| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Min Lu, Xinglei Qin, Jungong Yao, Yuanyuan Yang, Minghu Zhao, Lin Su. MiR-134-5p targeting XIAP modulates oxidative stress and apoptosis in cardiomyocytes under hypoxia/reperfusion-induced injury. IUBMB life. vol 72. issue 10. 2021-04-30. PMID:32797709. |
oxidative stress and cell apoptosis were determined by enzyme-linked immunosorbent assays, 3-(4, 5-dimethylthiazole-2-y1)-2, 5-biphenyl tetrazolium bromide assay, flow cytometry, western blot, and terminal-deoxynucleoitidyl transferase-mediated nick end labeling (tunel). |
2021-04-30 |
2023-08-13 |
Not clear |
| Changwei Zou, Hong Yang, Lanyue Cui, Xinyi Cao, Hong Huang, Tingtao Che. Potential hazardous effects of printing room PM2.5 exposure include promotion of lung inflammation and subsequent injury. Molecular medicine reports. vol 22. issue 4. 2021-04-30. PMID:32945461. |
western blot analysis indicated that pm2.5 significantly enhanced expression of phosphorylated (p)‑erk relative to total erk, cyclooxygenase‑2, p‑anti‑nuclear‑factor‑κb (p‑nf‑κb) relative to nf‑κb, transforming growth factor‑β1 and bax relative to bcl‑2 in inflammation (p<0.05), fibrosis and apoptosis signaling pathways. |
2021-04-30 |
2023-08-13 |
mouse |
| Maryam Mohammadlou, Maryam Abdollahi, Maral Hemati, Rasoul Baharlou, Ehsan Manouchehri Doulabi, Mehrnoosh Pashaei, Farahnaz Ghahremanfard, Mohammad Faranoush, Parviz Kokhae. Apoptotic effect of berberine via Bcl-2, ROR1, and mir-21 in patients with B-chronic lymphocytic leukemia. Phytotherapy research : PTR. vol 35. issue 4. 2021-04-30. PMID:33174291. |
although western blot results did not show any change in bax as a pro-apoptotic protein, an increased bax/bcl-2 ratio indicated that mitochondrial pathway is involved in berberine-induced apoptosis of cll cells. |
2021-04-30 |
2023-08-13 |
Not clear |
| Sang-Eun Jung, Jin Seop Ahn, Yong-Hee Kim, Seok-Man Kim, Tea Gun Um, Bang-Jin Kim, Buom-Yong Ry. Inhibition of Caspase-8 Activity Improves Freezing Efficiency of Male Germline Stem Cells in Mice. Biopreservation and biobanking. 2021-04-30. PMID:33926212. |
the activities of caspase-8, caspase-9, caspase-3, and caspase-7 were assessed by western blot analyses to determine the role of specific apoptosis pathways in gsc cryoinjury. |
2021-04-30 |
2023-08-13 |
mouse |
| Jie Li, Qiang Li, He Huang, Yinguang Li, Li Li, Wenhui Hou, Zeshan Yo. Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer. International journal of oncology. vol 50. issue 4. 2021-04-27. PMID:28350128. |
in the present study, the expression levels of mir-221 and the apoptosis protease activating factor 1 (apaf1) protein in 63 samples of ovarian cancer tissues and the cell lines, iose25, a2780, ovcar3, skov3 and 3ao were detected by quantitative reverse-transcription polymerase chain reaction (qrt-pcr) and western blot analysis, respectively. |
2021-04-27 |
2023-08-13 |
human |
| Jiong Zhang, Xiaojun Den. Effects of miR-599 targeting YAP1 on proliferation, invasion and apoptosis of bladder urothelial carcinoma cells. Experimental and molecular pathology. vol 118. 2021-04-27. PMID:33359177. |
j82 cells cultured in vitro were divided into mir-nc group (transfected with analog negative control mir-nc), mir-599 group (transfected with mir-599 analog), mir-599 + pcdna3.1 group (co transfected with mir-599 analog and pcdna3.1 empty vector), mir-599 + pcdna3.1-yap1 group (co transfected with mir-599 analog and pcdna3.1-yap1 over expression vector), the targeting relationship between mir-599 and yap1 was detected by double luciferase reporter gene assay, the expression level of mir-599 was detected by real-time fluorescence quantitative pcr, the expression of yap1 was detected by western blot, cell proliferation was detected by mtt method, cell invasion was detected by transwell's cell experiment, and apoptosis was detected by flow cytometry. |
2021-04-27 |
2023-08-13 |
