia Zhao, Jing Su, Liankun Sun, Yanan Li. Sirtuin 3 induces apoptosis and necroptosis by regulating mutant p53 expression in small‑cell lung cancer. Oncology reports. vol 43. issue 2. 2020-08-21. PMID:31894331. |
light microscopy, mtt and flow cytometric assays revealed that sirt3 overexpression inhibited growth and promoted apoptosis in nci‑h446 human small cell lung cancer cells. |
2020-08-21 |
2023-08-13 |
human |
| Zhonghui Chen, Weibing Zhang, Nu Zhang, Yan Zhou, Geliang Hu, Mingdi Xue, Junhua Liu, Yaming L. Down-regulation of insulin-like growth factor binding protein 5 is involved in intervertebral disc degeneration via the ERK signalling pathway. Journal of cellular and molecular medicine. vol 23. issue 9. 2020-08-20. PMID:31290273. |
igfbp5 was shown to significantly promote np cell proliferation and inhibit apoptosis in vitro, which was confirmed by mtt, flow cytometry and colony formation assays. |
2020-08-20 |
2023-08-13 |
human |