| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Qingfen Wang, Rui Li, Zhi Xiao, Cun Ho. Lycopene attenuates high glucose-mediated apoptosis in MPC5 podocytes by promoting autophagy via the PI3K/AKT signaling pathway. Experimental and therapeutic medicine. vol 20. issue 3. 2020-09-28. PMID:32765784. |
mpc5 podocyte viability and apoptosis were assessed by performing the mtt assay and flow cytometry, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
| Shefeng Zheng, Jinnv Liu, Zhangjian Zhao, Ruifang Son. Role of STAT3/mTOR pathway in chronic kidney injury. American journal of translational research. vol 12. issue 7. 2020-09-28. PMID:32774701. |
glomerular mesangial cells were divided into control group, model group, stat3 inhibitor (s3i-201) group followed by analysis of cell proliferation by mtt assay, cell apoptosis by flow cytometry, formation of autophagosomes by electron microscopy, expression of stat3/mtor signaling proteins and autophagy proteins lc3ii and p62 by western blot, expression of e-cadherin and vimentin by immunofluorescence. |
2020-09-28 |
2023-08-13 |
mouse |
| Nguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikaw. Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells. Acta histochemica et cytochemica. vol 53. issue 4. 2020-09-28. PMID:32873990. |
cell viability and apoptosis were examined by mtt assay, flow cytometry and tunel assay for 24 hr after ptpp-pdt. |
2020-09-28 |
2023-08-13 |
human |
| Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Ca. MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/ Gastroenterology research and practice. vol 2020. 2020-09-28. PMID:32904598. |
lentiviral vector pgcsil-shmac30-gfp of the rna interference (rnai) of the mac30 gene was transfected into gastric cancer bgc-823 cell line and the expression of lentivirus label protein gfp was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and mtt assay, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
| Mahdiyeh Moudi, Ramin Saravani, Saman Sargaz. Silencing of Nucleostemin by siRNA Induces Apoptosis in MCF-7 and MDA-MB-468 Cell Lines. Iranian journal of pharmaceutical research : IJPR. vol 19. issue 1. 2020-09-28. PMID:32922467. |
in addition, we investigated the correlation between knockdown of ns and viability rates and apoptosis induction in mcf-7 and mda-mb-468 cell lines using the mtt assay and annexin v/pi staining, respectively. |
2020-09-28 |
2023-08-13 |
human |
| Li Kong, Yang Sun, Maosheng Chen, Yan Dai, Zhen Li. Downregulation of microRNA-320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32934674. |
cell viability and apoptosis were assessed via mtt and flow cytometry analysis, respectively. |
2020-09-28 |
2023-08-13 |
human |
| Li Kong, Yang Sun, Maosheng Chen, Yan Dai, Zhen Li. Downregulation of microRNA-320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32934674. |
the results of mtt and flow cytometry analysis revealed that overexpression of tusc3 reduced the viability of retinoblastoma cells and induced apoptosis. |
2020-09-28 |
2023-08-13 |
human |
| Fu-Ren Yue, Zhi-Bin Wei, Rui-Zhen Yan, Qiu-Hong Guo, Bing Liu, Jing-Hui Zhang, Zheng L. SMYD3 promotes colon adenocarcinoma (COAD) progression by mediating cell proliferation and apoptosis. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32934676. |
colony formation, mtt, fcm assays and animal assays indicated smyd3 affected the proliferation, apoptosis and the cell cycle of coad cells |
2020-09-28 |
2023-08-13 |
human |
| Jiangqiang Chen, Zuhui Che. Downregulation of miR-19a inhibits the proliferation and promotes the apoptosis of osteosarcoma cells by regulating the JAK2/STAT3 pathway. Oncology letters. vol 20. issue 5. 2020-09-28. PMID:32934740. |
next, the cell proliferation and apoptosis levels in each group were detected via methyl thiazolyl tetrazolium (mtt) assay and flow cytometry, respectively, and the level of reactive oxygen species (ros) in cells was further determined. |
2020-09-28 |
2023-08-13 |
Not clear |
| Wei Wang, Jin-Zhuo Ning, Zhi-Gang Tang, Ying He, Li-Chao Yao, Lin Ye, Lun W. MicroRNA-23a acts as an oncogene in pancreatic carcinoma by targeting TFPI-2. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32952643. |
mtt and flow cytometry assays were used to assess cell viability and apoptosis, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
| Gui-Nan Shen, Jing Li, Ying-Hua Jin, Hu-Nan Sun, Ying-Ying Hao, Mei-Hua Jin, Ren Liu, Wei-Long Li, Yong-Qing Zhang, Jia-Bin Yu, Nan-Nan Yu, Wei-Dong Wang, Li-Yun Yu, Ji-Su Kim, Taeho Kwon, Ying-Hao Ha. The compound 2-benzylthio-5,8-dimethoxynaphthalene-1,4-dione leads to apoptotic cell death by increasing the cellular reactive oxygen species levels in Ras-mutated liver cancer cells. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32968439. |
mtt assays were used to detect cellular viability and flow cytometry was performed to assess intracellular reactive oxygen species (ros) levels and apoptosis. |
