| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Dan Yang, Mingjin Li, Na D. Effects of the circ_101238/miR-138-5p/CDK6 axis on proliferation and apoptosis keloid fibroblasts. Experimental and therapeutic medicine. vol 20. issue 3. 2020-09-28. PMID:32782509. |
the expression levels of the proliferation marker, cdk6 and apoptosis marker, caspase-3 were determined via rt-qpcr and western blot analyses. |
2020-09-28 |
2023-08-13 |
Not clear |
| Gui-Nan Shen, Cheng Wang, Ying-Hua Luo, Jia-Ru Wang, Rui Wang, Wan-Ting Xu, Yi Zhang, Yu Zhang, Dong-Jie Zhang, Cheng-Hao Ji. 2-(6-Hydroxyhexylthio)-5,8-dimethoxy-1,4-naphthoquinone Induces Apoptosis through ROS-Mediated MAPK, STAT3, and NF- Evidence-based complementary and alternative medicine : eCAM. vol 2020. 2020-09-28. PMID:32849902. |
fluorescence microscopy and flow cytometry results indicated that hhdmnq could induce a549 cell apoptosis, and western blot analysis showed that hhdmnq induced apoptosis through regulating the mitochondria pathway, as well as the mapk, stat3, and nf- |
2020-09-28 |
2023-08-13 |
Not clear |
| Huijuan Wu, Hongmian Zhao, Li Che. Deoxyshikonin Inhibits Viability and Glycolysis by Suppressing the Akt/mTOR Pathway in Acute Myeloid Leukemia Cells. Frontiers in oncology. vol 10. 2020-09-28. PMID:32850379. |
the effects of deoxyshikonin on viability, apoptosis, caspase-3/7 activity, and cytochrome (cyt) c expression were evaluated by cell counting kit-8 assay, flow cytometry analysis, caspase-3/7 activity assay, and western blot analysis, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
| Aixin Zhao, Yunfang Qi, Kun Li. CLDN3 expression and function in pregnancy-induced hypertension. Experimental and therapeutic medicine. vol 20. issue 4. 2020-09-28. PMID:32855729. |
cell counting kit-8 (cck-8) assay, flow cytometry, transwell chamber assays, confocal laser scanning microscopy and western blot analysis were performed to detect cell proliferation, invasion, migration and apoptosis, in addition to matrix metalloproteinase (mmp) expression and erk1/2 phosphorylation. |
2020-09-28 |
2023-08-13 |
human |
| Arun Pandian Chandrasekaran, Naresh Poondla, Na Re Ko, Seung Jun Oh, Suresh Ramakrishn. YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation. Oncology letters. vol 20. issue 4. 2020-09-28. PMID:32863905. |
the mrna and protein levels of casp8- and fadd-like apoptosis regulator (cflip), death receptor 5 (dr5) and survivin were monitored using rt-pcr and western blot analysis, respectively. |
2020-09-28 |
2023-08-13 |
human |
| Guofu Yi, He Li, You Li, Fen Zhao, Zhiwei Ying, Menglan Liu, Jian Zhang, Xinqi Li. The protective effect of soybean protein-derived peptides on apoptosis via the activation of PI3K-AKT and inhibition on apoptosis pathway. Food science & nutrition. vol 8. issue 8. 2020-09-28. PMID:32884739. |
to further study its molecular mechanisms, western blot analysis demonstrated that sbp could activate the pi3k-akt pathway by increasing the phosphorylation of pi3k and akt and inhibiting apoptosis pathway by downregulating the expressions of pro-apoptotic proteins of bim, bax, fas, and fasl and promoting the expressions of anti-apoptotic proteins of bcl-xl and bcl-2. |
2020-09-28 |
2023-08-13 |
human |
| Weimin Yi, Xue Li, Ke Chen, Jianjun Li, Kefang Chen, Aizhen Pa. Effect of rNA interference on Oatp3a1 gene expression on biological characteristics and immune factors of ovarian granulosa cells in rats with PCOS. American journal of translational research. vol 12. issue 8. 2020-09-28. PMID:32913539. |
western blot, qrt-pcr, cck8 and flow cytometry were used to detect the gene expression, immune factor protein expression, cell proliferation and apoptosis in ovarian granulosa cells transfected with sirna oatp3a1 in rats with pcos, respectively. |
2020-09-28 |
2023-08-13 |
rat |
