All Relations between apoptosis and blot

Publication Sentence Publish Date Extraction Date Species
Sahar Sadegh-Nejadi, Reza Afrisham, Solaleh Emamgholipour, Pantea Izadi, Neda Eivazi, Behnoosh Tahbazlahafi, Maliheh Pakneja. Influence of plasma circulating exosomes obtained from obese women on tumorigenesis and tamoxifen resistance in MCF-7 cells. IUBMB life. vol 72. issue 9. 2021-11-22. PMID:32542981. for evaluating resistance to tamoxifen, the cell viability, apoptosis, and the p53 protein were evaluated using the mtt assay, flow cytometry, and western blot methods, respectively. 2021-11-22 2023-08-13 Not clear
Haihua Huang, Huiyun Liu, Jiazhen Tang, Wenqiong Xu, Huaxia Gan, Qiwei Fan, Wei Zhan. M2 macrophage-derived exosomal miR-25-3p improves high glucose-induced podocytes injury through activation autophagy via inhibiting DUSP1 expression. IUBMB life. vol 72. issue 12. 2021-11-22. PMID:33107695. annexin v-fitc/pi double staining was performed to measure podocytes apoptosis, and western blot was carried out to ensure proteins expression. 2021-11-22 2023-08-13 Not clear
Wanjing Ning, Miao Huang, Shengming Wu, Hong Wang, Jiali Yao, Yingying Ge, Yulu Tang, Kejian Sun, Xiaoxun Xie, Qiping H. CT23 knockdown attenuating malignant behaviors of hepatocellular carcinoma cell is associated with upregulation of metallothionein 1. Cell biology international. vol 45. issue 6. 2021-11-22. PMID:33559934. furthermore, western blot analysis and cell behaviors assays verified that ct23 modulates cell proliferation, migration, and apoptosis by regulating mt1 expression in hcc cells and non-neoplastic hepatocytes. 2021-11-22 2023-08-13 Not clear
Chaofeng Wei, Shan Xiang, Yi Yu, Jingyan Song, Mingming Zheng, Fang Lia. miR-221-3p regulates apoptosis of ovarian granulosa cells via targeting FOXO1 in older women with diminished ovarian reserve (DOR). Molecular reproduction and development. vol 88. issue 4. 2021-11-22. PMID:33694202. following transfection, apoptosis of granulosa cells was determined by flow cytometry, and the expression of the target gene was measured by quantitative real-time polymerase chain reaction (qrt-pcr) and western blot analysis (wb). 2021-11-22 2023-08-13 Not clear
Xinyu Zhou, Zhonglong Wang, Bingchao Xu, Niu Ji, Pin Meng, Lei Gu, Ying L. Long non-coding RNA NORAD protects against cerebral ischemia/reperfusion injury induced brain damage, cell apoptosis, oxidative stress and inflammation by regulating miR-30a-5p/YWHAG. Bioengineered. vol 12. issue 2. 2021-11-22. PMID:34709972. additionally, cell viability was determined using cck-8 assay, and cell apoptosis was assessed using tunel staining and western blot analysis. 2021-11-22 2023-08-13 Not clear
Fu-Jing Liu, Ti-Jun Gu, Dong-Yue We. Emodin alleviates sepsis-mediated lung injury via inhibition and reduction of NF-kB and HMGB1 pathways mediated by SIRT1. The Kaohsiung journal of medical sciences. 2021-11-22. PMID:34806822. murine alveolar epithelial cell line (mle-12) cells were used for flow cytometry and western blot to analyze the apoptosis rate and protein expression. 2021-11-22 2023-08-13 rat
Elisabeth Bankell, Sara Dahl, Olof Gidlöf, Daniel Svensson, Bengt-Olof Nilsso. LL-37-induced caspase-independent apoptosis is associated with plasma membrane permeabilization in human osteoblast-like cells. Peptides. vol 135. 2021-11-19. PMID:33129893. apoptosis was assessed by the terminal deoxynucleotidyl dutp nick end labeling (tunel) assay and annexin v flow cytometry, and caspase-3 and poly (adp-ribose) polymerase (parp) cleavage were determined by western blot. 2021-11-19 2023-08-13 human
