| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Dan Liu, Ye Cheng, Zhipeng Tang, Junliang Chen, Ying Xia, Chengbin Xu, Xiangyu Ca. Potential mechanisms of methylglyoxal-induced human embryonic kidney cells damage: Regulation of oxidative stress, DNA damage, and apoptosis. Chemistry & biodiversity. 2021-12-28. PMID:34962083. |
cell viability, apoptosis and the signaling pathways were measured with mtt, fluorescent staining and western blot experiments, the results showed that mgo could induce oxidative stress and cell inflammation, the level of reactive oxygen species (ros) increased, and p38mapk, jnk and nf-κb signaling pathways were activated. |
2021-12-28 |
2023-08-13 |
human |
| Yang Zhai, Bu-Gu Liu, Xue-Ni Mo, Min Zou, Xiao-Ping Mei, Wei Chen, Guo-Dong Huang, Lin W. Gingerol ameliorates neuronal damage induced by hypoxia-reoxygenation via the miR-210/brain-derived neurotrophic factor axis. The Kaohsiung journal of medical sciences. 2021-12-28. PMID:34962339. |
the levels of viability, apoptosis, and proteins in n2a cells were determined using mtt assays, flow cytometry, and western blotting, respectively. |
2021-12-28 |
2023-08-13 |
Not clear |
| Yen-Po Chen, Po-Chang Shih, Chien-Wei Feng, Chang-Cheng Wu, Kuan-Hao Tsui, You-Hsien Lin, Hsiao-Mei Kuo, Zhi-Hong We. Pardaxin Activates Excessive Mitophagy and Mitochondria-Mediated Apoptosis in Human Ovarian Cancer by Inducing Reactive Oxygen Species. Antioxidants (Basel, Switzerland). vol 10. issue 12. 2021-12-24. PMID:34942985. |
pardaxin induced apoptosis as determined by mtt and tunel assays, as well as activation of caspases-9/3, bid, t-bid, and bax, whereas bcl-2 was downregulated. |
2021-12-24 |
2023-08-13 |
human |
| Jin Zou, Zi-Lin Wan, Lan Yao, Meng-Yao Xu, Shu-Rong Wan. [Rhein inhibits hydrogen peroxide-induced apoptosis of human umbilical vein endothelial cells and its mechanism]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 23. 2021-12-24. PMID:34951247. |
cell survival rate was detected by mtt assay, apoptosis by annexin v-fitc/pi double staining and hoechst 33258 fluorescence staining, autophagy by ad-mcherry-gfp-lc3 b adenovirus transfection, and protein expression by western blot. |
2021-12-24 |
2023-08-13 |
human |
| Xiaofeng Deng, Yongqing Liu, Zesheng Xu, Zichao Wan. lncRNA NRON knockdown alleviates hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis by upregulating HIF-1α expression. Journal of cardiovascular pharmacology. 2021-12-22. PMID:34935702. |
the viability and apoptosis of these cells were evaluated by mtt assay and flow cytometer, respectively. |
2021-12-22 |
2023-08-13 |
mouse |
| Shuang Liu, Wen Yan, Yanbing Hu, Huiying W. Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway. Evidence-based complementary and alternative medicine : eCAM. vol 2021. 2021-12-16. PMID:34912465. |
the mtt method was used to detect cell viability, flow cytometry was used to detect cell apoptosis and reactive oxygen species (ros) levels, and western blot was used to detect protein levels. |
2021-12-16 |
2023-08-13 |
human |
| Fang-Liang Huang, Sheng-Jie Yu, En-Chih Liao, Long-Yuan Li, Pei-Wen Shen, Chia-Ling L. Niclosamide suppresses T‑cell acute lymphoblastic leukemia growth through activation of apoptosis and autophagy. Oncology reports. vol 47. issue 2. 2021-12-16. PMID:34913075. |
