| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jing Cui, Hao Li, Ying Wang, Tian Tian, Chao Liu, Yanan Wang, Shukai Sun, Baisui Fen. Skullcapflavone I has a potent anti-pancreatic cancer activity by targeting miR-23a. BioFactors (Oxford, England). vol 46. issue 5. 2021-07-29. PMID:32141657. |
cell viability, bromodeoxyuridine (brdu)-positive cell rate, apoptosis, migration, invasion, and related protein expression were assessed by utilizing cell counting kit-8 (cck-8), brdu staining, apoptosis assessment, transwell assay, and western blot. |
2021-07-29 |
2023-08-13 |
human |
| Jialiang Yang, Yixuan Li, Lili Wang, Zhenchun Zhang, Zunzhong Li, Qian Ji. LncRNA H19 aggravates TNF-α-induced inflammatory injury via TAK1 pathway in MH7A cells. BioFactors (Oxford, England). vol 46. issue 5. 2021-07-29. PMID:32525617. |
the influences of tumor necrosis factor-α (tnf-α) on cell viability, apoptosis, and inflammatory factor expression were, respectively, detected through cell counting kit-8 (cck-8), flow cytometry, quantitative reverse transcription polymerase chain reaction (qrt-pcr), enzyme-linked immunosorbent assay (elisa) assay and western blot. |
2021-07-29 |
2023-08-13 |
Not clear |
| Shizhang Liu, Keke Duan, Xiaoxia Zhang, Xiane Cao, Xixia Wang, Fanbin Meng, Huitong Liu, Bingqiang Xu, Xi Wan. Circ_0081001 down-regulates miR-494-3p to enhance BACH1 expression and promotes osteosarcoma progression. Aging. vol 13. issue 13. 2021-07-29. PMID:34191748. |
cell counting kit-8 (cck-8) assay, together with 5-ethynyl-2'-deoxyuridine (edu) assay, was performed for evaluating cell proliferation; the alterations in apoptosis were analyzed utilizing flow cytometry; transwell assay was conducted for examining cell migration and invasion; moreover, western blot was utilized for the quantification of bach1 protein expression; bioinformatics, dual-luciferase reporter gene, and rna-binding protein immunoprecipitation assays were executed for validating the binding relationships between circ_0081001 and mir-494-3p, and between mir-494-3p and bach1. |
2021-07-29 |
2023-08-13 |
Not clear |
| Xiu-Ling Liang, Yu-Long Wang, Pei-Rong Wan. MiR-200a with CDC7 as a direct target declines cell viability and promotes cell apoptosis in Wilm's tumor via Wnt/β-catenin signaling pathway. Molecular and cellular biochemistry. vol 476. issue 6. 2021-07-28. PMID:33599894. |
also, western blot was applied to measure the expression of cdc7 as well as wnt/β-catenin signaling pathway-related proteins and apoptosis proteins. |
2021-07-28 |
2023-08-13 |
Not clear |
| Bai-Ling Sun, En-Bo Cai, Yan Zhao, Yu Wang, Li-Min Yang, Jing-Yao Wan. Arctigenin Triggers Apoptosis and Autophagy via PI3K/Akt/mTOR Inhibition in PC-3M Cells. Chemical & pharmaceutical bulletin. vol 69. issue 5. 2021-07-27. PMID:33627540. |
in addition, the expression levels of apoptosis and autophagy-related proteins were analyzed by western blot. |
2021-07-27 |
2023-08-13 |
Not clear |
| Lingfan Xiong, Wenhao Guo, Yong Yang, Danping Gao, Jun Wang, Yuanyuan Qu, Ying Zhan. Tectoridin inhibits the progression of colon cancer through downregulating PKC/p38 MAPK pathway. Molecular and cellular biochemistry. vol 476. issue 7. 2021-07-27. PMID:33683556. |
flow cytometry and western blot were conducted to examine apoptosis. |
2021-07-27 |
2023-08-13 |
mouse |
| Hui Wang, Arunachalam Chinnathambi, Tahani Awad Alahmadi, Sulaiman Ali Alharbi, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan, Sardar Hussain, Kavitha Ramamoorthy, Thamaraiselvan Rengaraja. Phyllanthin inhibits MOLT-4 leukemic cancer cell growth and induces apoptosis through the inhibition of AKT and JNK signaling pathway. Journal of biochemical and molecular toxicology. vol 35. issue 6. 2021-07-27. PMID:33724660. |
