| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xin Xie, Hua Yang, Wenjun Yang, Tingshu Jian. Euphorbia angustifolia lactone B inhibits A549 proliferation and induces apoptosis. Pakistan journal of pharmaceutical sciences. vol 34. issue 6(Special). 2022-01-18. PMID:35039251. |
the content of cytochrome c (cyt c) protein and the expression quantity of bcl-2, bax, p-erk1/2, and p-akt proteins were detected by western blot and the apoptosis rates were detected by flow cytometry. |
2022-01-18 |
2023-08-13 |
Not clear |
| Yongtao Li, Wenshan Bian, Yang Jiang, Danyang Liu, Lei She. hMSCs Migrate under the Chemotaxis of CXCL-13 and Enhance Islet B Cell Activity through p-AKT Signaling Pathway in High-Glucose Environment. Journal of healthcare engineering. vol 2022. 2022-01-17. PMID:35035844. |
after coculture of hmscs and islet b cells, the activity of cells was detected by cck8 assay, the expression of ki-67 in cells was detected by rt-pcr, and the expression of p53 was detected by western blot to investigate the effect of hmscs on the proliferation and apoptosis of islet b cells. |
2022-01-17 |
2023-08-13 |
human |
| Yujie Xu, Dingmao Wang, Guodong Zha. Transcriptional activation of Proteasome 26S non-ATPase subunit 7 by forkhead box P3 participates in gastric cancer cell proliferation and apoptosis. Bioengineered. vol 13. issue 2. 2022-01-17. PMID:35037550. |
proliferation and apoptosis markers were assayed by qpcr and western blot. |
2022-01-17 |
2023-08-13 |
Not clear |
| Yixia Zhang, Xindan Meng, Keyu Li. The modulation of cAMP/PKA pathway by asiaticoside ameliorates high glucose-induced inflammation and apoptosis of retinal pigment epithelial cells. Journal of bioenergetics and biomembranes. 2022-01-17. PMID:35038080. |
then, cell survival rate, expression of inflammatory factors, oxidative stress, and apoptosis were measured by mtt method, western blot, oxidative stress detection kits and tunel respectively. |
2022-01-17 |
2023-08-13 |
human |
| Yong-Peng Wang, Fei Pan, Yu-Dan Wang, Afsar Khan, Ya-Ping Liu, Mei-Lian Yang, Jian-Xin Cao, Tian-Rui Zhao, Gui-Guang Chen. Anti-leukemic effect and molecular mechanism of 11-methoxytabersonine from Melodinus cochinchinensis via network pharmacology, ROS-mediated mitochondrial dysfunction and PI3K/Akt signaling pathway. Bioorganic chemistry. vol 120. 2022-01-16. PMID:35033818. |
western blot analysis showed that it can induce molt-4 cell apoptosis by up-regulating pi3k/akt signaling pathway. |
2022-01-16 |
2023-08-13 |
human |
| Lijie Ma, Chengfei Zhang, Lili Wu, Lingling Qin, Tonghua Li. Diosgenin reduces phosphodiesterase 3B (PDE3B) through AMP-activated protein kinase/ mechanistic target of rapamycin (AMPK/mTOR) signaling pathway to ameliorate streptozotocin-induced pancreatic β-cell apoptosis and dysfunction. Bioengineered. vol 13. issue 2. 2022-01-15. PMID:35030973. |
cell viability was detected by cck-8, apoptosis by flow cytometry, and apoptosis-related protein expression by western blot. |
2022-01-15 |
2023-08-13 |
rat |
| Chandramouli Ghosh, Aditi Nandi, Sudipta Bas. Lipid Nanoparticle-Mediated Induction of Endoplasmic Reticulum Stress in Cancer Cells. ACS applied bio materials. vol 2. issue 9. 2022-01-13. PMID:35021332. |
western blot analysis, fluorescent microscopy, and flow cytometry studies confirmed that 17aag-er-nps can concurrently activate er stress and nuclear dna impairment for arresting the cell cycle in the g2-m phase to elicit late apoptosis, followed by cell death, in a greatly augmented manner compared to free drugs. |
2022-01-13 |
2023-08-13 |
Not clear |
