| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Lianggeng Xiong, Binxin Tan, Xiubing Lei, Biao Zhang, Wenting Li, Daimei Liu, Tian Xi. SIRT6 through PI3K/Akt/mTOR signaling pathway to enhance radiosensitivity of non-Small cell lung cancer and inhibit tumor progression. IUBMB life. vol 73. issue 9. 2021-10-15. PMID:34033225. |
then, check the cell proliferation, migration, apoptosis and cell cycle by mtt, wound healing assay and flow cytometry, while detect the expression of pi3k/akt/mtor signaling pathway-related proteins by western blot. |
2021-10-15 |
2023-08-13 |
mouse |
| Zhenhua Zhang, Yidi Zhang, Chunju Yang, Qianyu Wang, Hong Wang, Yanting Zhang, Wenbin Deng, Yichu Nie, Yonghong Liu, Xiaowei Luo, Jie Huang, Junjian Wan. Antitumor effects of 3-bromoascochlorin on small cell lung cancer via inhibiting MAPK pathway. Cell biology international. vol 45. issue 11. 2021-10-15. PMID:34288235. |
then western blot analysis (wb) and flow cytometry were conducted, and we found bas could induce the apoptosis of h446 and h69ar cells. |
2021-10-15 |
2023-08-13 |
mouse |
| Chujie Zhang, Huimei Yu, Han Yang, Ben Li. Activation of PI3K/PKB/GSK-3β signaling by sciadopitysin protects cardiomyocytes against high glucose-induced oxidative stress and apoptosis. Journal of biochemical and molecular toxicology. vol 35. issue 10. 2021-10-15. PMID:34392578. |
apoptosis was assessed by flow cytometry analysis, caspase-3/7 activity assay, and western blot analysis of cytochrome c (cyt c) expression. |
2021-10-15 |
2023-08-13 |
cat |
| Qiang Zuo, Yunsheng Ou, Shenxi Zhong, Haoyang Yu, Fangbiao Zhan, Muzi Zhan. Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway. Acta biochimica et biophysica Sinica. vol 53. issue 10. 2021-10-15. PMID:34494093. |
apoptosis and reactive oxygen species (ros) levels were detected by hoechst staining and flow cytometry, cell viability was detected by cell counting kit-8 assay, grp78 protein fluorescence intensity was detected by immunofluorescence, and apoptosis-related proteins, cell proliferation-related proteins, and wnt pathway-related proteins were detected by western blot. |
2021-10-15 |
2023-08-13 |
human |
| Hui Yang, Jian Lin, Jinling Jiang, Jun Ji, Chao Wang, Jun Zhan. miR-20b-5p functions as tumor suppressor microRNA by targeting cyclinD1 in colon cancer. Cell cycle (Georgetown, Tex.). vol 19. issue 21. 2021-10-13. PMID:33044899. |
to gain further mechanism insight, flow cytometry was performed to determine cell apoptosis and cell cycle, and western blot or immunohistochemistry were employed to check the expression of ccnd1/cdk/foxm1 axis in hct116 cells. |
2021-10-13 |
2023-08-13 |
mouse |
| Chien-Yu Huang, Yu-Jia Chang, Po-Li Wei, Chin-Sheng Hung, Weu Wan. Methyl gallate, gallic acid-derived compound, inhibit cell proliferation through increasing ROS production and apoptosis in hepatocellular carcinoma cells. PloS one. vol 16. issue 3. 2021-10-13. PMID:33725002. |
finally, apoptosis was examined using annexin v-fitc/pi staining and western blot was performed to determine the molecular mechanism. |
2021-10-13 |
2023-08-13 |
human |
| Chien-Yu Huang, Yu-Jia Chang, Po-Li Wei, Chin-Sheng Hung, Weu Wan. Methyl gallate, gallic acid-derived compound, inhibit cell proliferation through increasing ROS production and apoptosis in hepatocellular carcinoma cells. PloS one. vol 16. issue 3. 2021-10-13. PMID:33725002. |
the western blot analysis showed that mg activated cleavage of caspase-3 and poly (sdp ribose) polymerase (parp), modulated levels of the bcl2, bax, and bad ligands, and induced apoptosis. |
2021-10-13 |
2023-08-13 |
human |
| Hui Geng, Guangli Liu, Jiandong Hu, Jianchao Li, Dong Wang, Shenshan Zou, Xintao X. HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway. Molecular medicine reports. vol 24. issue 4. 2021-10-13. PMID:34396425. |