the level of reactive oxygen species, the membrane potential of mitochondria, apoptosis by 2',7'-dichlorofluorescin-diacetate (dcf-da), rhodamine, acridine orange (ao)/ethidium bromide (eb), 4',6-diamidino-2-phenylindole (dapi)/propidium iodide (pi) staining, gene expression of signaling molecules, and protein levels were assessed by reverse-transcription polymerase chain reaction and western blot analysis. |
2021-07-27 |
2023-08-13 |
Not clear |
| Xueying Chen, Xiaoying He, Yan Guo, Liehua Liu, Hai Li, Jinfu Tan, Weidong Feng, Hongyu Guan, Xiaopei Cao, Haipeng Xiao, Yanbing L. Glucose-dependent insulinotropic polypeptide modifies adipose plasticity and promotes beige adipogenesis of human omental adipose-derived stem cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 35. issue 5. 2021-07-27. PMID:33817830. |
annexin v ftic/pi double stain, tunel staining, and western blot were applied for apoptosis evaluation. |
2021-07-27 |
2023-08-13 |
human |
| Bocong Ma, Ming Zhao, Zhihao Gu. Circular RNA circ_0010729 Knockdown Attenuates Oxygen-Glucose Deprivation-Induced Human Cardiac Myocytes Injury by miR-338-3p/CALM2 Axis. Journal of cardiovascular pharmacology. vol 77. issue 5. 2021-07-27. PMID:33951696. |
ogd-induced damage in ac16 cells was assessed by cell viability, apoptosis, and autophagy using cell counting kit-8, flow cytometry, and western blot analyses. |
2021-07-27 |
2023-08-13 |
human |
| K E Lines, P Filippakopoulos, M Stevenson, S Müller, H E Lockstone, B Wright, S Knapp, D Buck, C Bountra, R V Thakke. Effects of epigenetic pathway inhibitors on corticotroph tumour AtT20 cells. Endocrine-related cancer. vol 27. issue 3. 2021-07-26. PMID:31935194. |
rna-sequence analysis, which was confirmed by qrt-pcr and western blot analyses, revealed that jq1 treatment significantly altered expression of genes involved in apoptosis, such as nfκb, and the somatostatin receptor 2 (sstr2) anti-proliferative signalling pathway, including sstr2. |
2021-07-26 |
2023-08-13 |
Not clear |
| Yan Sun, Juening Kang, Xiaofeng Guan, Hua Xu, Xiang Wang, Yaoliang Den. Regulation of endoplasmic reticulum stress on the damage and apoptosis of renal tubular epithelial cells induced by calcium oxalate crystals. Urolithiasis. vol 49. issue 4. 2021-07-26. PMID:33786645. |
western blot and tunel staining were used to detect cell apoptosis. |
2021-07-26 |
2023-08-13 |
human |
| Xiaoyuan Xu, Xinli Song, Xiaohua Xu, Yanluan Zheng, Lan Xu, Ling She. Inhibition of sestrin 1 alleviates polycystic ovary syndrome by decreasing autophagy. Aging. vol 13. issue 8. 2021-07-26. PMID:33883304. |
rt-qpcr, western blot, cell counting kit-8 (cck-8), edu (5-ethynyl-2'-deoxyuridine) staining, lc3 staining, ros (reactive oxygen species) detection, and apoptosis assays were conducted to explore the effects of sestrin 1 on kgn human granulosa-like tumor cells. |
2021-07-26 |
2023-08-13 |
human |
| Piman Pocasap, Apiyada Nonpunya, Natthida Weerapreeyaku. Pinus kesiya Royle ex Gordon induces apoptotic cell death in hepatocellular carcinoma HepG2 cell via intrinsic pathway by PARP and Topoisomerase I suppression. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 139. 2021-07-26. PMID:33940508. |
the apoptosis-inducing effect was determined based on caspases activity, mitochondria membrane potential change, and expression of proteins related to apoptosis by western blot. |
2021-07-26 |
2023-08-13 |
Not clear |
| Xiaoyong Sun, Bin Wu, Liguo Geng, Jinfeng Zhang, Guozheng Qi. Xiaokang Liuwei Dihuang decoction ameliorates the immune infertility of male rats induced by lipopolysaccharide through regulating the levels of sex hormones, reactive oxygen species, pro-apoptotic and immune factors. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 139. 2021-07-26. PMID:33951576. |