| Naoko Adachi, Ippei Kanazawa, Ken-Ichiro Tanaka, Ayumu Takeno, Masakazu Notsu, Sayuri Tanaka, Toshitsugu Sugimot. Insulin-Like Growth Factor-I Protects Against the Detrimental Effects of Advanced Glycation End Products and High Glucose in Myoblastic C2C12 Cells. Calcified tissue international. vol 105. issue 1. 2020-09-24. PMID:30809689. |
real-time pcr and western blot were performed to investigate mrna and protein expressions, and apoptosis was examined by using a dna fragment detection elisa kit. |
2020-09-24 |
2023-08-13 |
mouse |
| Pingan Wu, Xiaoxia Shi, Mengxin Luo, Inam-U-Llah, Kaixin Li, Mengren Zhang, Jingran Ma, Yuan Li, Yanqing Liu, Cong Zhang, Xiaofang Liu, Shuangyue Li, Qiujuan Li, Xiaochi Chen, Xiangyu Che, Fengyuan Pia. Taurine inhibits neuron apoptosis in hippocampus of diabetic rats and high glucose exposed HT-22 cells via the NGF-Akt/Bad pathway. Amino acids. vol 52. issue 1. 2020-09-24. PMID:31875259. |
morris water maze test was used to check the learning and memory ability, tunel assay was used to measure apoptosis and nerve growth factor (ngf); akt/bad pathway relevant protein was detected by western blot. |
2020-09-24 |
2023-08-13 |
rat |
| Li-Mei Zhang, Li-Li Jia, Wen-Li Y. [Serum exosomes of rats with hepatic ischemia-reperfusion injury induce the damage of hippocampus and cerebral cortex]. Sheng li xue bao : [Acta physiologica Sinica]. vol 72. issue 4. 2020-09-24. PMID:32820307. |
the morphology and size of exosomes were observed by transmission electron microscope, the expression of exosomes markers cd63 and cd9 was detected by western blot, and the damage of liver and brain, levels of apoptosis and oxidative stress response in hippocampus and cerebral cortex were observed by serological and histological indexes. |
2020-09-24 |
2023-08-13 |
rat |
| Bo Zhang, Haijun Zhang, Hao Luo, Chao Yang, Yi Yua. Sinomenine can promote the proliferation and differentiation of osteoblasts by regulating the Akt/Runx2 signaling pathway in MC3T3-E1 cells. Die Pharmazie. vol 74. issue 12. 2020-09-23. PMID:31907116. |
the proliferation and apoptosis of the cells were determined by mtt assay at 0, 24, 48 and 72 h, and flow cytometry method was used to determine the effect of sinomenine on the apoptosis of mc3t3-e1 cells at 72 h; furthermore, after 72 h, the cell culture supernatants were collected, and the levels of alkaline phosphatase and osteocalcin were examined by enzyme-linked immunosorbent assay (elisa); next, cells were collected, and the expression of type i collagen (col1a1), osteopontin (opn), protein kinase b (akt), runt-related transcription factor 2 (runx2), b-cell lymphoma 2 (bcl-2) and bcl-2-associated x protein (bax) were examined by rt-qpcr and western blot methods. |
2020-09-23 |
2023-08-13 |
mouse |
| Lina Zhao, Meili Zhai, Xu Yang, Hongjie Guo, Ying Cao, Donghui Wang, Ping Li, Chong Li. Dexmedetomidine attenuates neuronal injury after spinal cord ischaemia-reperfusion injury by targeting the CNPY2-endoplasmic reticulum stress signalling. Journal of cellular and molecular medicine. vol 23. issue 12. 2020-09-22. PMID:31625681. |
to determine whether the endoplasmic reticulum apoptosis pathway cnpy2-perk was involved in the neuroprotective mechanism of dex, the expression levels of related proteins (cnpy2, grp78, perk, chop, caspase-12, caspase-9 and caspase-3) were detected by western blot analysis and rt-pcr. |
2020-09-22 |
2023-08-13 |
rat |
| Ning Zhu, Yijia Xiang, Xuyong Zhao, Changhong Cai, Hao Chen, Wenbing Jiang, Yi Wang, Chunlai Zen. Thymoquinone suppresses platelet-derived growth factor-BB-induced vascular smooth muscle cell proliferation, migration and neointimal formation. Journal of cellular and molecular medicine. vol 23. issue 12. 2020-09-22. PMID:31638340. |
molecules including the mitochondria-dependent apoptosis factors, matrix metalloproteinase 2 (mmp2), matrix metalloproteinase 9 (mmp9), pten/akt and mitogen-activated protein kinases (mapks) were determined by western blot. |
2020-09-22 |
2023-08-13 |
rat |
