| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xin Zhang, Xuebing Zhou, Ming Gao, You Lyu, Ying Wang, Chunyu Yang, Yingshi Piao, Xiangshan Re. [Cordycepin inhibits the proliferation and migration of human gastric cancer cells by suppressing lipid metabolism via AMPK and MAPK activation]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 38. issue 6. 2022-06-22. PMID:35732609. |
the proliferation ability of mgc-803 and hgc-27 cells was detected by mtt assay and edu assay; the colony formation ability of cells was detected by colony formation assay; both wound healing assay and cell migration assay were applied to detect the cell migration ability of mgc-803 and hgc-27 cells; the chromatin agglutination was detected by hoechst 33342 staining; the apoptosis of gastric cancer cells was detected by annexin v-fitc/pi double labeling combined with flow cytometry; western blot was used to measure the protein expression levels of lipid metabolism-related proteins including sterol regulatory element binding transcription factor 1 (srebf1), fatty acid synthase (fasn), and acetyl coa carboxylase 1 (acc1), epithelial-mesenchymal transition (emt)-related proteins including e-cadherin, vimentin, snail, slug, matrix metalloproteinase 2 (mmp2), mmp9, ampk, and phosphorylated ampk (p-ampk), mapk signaling pathway-related proteins including jnk, phosphorylated jnk (p-jnk), p38 mapk, and p-p38 mapk, and apoptosis-related proteins including cleaved caspase-9 (c-caspase-9), c-caspase-3, and cleaved poly (adp-ribose) polymerase (c-parp). |
2022-06-22 |
2023-08-14 |
human |
| Yankui Huang, Qingxiang Cai, Huihui Liu, Yong Wang, Wuhua M. Remifentanil inhibits the inflammatory response of BV2 microglia and protects PC12 cells from damage caused by microglia activation. Bioengineered. vol 13. issue 5. 2022-06-21. PMID:35726401. |
in addition, tunel assay and western blot were performed to assess cell apoptosis. |
2022-06-21 |
2023-08-14 |
Not clear |
| Yanghui Huang, Guangyu Zhen. Circ_UBE2D2 Attenuates the Progression of Septic Acute Kidney Injury in Rats by Targeting miR-370-3p/NR4A3 Axis. Journal of microbiology and biotechnology. vol 32. issue 6. 2022-06-20. PMID:35722711. |
after sh-circ_ube2d2 was transfected into cells, the transfection efficiency was detected by qrt-pcr, cell viability and apoptosis were determined by mtt assay and flow cytometry, and expressions of bcl-2, bax and cleaved-caspase 3 were quantified by western blot. |
2022-06-20 |
2023-08-14 |
rat |
| Huaxiang Shi, Pengfei Li, Hu Zhou, Zhiyong Nie, Jingxin Zhang, Xin Sui, Jiabin Guo, Yongan Wang, Liyun Wan. Dynamic cerebral blood flow changes with FOXOs stimulation are involved in neuronal damage associated with high-altitude cerebral edema in mice. Brain research. 2022-06-20. PMID:35724762. |
similarly, western blot assays revealed upregulation of foxo1a, foxo3a, caspase-3 and bax, and downregulation of bcl-2, indicating a temporal influence of ahh on mitochondrial function and neuronal apoptosis. |
2022-06-20 |
2023-08-14 |
mouse |
| Guang-Chen Zhang, Yi-Hao Wang, Pan-Pan Ruan, Zu-Qi Zhang, Ning-Ning Shen, Yu-Fu Liu, Yu-Guang Wang, Yue Ga. [Protective effect of ophiopogonin D against isoproterenol-induced cardiomyocyte injury and targets]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 10. 2022-06-19. PMID:35718492. |
cell count kit-8(cck-8) assay was used to detect viability of cells treated with opd and iso, western blot to examine the effect of opd and iso on the expression of endoplasmic reticulum stress-related bip, bax, perk, atf4, caspase-12, and chop, flow cytometry to determine cell apoptosis rate, and hoechst 33258 and tunel staining to observe cell apoptosis and morphological changes. |
2022-06-19 |
2023-08-14 |
rat |
| Jingsong Liu, Xueming Zhang, Shengnan Yue, Jingcheng Fu, Jin Chen, Ruochao Huang, Pengyuan Shang, Kai Zhong, Shuang Guo, Xianqin Jiao, Guangming Zha, Liqiang Han, Guoyu Yang, Heping Li, Yueying Wan. The anti-apoptotic and anti-autophagic effects of EPO through PI3K/Akt/mTOR signaling pathway in MAC-T cells. Research in veterinary science. vol 149. 2022-06-17. PMID:35714559. |