Qiu Li, Yuan Bian, Qiaolian L. Down-Regulation of TMPO-AS1 Induces Apoptosis in Lung Carcinoma Cells by Regulating miR-143-3p/CDK1 Axis. Technology in cancer research & treatment. vol 20. 2021-11-19. PMID:33685293. western blot experiments were used to examine the expressions of apoptosis-related proteins, and flow cytometry tested the cell apoptosis in treated cells. 2021-11-19 2023-08-13 Not clear
Dahae Lee, Jae Sik Yu, Rhim Ryoo, Jin-Chul Kim, Tae Su Jang, Ki Sung Kang, Ki Hyun Ki. Pulveraven A from the fruiting bodies of Pulveroboletus ravenelii induces apoptosis in breast cancer cell via extrinsic apoptotic signaling pathway. The Journal of antibiotics. vol 74. issue 10. 2021-11-19. PMID:34172938. annexin v alexa fluor 488 binding assay and western blot results revealed that pulveraven a induced apoptotic cell death via the extrinsic apoptosis pathway, as indicated by the activation of initiator caspase-8 and executioner caspase-7. 2021-11-19 2023-08-13 human
Min Hu, Tian Gao, Ying D. MiR-98-3p regulates ovarian granulosa cell proliferation and apoptosis in polycystic ovary syndrome by targeting YY1. Medical molecular morphology. 2021-11-19. PMID:34796378. the viability, cell cycle, and apoptosis of ovarian granulosa cells (ogcs) were assessed by cck-8, flow cytometry, and western blot. 2021-11-19 2023-08-13 mouse
Fu Hua, Chang-Hua Li, Xiao-Gang Chen, Xiao-Ping Li. [Retracted] Daidzein exerts anticancer activity towards SKOV3 human ovarian cancer cells by inducing apoptosis and cell cycle arrest, and inhibiting the Raf/MEK/ERK cascade. International journal of molecular medicine. vol 47. issue 6. 2021-11-18. PMID:33846770. following the publication of the above paper, a concerned reader drew to the editor's attention that several figures contained data that bore striking similarities to data published in other papers; notably, the western blot data shown in fig. 6 appeared to have been presented in other studies, notably in fig. 7b of another paper published around the same time and written by different authors based at different research institutions [li p, zhang z, zhang f, zhou h and sun w: effects of 3‑tetrazolyl methyl‑3‑hydroxy‑oxindole hybrid (thoh) on cell proliferation, apoptosis, and g2/m cell cycle arrest occurs by targeting platelet‑derived growth factor d (pdgf‑d) and the mek/erk signaling pathway in human lung cell lines sk‑lu‑1, a549, and a‑427. 2021-11-18 2023-08-13 human
Pengyi Xing, Ye Wang, Li Zhang, Chao Ma, Jianping L. Knockdown of lncRNA MIR4435‑2HG and ST8SIA1 expression inhibits the proliferation, invasion and migration of prostate cancer cells International journal of molecular medicine. vol 47. issue 6. 2021-11-18. PMID:33846784. the expression levels of proteins related to proliferation, apoptosis, invasion and migration, and the fak/akt/β‑catenin pathway were detected by western blot analysis. 2021-11-18 2023-08-13 mouse
Wu Chen, Sheng Zhao, Weimin Yu, Ting Rao, Yuan Ruan, Shaoming Zhu, Yuqi Xia, Hongfei Song, Fan Chen. SC66 inhibits the proliferation and induces apoptosis of human bladder cancer cells by targeting the AKT/β-catenin pathway. Journal of cellular and molecular medicine. vol 25. issue 22. 2021-11-18. PMID:34687144. western blot analysis and immunohistochemistry staining confirmed that sc66 inhibited the emt process in vivo and induced apoptosis through the akt/β-catenin signalling pathway. 2021-11-18 2023-08-13 mouse