| H C Yang, Z K Xing, H Shao, X W Tan, E Q Wang, Y Liao, H J Chen, X W Wu, X L Chen, S J Zhan. The expression of cytokeratin and apoptosis-related molecules in echinococcosis related liver injury. Molecular and biochemical parasitology. 2022-01-12. PMID:35016896. |
cell counting kit-8 was used to detect cell proliferation, flow cytometry was used to detect hepatocyte apoptosis, and western blot was used to quantify cytokeratin and apoptosis-related proteins, such as caspase3, caspase9, bcl-2, and bax. |
2022-01-12 |
2023-08-13 |
mouse |
| Heung Man Lee, Shao Chin Lee, Lan He, Alice Pik Shan Kong, Dandan Mao, Yong Hou, Arthur Chi Kong Chung, Gang Xu, Ronald Ching Wan Ma, Juliana Chung Ngor Cha. Legacy effect of high glucose on promoting survival of HCT116 colorectal cancer cells by reducing endoplasmic reticulum stress response. American journal of cancer research. vol 11. issue 12. 2022-01-12. PMID:35018239. |
western blot analysis showed activation of caspase-3 in thapsigargin-treated cells in both normal and high glucose medium, albeit with lower levels of cleaved caspase-3 in cells exposed to high glucose, suggesting reduced apoptosis. |
2022-01-12 |
2023-08-13 |
Not clear |
| Lekshmi Krishna, Sagar Nilawar, Murugeswari Ponnalagu, Murali Subramani, Chaitra Jayadev, Rohit Shetty, Kaushik Chatterjee, Debashish Da. Fiber Diameter Differentially Regulates Function of Retinal Pigment and Corneal Epithelial Cells on Nanofibrous Tissue Scaffolds. ACS applied bio materials. vol 3. issue 2. 2022-01-12. PMID:35019286. |
the molecular changes in the cells in response to the different fibers were analyzed by qrt-pcr, western blot, immunofluorescence, elisa, flow cytometry, mtt assay, and sem to assess properties such as proliferation, apoptosis, membrane potential, epithelial-mesenchymal transition, stem cell population, vegf-a secretion, differentiation, and metabolic status of the cells. |
2022-01-12 |
2023-08-13 |
human |
| Changyu Zhu, Xiaolei Jiang, Hua Xiao, Jianmei Gua. Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery. Cell death discovery. vol 8. issue 1. 2022-01-11. PMID:35013115. |
moreover, cell viability was detected by means of mts, cell proliferation with a colony formation assay, cell apoptosis with flow cytometry, and expression patterns of related genes with the help of western blot analysis. |
2022-01-11 |
2023-08-13 |
mouse |
| Mahsa Toolabi, Fatemeh Safari, Adileh Ayati, Parnian Fathi, Setareh Moghimi, Somayeh Salarinejad, Roham Foroumadi, Shima H M E Ketabforoosh, Alireza Foroumad. Synthesis of novel 2-acetamide-5-phenylthio-1,3,4-thiadiazole-containing phenyl urea derivatives as potential VEGFR-2 inhibitors. Archiv der Pharmazie. 2022-01-11. PMID:35014090. |
further assessment by western blot analysis assay confirmed the induction of apoptosis by compound 9e, with upregulation of bax and downregulation of bcl-2 proteins in a431 cancer cells. |
2022-01-11 |
2023-08-13 |
human |
| Cuifeng Xia, Qiang Li, Xianshuo Cheng, Tao Wu, Pin Gao, Yongfang G. Insulin-like growth factor 2 mRNA-binding protein 2-stabilized long non-coding RNA Taurine up-regulated gene 1 (TUG1) promotes cisplatin-resistance of colorectal cancer via modulating autophagy. Bioengineered. vol 13. issue 2. 2022-01-11. PMID:35014946. |
flow cytometry examined apoptosis, transwell tracked migration and invasion, and western blot ascertained the protein profiles of autophagy proteins (lc3i/lc3ii and beclin1) and the hdgf/ddx5/β-catenin pathway. |
2022-01-11 |
2023-08-13 |
Not clear |
| Youhua Chen, Yan Mei, Lu Yang, Weibin Li, Yu Zhou, Surong He, Jie Lian. Taxifolin improves inflammatory injury of human bronchial epithelial cells by inhibiting matrix metalloproteinase (MMP) 10 via Wnt/β-catenin pathway. Bioengineered. vol 13. issue 1. 2022-01-10. PMID:35000533. |
