| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Lai Chen, Shaohui Zhang, Xiaoying Liu, Jiong Dai, Zhenguo Yan, Chunlan W. Effects of dendrobium polysaccharides on human brain microvascular endothelial cell injury induced by ox-LDL via regulating the miR-378 expression. Cellular and molecular biology (Noisy-le-Grand, France). vol 66. issue 7. 2021-08-06. PMID:33287924. |
the kit was used to detect the levels of malondialdehyde (mda) and the activities of superoxide dismutase (sod) and catalase (cat); flow cytometry to detect apoptosis; and western blot to detect b-cell lymph tumor/leukemia-2 (bcl-2) and bcl-2 related x (bax) protein expression; real-time fluorescence quantitative pcr (rt-qpcr) was used to detect the expression of mir-378. |
2021-08-06 |
2023-08-13 |
human |
| Kuo Wang, Nan Zhang, Zhanbiao Yu, Ning L. Galactose protects pancreatic acinar cells from cerulein induced damage by regulating FGF21 and Klotho. Pakistan journal of pharmaceutical sciences. vol 33. issue 3(Special). 2021-08-06. PMID:33361031. |
acute pancreatitis cell injury model was established by arbusin-induced pancreatic acinar cell ar42j injury; galactose (25, 50, 100 mmol / l) was used to treat the injured cells, and the optimal concentration was 50 mmol / l; cell counting kit (cck-8), enzyme linked immunosorbent assay (elisa) to detect cell survival rate and necrosis rate; flow cytometry and western blotting (western blot) to detect cell apoptosis and autologous phage-related gene (beclin1) and microtubule-associated protein 1 light chain 3 (lc3), apoptosis-related protein b-cell lymphoma / leukemia-2 (bcl-2), bcl-2-related x gene (bax), and fibroblasts expression of growth factor 21 antibody (fgf21) and anti-aging gene klotho. |
2021-08-06 |
2023-08-13 |
Not clear |
| Fei Wang, Xin Min, Shan-You Hu, Da-Li You, Ting-Ting Jiang, Li Wang, Xiao W. Hypoxia/reoxygenation-induced upregulation of miRNA-542-5p aggravated cardiomyocyte injury by repressing autophagy. Human cell. vol 34. issue 2. 2021-08-06. PMID:33394350. |
the role of mirna and autophagy in regulating the viability and cell apoptosis was evaluated using cell counting kit-8 (cck-8) assay, flow cytometry (fcm), and western blot. |
2021-08-06 |
2023-08-13 |
Not clear |
| Hui Shi, Ying Yu, Yucheng Wang, Xiaoxiao Liu, Yong Yu, Minghui Li, Yunzeng Zou, Ruizhen Chen, Junbo G. Inhibition of Calpain Alleviates Apoptosis in Coxsackievirus B3-induced Acute Virus Myocarditis Through Suppressing Endoplasmic Reticulum Stress. International heart journal. vol 62. issue 4. 2021-08-06. PMID:34234076. |
apoptosis was measured by tunel staining, flow cytometry, and western blot. |
2021-08-06 |
2023-08-13 |
mouse |
| Rong Ding, Wenyue Kang, Duozhi Wu, Lin Wan. Protective effect of propofol via the regulation of ovarian granulosa cell proliferation and apoptosis. Experimental and therapeutic medicine. vol 22. issue 3. 2021-08-06. PMID:34345270. |
kgn cells were transfected with mir-451a inhibitor or inhibitor control sequences for 6 h and treated with 10 µg/ml propofol for an additional 48 h. the results from the mtt, rt-qpcr and western blot assays indicated that 10 µg/ml propofol inhibited cell viability, induced apoptosis, enhanced cleaved caspase 3 expression, reduced pro-caspase 3 levels and inhibited the protein and mrna expression of wnt3a and β-catenin. |
2021-08-06 |
2023-08-13 |
human |
| Guang Yang, Christopher Qian, Chao Zhang, Yong Bao, Meng-Yue Liu, Fei Jiang, Wei Li, Yong Liu, Ya Ke, Zhong-Ming Qia. Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats. Translational research : the journal of laboratory and clinical medicine. vol 229. 2021-08-05. PMID:32932001. |
here, we examined effects of ad-hepcidin (hepcidin expression adenovirus) on the nonheme iron contents, expression of hepcidin, ferritin and iron transport proteins, neuronal cell survival, water contents in the brain and/or cerebrospinal fluid (csf), and ich-induced apoptosis, neurological deficit by rt-pcr, western blot analysis, neun immunofluorescence, tunel, fluoro-jade b staining, behavioral performance and morris water-maze tests in 510 rats. |