Qing Wang, Lujing Ying, Bing Wei, Yikui Ji, Yi X. Effects of quercetin on apoptosis and extracellular matrix degradation of chondrocytes induced by oxidative stress-mediated pyroptosis. Journal of biochemical and molecular toxicology. 2021-11-18. PMID:34791735. cell counting kit-8 (cck-8 kit), flow cytometry, enzyme-linked immunosorbent assay (elisa) kit, western blot, dichlorodihydrofluorescein diacetate (dcfh-da) kit, thiobarbituric acid (tba) test, toluidine blue staining, hematoxylin eosin (he) staining and terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick-end labeling (tunel) staining were used to evaluate cell viability, cell apoptosis, inflammatory cytokines level, protein expression, reactive oxygen species (ros) level, malondialdehyde (mda) content, morphological changes, and chondrocyte apoptosis of cartilage, respectively. 2021-11-18 2023-08-13 rat
Xiaomin Zhang, Guangfeng Gu, Xuanfei Li, Chaopei Zhan. Lidocaine alleviates cisplatin resistance and inhibits migration of MGC-803/DDP cells through decreasing miR-10b. Cell cycle (Georgetown, Tex.). vol 19. issue 19. 2021-11-17. PMID:32892697. cell viability, apoptosis, migration and invasion were tested by cell counting kit-8 (cck-8) assay, apoptosis assay, western blot, migration and invasion assay. 2021-11-17 2023-08-13 Not clear
Ganghua Yang, Lijing Zhou, Qinhong Xu, Fandi Meng, Yong Wan, Xiankui Meng, Lin Wang, Lei Zhan. LncRNA KCNQ1OT1 inhibits the radiosensitivity and promotes the tumorigenesis of hepatocellular carcinoma via the miR-146a-5p/ACER3 axis. Cell cycle (Georgetown, Tex.). vol 19. issue 19. 2021-11-17. PMID:32936716. the protein levels of apoptosis markers, metastasis markers and acer3 were detected by western blot (wb) analysis. 2021-11-17 2023-08-13 Not clear
Ourlad Alzeus G Tantengco, Joy Vink, Paul Mark B Medina, Ramkumar Meno. Oxidative stress promotes cellular damages in the cervix: implications for normal and pathologic cervical function in human pregnancy†. Biology of reproduction. vol 105. issue 1. 2021-11-17. PMID:33760067. we treated the ectocervical and endocervical epithelial cells and cervical stromal cells with cigarette smoke extract, an oxidative stress inducer, for 48 h. cell viability (crystal violet assay); cell cycle, apoptosis, and necrosis (flow cytometry); senescence (senescence-associated β-galactosidase staining); autophagy (staining for autophagosome protein, microtubule-associated protein 1 light chain 3b); stress signaler p38 mitogen-activated protein kinases pathway activation (western blot analyses); and inflammation by measuring interleukin-6 (enzyme-linked immunosorbent assay) were conducted after 48 h of cigarette smoke extract treatment. 2021-11-17 2023-08-13 human
Xindong Liu, Xin Wang, Lijuan Zhang, Yi Zhou, Le Yang, Minghao Yan. By targeting apoptosis facilitator BCL2L13, microRNA miR-484 alleviates cerebral ischemia/reperfusion injury-induced neuronal apoptosis in mice. Bioengineered. vol 12. issue 1. 2021-11-16. PMID:33724167. then, cck8 assay western blot assay, and flow cytometry assay verified that upregulation of mir-484 increased viability and inhibited apoptosis of neurons following ogd. 2021-11-16 2023-08-13 mouse
Nianchun Hu, Ji-An Duan, Yuqin Yu, Dapeng Li, Jingli Chen, Hong Ya. Sevoflurane inhibits the migration, invasion and induces apoptosis by regulating the expression of WNT1 via miR-637 in colorectal cancer. Anti-cancer drugs. vol 32. issue 5. 2021-11-16. PMID:33735116. cell migration, invasion and apoptosis were detected by transwell assay, flow cytometry or western blot, respectively. 2021-11-16 2023-08-13 Not clear
Jie Zhang, Weichao Wang, Hong Wang, Junru Liu, Zhihong Zhan. Fasudil protects retinal ganglion cells and promotes axonal regeneration. Pakistan journal of pharmaceutical sciences. vol 33. issue 5(Special). 2021-11-16. PMID:33832886. apoptosis was detected by tunel, and surviving rgcs was detected by fluoro-gold retrograde label and hematoxylin-eosin (he) staining was used to evaluate pathological changes and western blot was used to measure the expression of protein. 2021-11-16 2023-08-13 rat