Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
Na Wang, Yuliang Yuan, Shipeng Sun, Guijian Li. microRNA-204-5p Participates in Atherosclerosis Via Targeting MMP-9. Open medicine (Warsaw, Poland). vol 15. 2020-09-28. PMID:32266319. |
subsequently, thiazolyl blue tetrazolium bromide (mtt) assay, transwell assay and flow cytometry were performed to detect the proliferation, migration and apoptosis of hvsmcs. |
2020-09-28 |
2023-08-13 |
human |
Peng Chen, Zhengchao Shen, Xiaosan Fang, Guannan Wang, Xiaoming Wang, Jun Wang, Shihang X. Silencing of keratin 17 by lentivirus-mediated short hairpin RNA inhibits the proliferation of PANC-1 human pancreatic cancer cells. Oncology letters. vol 19. issue 5. 2020-09-28. PMID:32269627. |
cell proliferation was determined using an mtt assay, in addition to cell cycle distribution and apoptosis analysis using flow cytometry, colony formation assay using giemsa staining and cell motility analysis using a transwell migration assay. |
2020-09-28 |
2023-08-13 |
human |
Dengtao Liu, Ping Han, Chunhai Gao, Wei Gao, Xiaocui Yao, Shulan Li. microRNA-155 Modulates Hepatic Stellate Cell Proliferation, Apoptosis, and Cell Cycle Progression in Rats With Alcoholic Hepatitis Frontiers in pharmacology. vol 11. 2020-09-28. PMID:32317960. |
the biological prediction website microrna.org and dual-luciferase reporter gene assay were used to identify whether socs1 was a direct target of mir-155, and the effects of mir-155 and socs1 on the viability, cycle progression, and apoptosis of hepatic stellate cells were assessed using rt-qpcr, western blot assay, mtt assay, annexin v/pi double staining, and pi single staining. |
2020-09-28 |
2023-08-13 |
rat |
Yi Wang, Yaohui Tian, Zonghao Li, Zhaoke Zheng, Liangliang Zh. miR-92 Regulates the Proliferation, Migration, Invasion and Apoptosis of Glioma Cells by Targeting Neogenin. Open medicine (Warsaw, Poland). vol 15. 2020-09-28. PMID:32318624. |
cell viability and apoptosis were measured by mtt and flow cytometry assays, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
Jindong Li, Ya Ling, Shengnan Yin, Shufang Yang, Min Kong, Zhiqin L. Baicalin serves a protective role in diabetic nephropathy through preventing high glucose-induced podocyte apoptosis. Experimental and therapeutic medicine. vol 20. issue 1. 2020-09-28. PMID:32550886. |
then, the cells were treated with different concentrations of ba (6.25, 12.5 and 25 µm) for 24 h. cell viability and apoptosis were determined using an mtt assay and flow cytometry, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
Shasha Liu, Qiuyue Du, Yang Rao, Caiyan Liu, Pengpeng Q. Long non-coding RNA NPBWR1-2 affects the development of ovarian cancer via multiple microRNAs. Oncology letters. vol 20. issue 1. 2020-09-28. PMID:32565993. |
cell viability, proliferation, migration, invasion and apoptosis were evaluated using mtt, colony-formation, transwell and flow cytometry assays, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
Zhiqiang Zhao, Mansi Wu, Xuelin Zhang, Qinglin Jin, Yongqian Wang, Changye Zou, Gang Huang, Junqiang Yin, Xianbiao Xie, Jingnan She. CB-5083, an inhibitor of P97, suppresses osteosarcoma growth and stem cell properties by altering protein homeostasis. American journal of translational research. vol 12. issue 6. 2020-09-28. PMID:32655822. |
mtt, colony formation, sphere formation, cell cycle and apoptosis assays and animal studies showed that cb-5083 significantly inhibited osteosarcoma cell growth in vitro and in vivo. |
2020-09-28 |
2023-08-13 |
Not clear |
Xinyue Liang, Wen X. miR-181a-5p regulates the proliferation and apoptosis of glomerular mesangial cells by targeting KLF6. Experimental and therapeutic medicine. vol 20. issue 2. 2020-09-28. PMID:32742352. |
subsequently, mtt and flow cytometric assays were performed in order to determine the effect of mir-181a-5p and klf6 on high-glucose-driven gmc proliferation and apoptosis. |
2020-09-28 |
2023-08-13 |
Not clear |
Yufeng Wang, Luo Jie, Haifeng Gong, Yuqin Li, Anxia Xie, Yanjun Li, Hong Gu. miR-30 inhibits proliferation of trophoblasts in preeclampsia rats partially related to MAPK/ERK pathway. Experimental and therapeutic medicine. vol 20. issue 2. 2020-09-28. PMID:32742372. |
the effects of expression level of mir-30 on the proliferation and hypoxia-induced apoptosis of htr8/svneo cells were detected via methyl thiazolyl tetrazolium (mtt) assay and annexin v/propidium iodide staining, respectively, using the flow cytometer. |
2020-09-28 |
2023-08-13 |
rat |
Barbara Strojny, Sławomir Jaworski, Irena Misiewicz-Krzemińska, Isabel Isidro, Elizabeta A Rojas, Norma C Gutiérrez, Marta Grodzik, Piotr Koczoń, André Chwalibog, Ewa Sawos. Effect of Graphene Family Materials on Multiple Myeloma and Non-Hodgkin's Lymphoma Cell Lines. Materials (Basel, Switzerland). vol 13. issue 15. 2020-09-28. PMID:32756412. |