Not clear |
| Yan Wang, Qin Niu, Jie Dai, Haitang Shi, Jinzhi Zhan. circCUX1 promotes neuroblastoma progression and glycolysis by regulating the miR-338-3p/PHF20 axis. General physiology and biophysics. vol 40. issue 1. 2021-04-27. PMID:33655888. |
cell proliferation and apoptosis were evaluated by colony formation assay, flow cytometry and western blot analysis. |
2021-04-27 |
2023-08-13 |
Not clear |
| Yujie Zhao, Wenyan Guo, Xiaolian Gu, Chao Chang, Jine W. Repression of deoxynivalenol-triggered cytotoxicity and apoptosis by mannan/β-glucans from yeast cell wall: Involvement of autophagy and PI3K-AKT-mTOR signaling pathway. International journal of biological macromolecules. vol 164. 2021-04-23. PMID:32735928. |
we analyzed the cell morphology, cell activity, oxidative stress, fluorescence intensity and expressions of proteins relevant to autophagy, apoptosis and pi3k-akt-mtor signaling pathway by using inverted microscopy, mts, reactive oxygen species (ros), glutathione (gsh) and malondialdehyde (mda) assay, annexin v-fitc / propidium iodide (pi) double staining and western blot assay. |
2021-04-23 |
2023-08-13 |
Not clear |
| Shuxia Guo, Juanjuan Sun, Yan Zhuan. Quercetin alleviates lipopolysaccharide-induced inflammatory responses by up-regulation miR-124 in human renal tubular epithelial cell line HK-2. BioFactors (Oxford, England). vol 46. issue 3. 2021-04-22. PMID:31804760. |
western blot was performed to examine the expression of apoptosis and inflammation-associated mediators. |
2021-04-22 |
2023-08-13 |
human |
| Yingjie Ding, Jing Wang, Hongliang Zhang, Huanting L. Long noncoding RNA-GAS5 attenuates progression of glioma by eliminating microRNA-10b and Sirtuin 1 in U251 and A172 cells. BioFactors (Oxford, England). vol 46. issue 3. 2021-04-22. PMID:31889362. |
we artificially modulated gas5 to explore its roles in viability assayed by cell counting kit-8 (cck-8), motile activities by 24-transwell assay, as well as apoptosis by a flow cytometer and western blot assay. |
2021-04-22 |
2023-08-13 |
Not clear |
| D-L Cai, L-X Chen, L-M Wang, T-N Zha. Effects of homeodomain-interacting protein kinase 2 on renal tubular epithelial cells. European review for medical and pharmacological sciences. vol 24. issue 11. 2021-04-21. PMID:32572889. |
to research the role of homeodomain-interacting protein kinase 2 (hipk2) on the oxidative stress (os) and apoptosis induced by hypoxia/reoxygenation (h/r) of normal rat kidney-52e (nrk-52e) cells, through the jak2/stat3 signaling pathway materials and methods: first, we transfected the nrk-52e cells with small interfering rna (sirna) of hipk2 by lipofectaminetm 2000, real time-polymerase chain reaction (rt-pcr) and western blot (wb) were used to test the efficiency of transfection after 48 h. after cells were treated with h/r, we tested cell apoptosis by cell counting kit-8 (cck-8) assay, flow cytometry, tunel staining, pcr, and western blot. |
2021-04-21 |
2023-08-13 |
rat |
| Qiuyan Zhao, Changfu Hao, Jingjing Wei, Ruoxuan Huang, Chao Li, Wu Ya. Bone marrow-derived mesenchymal stem cells attenuate silica-induced pulmonary fibrosis by inhibiting apoptosis and pyroptosis but not autophagy in rats. Ecotoxicology and environmental safety. vol 216. 2021-04-21. PMID:33848736. |
we detected the pathological and fibrotic changes, apoptosis, autophagy, and pyroptosis in their lung tissue by histopathological examination, hydroxyproline content assays, real-time quantitative polymerase chain reactions, western blot assays, immunohistochemistry staining, immunofluorescence staining, and enzyme-linked immunosorbent assays. |
2021-04-21 |
2023-08-13 |
rat |
| Xinna Du, Xuan Shen, Li Dai, Fangjie Bi, Hu Zhang, Chunfeng L. PSMD12 promotes breast cancer growth via inhibiting the expression of pro-apoptotic genes. Biochemical and biophysical research communications. vol 526. issue 2. 2021-04-20. PMID:32222279. |