| Md Shiblur Rahaman, Mahmuda Akter, Md Mostafizur Rahman, Md Tajuddin Sikder, Toshiyuki Hosokawa, Takeshi Saito, Masaaki Kurasak. Investigating the protective actions of D-pinitol against arsenic-induced toxicity in PC12 cells and the underlying mechanism. Environmental toxicology and pharmacology. vol 74. 2020-09-22. PMID:31786496. |
protein expression of western blot analysis showed that co-exposure of d-pinitol and arsenic significantly inhibited arsenic-induced autophagy which further suppressed apoptosis through up-regulation of survival factors; mtor, p-mtor, akt, p-akt, nf-кb, nrf2, erk1, gr, bcl-x and down-regulation of death factors; p53, bax, cytochrome c, lc3, although arsenic regulated those factors negatively. |
2020-09-22 |
2023-08-13 |
human |
| Weijie Yang, Ping Su. Downregulation of microRNA-129-5p increases the risk of intervertebral disc degeneration by promoting the apoptosis of nucleus pulposus cells via targeting BMP2. Journal of cellular biochemistry. vol 120. issue 12. 2020-09-18. PMID:31436339. |
in addition, western blot and real-time pcr were used to confirm the regulatory relationship between mir-129-5p and its targets, while flow cytometry was used to evaluate the effect of mir-129-5p on the apoptosis of neural progenitor cells (npcs). |
2020-09-18 |
2023-08-13 |
Not clear |
| Jiliang Yan, Dongya Zhang, Yanjie Han, Zhongjie Wang, Chunyan M. Antitumor activity of SR splicing-factor 5 knockdown by downregulating pyruvate kinase M2 in non-small cell lung cancer cells. Journal of cellular biochemistry. vol 120. issue 10. 2020-09-17. PMID:31106485. |
apoptosis was assessed by flow cytometry and western blot analysis of apoptosis-associated proteins including b-cell lymphoma 2 (bcl-2), bax, and cytochrome c (cyt c). |
2020-09-17 |
2023-08-13 |
Not clear |
| Gao-Jie Xin, Jian-Hua Fu, Xiao Han, Lei Li, Hao Guo, Hong-Xu Meng, Yu-Wei Zhao, Fei-Fan Jia, Jian-Xun Li. [Salvianolic acid B regulates mitochondrial autophagy mediated by NIX to protect H9c2 cardiomyocytes from hypoxia/reoxygenation injury]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 45. issue 12. 2020-09-17. PMID:32627473. |
the autophagy related proteins lc3-Ⅰ, lc3-Ⅱ, apoptosis related protein cleaved caspase-3 and mitochondrial autophagy receptor protein nix were detected by western blot. |
2020-09-17 |
2023-08-13 |
Not clear |
| Ying-Ying Tian, Ai-Lin Yang, Xiao-Nan Chen, Hao-Miao Ren, Ya-Xin Liu, Hai-Ling Qiu, Lei-Meng-Yuan Tang, Hui-Ming Huang, Peng-Fei Tu, Zhong-Dong H. [Effect of Huaier aqueous extract on growth and metastasis of human non-small cell lung cancer NCI-H1299 cells and its underlying mechanisms]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 45. issue 15. 2020-09-17. PMID:32893561. |
western blot was used to detect the levels of proteins involving apoptosis, epithelial-mesenchymal transition(emt), and mapk signaling pathway in nci-h1299 cells exposed to huaier aqueous extract. |
2020-09-17 |
2023-08-13 |
human |
| Li-Ping Wang, Yan-Qi Liu, Jie Cui, Li W. [Study on mechanism of curcumin in treatment of acute pancreatitis based on regulation of PI3K-Akt signaling pathway by miR-198]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 45. issue 15. 2020-09-17. PMID:32893562. |
the apoptosis was detected by tunel staining; the levels of serum amylase, lipase, bcl-2 and bax were detected by elisa, and the levels of pi3 k, akt and p-akt in pancreatic tissue were measured by western blot. |
2020-09-17 |
2023-08-13 |
rat |
| Li-Ping Wang, Yan-Qi Liu, Jie Cui, Li W. [Study on mechanism of curcumin in treatment of acute pancreatitis based on regulation of PI3K-Akt signaling pathway by miR-198]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 45. issue 15. 2020-09-17. PMID:32893562. |
western blot results showed that the expression levels of pi3 k and p-akt in pancreatic tissue of model rats were up-regulated after the intervention of curcumin, and the apoptosis rate of pancreatic cells decreased in tunel staining. |
2020-09-17 |
2023-08-13 |
rat |