| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yanyan Hu, Li Li, Wenbin Yin, Lin Shen, Beian You, Haiqing Ga. Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel. Molecular medicine reports. vol 10. issue 4. 2015-05-22. PMID:25109283. |
an mtt assay was used to examine the cell survival, and reactive oxygen species (ros) levels and apoptosis were detected by flow cytometry. |
2015-05-22 |
2023-08-13 |
rat |
| Qiang Bu, Yue Fang, Yuan Cao, Qiaoyun Chen, Yangchen Li. Enforced expression of miR-101 enhances cisplatin sensitivity in human bladder cancer cells by modulating the cyclooxygenase-2 pathway. Molecular medicine reports. vol 10. issue 4. 2015-05-22. PMID:25109742. |
the effect of mir‑101 and small interfering rna (sirna) against cox‑2 on cell viability was evaluated using mtt assays, and apoptosis levels were determined using fluorescence‑activated cell sorting analysis of annexin v/propidium iodide‑stained cells. |
2015-05-22 |
2023-08-13 |
human |
| Dongliang Cao, Yun Sun, Ling Wang, Qianchuan He, Juecun Zheng, Fei Deng, Shanshan Deng, ShuChing Chang, XiaoPing Yu, Minhui Li, Yao Meng, Jiagui Jin, Fubing She. Alpha-momorcharin (α-MMC) exerts effective anti-human breast tumor activities but has a narrow therapeutic window in vivo. Fitoterapia. vol 100. 2015-05-20. PMID:25447153. |
the mtt assay, the apoptosis test, and the cell cycle inhibition of α-mmc in human breast cancer cells were performed. |
2015-05-20 |
2023-08-13 |
mouse |
| Chaoqian Xu, Yingying Hu, Liangyu Hou, Jin Ju, Xiaoguang Li, Ning Du, Xiaoxiang Guan, Zhenhong Liu, Tianze Zhang, Wei Qin, Nannan Shen, Muhammad U Bilal, Yanjie Lu, Yong Zhang, Hongli Sha. β-Blocker carvedilol protects cardiomyocytes against oxidative stress-induced apoptosis by up-regulating miR-133 expression. Journal of molecular and cellular cardiology. vol 75. 2015-05-19. PMID:25066695. |
in vitro, carvedilol protected cardiomyocytes from h2o2 induced apoptosis detected by tunel staining and mtt assays, and increased mir-133 expression in cardiomyocytes. |
2015-05-19 |
2023-08-13 |
rat |
| Fei Cao, Zhihui Zhan. [The inhibitory effects of dexamethasone on cisplatin induced apoptosis of human lung adenocarcinoma cell SPC-A1 and its molecular mechanism]. Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi. vol 31. issue 3. 2015-05-19. PMID:25219252. |
the results of mtt showed that spc-a1 cells had resistance to cddp-induced apoptosis with pre-cultured dex and the resistance intensity presented dex concentration-dependent. |
2015-05-19 |
2023-08-13 |
human |
| Yaqiong Zu, Zhiyong Yang, Songshan Tang, Ying Han, Jun M. Effects of P-glycoprotein and its inhibitors on apoptosis in K562 cells. Molecules (Basel, Switzerland). vol 19. issue 9. 2015-05-18. PMID:25157469. |
cytotoxicity and apoptosis induced by psc833 were evaluated through an mtt method and apoptosis rate, and cell cycle combined with caspase 3 activity, respectively. |
2015-05-18 |
2023-08-13 |
Not clear |
| Yong-Can Huang, Xiao-He Chen, Jia Wang, Xiu-Qun Li, Hui-Qi Xie, Li Tang, Li Den. [Effects of hypoxia on human placental decidua basalis-mesenchymal stem cells proliferation, apoptosis and VEGF expression.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 60. issue 6. 2015-05-16. PMID:19082435. |
the proliferation and apoptosis of pdb-mscs were detected by mtt and flow cytometric analysis at the time points of 6 h, 12 h, 24 h, 48 h, 72 h, and 96 h, respectively. |