following niclosamide treatment, t‑all cell viability was evaluated using mtt assay, and apoptosis with annexin v/propidium iodide staining. |
2021-12-16 |
2023-08-13 |
Not clear |
| Wangchao Dong, Shanshan Wang, Weiqing Qian, Suming Li, Peimin Wan. Cedrol alleviates the apoptosis and inflammatory response of IL-1β-treated chondrocytes by promoting miR-542-5p expression. In vitro cellular & developmental biology. Animal. 2021-12-11. PMID:34893958. |
cell viability and apoptosis were measured by mtt and flow cytometry assays, respectively. |
2021-12-11 |
2023-08-13 |
rat |
| Ying Yang, Zhifang Yang, Ruili Zhang, Chunli Jia, Rui Mao, Shaya Mahati, Yuefen Zhang, Ge Wu, Yan Na Sun, Xiao Yan Jia, Ainiwaer Aimudula, Hua Zhang, Yongxing Ba. MiR-27a-3p enhances the cisplatin sensitivity in hepatocellular carcinoma cells through inhibiting PI3K/Akt pathway. Bioscience reports. vol 41. issue 12. 2021-12-09. PMID:34096570. |
next, we conducted a series of in vitro assays, including mtt, apoptosis and cell cycle assays to observe the biological changes. |
2021-12-09 |
2023-08-13 |
Not clear |
| Ping-Ping Zhang, Feng Zhang, Kai Zhu, Jun-Feng Zhu, Yuan Yuan, Yan-Li Yang, Lin Liu, Meng Wang, Jia-Jia L. Matrine exerted an anti-tumor effect on acute myeloid leukemia via the lncRNA LINC01116/miR-592-mediated JAK/STAT pathway inactivation. Neoplasma. 2021-12-09. PMID:34881627. |
in vitro, cell viability, apoptosis, and productions of inflammatory cytokines including il-1β, il-6, and tnf-α were tested by mtt, annexin v-fitc/pi staining, and elisa, respectively. |
2021-12-09 |
2023-08-13 |
mouse |
| Chen Yan, Jiang Ying, Wang Lu, Yang Changzhi, Qian Qihong, Mao Jingzhu, Sun Dongjie, Zhu Tingtin. MiR-1294 suppresses ROS-dependent inflammatory response in atopic dermatitis via restraining STAT3/NF-κB pathway. Cellular immunology. vol 371. 2021-12-08. PMID:34784561. |
cell viability, cell cycle, and apoptosis were respectively calculated using a methylthiazolyldiphenyl-tetrazolium bromide (mtt) assay and flow cytometry. |
2021-12-08 |
2023-08-13 |
mouse |
| Zidong Wang, Bei Wang, Jian Zhang, Zhensong Wu, Liankui Yu, Zhongye Su. Chemokine (C-C Motif) Ligand 2/Chemokine Receptor 2 (CCR2) Axis Blockade to Delay Chondrocyte Hypertrophy as a Therapeutic Strategy for Osteoarthritis. Medical science monitor : international medical journal of experimental and clinical research. vol 27. 2021-12-08. PMID:34876548. |
moreover, the viability of chondrocytes was analyzed using an mtt assay and flow cytometry was used to assess cell apoptosis. |
2021-12-08 |
2023-08-13 |
Not clear |
| Xuan Zhang, Chunbao Chen, Bei Li, Wei L. Circ-UQCRC2 aggravates lipopolysaccharide-induced injury in human bronchial epithelioid cells via targeting miR-495-3p/MYD88-mediated inflammatory response and oxidative stress. Autoimmunity. vol 54. issue 8. 2021-12-07. PMID:34499003. |
cell viability, apoptosis, and inflammatory response were assessed by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometry, and enzyme-linked immunosorbent assay (elisa). |
2021-12-07 |
2023-08-13 |
human |
| Shi-Yao Bai, Meng-Lu Li, Yuan Ren, Xin-Ming S. HDAC8-inhibitor PCI-34051-induced exosomes inhibit human bronchial smooth muscle cell proliferation via miR-381-3p mediated TGFB3. Pulmonary pharmacology & therapeutics. vol 71. 2021-12-07. PMID:34740750. |