western blot, rt-pcr, transmission electron microscope analysis and annexin v-fitc/pi were used to evaluate the regulation of epo on apoptosis and autophagy in inflammatory mac-t cells. |
2022-06-17 |
2023-08-14 |
cattle |
| Zhiguo Wu, Jian Tan, Lichang Lin, Wenting Zhang, Wanqiu Yua. microRNA-140-3p protects hippocampal neuron against pyroptosis to attenuate sevoflurane inhalation-induced post-operative cognitive dysfunction in rats via activation of HTR2A/ERK/Nrf2 axis by targeting DNMT1. Cell death discovery. vol 8. issue 1. 2022-06-16. PMID:35710537. |
in neurons, cell viability, apoptosis, and pyroptosis-related factors were tested by mtt, flow cytometry, and western blot analysis respectively. |
2022-06-16 |
2023-08-14 |
rat |
| Lillian Dong, Kevin Ke, Samina Badar, Ahmed H Mekkawy, Javed Akhter, Krishna Pillai, Carly J Carter, David L Morri. A novel method for potentiation of chemotherapy in soft tissue sarcomas with BromAc. American journal of translational research. vol 14. issue 5. 2022-06-15. PMID:35702130. |
cell proliferation, alongside western blot for a variety of cell cycle, apoptosis, and autophagy biomarkers assays was performed following treatment of cell lines in vitro at various concentrations of bromac and doxorubicin. |
2022-06-15 |
2023-08-14 |
Not clear |
| Jiefu Zhu, Yafei Zhang, Lang Shi, Yao Xia, Hongchu Zha, Huimin Li, Zhixia Son. RP105 protects against ischemic and septic acute kidney injury via suppressing TLR4/NF-κB signaling pathways. International immunopharmacology. vol 109. 2022-06-13. PMID:35696803. |
the tunel assay was used to detect apoptosis induced changes in the expression of rp105, and nuclear factor κb (nf-κb) in renal tissue detected by western blot. |
2022-06-13 |
2023-08-14 |
Not clear |
| Ying-Xian Liu, Yan-Jun Song, Xiao-Hang Liu, Si-Chi Xu, Chang Kong, Lian-Feng Chen, Hao Qian, Wei W. PD-1 inhibitor induces myocarditis by reducing regulatory T cells, activating inflammatory responses, promoting myocardial apoptosis and autophagy. Cytokine. vol 157. 2022-06-12. PMID:35691121. |
the expression level of pd-1 and regulatory t cells (tregs), cd4, cd8 + t cells, inflammation, apoptosis and autophagy-related factors, including il-6, tgf-β, ama-m2, fas/fasl, lc3 and p62 were detected in peripheral blood, muscle or myocardium of mice in each group, using elisa, rt-pcr, western blot and immunofluorescence. |
2022-06-12 |
2023-08-14 |
mouse |
| Peng Tu, Sujuan Yan, Fengping Zhan. Circ_0005925 Promotes Granulosa Cell Growth by Targeting MiR-324-3p to Upregulate MAP2K6 in Polycystic Ovary Syndrome. Biochemical genetics. 2022-06-11. PMID:35689709. |
western blot analysis was used to detect the protein expression of apoptosis markers and map2k6. |
2022-06-11 |
2023-08-14 |
Not clear |
| Xi Chen, Lei Zhang, JingBo Geng, Zhong Chen, XiaoPeng Cu. MiR-205-5p Functions as a Tumor Suppressor in Gastric Cancer Cells through Downregulating FAM84B. Journal of oncology. vol 2022. 2022-06-07. PMID:35669244. |
besides, the role of mir-205-5p in cell proliferation, cell apoptosis, cell cycle, cell invasion, and metastasis was assessed through cck-8 assay, colony formation, flow cytometry, scratch assay, transwell, and western blot. |
2022-06-07 |
2023-08-14 |
Not clear |
| Yuanyong Wang, Zhaoyang Wang, Changjian Shao, Guofang Lu, Mei Xie, Jian Wang, Hongtao Duan, Xiaofei Li, Wanpeng Yu, Weixun Duan, Xiaolong Ya. Melatonin may suppress lung adenocarcinoma progression via regulation of the circular non-coding RNA hsa_circ_0017109/miR-135b-3p/TOX3 axis. Journal of pineal research. 2022-06-06. PMID:35661247. |
also, cell apoptosis was detected using a cell apoptosis kit, and protein production was identified by western blot. |
2022-06-06 |
2023-08-14 |
human |
