| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Si-Ning Shen, Ke Li, Ying Liu, Cheng-Liang Yang, Chun-Yu He, Hao-Rang Wan. Silencing lncRNAs PVT1 Upregulates miR-145 and Confers Inhibitory Effects on Viability, Invasion, and Migration in EC. Molecular therapy. Nucleic acids. vol 19. 2021-12-02. PMID:31951853. |
scratch test, transwell assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, and flow cytometry were performed for detection of migration, invasion, viability, and apoptosis of transfected cells, respectively. |
2021-12-02 |
2023-08-13 |
mouse |
| Wei Wang, Liang Ge, Lili Zhang, Lin Liu, Xuehong Zhang, Xiaoling M. MicroRNA-16 represses granulosa cell proliferation in polycystic ovarian syndrome through inhibition of the PI3K/Akt pathway by downregulation of Apelin13. Human fertility (Cambridge, England). 2021-12-02. PMID:34854361. |
mtt assay was used to detect cell viability, clone formation assay to detect cell proliferation, and flow cytometry to detect cell apoptosis rate. |
2021-12-02 |
2023-08-13 |
Not clear |
| Meixue Chen, Jing Li, Jinfeng Wang, Yuan Le, Chunfeng Li. SMYD1 alleviates septic myocardial injury by inhibiting endoplasmic reticulum stress. Bioscience, biotechnology, and biochemistry. vol 85. issue 12. 2021-12-01. PMID:34601561. |
mtt assay, ldh and ck-mb release assay, flow cytometry, and elisa assay showed that smyd1 overexpression enhanced cell viability, alleviated cell injury, impeded apoptosis, and reduced the level of proinflammatory factors and nf-κb activation under the condition of lps stimulation. |
2021-12-01 |
2023-08-13 |
rat |
| Liu Gang, Li Qun, Wei-Dong Liu, Yong-Sheng Li, Yao-Zeng Xu, Dong-Tang Yua. MicroRNA-34a promotes cell cycle arrest and apoptosis and suppresses cell adhesion by targeting DUSP1 in osteosarcoma. American journal of translational research. vol 9. issue 12. 2021-11-30. PMID:29312491. |
cells proliferation, cell cycle and apoptosis was measured by mtt and flow cytometry assay. |
2021-11-30 |
2023-08-13 |
Not clear |
| Wander Lopes Pereira, Tânia Toledo de Oliveira, Milton Masahiko Kanashiro, Marcelo Augusto Filardi, Marcelo Rocha da Costa, Liovando Marciano da Cost. Morin exhibits leukemic cellular apoptosis through caspase pathway. Natural product research. vol 35. issue 23. 2021-11-30. PMID:32674703. |
the treatments were evaluated by using the mtt and ldh assays; analysis of mitochondrial membrane potential (ΔΨm) was evaluated by flow cytometry, cell death by apoptosis was confirmed by fluorescence microscopy and by assessing the activity of caspases-3 and -6. |
2021-11-30 |
2023-08-13 |
human |
| Lei Gong, Juan Chen, Xijiao Jian. Circ_0005033 is an oncogene in laryngeal squamous cell carcinoma and regulates cell progression and Cisplatin sensitivity via miR-107/IGF1R axis. Anti-cancer drugs. 2021-11-30. PMID:34845162. |
functional assays were performed using mtt assay, edu assay, apoptosis assay, flow cytometry, transwell assay, and xenograft tumor model. |
2021-11-30 |
2023-08-13 |
Not clear |
| Qiuru Che, Liwei You, Yumeng Dai, Wei Sun, Tao Wang, Ke Ding, Yunfei Li, Yuqiang Zhang, Linlin Ding, Xingxing Wang, Zhuoqi Zhang, Zhiwei Li, Liquan Yan. Pleiotrophin affects the susceptibility of prostate cancer cells to cisplatin. Anti-cancer drugs. 2021-11-30. PMID:34845163. |
viability and apoptosis of pc3 cells treated with different concentrations of cisplatin in the presence or absence of purified pleiotrophin were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay and flow cytometry, respectively. |
2021-11-30 |
2023-08-13 |