the mtt, flow cytometry and tunel assays were performed to reveal the effect of pci-34051 and pci-exo on the proliferation and apoptosis of hbsmcs. |
2021-12-07 |
2023-08-13 |
human |
| Jing Ke, Kan Ni, Huimin Xue, Jia L. RBM38 is negatively regulated by miR-320b and enhances Adriamycin resistance in breast cancer cells. Oncology letters. vol 23. issue 1. 2021-12-07. PMID:34868364. |
in addition, the results of the mtt assay revealed that the overexpression of rbm38 enhanced the resistance of mcf-7/a cells to adm, promoted invasiveness, as determined using a transwell assay, inhibited the apoptosis of resistant cells, as determined using flow cytometry, and accelerated cell cycle progression from the g |
2021-12-07 |
2023-08-13 |
Not clear |
| Wenbo Hao, Fei Lin, Hanbing Shi, Zhanjiang Guan, Yunfei Jian. Long non-coding RNA OIP5-AS1 regulates smoke-related chronic obstructive pulmonary disease via targeting micro RNA -410-3p/IL-13. Bioengineered. vol 12. issue 2. 2021-12-07. PMID:34872453. |
the viable cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and flow cytometry was used to detect the cell apoptosis. |
2021-12-07 |
2023-08-13 |
Not clear |
| Huanhuan Zhu, Shaopeng Tong, Yan Cui, Xiaodong Wang, Minying Wan. Tanshinol alleviates ulcerative colitis by promoting the expression of VLDLR. Drug development research. vol 82. issue 8. 2021-12-06. PMID:34145621. |
flow cytometry and mtt was used to identify the apoptosis and viability of fhc cells, respectively. |
2021-12-06 |
2023-08-13 |
mouse |
| Hossam Kamli, Evan P Owens, David A Vesey, Rajagopalan Prasanna, Li Li, Glenda C Gobe, Christudas Morai. Overcoming sunitinib resistance with tocilizumab in renal cell carcinoma: Discordance between in vitro and in vivo effects. Biochemical and biophysical research communications. vol 586. 2021-12-06. PMID:34826699. |
cell viability was studied by mtt assay, and apoptosis by morphology and apoptag. |
2021-12-06 |
2023-08-13 |
mouse |
| Paraskevi Yfanti, Athanassios Karkabounas, Anna Batistatou, Alexia Tsapinou, Eleni Leneti, Georgios Manos, Marilena E Lekk. Study of potent cytotoxic activity of Helleborus cyclophyllus Boiss against a human adenocarcinoma cell line. Cytotechnology. 2021-12-03. PMID:33034799. |
cell morphology was examined after appropriate staining, cytotoxic activity of the extract was determined by the mtt assay, the type of cell death was analyzed by flow cytometry, confirmation of apoptosis was evaluated with the analysis of caspase-3, parp1 by western blotting, while the chemical composition was assessed by liquid chromatography-tandem mass spectrometry (lc-ms/ms). |
2021-12-03 |
2023-08-13 |
human |
| Qi Gao, Yunhua Wu, Chaoxiang Lu, Wang Kai, Weike Xie, Qi Wang, Lei Wang, Shiqi Liu, Yongkang Pa. Knockdown of RREB1 inhibits cell proliferation via enhanced p16 expression in gastric cancer. Cell cycle (Georgetown, Tex.). vol 20. issue 23. 2021-12-03. PMID:34666611. |
then, we established rreb1 overexpression and knockdown cell lines via lentiviral transfection and detected cell proliferation by using mtt, colony-forming, cell cycle and apoptosis assays in vitro. |
2021-12-03 |
2023-08-13 |
mouse |