2021-08-05 |
2023-08-13 |
rat |
| Xianglun Cui, Yanhan Dong, Mingyu Li, Xuekang Wang, Meiqing Jiang, Weiwei Yang, Gege Liu, Shuqi Sun, Wenhua X. A circular RNA from NFIX facilitates oxidative stress-induced H9c2 cells apoptosis. In vitro cellular & developmental biology. Animal. vol 56. issue 9. 2021-08-04. PMID:33067659. |
the biological function of circnfix in cardiomyocytes was investigated through loss- and gain-of-function assays, and cardiomyocyte apoptosis level was detected by the terminal deoxyribonucleotidyl transferase-mediated tdt-mediated dutp nick end labeling assay and western blot. |
2021-08-04 |
2023-08-13 |
mouse |
| Fang Fu, Lu-Shan Li, Ru Li, Qiong Deng, Qiu-Xia Yu, Xin Yang, Min Pan, Jin Han, Li Zhen, Li-Na Zhang, Ting-Ying Lei, Dong-Zhi Li, Can Lia. All-trans-retinoid acid induces the differentiation of P19 cells into neurons involved in the PI3K/Akt/GSK3β signaling pathway. Journal of cellular biochemistry. vol 121. issue 11. 2021-08-03. PMID:31961017. |
real-time quantitative polymerase chain reaction and western blot analysis were used to determine the expression of neuronal-specific markers, whereas flow cytometry was used to analyze cell cycle and cell apoptosis. |
2021-08-03 |
2023-08-13 |
mouse |
| Yuyang Zhang, Kate Huang, Huihua Cai, Siping Chen, Donghua Sun, Pengfei Jian. The role of nucleolar spindle-associated protein 1 in human ovarian cancer. Journal of cellular biochemistry. vol 121. issue 11. 2021-08-03. PMID:32031285. |
after knocking down nusap1 with lentivirus-mediated shrna and verifying the knockdown efficiency via quantitative real-time polymerase chain reaction and western blot assays, the cell proliferation, apoptosis, and cell cycle were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, and flow cytometry, respectively. |
2021-08-03 |
2023-08-13 |
human |
| Hongwen You, Xinyou Su, Guoying S. Novel thiazole-pyrazolone hybrids as potent ACE inhibitors and their cardioprotective effect on isoproterenol-induced myocardial infarction. Archiv der Pharmazie. vol 353. issue 12. 2021-08-03. PMID:32841430. |
in the western blot analysis, compound 4i prevented mitochondrial apoptosis after mi by downregulating the expression of cleaved caspase-3 and bax, with the upregulation of bcl-2, as compared with the iso group. |
2021-08-03 |
2023-08-13 |
rat |
| Syed Riaz Ud Din, Muhammad Azhar Nisar, Muhammad Noman Ramzan, Muhammad Zubair Saleem, Hassan Ghayas, Bashir Ahmad, Samana Batool, Kashif Kifayat, Xiaorong Guo, Min Huang, Mintao Zhon. Latcripin-7A from Lentinula edodes C European journal of pharmacology. vol 907. 2021-08-03. PMID:34224698. |
apoptosis of lp-7a treated g.c cells was evaluated via hoechst stain, western blot and flow cytometry. |
2021-08-03 |
2023-08-13 |
Not clear |
| Kailiang Zhou, Huanwen Chen, Huazi Xu, Xiaofeng Ji. Trehalose Augments Neuron Survival and Improves Recovery from Spinal Cord Injury via mTOR-Independent Activation of Autophagy. Oxidative medicine and cellular longevity. vol 2021. 2021-08-03. PMID:34336117. |
h&e staining, nissl staining, western blot, and immunofluorescence studies were conducted to examine nerve morphology and quantify autophagy and mitochondrial-dependent apoptosis at various time points after surgery. |
2021-08-03 |
2023-08-13 |
rat |
| Qifeng Yu, Wei Zhang, Xin Zhou, Wenqi Shen, Chungen Xing, Xiaodong Yan. Regulation of lnc-TLCD2-1 on Radiation Sensitivity of Colorectal Cancer and Comprehensive Analysis of Its Mechanism. Frontiers in oncology. vol 11. 2021-08-03. PMID:34336703. |