Not clear |
| Sen Li, Faqing Xie, Kaiwen Shi, Jin Wang, Yan Cao, Yongxiang L. Gossypol ameliorates the IL-1β-induced apoptosis and inflammation in chondrocytes by suppressing the activation of TLR4/MyD88/NF-κB pathway via downregulating CX43. Tissue & cell. vol 73. 2021-11-29. PMID:34534743. |
in treated chondrocytes, according to mtt assay and flow cytometry, gossypol increased viability and reduced apoptosis of il-1β induced chondrocytes. |
2021-11-29 |
2023-08-13 |
Not clear |
| Maryam Mogadasi, Ali Mohammadi, Behnam Emamgolizadeh, Mohammad Reza Alivand, Dara Rahmanpour, Saeed Solal. Investigation the Cytotoxicity of 5-AZA on Acute Lymphoblastic Leukemia Cell Line In Vitro and Characterization the Underlying Molecular Mechanisms of Cell Death and Motility. Asian Pacific journal of cancer prevention : APJCP. vol 22. issue 11. 2021-11-28. PMID:34837933. |
the effect of the 5-aza treatment on cell viability (mtt assay), apoptosis (annexin v/pi staining), microrna (mirna) and mrna expression (real-time pcr) was measured. |
2021-11-28 |
2023-08-13 |
human |
| Xin Zhang, He Nan, Jialong Guo, Jinyu Li. KDM4B Overexpression Promotes the Growth, Migration, and Invasion of Rheumatoid Arthritis Fibroblast-Like Synoviocytes by Activating STAT3 Pathway. Biochemical genetics. vol 59. issue 6. 2021-11-27. PMID:33909202. |
the effects of kdm4b on ra fls viability, apoptosis, migration, and invasion were detected by mtt, flow cytometry, transwell migration, and invasion assays. |
2021-11-27 |
2023-08-13 |
Not clear |
| Zheng Qi, Zhiwei Le, Furui Han, Yajie Feng, Ming Yang, Chenfeng Ji, Liangliang Zha. Inhibitory regulation of purple sweet potato polysaccharide on the hepatotoxicity of tri-(2,3-dibromopropyl) isocyanate. International journal of biological macromolecules. 2021-11-27. PMID:34813788. |
the effect of pspp on the apoptosis of hepg2 cells was detected by mtt assay, the morphological changes were observed by morphological observation, and the apoptosis rate was determined by flow cytometry. |
2021-11-27 |
2023-08-13 |
Not clear |
| Zahra Yazdani, Pooyan Mehrabanjoubani, Alireza Rafiei, Pourya Biparva, Mostafa Karda. Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer. BioMed research international. vol 2021. 2021-11-27. PMID:34825002. |
here, we investigated the combined effects of cap and cur on cytotoxicity and apoptosis in b16-f10 melanoma cancer cells compared to l929 normal cells using mtt method, acridine orange/ethidium bromide fluorescence microscopic assay, and annexin v/pi flow cytometry. |
2021-11-27 |
2023-08-13 |
Not clear |
| Feixiang Chen, Jiadong Zhu, Wei Wan. Ulinastatin Attenuates LPS-Induced Inflammation and Inhibits Endoplasmic Reticulum Stress-Induced Apoptosis in Renal Tubular Epithelial Cells via Regulation of the TLR4/NF-κB and Nrf2/HO-1 Pathways. Inflammation. vol 44. issue 6. 2021-11-26. PMID:34585338. |
this study was intended to explore the underlying mechanism by which ulinastatin (uti) influenced the inflammation and apoptosis of renal tubular epithelial cells, hk-2.the effects of uti on the cell viability of hk-2 cells were first measured by mtt and lactate dehydrogenase (ldh) detection kit. |
2021-11-26 |
2023-08-13 |
Not clear |
| Wei-Wei Guo, Ming-Ming Feng, Shuang-Feng Li, Li-Hui We. Circular RNA circ_0023404 serves as a miR-636 sponge to promote malignant behaviors in cervical cancer cells through upregulation of CYP2S1. The Kaohsiung journal of medical sciences. 2021-11-26. PMID:34825467. |
