| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Huiqiang Chen, Guoshen Xu, You Wu, Xinyu Wang, Fei Wang, Ying Zhan. HBO-PC Promotes Locomotor Recovery by Reducing Apoptosis and Inflammation in SCI Rats: The Role of the mTOR Signaling Pathway. Cellular and molecular neurobiology. vol 41. issue 7. 2021-09-02. PMID:32715402. |
western blot analysis showed that the sci-induced enhanced levels of p-mtor, inflammatory cytokines and apoptosis in the spinal cord were abrogated after administration of hbo-pc. |
2021-09-02 |
2023-08-13 |
rat |
| Yanbin Cao, Lihua Pan, Xuejun Zhang, Wenbin Guo, Dezhang Huan. LncRNA SNHG3 promotes autophagy-induced neuronal cell apoptosis by acting as a ceRNA for miR-485 to up-regulate ATG7 expression. Metabolic brain disease. vol 35. issue 8. 2021-09-02. PMID:32860611. |
lncrna snhg3 and mir-485 or autophagy markers lc3ii/i and beclin-1 expressions were detected by qrt-pcr or western blot and the apoptosis of n2a cells was analyzed by flow cytometry. |
2021-09-02 |
2023-08-13 |
mouse |
| Yanbin Cao, Lihua Pan, Xuejun Zhang, Wenbin Guo, Dezhang Huan. LncRNA SNHG3 promotes autophagy-induced neuronal cell apoptosis by acting as a ceRNA for miR-485 to up-regulate ATG7 expression. Metabolic brain disease. vol 35. issue 8. 2021-09-02. PMID:32860611. |
after the oxygen and glucose deprivation (ogd) treatment of n2a cells transfected with pcdna-snhg3, pcdna-snhg3 + mir-485 mimic for 6 h, 1 mm autophagy inhibitor 3-ma was added and reoxygenated for 24 h, the effect of lncrna snhg3 on the autophagy-induced neuronal cell apoptosis was measured by western blot and flow cytometry. |
2021-09-02 |
2023-08-13 |
mouse |
| Ayaz Ali, Marthandam Asokan Shibu, Chia-Hua Kuo, Jeng-Feng Lo, Ray-Jade Chen, Cecilia Hsuan Day, Tsung-Jung Ho, Vijaya PadmaViswanadha, Wei-Wen Kuo, Chih-Yang Huan. CHIP-overexpressing Wharton's jelly-derived mesenchymal stem cells attenuate hyperglycemia-induced oxidative stress-mediated kidney injuries in diabetic rats. Free radical biology & medicine. vol 173. 2021-09-02. PMID:34298092. |
moreover, western blot analysis, flow cytometry, and mitosox staining revealed that hyperglycemia-induced mitochondrial oxidative stress production and apoptosis were attenuated in chip-overexpressing wharton's jelly-derived mesenchymal stem cells (wjmscs). |
2021-09-02 |
2023-08-13 |
human |
| Min Yi, Dongsheng Wang, Yanyi Chen, Xia Xu, Xingping Da. β-Asarone suppresses TNF-α expression through DNA methylation and c-Jun-mediated transcription modulation in scratch-injured neuronal cells. Journal of biochemical and molecular toxicology. vol 35. issue 7. 2021-09-01. PMID:33969572. |
the neuronal apoptosis was evaluated by terminal deoxynucleotidyl transferase dutp nick end labeling staining and western blot analysis of apoptosis-related proteins. |
2021-09-01 |
2023-08-13 |
mouse |
| Lian Zeng, Fuyu Zhang, Zhen Zhang, Min Xu, Yang Xu, Ying Liu, Hongxia Xu, Xiaodong Sun, Ming Sang, Huiyu Lu. P53 inhibitor pifithrin-α inhibits ropivacaine-induced neuronal apoptosis via the mitochondrial apoptosis pathway. Journal of biochemical and molecular toxicology. vol 35. issue 8. 2021-09-01. PMID:34091999. |
cell viability and apoptosis detected by cck-8 and a jc-1 apoptosis detection kit, the changes of spinal cord structure observed after hematoxylin and eosin staining, apoptosis of the spinal cord measured by terminal deoxynucleotidyl transferase dutp nick end labeling staining, behavioral assessment of the nerve injury evaluated by the detection of sciatic nerve conduction velocity (sncv) andmechanical withdrawal threshold (mwt), the expression of p53 and many apoptosis-related genes included bax, bcl-2, and caspase-3 detected by quantitative real-time polymerase chain reaction, western blot analysis, immunofluorescence, and immunohistochemistry. |