additionally, cell apoptosis was assessed using tunel staining, and the expression of apoptosis‑related proteins was detected by western blot analysis. |
2021-10-13 |
2023-08-13 |
human |
| Jia Wang, Aolin Du, Hexilin Wang, Yang L. MiR-599 regulates LPS-mediated apoptosis and inflammatory responses through the JAK2/STAT3 signalling pathway via targeting ROCK1 in human umbilical vein endothelial cells. Clinical and experimental pharmacology & physiology. vol 47. issue 8. 2021-10-12. PMID:32248560. |
cell viability was analyzed by cck-8 assay and trypan blue exclusion assay; the formation of dna fragments was tested by cell death detection elisa plus kit; the incidence of apoptosis was detected by flow cytometry; the expression of p53 and cleaved-caspase 3 (c-caspase 3) was evaluated by western blot. |
2021-10-12 |
2023-08-13 |
human |
| Haiqiao Xu, Shibang Lin, Haizhou Huan. Involvement of increased expression of chemokine C-C motif chemokine 22 (CCL22)/CC chemokine receptor 4 (CCR4) in the inflammatory injury and cartilage degradation of chondrocytes. Cytotechnology. vol 73. issue 5. 2021-10-12. PMID:34629747. |
additionally, cell apoptosis as well as its related proteins was detected by tunel and western blot, respectively. |
2021-10-12 |
2023-08-13 |
Not clear |
| Bin Li, Xinyong Cai, Yunxia Wang, Hongmin Zhu, Ping Zhang, Panpan Jiang, Xu Yang, Jianhua Sun, Lang Hong, Liang Sha. Circ-SKA3 Enhances Doxorubicin Toxicity in AC16 Cells Through miR-1303/TLR4 Axis. International heart journal. vol 62. issue 5. 2021-10-11. PMID:34544967. |
the viability and apoptosis of ac16 cells were analyzed using cell counting kit-8, flow cytometry, and western blot assays. |
2021-10-11 |
2023-08-13 |
Not clear |
| Guohui Yan, Jiajia Wang, Zanxi Fang, Shuidi Yan, Yang Zhan. MiR-26a-5p Targets WNT5A to Protect Cardiomyocytes from Injury Due to Hypoxia/Reoxygenation Through the Wnt/β-catenin Signaling Pathway. International heart journal. vol 62. issue 5. 2021-10-11. PMID:34544974. |
the viability and the apoptosis rate of cells in each group were detected using cck-8 and flow cytometry; the relationship between mir-26a-5p and wnt5a was verified by a dual-luciferase reporter assay; the expression of mir-26a-5p, wnt5a, cleavedcaspase3 and wnt/β-catenin signaling pathway-related proteins in each group was detected using qrt-pcr or western blot; ldh release, sod, and gsh-px activities in each group were detected by kit.in the h/r group, the expression level of mir-26a-5p was significantly decreased, whereas the expression level of wnt5a was significantly increased. |
2021-10-11 |
2023-08-13 |
rat |
| Zhuoqun Fang, Dongliang Zhang, Li Yao, Gaofeng Wu, Zhi Zhang, Xudong Deng, Juntao Han, Xuekang Yan. Burn-Induced Apoptosis of Pulmonary Microvascular Endothelial Cell is NHE1 Dependent and Regulated by PI3K-Akt and p38 MAPK Pathways. Shock (Augusta, Ga.). vol 54. issue 6. 2021-10-06. PMID:32496418. |
based on the western blot analyses, real-time pcr, fluorescence spectroscopy, and apoptosis analysis, we found that burn serum significantly induced nhe1 activation, promoted intracellular na accumulation, and elevated apoptosis ratio. |
2021-10-06 |
2023-08-13 |
human |
| Jingbo Zhou, Shu Zhang, Xinyi Sun, Yan Lou, Jinjing Bao, Jiangyi Y. Hyperoside ameliorates diabetic nephropathy induced by STZ via targeting the miR-499-5p/APC axis. Journal of pharmacological sciences. vol 146. issue 1. 2021-10-06. PMID:33858650. |
western blot analysis was conducted for detection of extracellular matrix accumulation, inflammatory response and cell apoptosis. |
2021-10-06 |
2023-08-13 |
mouse |
| Sutthiwan Janthamala, Apinya Jusakul, Sarinya Kongpetch, Phongsaran Kimawaha, Poramate Klanrit, Watcharin Loilome, Nisana Namwat, Anchalee Techase. Arctigenin inhibits cholangiocarcinoma progression by regulating cell migration and cell viability via the N-cadherin and apoptosis pathway. Naunyn-Schmiedeberg's archives of pharmacology. vol 394. issue 10. 2021-10-05. PMID:34283274. |