2015-05-16 |
2023-08-12 |
human |
| Jian-Ping Zhao, Min Gao, Yong-Jun Ye, Wei-Hua Hu, Zhi-Gang Zhou, Hong-Ling H. [Regulation of rat airway smooth muscle cell proliferation by mitochondrial ATP-sensitive K(+) channel in asthmic rats.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 61. issue 1. 2015-05-16. PMID:19224056. |
the proliferation and apoptosis of rat asmcs were examined respectively by mtt colorimetric assay and cell cycle analysis. |
2015-05-16 |
2023-08-12 |
rat |
| Jian-Ping Zhao, Min Gao, Yong-Jun Ye, Wei-Hua Hu, Zhi-Gang Zhou, Hong-Ling H. [Regulation of rat airway smooth muscle cell proliferation by mitochondrial ATP-sensitive K(+) channel in asthmic rats.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 61. issue 1. 2015-05-16. PMID:19224056. |
(1) after exposure to diazoxide for 24 h, the r-123 fluorescence intensity, the ros level, nf-κb mrna expression and the mtt absorbance value (a value) in normal asmcs were significantly increased, and the apoptosis of rat asmcs was significantly decreased compared to the control group (p<0.05). |
2015-05-16 |
2023-08-12 |
rat |
| Jian-Ping Zhao, Min Gao, Yong-Jun Ye, Wei-Hua Hu, Zhi-Gang Zhou, Hong-Ling H. [Regulation of rat airway smooth muscle cell proliferation by mitochondrial ATP-sensitive K(+) channel in asthmic rats.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 61. issue 1. 2015-05-16. PMID:19224056. |
however, there was no significant changes in those indices after the normal asmcs had been exposed to 5-hd for 24 h. (2) in asthma and asthma + diazoxide groups, the r-123 fluorescence intensity, ros level and the mtt a value were markedly increased, and the apoptosis was markedly decreased compared to control group (p<0.05). |
2015-05-16 |
2023-08-12 |
rat |
| Jian-Ping Zhao, Min Gao, Yong-Jun Ye, Wei-Hua Hu, Zhi-Gang Zhou, Hong-Ling H. [Regulation of rat airway smooth muscle cell proliferation by mitochondrial ATP-sensitive K(+) channel in asthmic rats.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 61. issue 1. 2015-05-16. PMID:19224056. |
5-hd partly weakened the effect of asthma on the r-123 fluorescence intensity, ros level and the mtt a value and the apoptosis of rat asmcs (p<0.05). |
2015-05-16 |
2023-08-12 |
rat |
| Jian-Ping Zhao, Min Gao, Yong-Jun Ye, Wei-Hua Hu, Zhi-Gang Zhou, Hong-Ling H. [Regulation of rat airway smooth muscle cell proliferation by mitochondrial ATP-sensitive K(+) channel in asthmic rats.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 61. issue 1. 2015-05-16. PMID:19224056. |
nf-κb mrna expression was positively correlated with the mtt a value and negatively correlated with the apoptosis of rat asmcs. |
2015-05-16 |
2023-08-12 |
rat |
| Shu-Tong Yao, Cheng Miao, Qing-Hua Liu, Yan-Yan Li, Hua Tian, Yun-Yun Wang, Bian-Ying Ma, Yong-Qi Fang, Shu-Cun Qi. [Inhibitory effect of quercetin preconditioning on tunicamycin-induced apoptosis in macrophages and its mechanism]. Sheng li xue bao : [Acta physiologica Sinica]. vol 65. issue 1. 2015-05-16. PMID:23426513. |
raw264.7 cells were pretreated with different concentrations (20, 40, and 80 μmol/l) of que for 30 min and then treated with tm (5 mg/l) for 12 h. cell viability and apoptosis were determined using mtt assay and annexin v-fitc apoptosis detection kit, respectively. |
2015-05-16 |
2023-08-12 |
Not clear |