flow cytometry showed that cell apoptosis increased in the psmd12 knockdown, and rna-seq indicated that the apoptosis pathway was activated, and the txnip, gadd45a, gadd45b, rhob, and cdkn1a pro-apoptotic genes were highly expressed, a result that was validated by rt-qpcr and western blot. |
2021-04-20 |
2023-08-13 |
mouse |
| Shuangyuan Lei, Suli Wu, Guanzhuo Wang, Bing Li, Bin Liu, Xia Le. Pinoresinol diglucoside attenuates neuroinflammation, apoptosis and oxidative stress in a mice model with Alzheimer's disease. Neuroreport. vol 32. issue 3. 2021-04-19. PMID:33470758. |
western blot assay results have shown that pdg could also upregulate the ratio of bcl-2/bax and downregulate cytochrome c and cleaved caspase-3 expressions, thereby inhibiting neuronal apoptosis. |
2021-04-19 |
2023-08-13 |
mouse |
| Zheng Wei, Tingting Li, Yanping Sun, Huilin Su, Yuanning Zeng, Qiuhong Wang, Haixue Kuan. Daturataturin A, a withanolide in Datura metel L., induces HaCaT autophagy through the PI3K-Akt-mTOR signaling pathway. Phytotherapy research : PTR. vol 35. issue 3. 2021-04-19. PMID:33560581. |
the pharmacodynamics and mechanism of dta were studied by western blot, immunofluorescence, apoptosis and proliferation detection, and real-time quantitative polymerase chain reaction. |
2021-04-19 |
2023-08-13 |
human |
| Ya-Jun Zhao, Jun Zhang, Yong-Cang Wang, Liang Wang, Xin-Yang H. MiR-450a-5p Inhibits Gastric Cancer Cell Proliferation, Migration, and Invasion and Promotes Apoptosis Frontiers in oncology. vol 11. 2021-04-16. PMID:33854971. |
we used gain-of-function approaches to investigate the role of mir-450a-5p on gastric cancer cell proliferation, migration, invasion, and apoptosis using biological and molecular techniques including real-time quantitative pcr (rt-qpcr), cck-8, colony formation, flow cytometry, western blot, wound healing, transwell chamber, dual luciferase reporter, and tumor xenograft mouse model. |
2021-04-16 |
2023-08-13 |
mouse |
| Qijun Si, Yujie Shi, Dandan Huang, Na Zhan. Diosmetin alleviates hypoxia‑induced myocardial apoptosis by inducing autophagy through AMPK activation. Molecular medicine reports. vol 22. issue 2. 2021-04-15. PMID:32627001. |
cell viability was determined using cell counting kit‑8, cell apoptosis was determined by annexin v‑fitc apoptosis detection kit and cleaved caspase‑3 level was assessed by western blot analysis. |
2021-04-15 |
2023-08-13 |
Not clear |
| Liu Huang, Bingyan Guo, Suyun Liu, Chenglong Miao, Yongjun L. Inhibition of the LncRNA Gpr19 attenuates ischemia-reperfusion injury after acute myocardial infarction by inhibiting apoptosis and oxidative stress via the miR-324-5p/Mtfr1 axis. IUBMB life. vol 72. issue 3. 2021-04-14. PMID:31622017. |
a tunel assay, western blot, and oxidative stress analysis were conducted in this study to determine apoptosis and oxidative stress levels. |
2021-04-14 |
2023-08-13 |
mouse |
| Zhiqiao Xu, Xiaojing Tie, Ning Li, Zhenying Yi, Fengqian Shen, Yan Zhan. Circular RNA hsa_circ_0000654 promotes esophageal squamous cell carcinoma progression by regulating the miR-149-5p/IL-6/STAT3 pathway. IUBMB life. vol 72. issue 3. 2021-04-14. PMID:31778020. |
furthermore, interleukin 6 (il-6) and signal transducer and activator of transcription 3 (stat3) expression in cells was detected by rt-pcr and western blot analysis, while cck8, brdu, flow cytometry, and transwell assays were used to monitor cell proliferation, apoptosis, migration and invasion, respectively. |
2021-04-14 |
2023-08-13 |
mouse |
| Jianwen Long, Xianming P. Polyphyllin I Promoted Melanoma Cells Autophagy and Apoptosis via PI3K/Akt/mTOR Signaling Pathway. BioMed research international. vol 2020. 2021-04-13. PMID:32733943. |
cck-8 assay was utilized to detect cell proliferation; cell apoptosis and cell cycle were measured by flow cytometry; western blot was used to examine the expressions of proteins. |
2021-04-13 |
2023-08-13 |
mouse |