furthermore, flow cytometry and western blot analysis revealed that arctigenin significantly reduced cca cell viability and induced apoptosis via the bax/bcl-2/caspase-3 pathway. |
2021-10-05 |
2023-08-13 |
Not clear |
| Bella Shadur, Nathalie Asherie, Shlomit Kfir-Erenfeld, Taly Dubnikov, Adeeb NaserEddin, Yael Dinur Schejter, Orly Elpeleg, Hagar Mor-Shaked, Polina Stepensk. A human case of GIMAP6 deficiency: a novel primary immune deficiency. European journal of human genetics : EJHG. vol 29. issue 4. 2021-10-04. PMID:33328581. |
in order to interrogate the patients' immune defect, we performed whole-exome sequencing, western blot, flow cytometry analysis, lymphocyte activation and proliferation studies, cytokine release assays, and apoptosis studies. |
2021-10-04 |
2023-08-13 |
mouse |
| Yan Hou, Junjie Sun, Jie Huang, Fengzhi Yao, Xuelian Chen, Bin Zhu, Dongchi Zha. Circular RNA circRNA_0000094 sponges microRNA-223-3p and up-regulate F-box and WD repeat domain containing 7 to restrain T cell acute lymphoblastic leukemia progression. Human cell. vol 34. issue 3. 2021-10-04. PMID:33677796. |
in this work, quantitative real-time polymerase chain reaction (qrt-pcr) was applied to quantify circ_0000094, mir-223-3p, and f-box and wd repeat domain containing 7 (fbw7) mrna expressions in lymph node samples from t-all patients; western blot was adopted to examine fbw7 protein expression in t-all cells; cell proliferation was detected by cell counting kit-8 (cck-8) experiment; apoptosis was examined by flow cytometry; transwell experiments were applied to assess t-all cell migration and invasion; the interactions among circ_0000094 and mir-223-3p, and mir-223-3p and fbw7 were validated by bioinformatics prediction, dual-luciferase reporter gene assay, and rna immunoprecipitation experiment. |
2021-10-04 |
2023-08-13 |
Not clear |
| Congshan Li, Panpan Yang, Bo Liu, Jie Bu, Hongrui Liu, Jie Guo, Tomoka Hasegawa, Haipeng Si, Minqi L. Prednisolone induces osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway. Journal of molecular histology. vol 52. issue 5. 2021-10-04. PMID:34297260. |
hoechst 33342/pi staining, flow cytometry, rt-pcr, and western blot were used to detect osteocytes apoptosis, sirna transfection efficiency, and expressions of pathway related factors. |
2021-10-04 |
2023-08-13 |
mouse |
| Cagla Kayabasi, Besra Ozmen Yelken, Aycan Asik, Tugce Balci Okcanoglu, Fatma Sogutlu, Roya Gasimli, Sunde Yilmaz Susluer, Guray Saydam, Cigir Biray Avci, Cumhur Gundu. PI3K/mTOR dual-inhibition with VS-5584 enhances anti-leukemic efficacy of ponatinib in blasts and Ph-negative LSCs of chronic myeloid leukemia. European journal of pharmacology. vol 910. 2021-10-04. PMID:34461124. |
we investigated the effects of inhibitory concentrations on apoptosis, cell viability and cell-cycle regulation (flow cytometry), protein levels (elisa, western blot), transcriptional activities (dual-luciferase reporter assay), gene expressions (qrt-pcr). |
2021-10-04 |
2023-08-13 |
Not clear |
| Mingliang Xu, Jianguo Feng, Mingxi Tang, Qingxi Guo, Jian Zhan, Fuzu Zhu, Hong Lei, Qingmei Kan. Blocking retrograde axonal transport of autophagosomes contributes to sevoflurane-induced neuron apoptosis in APP/PS1 mice. Acta neurologica Belgica. vol 121. issue 5. 2021-10-02. PMID:32385796. |
morris water maze experiment was used to test the learning and memory abilities of mice, flow cytometry was conducted to detect apoptosis and mitochondrial membrane potential of brain cells in mice, transmission electron microscopy was used to observe the number of autophagosomes at the axon in mice, and western blot was carried out to detect the expression of bax, bcl-2, lc3ii, p62, kif3b and dic proteins of brain cells in mice. |
2021-10-02 |
2023-08-13 |
mouse |