cell clone formation assay, flow cytometry, and cell counting kit 8 (cck8) were used to detect radiation sensitivity, apoptosis, and proliferation of colorectal cancer cells, respectively; quantitative real-time pcr and western blot were used to detect the expression of genes; the direct interaction between lnc-tlcd2-1 and hsa-mir-193a-5p was verified by dual luciferase reporter assays; gepia, starbase, timer and david were used to complete expression of lnc-tlcd2-1, mir-193a-5p,yy1 and nf-кb-p65 in colorectal cancer, correlation, immune cell infiltration, go and kegg enrichment analysis. |
2021-08-03 |
2023-08-13 |
Not clear |
| Li-Juan Wang, Han-Qing Ca. Let-7b downgrades CCND1 to repress osteogenic proliferation and differentiation of MC3T3-E1 cells: An implication in osteoporosis. The Kaohsiung journal of medical sciences. vol 36. issue 10. 2021-08-02. PMID:32533643. |
the expression of let-7b in mc3t3-e1 and its effect on cell viability, apoptosis, and the apoptosis-related proteins (bcl-2, bax, and cleaved caspase-9) were tested by cck-8 assay, flow cytometry and western blot, severally. |
2021-08-02 |
2023-08-13 |
Not clear |
| Wei Dong, Hongxia Yang, Minghua Cheng, Xin Zhang, Jingjing Yin, Zhaodong Zeng, Guang Huan. Dexmedetomidine alleviates pulmonary ischemia-reperfusion injury through modulating the miR-21-5p/Nr4a1 signaling pathway. Acta biochimica Polonica. vol 67. issue 4. 2021-08-02. PMID:33332077. |
the h/r-induced mpvecs were exposed to dex, and the cell viability, cell apoptosis and protein expressions were assessed by the cell counting kit-8 (cck8) assay, flow cytometry and western blot, respectively. |
2021-08-02 |
2023-08-13 |
mouse |
| Liang Pei, Linxi H. Hepatoprotective effect of anemoside B4 against sepsis-induced acute liver injury through modulating the mTOR/p70S6K-mediated autophagy. Chemico-biological interactions. vol 345. 2021-08-02. PMID:34051206. |
tunel staining and western blot revealed that anemoside b4 suppressed cell apoptosis, along with decreased bax, leaved caspase-3, cleaved parp, but increased bcl-2. |
2021-08-02 |
2023-08-13 |
mouse |
| Mingming Tan, Rongxin Be. Circ_0091702 serves as a sponge of miR-545-3p to attenuate sepsis-related acute kidney injury by upregulating THBS2. Journal of molecular histology. vol 52. issue 4. 2021-08-02. PMID:34101064. |
besides, the protein levels of apoptosis markers and thbs2 were evaluated by western blot analysis. |
2021-08-02 |
2023-08-13 |
human |
| Lining Wang, Jingwen Zhang, Chengtian Zhao, Zhenzhen Jia, Xizeng Fen. Melatonin Reverses 10-Hydroxycamptothecin-Induced Apoptosis and Autophagy in Mouse Oocyte. Reproductive sciences (Thousand Oaks, Calif.). vol 28. issue 7. 2021-07-30. PMID:33104985. |
the apoptosis and autophagy levels of oocytes in the hcpt-treated group were detected by qrt-pcr and western blot. |
2021-07-30 |
2023-08-13 |
mouse |
| Shokoofeh Kazemzadeh, Tayebeh Rastegar, Bagher Minaei Zangi, Mehrnoush Malekzadeh, Maryam Khanehzad, Parastoo Khanlari, Soheila Madadi, Alieh Bashghareh, Azim Hedayatpou. Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion. Reproductive sciences (Thousand Oaks, Calif.). vol 28. issue 5. 2021-07-30. PMID:33481217. |
the thawed sscs were evaluated for survival, apoptosis, and ros level before transplantation, and the proliferation (mvh and id4) and differentiation (c-kit, scp3, tp1, tp2, and prm1) markers of sscs were examined using immunofluorescence, western blot, and quantitative real-time polymerase chain reaction (pcr) after transplantation. |
2021-07-30 |
2023-08-13 |
mouse |
| Meng Lan, Zhaodi Kong, Fengjie Liu, Tengteng Zou, Lihong Li, Tiange Cai, Tian Huaqin, Yu Ca. Activating Caspase-8/Bid/ROS Signaling to Promote Apoptosis of Breast Cancer Cells by Folate-modified Albumin Baicalin-loaded Nanoparticles. Nanotechnology. 2021-07-30. PMID:34330116. |
flow cytometry and western blot confirmed that it promoted apoptosis by increasing the expression of caspase-8 and ros, and decreasing the level of bid. |
2021-07-30 |
2023-08-13 |
Not clear |