cell proliferation, migration, invasion, and apoptosis were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-h-tetrazolium bromide (mtt) assay, 5-ethynyl-2'-deoxyuridine (edu) assay, colony formation assay, transwell assay, and cytometry assay. |
2021-11-26 |
2023-08-13 |
Not clear |
| Lei Yang, You-Ling Shi, Yan Ma, Wei-Wei Ren, Guang-Ming Pang, Jiao Li. Silencing KLF16 inhibits oral squamous cell carcinoma cell proliferation by arresting the cell cycle and inducing apoptosis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2021-11-25. PMID:34779529. |
subsequently, cell proliferation was detected using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (mtt) assays, cell migration and invasion were detected with wound healing and transwell assays, and cell cycle distribution and cell apoptosis were detected via flow cytometry. |
2021-11-25 |
2023-08-13 |
mouse |
| Junzhe Li, Shijie Xu, Hua Dong, Xiayu Wu, Li-Hong Wang, Xianhua X. Altered Wnt5a expression affects radiosensitivity of non-small cell lung cancer via the Wnt/β-catenin pathway. Experimental and therapeutic medicine. vol 23. issue 1. 2021-11-25. PMID:34815757. |
in the present study, mtt assay and flow cytometric analysis were performed to assess the effect of overexpression or knockdown of wnt5a and/or radiation on the proliferation and apoptosis of nsclc cells. |
2021-11-25 |
2023-08-13 |
Not clear |
| Peijun Sun, Miao Ni. Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis. Cellular and molecular biology (Noisy-le-Grand, France). vol 67. issue 2. 2021-11-25. PMID:34817332. |
in this study, the rat pancreatic acini ar42j cells were chosen as the research object, the application of hyla induced pancreatic acinar cells made model for acute pancreatitis, application of different concentrations of angelica peony spread effect on building cells, thus divided into control group, built in the module, the low concentration group, concentration and high concentration groups, determined by mtt method was applied to explore the above categories in cell proliferation, cell apoptosis was measured by flow cytometry, the expression of inflammatory factors in cell supernatant was determined by enzyme-linked immunoassay, and the expression of autophagy marker proteins lc3- ? |
2021-11-25 |
2023-08-13 |
rat |
| Bing Wen, Chenjiang He, Qin Zhang, Fanglin Zhang, Na Li, Yan Pan, Mengting Deng, Yue Wang, Jianping Li, Jiaxuan Qi. Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8. Cell cycle (Georgetown, Tex.). vol 19. issue 23. 2021-11-24. PMID:33198579. |
cell viability, and cell cycle and apoptosis were investigated by mtt assay and flow cytometry respectively. |
2021-11-24 |
2023-08-13 |
human |
| Yinghai Xie, Changwei Liu, Yinci Zhang, Amin Li, Chong Sun, Rui Li, Yingru Xing, Minghong Shi, Qi Wan. PKI-587 enhances radiosensitization of hepatocellular carcinoma by inhibiting the PI3K/AKT/mTOR pathways and DNA damage repair. PloS one. vol 16. issue 10. 2021-11-24. PMID:34665844. |
mtt, colony formation, flow cytometry, and immunofluorescence were used to analyze the proliferation, cell cycle, formation of residual γ-h2ax foci, and apoptosis of hcc cells. |
2021-11-24 |
2023-08-13 |
Not clear |
| Shanshan Song, Yang Yang, Jing He, Junfeng Tan. [miR-494 promotes islet β cell proliferation, inhibits apoptosis and increases insulin secretion by activating Wnt/β-catenin signaling pathway]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 37. issue 11. 2021-11-24. PMID:34809740. |
the activity of ins-1 cells was detected by mtt assay; apoptosis was detected by flow cytometry. |
2021-11-24 |
2023-08-13 |
human |