2021-09-01 |
2023-08-13 |
Not clear |
| Sabine Pietkiewicz, Clara Wolfe, Jörn H Buchbinder, Inna N Lavri. Measuring Procaspase-8 and -10 Processing upon Apoptosis Induction. Bio-protocol. vol 7. issue 1. 2021-08-31. PMID:34458412. |
analysing the processing of procaspases-8 and -10 by western blot is a commonly used method to study the induction of apoptosis by death receptor stimulation. |
2021-08-31 |
2023-08-13 |
Not clear |
| Bin Shen, Lan Wang, Yuejun Xu, Hongwei Wang, Shiyi H. Knockdown of lncRNA SNHG15 Ameliorates Oxygen and Glucose Deprivation (OGD)-Induced Neuronal Injury via Regulating the miR-9-5p/TIPARP Axis. Biochemical genetics. 2021-08-28. PMID:34453220. |
besides, the protein levels of apoptosis markers and tccd-induced poly (adp)-ribose polymerase (tiparp) were determined using western blot analysis. |
2021-08-28 |
2023-08-13 |
mouse |
| Zhimin Li, Xing Cao, Ligen Xiao, Ruijiao Zho. Aloperine protects against cerebral ischemia/reperfusion injury via activating the PI3K/AKT signaling pathway in rats. Experimental and therapeutic medicine. vol 22. issue 4. 2021-08-27. PMID:34434259. |
cell apoptosis was assessed by tunel staining, while the expression levels of apoptosis- and pi3k/akt signaling pathway-related proteins were determined by western blot analysis. |
2021-08-27 |
2023-08-13 |
rat |
| Xing-Hua Zhou, Jian Kang, Zhen-Dong Zhong, Yue Chen. Osthole induces apoptosis of the HT-29 cells via endoplasmic reticulum stress and autophagy. Oncology letters. vol 22. issue 4. 2021-08-26. PMID:34429766. |
signaling pathways, including apoptosis, autophagy and ers, were also investigated in the ht-29 cell line using western blot analysis. |
2021-08-26 |
2023-08-13 |
human |
| Yulong Shi, Johannes Greven, Weijun Guo, Peng Luo, Ding Xu, Weikang Wang, Borna Relja, Jan T Vollrath, Eva Miriam Buhl, Klemens Horst, Eftychios Bolierakis, Felix Bläsius, Frank Hildebran. Trauma-Hemorrhage Stimulates Immune Defense, Mitochondrial Dysfunction, Autophagy, and Apoptosis in Pig Liver at 72 h. Shock (Augusta, Ga.). vol 55. issue 5. 2021-08-23. PMID:32826806. |
the leukocyte phenotypes and autophagy and apoptosis pathways were elucidated by immunohistofluorescence, western blot, and terminal deoxynucleotidyl transferase dutp nick-end labeling (tunel).in addition to post-traumatic changes in the mitochondrial morphology, the biomarkers of anti-inflammatory macrophages (cd163) and reparative monocytes (cd11r3 and cd16) were upregulated, while the inducible nitric oxide synthase was downregulated after th. |
2021-08-23 |
2023-08-13 |
Not clear |
| Yunlong Sun, Bo Pang, Yingzhe Wang, Jinglei Xiao, Dacheng Jian. Baohuoside I Inhibits the Proliferation of Hepatocellular Carcinoma Cells via Apoptosis Signaling and NF-kB Pathway. Chemistry & biodiversity. vol 18. issue 6. 2021-08-23. PMID:33904248. |
cell apoptosis was analyzed by flow cytometry, and the levels of cleaved caspase-3, bax, and bcl-2 were quantified by western blot. |
2021-08-23 |
2023-08-13 |
human |
| Wu Wen, Haiyan Wang, Shuang Xie, Zuyin Wu, Chunxu Zhan. BVES-AS1 inhibits the malignant behaviors of colon adenocarcinoma cells via regulating BVES. Cell biology international. vol 45. issue 9. 2021-08-23. PMID:34003551. |
colony formation, cell counting kit-8, jc-1 mitochondrial membrane potential assay, wound healing, transwell, and western blot analyses were used to measure cell proliferation, apoptosis, migration, invasion, and epithelial-mesenchymal transition (emt) in coad cells. |
2021-08-23 |
2023-08-13 |
Not clear |