we performed two types of viability assays, mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide conversion) and atp (adenosine triphosphate detection), flow cytometry analysis of apoptosis induction and cell cycle, cell morphology, and direct interaction analysis using two approaches-visualization of living cells by two different systems, and visualization of fixed and dyed cells. |
2020-09-28 |
2023-08-13 |
Not clear |
Meng Li, Yibin Fan, Yutong Wang, Jinhua Xu, Hui X. ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo. Experimental and therapeutic medicine. vol 20. issue 2. 2020-09-28. PMID:32765670. |
zmiz1 overexpression and knockdown pig1 and pig3v cell models were established by lentivirus infection, and the effects of zmiz1 on cell proliferation and apoptosis were determined using an mtt assay and flow cytometry, respectively. |
2020-09-28 |
2023-08-13 |
human |
Xiaozhi Deng, Lihong Shang, Min Du, Lan Yuan, Lijing Xiong, Xiaoli Xi. Mechanism underlying the significant role of the miR-4262/SIRT1 axis in children with inflammatory bowel disease. Experimental and therapeutic medicine. vol 20. issue 3. 2020-09-28. PMID:32765699. |
mtt assay, flow cytometry and western blot analysis were performed to assess cell viability, apoptosis and nf-κb signaling pathway-related protein levels, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
Qingfen Wang, Rui Li, Zhi Xiao, Cun Ho. Lycopene attenuates high glucose-mediated apoptosis in MPC5 podocytes by promoting autophagy via the PI3K/AKT signaling pathway. Experimental and therapeutic medicine. vol 20. issue 3. 2020-09-28. PMID:32765784. |
mpc5 podocyte viability and apoptosis were assessed by performing the mtt assay and flow cytometry, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
Shefeng Zheng, Jinnv Liu, Zhangjian Zhao, Ruifang Son. Role of STAT3/mTOR pathway in chronic kidney injury. American journal of translational research. vol 12. issue 7. 2020-09-28. PMID:32774701. |
glomerular mesangial cells were divided into control group, model group, stat3 inhibitor (s3i-201) group followed by analysis of cell proliferation by mtt assay, cell apoptosis by flow cytometry, formation of autophagosomes by electron microscopy, expression of stat3/mtor signaling proteins and autophagy proteins lc3ii and p62 by western blot, expression of e-cadherin and vimentin by immunofluorescence. |
2020-09-28 |
2023-08-13 |
mouse |
Nguyen Nhat Huynh Mai, Yuya Yamaguchi, Narantsog Choijookhuu, Jin Matsumoto, Atsushi Nanashima, Hideaki Takagi, Katsuaki Sato, Le Quoc Tuan, Yoshitaka Hishikaw. Photodynamic Therapy Using a Novel Phosphorus Tetraphenylporphyrin Induces an Anticancer Effect via Bax/Bcl-xL-related Mitochondrial Apoptosis in Biliary Cancer Cells. Acta histochemica et cytochemica. vol 53. issue 4. 2020-09-28. PMID:32873990. |
cell viability and apoptosis were examined by mtt assay, flow cytometry and tunel assay for 24 hr after ptpp-pdt. |
2020-09-28 |
2023-08-13 |
human |
Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Ca. MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/ Gastroenterology research and practice. vol 2020. 2020-09-28. PMID:32904598. |
lentiviral vector pgcsil-shmac30-gfp of the rna interference (rnai) of the mac30 gene was transfected into gastric cancer bgc-823 cell line and the expression of lentivirus label protein gfp was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and mtt assay, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
Mahdiyeh Moudi, Ramin Saravani, Saman Sargaz. Silencing of Nucleostemin by siRNA Induces Apoptosis in MCF-7 and MDA-MB-468 Cell Lines. Iranian journal of pharmaceutical research : IJPR. vol 19. issue 1. 2020-09-28. PMID:32922467. |
in addition, we investigated the correlation between knockdown of ns and viability rates and apoptosis induction in mcf-7 and mda-mb-468 cell lines using the mtt assay and annexin v/pi staining, respectively. |
2020-09-28 |
2023-08-13 |
human |
Li Kong, Yang Sun, Maosheng Chen, Yan Dai, Zhen Li. Downregulation of microRNA-320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32934674. |
cell viability and apoptosis were assessed via mtt and flow cytometry analysis, respectively. |
2020-09-28 |
2023-08-13 |
human |
Li Kong, Yang Sun, Maosheng Chen, Yan Dai, Zhen Li. Downregulation of microRNA-320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32934674. |
the results of mtt and flow cytometry analysis revealed that overexpression of tusc3 reduced the viability of retinoblastoma cells and induced apoptosis. |
2020-09-28 |
2023-08-13 |
human |
Fu-Ren Yue, Zhi-Bin Wei, Rui-Zhen Yan, Qiu-Hong Guo, Bing Liu, Jing-Hui Zhang, Zheng L. SMYD3 promotes colon adenocarcinoma (COAD) progression by mediating cell proliferation and apoptosis. Experimental and therapeutic medicine. vol 20. issue 5. 2020-09-28. PMID:32934676. |
colony formation, mtt, fcm assays and animal assays indicated smyd3 affected the proliferation, apoptosis and the cell cycle of coad cells |
2020-09-28 |
2023-08-13 |
human |