results of tunel staining, elisa assay and western blot showed that compared with lps group, the testicular cell apoptosis and the levels of interleukin 1β (il-1β), tumor necrosis factor α (tnf-α), asab, malondialdehyde (mda) and toll-like receptor 2 (tlr2) in the testicular tissue significantly decreased in three xiaokang liuwei dihuang decoction groups. |
2021-07-26 |
2023-08-13 |
rat |
| Peng Zhang, Xue Zhang, Jing Zhang, Yanqiu Song, Ting Liu, Zhican Zeng, Xiaofeng Fu, Han Fu, Hong Zhang, Qin Qin, Naikuan Fu, Zhigang Gu. Novel Nanoliposomes Alleviate Contrast-Induced Nephropathy by Mediating Apoptosis Response in New Zealand Rabbits. Frontiers in molecular biosciences. vol 8. 2021-07-24. PMID:34295921. |
compared with the hydration group, the nano liposome group showed improved protection of renal function, with significantly different scr and bun levels, incidence of cin, apoptosis index, rt-pcr and western blot protein expression patterns. |
2021-07-24 |
2023-08-13 |
Not clear |
| Han-You Wu, Xiang-Cheng Zhang, Bing-Bing Jia, Ye Cao, Kai Yan, Jing-Yuan Li, Li Tao, Zhi-Gang Jie, Quan-Wen Li. Exosomes derived from human umbilical cord mesenchymal stem cells alleviate acetaminophen-induced acute liver failure through activating ERK and IGF-1R/PI3K/AKT signaling pathway. Journal of pharmacological sciences. vol 147. issue 1. 2021-07-23. PMID:34294366. |
western blot showed that hucmsc-exo significantly promoted the activation of erk1/2 and igf-1r/pi3k/akt signaling pathways in apap-injured lo2 cells, resulting in the inhibition of apoptosis of lo2 cells. |
2021-07-23 |
2023-08-13 |
mouse |
| Toria Tomasek, Lorraine B Ware, Julie A Bastarache, Jamie E Meega. Cell-free hemoglobin-mediated human lung microvascular endothelial barrier dysfunction is not mediated by cell death. Biochemical and biophysical research communications. vol 556. 2021-07-20. PMID:33848934. |
while cfh caused a small but significant decrease in cell viability measured by the membrane impermeable dna dye draq7 in human lung microvascular endothelial cells, cfh did not increase apoptosis as measured by tunel staining or western blot for cleaved caspase-3. |
2021-07-20 |
2023-08-13 |
mouse |
| Wenwen Du, Li Wang, Zhen Liao, Juan Wan. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation. vol 44. issue 4. 2021-07-19. PMID:33710445. |
cell viability and apoptosis were determined using cell counting kit-8, flow cytometry, caspase-3 assay kit, and western blot assays. |
2021-07-19 |
2023-08-13 |
Not clear |
| Zhi Zhang, Huiqing Wen, Bangjian Peng, Jun Weng, Fanhong Zen. CDKN2A deregulation in fatty liver disease and its accelerative role in the process of lipogenesis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 35. issue 4. 2021-07-19. PMID:33769609. |
in the c57bl/6j db/db mouse model and hepatocyte model of fatty liver, the expression pattern of cdkn2a, pias1, protein arginine methyltransferase 1 (prmt1) and casp8 and fadd-like apoptosis regulator (cflar) was characterized by rna quantitative and western blot analysis. |
2021-07-19 |
2023-08-13 |
mouse |
| Lan Ling, Lida Zhi, Haifeng Wang, Yihuan Deng, Chengdong G. MicroRNA-181b Inhibits Inflammatory Response and Reduces Myocardial Injury in Sepsis by Downregulating HMGB1. Inflammation. vol 44. issue 4. 2021-07-19. PMID:34076811. |
the expression of related genes in cells of each group was determined by rt-qpcr and western blot analysis, and the apoptosis rate of transfected cells in each group was determined by tunel assay. |
2021-07-19 |
2023-08-13 |
rat |