2020-09-28 |
2023-08-13 |
mouse |
| Lihong Yang, Xiaojun Huang, Wei Wang, Tao Jiang, Feifei Din. XEDAR inhibits the proliferation and induces apoptosis of gastric cancer cells by regulating JNK signaling pathway. Bioscience reports. vol 39. issue 12. 2020-09-23. PMID:31829409. |
cell proliferation and apoptosis were explored using mtt and annexin-v/propidium iodide (pi) assays, respectively. |
2020-09-23 |
2023-08-13 |
Not clear |
| Bo Zhang, Haijun Zhang, Hao Luo, Chao Yang, Yi Yua. Sinomenine can promote the proliferation and differentiation of osteoblasts by regulating the Akt/Runx2 signaling pathway in MC3T3-E1 cells. Die Pharmazie. vol 74. issue 12. 2020-09-23. PMID:31907116. |
the proliferation and apoptosis of the cells were determined by mtt assay at 0, 24, 48 and 72 h, and flow cytometry method was used to determine the effect of sinomenine on the apoptosis of mc3t3-e1 cells at 72 h; furthermore, after 72 h, the cell culture supernatants were collected, and the levels of alkaline phosphatase and osteocalcin were examined by enzyme-linked immunosorbent assay (elisa); next, cells were collected, and the expression of type i collagen (col1a1), osteopontin (opn), protein kinase b (akt), runt-related transcription factor 2 (runx2), b-cell lymphoma 2 (bcl-2) and bcl-2-associated x protein (bax) were examined by rt-qpcr and western blot methods. |
2020-09-23 |
2023-08-13 |
mouse |
| Rong Luo, Han Jin, Lan Li, Yu-Xiang Hu, Fan Xia. Long Noncoding RNA MEG3 Inhibits Apoptosis of Retinal Pigment Epithelium Cells Induced by High Glucose via the miR-93/Nrf2 Axis. The American journal of pathology. vol 190. issue 9. 2020-09-21. PMID:32473920. |
the effect of meg3 and mir-93 on high glucose (hg)-induced apoptosis was detected by mtt and flow cytometry. |
2020-09-21 |
2023-08-13 |
human |
| Qiang Duan, Erwang S. MicroRNA-25 aggravates Aβ1-42-induced hippocampal neuron injury in Alzheimer's disease by downregulating KLF2 via the Nrf2 signaling pathway in a mouse model. Journal of cellular biochemistry. vol 120. issue 9. 2020-09-18. PMID:31144355. |
mtt assay was used to evaluate the proliferative ability and flow cytometry to detect cell cycle distribution and apoptosis. |
2020-09-18 |
2023-08-13 |
mouse |
| Cao Gao, Xiao-Feng He, Qing-Rong Xu, Yan-Jie Xu, Jiang She. Sevoflurane downregulates insulin-like growth factor-1 to inhibit cell proliferation, invasion and trigger apoptosis in glioma through the PI3K/AKT signaling pathway. Anti-cancer drugs. vol 30. issue 7. 2020-09-18. PMID:31305291. |
the effect of sevoflurane on proliferation was evaluated by mtt, and cell migration assay, cell apoptosis, and invasion ability were evaluated by wound-healing assay, cell apoptosis, and transwell assays. |
2020-09-18 |
2023-08-13 |
human |
| H Li, M W Liu, W Yang, L J Wan, H L Yan, J C Li, S Y Tang, Y Q Wan. Naringenin induces neuroprotection against homocysteine-induced PC12 cells via the upregulation of superoxide dismutase 1 expression by decreasing miR-224-3p expression. Journal of biological regulators and homeostatic agents. vol 34. issue 2. 2020-09-16. PMID:32515177. |
the relative survival rate and activities of the pc12 cells were determined by the mtt method, and the apoptosis rate of the pc12 cells was determined by using flow cytometry. |
2020-09-16 |
2023-08-13 |
Not clear |
| Yuanyuan Ding, Weibo Niu, Tao Zhang, Jue Wang, Jiao Cao, He Chen, Ruiqi Wang, Hongli A. Levistolide A synergistically enhances doxorubicin‑induced apoptosis of k562/dox cells by decreasing MDR1 expression through the ubiquitin pathway. Oncology reports. vol 41. issue 2. 2020-09-11. PMID:30483812. |
the present study conducted the following experiments: mtt assay, apoptosis analysis, cellular doxorubicin accumulation assay, immunoblotting and reverse transcription‑quantitative polymerase chain reaction, to investigate whether levistolide a enhance doxorubicin‑induced apoptosis of k562/dox cells and to determine the molecular mechanisms involved. |
2020-09-11 |
2023-08-13 |
Not clear |
| Miao Guo, Jing-Jing Zhou, Wei Huan. Metformin alleviates endometrial hyperplasia through the UCA1/miR‑144/TGF‑β1/AKT signaling pathway. International journal of molecular medicine. vol 45. issue 2. 2020-09-11. PMID:31894313. |
in addition, mtt and flow cytometry assays were performed to detect the effect of met on cell proliferation and apoptosis. |
2020-09-11 |
2023-08-13 |
Not clear |
| Xiaochun Jin, Hui Wang, Shuzhou Yin, Youtao Zhan. MicroRNA-19a mediates neuroprotection through the PTEN/AKT pathway in SK-N-SH cells after oxygen-glucose deprivation/reoxygenation injury. General physiology and biophysics. vol 39. issue 3. 2020-09-11. PMID:32525819. |
in addition, cell viability and apoptosis were measured using an mtt and flow cytometry. |
2020-09-11 |
2023-08-13 |
Not clear |