| Zhen Wang, Changshun Zhong, Yingya Cao, Hongzhen Yin, Guanggui Shen, Weihua Lu, Wei Din. LncRNA DANCR improves the dysfunction of the intestinal barrier and alleviates epithelial injury by targeting the miR-1306-5p/PLK1 axis in sepsis. Cell biology international. vol 45. issue 9. 2021-08-23. PMID:34003569. |
tunel assay and western blot analysis were performed to explore plk1 function in cell apoptosis and intestinal barrier in vitro. |
2021-08-23 |
2023-08-13 |
Not clear |
| Zhen Wang, Changshun Zhong, Yingya Cao, Hongzhen Yin, Guanggui Shen, Weihua Lu, Wei Din. LncRNA DANCR improves the dysfunction of the intestinal barrier and alleviates epithelial injury by targeting the miR-1306-5p/PLK1 axis in sepsis. Cell biology international. vol 45. issue 9. 2021-08-23. PMID:34003569. |
hematoxylin and eosin staining, western blot analysis, and tunel assay were used to investigate dancr function in the intestinal barrier and cell apoptosis in vivo. |
2021-08-23 |
2023-08-13 |
Not clear |
| Shengqiang Fu, Qiaoyun Fan, Juntao Xu, Shengjun Yu, Ming Sun, Yuan Ji, Dun Li. Circ_0008956 contributes to IL-1β-induced osteoarthritis progression via miR-149-5p/NAMPT axis. International immunopharmacology. vol 98. 2021-08-23. PMID:34153672. |
cell viability, apoptosis, cell cycle and extracellular matrix (ecm) degradation were analyzed using cell counting kit-8, flow cytometry, and western blot assays, respectively. |
2021-08-23 |
2023-08-13 |
Not clear |
| Xitao Liu, Defang Zha. CKS1B promotes the progression of hepatocellular carcinoma by activating JAK/STAT3 signal pathway. Animal cells and systems. vol 25. issue 4. 2021-08-20. PMID:34408811. |
the flow cytometry and western blot analysis showed that knockdown of cks1b significantly induced the apoptosis of hep3b and huh7 cells. |
2021-08-20 |
2023-08-13 |
Not clear |
| Zhenbin Li, Xuan Chen, Xiangjun Y. The effects of A549 and H1299 cell-derived exosomes on the proliferation and apoptosis of BEAS-2B cells. Die Pharmazie. vol 76. issue 8. 2021-08-20. PMID:34412737. |
beas-2b cell proliferation, apoptosis and sirt7 protein expression were determined using cck8, flow cytometry and western blot. |
2021-08-20 |
2023-08-13 |
human |
| Lin Zhang, De-Yu Fang, Chun Liu, Dan-Yu Zhao, Yan-Jie Wang, Wen-Na Chen, Yong Cui, Jun-Fu Guo, Pei-Wei Cong, Xiao-Fan Feng, Yun-Ting Zhan. [Mechanism of puerarin reversing SH-SY5Y cell injury induced by Aβ_(1-42) based on proteomics]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 14. 2021-08-18. PMID:34402289. |
puerarin has the anti-alzheimer's disease (ad) activity,which can reverse nerve injury induced by aβand inhibit neuronal apoptosis.however,its potential pharmacodynamic mechanism still needs to be further researched.the occurrence and development of ad is due to the change of multiple metabolic links in the body,which leads to the destruction of balance.puerarin may act on multiple targets and multiple metabolic processes to achieve therapeutic purposes.quantitative proteomic analysis provides a new choice to understand the mechanism as completely as possible.this research adopted sh-sy5y cells induced by aβ_(1-42)to establish ad cell model,and aβimmunofluorescence detection showed that aβdecreased significantly after puerarin intervention.the mechanism of puerarin reversing sh-sy5y cell injured by aβ_(1-42)was further explored by using label-free non-labeled quantitative technology and western blot detection based on bioinformatics analysis result.the results showed that most of the differential proteins were related to biological processes such as cellular component organization or biogenesis,cellular component organization and cellular component biogenesis,and they mainly participated in the top ten pathways of p value such as