then, light transmittance and apoptosis were then measured employing fluorescein transmittance, tunel and western blot. |
2022-01-10 |
2023-08-13 |
human |
| Weiqiang Xu, Fang Fang, Yuexuan Wang, Lijing Qin, Yu Han, Yuwei Huang, Bin Li, Yang Liu, Zhicheng Wan. Co-overexpression of TRAIL and Smac sensitizes MDA-MB-231 cells to radiation through apoptosis depending on mitochondrial pathway. Radiation and environmental biophysics. 2022-01-10. PMID:35006369. |
to explore the molecular mechanisms of apoptosis, mrna and protein levels of trail, smac, cytochrome c (cyt c), death receptor 5 (dr5), caspase-8, -9 and -3 were measured by quantitative real-time pcr, elisa and western blot, and caspase-3 activity was detected using caspase-3 activity kits. |
2022-01-10 |
2023-08-13 |
Not clear |
| Nadeeya Mad-Adam, Thidarath Rattanaburee, Tanotnon Tanawattanasuntorn, Potchanapond Graidis. Effects of trans-(±)-kusunokinin on chemosensitive and chemoresistant ovarian cancer cells. Oncology letters. vol 23. issue 2. 2022-01-07. PMID:34992691. |
moreover, western blot analysis was performed to determine the proteins level of topoisomerase ii, cyclin d1, cdk1, bax and p53-upregulated modulator of apoptosis (puma). |
2022-01-07 |
2023-08-13 |
Not clear |
| Xin-Yu Lu, Zhong-Ze Wang, Si-Cheng Wan, Er-Hu Zhao, Hong-Juan Cu. [Effects of tetrandrine on proliferation, migration, and invasion of glioblastoma cells]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 24. 2022-01-07. PMID:34994145. |
in this study, mtt and brdu assays were employed to detect the effect of tet on the proliferation of ln229 glioblastoma cells; flow cytometry was used to analyze the cycle distribution and apoptosis; plate cloning assay and soft agar colony formation assay were performed to study the colony formation ability of ln229 cells exposed to tet; scratch assay and transwell assay were conducted to detect the ability of migration and invasion; western blot was adopted to the exploration of the molecular mechanism. |
2022-01-07 |
2023-08-13 |
Not clear |
| Xin-Yu Lu, Zhong-Ze Wang, Si-Cheng Wan, Er-Hu Zhao, Hong-Juan Cu. [Effects of tetrandrine on proliferation, migration, and invasion of glioblastoma cells]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 24. 2022-01-07. PMID:34994145. |
the plate cloning assay and soft agar colony formation assay showed that tet weakened the colony formation of ln229 cells in vitro; cytometry assay showed that tet blocked cells in the g_1 phase and promoted cell apoptosis; scratch and transwell assays proved that tet inhibited the migration and invasion of ln229 cells; western blot results showed that tet down-regulated the expression levels of cdk2, cdk6, cyclin d1, cyclin e1, snail, slug, vimentin, and n-cadherin, while up-regulated the level of e-cadherin. |
2022-01-07 |
2023-08-13 |
Not clear |
| Chuanli Zeng, Gang Yuan, Yaoren Hu, Donghui Wang, Xiaojun Shi, Dedong Zhu, Airong Hu, Yina Meng, Jialin L. Repressing phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma by microRNA-142-3p restrains the progression of hepatocellular carcinoma. Bioengineered. vol 13. issue 1. 2022-01-06. PMID:34986757. |
for measuring apoptosis, flow cytometry and western blot were implemented. |
2022-01-06 |
2023-08-13 |
mouse |
| Hongbin Li, Nan Wang, Yuntian Xu, Xiao Chang, Jing Ke, Jun Yi. Upregulating microRNA-373-3p promotes apoptosis and inhibits metastasis of hepatocellular carcinoma cells. Bioengineered. vol 13. issue 1. 2022-01-05. PMID:34983307. |
flow cytometry and western blot analyzed cell apoptosis. |
2022-01-05 |
2023-08-13 |
mouse |