| Mersedeh Shayan, Soghra Mehri, Bibi Marjan Razavi, Hossein Hosseinzade. Minocycline Protects PC12 Cells Against Cadmium-Induced Neurotoxicity by Modulating Apoptosis. Biological trace element research. 2022-06-06. PMID:35661325. |
then, cd-induced apoptosis and mino anti-apoptotic effect were evaluated in both intrinsic and extrinsic pathways using western blot analysis. |
2022-06-06 |
2023-08-14 |
Not clear |
| Mengjie Zhang, Wanxuan Shao, Tongren Yang, Houli Liu, Shuai Guo, Deyao Zhao, Yuhua Weng, Xing-Jie Liang, Yuanyu Huan. Conscription of Immune Cells by Light-Activatable Silencing NK-Derived Exosome (LASNEO) for Synergetic Tumor Eradication. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2022-06-06. PMID:35665496. |
profiling of genes involved in apoptosis pathway with western blot and rna-seq in cells receiving neo treatment reveals that neo elicits effective nk cell-like cytotoxicity toward tumor cells. |
2022-06-06 |
2023-08-14 |
Not clear |
| Kexin Sun, Xu Wang, Xinyu Zhang, Xu Shi, Duqiang Gon. The antagonistic effect of melatonin on TBBPA-induced apoptosis and necroptosis via PTEN/PI3K/AKT signaling pathway in swine testis cells. Environmental toxicology. 2022-06-06. PMID:35665993. |
to investigate the cytotoxic of tbbpa on swine testis cells (st cells), as well as the antagonistic effect of mt, we established tbbpa exposure and mt antagonistic models, used flow cytometry and ao/eb staining methods to detect apoptosis and necroptosis, used dcfh-da method to examine the content of reactive oxygen species (ros) and investigated the expression of associated genes using rt-pcr and western blot. |
2022-06-06 |
2023-08-14 |
human |
| Zi Huang, Pei-Yao Xiao, Jing-Yan Chen, Qing Zeng, Bei-Xu Huang, Jian Yu, Song-Jie Lia. Mammalian Sterile 20-Like Kinase 1 Mediates Neuropathic Pain Associated with Its Effects on Regulating Mitophagy in Schwann Cells. Oxidative medicine and cellular longevity. vol 2022. 2022-06-03. PMID:35656021. |
ros level, mitochondria membrane potential, and apoptosis were assessed in vitro via flow cytometry and western blot. |
2022-06-03 |
2023-08-14 |
rat |
| Limei Wang, Dongjie Yin, Yanhui Fan, Ting Min, Yang Yi, Hongxun Wan. Molecular mechanism of the anti-gastric cancer activity of 1,2,3,6-tetra-O-galloyl-β-D-glucose isolated from Trapa bispinosa Roxb. shell in vitro. PloS one. vol 17. issue 6. 2022-06-02. PMID:35653387. |
the results of qpcr and western blot showed that 1,2,3,6-tetra-o-galloyl-β-d-glucose could induce apoptosis of sgc7901 cells by up-regulating the expression levels of p21, puma, perp and igf-bp3 genes, down-regulating the cyclind gene, increasing the expression levels of cytochrome c, caspase-3, caspase-9 protein and decreasing that of the protein bcl-2. |
2022-06-02 |
2023-08-14 |
Not clear |
| Eva Krishna Sutedja, Daniar Amarassaphira, Hanna Goenawan, Yuni Susanti Pratiwi, Nova Sylviana, Budi Setiabudiawan, Oki Suwarsa, Raden Tina Dewi Judistiani, Unang Supratman, Ronny Lesman. Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16-F10 Cells. Medical science monitor basic research. vol 28. 2022-06-01. PMID:35642437. |
material and methods in the present study, by utilizing b16-f10 cells, which is a melanoma cell line, we explored the anti-proliferative effect of calcitriol using cell viability assay, near-infrared imaging, expression of apoptosis-related genes using real-time polymerase chain reactions (pcr), and the expression of apoptosis proteins levels using western blot. |
2022-06-01 |
2023-08-13 |
Not clear |
| Beibei Qu, Xiuhua Han, Lan Zhao, Feifei Zhang, Qingmei Ga. Relationship of HIF‑1α expression with apoptosis and cell cycle in bone marrow mesenchymal stem cells from patients with myelodysplastic syndrome. Molecular medicine reports. vol 26. issue 1. 2022-06-01. PMID:35642674. |
reverse transcription‑quantitative pcr and western blot analyses were used to measure hif‑1α expression in cells from the two groups and apoptosis and cell cycle were also analyzed and compared between the groups using flow cytometry assays. |
2022-06-01 |
2023-08-13 |
human |