pathogenic escherichia coli infection,m tor signaling pathway,regulation of autophagy,regulation of actin cytoskeleton,spliceosome,hepatocellular carcinoma,tight junction,non-small cell lung cancer,apoptosis and gap junction.annexin v/pi flow cytometry and tunel were used to detect apoptosis,and the results showed that aβdecreased significantly and the rate of apoptosis decreased significantly after puerarin intervention.western blot analysis found that the protein expression level of autophagy related protein lc3Ⅱwas up-regulated after aβinduction,and the degree of this up-regulation was further enhanced in puerarin intervention group.the trend of the ratio of lc3Ⅱ/lc3Ⅰamong groups was the same as the protein expression level of lc3Ⅱ，the protein expression level of p62 in the control group,ad model group and puerarin intervention group decreased successively.protein interaction network analysis showed that cap1 was correlated with tuba1b,hsp90ab2p,dnm1l,tuba1a and erk1/2,and the correlation between cap1 and erk1/2 was the highest among them.western blot showed that the expressions of p-erk1/2,bax and cap1 were significantly down-regulated and the protein expression level of bcl-2 was significantly up-regulated after puerarin intervention.therefore,puerarin might improve the sh-sy5y cells injured by aβ_(1-42)through the interaction of multiple biological processes and pathways in cells multiple locations,and cap1 might play an important role among them. |
2021-08-18 |
2023-08-13 |
Not clear |
| He Yu, Zhong-Mei He, Kun Shi, Yan Zhao, Ying Zong, Jian-Ming Li, Wei-Jia Chen, Rui D. [Study on antidepressant effect of total alkaloids of Fibraurea recisa]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 14. 2021-08-18. PMID:34402292. |
this study aimed to investigate the antidepressant effects of total alkaloids of fibraurea recisa in ht22 cells damaged by corticosterone (cort) in vitro and in a mouse model of chronic unpredictable mild stress (cums) as well as the underlying mechanisms.in cellular experiments,the viability of cort-damaged ht22 cells was detected using cell counting kit-8 (cck-8),and the cell apoptosis was detected by hoechst 33258 staining.in animal experiments,c57bl/6n mice were randomly divided into the control group,model group,low (100 mg·kg~(-1)),medium (200 mg·kg~(-1)) and high (400 mg·kg~(-1))-dose of total alkaloids of f.recisa groups,and positive control group.after 21 days of cums exposure,their depressive behaviors were observed in behavioral and morris water maze tests.the serum levels of 5-hydroxytryptamine (5-ht),dopamine (da),and norepinephrine (ne) were assessed by elisa.the expression levels of apoptosis-related proteins bcl-2,bax and cleaved caspase-3 in ht22 cells and mouse hippocampus were detected by western blot.the results suggested that total alkaloids of f.recisa alleviated the damage of ht22 cells induced by cort in a dose-dependent manner.the hoechst 33258 staining uncovered that total alkaloids of f.recisa better reduced the blue spots and inhibited cell apoptosis.the results of animal experiments showed that total alkaloids of f.recisa significantly improved the depression-like behaviors of mice and increased the serum levels of 5-ht,da and ne as compared with those in the model group.the western blot assays revealed a significant up-regulation of bcl-2 protein expression,but an obvious reduction in bax and cleaved caspase-3protein expression in the total alkaloids of f.recisa group.in conclusion,total alkaloids of f.recisa inhibited depression possibly by regulating the apoptosis-related protein expression or elevating the monoamine neurotransmitter levels in the brain. |
2021-08-18 |
2023-08-13 |
mouse |