| Hong-Nan Huo, Kun-Peng Xie, Li-Meng Wang, Ying Jiang, Wei Zou, Ming-Jie Xi. [Relationship between the inhibitory effect of fraxetin on breast cancer and estrogen signaling pathway]. Sheng li xue bao : [Acta physiologica Sinica]. vol 65. issue 3. 2015-05-16. PMID:23788190. |
the article used mtt assay, flow cytometer analysis and western blot to detect the inhibition of fraxetin on mcf-7 cell cycle distribution and apoptosis, erα, cyclin d1 and bcl-2 expression levels, mapk and pi3k signaling pathway to investigate the mechanism of anti-breast cancer of fraxetin. |
2015-05-16 |
2023-08-12 |
Not clear |
| Yuan Liu, Rui Chen, Nan Zhang, Xianlong Ye, Yin Bai, Yuquan Wei, Guiping Ren, Deshan L. [Anti-tumor efficacy of P53 with 9R cell-penetrating peptides]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology. vol 29. issue 7. 2015-05-16. PMID:24195362. |
we detected the rate of cell growth inhibition and apoptosis by mtt and annexin-v-fitc/pi double stained method respectively for measuring its effect on tumor cells. |
2015-05-16 |
2023-08-12 |
Not clear |
| Parisa Ghanbari, Mahsa Mohseni, Maryam Tabasinezhad, Bahman Yousefi, Amir Ata Saei, Simin Sharifi, Mohammad Reza Rashidi, Nasser Samad. Inhibition of survivin restores the sensitivity of breast cancer cells to docetaxel and vinblastine. Applied biochemistry and biotechnology. vol 174. issue 2. 2015-05-15. PMID:25086926. |
mtt assay and dapi staining were applied to study the anti-proliferative activity and apoptosis response of the agents. |
2015-05-15 |
2023-08-13 |
Not clear |
| Elisa Dieci, Lavinia Casati, Francesca Pagani, Fabio Celotti, Valeria Sibili. Acylated and unacylated ghrelin protect MC3T3-E1 cells against tert-butyl hydroperoxide-induced oxidative injury: pharmacological characterization of ghrelin receptor and possible epigenetic involvement. Amino acids. vol 46. issue 7. 2015-05-14. PMID:24705647. |
pretreatment with ghrelin (10(-7)-10(-11)m) significantly increased viability and reduced apoptosis of mc3t3-e1 cells cultured with t-bhp (250 μm) for three hours at the low concentration of 10(-9)m as shown by mtt assay and hoechst-33258 staining. |
2015-05-14 |
2023-08-13 |
Not clear |
| Xiaoxia Wen, Jianjun Zhu, Lei Dong, Yao Che. The role of c2orf68 and PI3K/Akt/mTOR pathway in human colorectal cancer. Medical oncology (Northwood, London, England). vol 31. issue 8. 2015-05-14. PMID:25023051. |
cell proliferation was tested by mtt assay, and apoptosis and cell cycle were studied by flow cytometry. |
2015-05-14 |
2023-08-13 |
human |
| Wei Zhang, Jian-Long Yao, Shan-Chao Dong, Feng-Qiang Hou, He-Ping Sh. SLPI knockdown induced pancreatic ductal adenocarcinoma cells proliferation and invasion. Cancer cell international. vol 15. 2015-05-08. PMID:25954138. |
cell proliferation and apoptosis were also evaluated by mtt assay and flow cytometry (fcm). |
2015-05-08 |
2023-08-13 |
human |
| Ping Zhang, He-Sheng Luo, Ming Li, Shi-Yun Ta. Artesunate inhibits the growth and induces apoptosis of human gastric cancer cells by downregulating COX-2. OncoTargets and therapy. vol 8. 2015-05-06. PMID:25945055. |
the effects of artesunate on the growth and apoptosis of gastric cancer cells were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometric analysis of annexin v-fluorescein isothiocyanate/propidium iodide staining, rhodamine 123 staining, and western blot analysis. |
2015-05-06 |
2023-08-13 |
human |