| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Chen Cui, Nan Zang, Jia Song, Xinghong Guo, Qin He, Huiqing Hu, Mengmeng Yang, Yuanqi Wang, Jingwen Yang, Ying Zou, Jing Gao, Lingshu Wang, Chuan Wang, Fuqiang Liu, Falian He, Xinguo Hou, Li Che. Exosomes derived from mesenchymal stem cells attenuate diabetic kidney disease by inhibiting cell apoptosis and epithelial-to-mesenchymal transition via miR-424-5p. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 36. issue 10. 2022-08-29. PMID:36036527. |
apoptosis was detected by western blot, tunel staining, and flow cytometry. |
2022-08-29 |
2023-08-14 |
mouse |
| Zhuo-Yao Li, Ai-Fang Zhou, Gan Li, Long-Yun Zhou, Pei-Min Pu, Ke Zhu, Zhong Zheng, Yong-Jun Wang, Qian-Qian Liang, Min Yao, Xue-Jun Cu. Chronic spinal cord compression associated with intervertebral disc degeneration in SPARC-null mice. Neural regeneration research. vol 18. issue 3. 2022-08-26. PMID:36018188. |
immunofluorescence staining and western blot assay showed that the absence of sparc induced apoptosis of neurons and oligodendrocytes, activation of microglia/macrophages with m1/m2 phenotype and astrocytes with a1/a2 phenotype; it also activated the expression of the nod-like receptor protein 3 inflammasome and inhibited brain-derived neurotrophic factor/tyrosine kinase b signaling pathway. |
2022-08-26 |
2023-08-14 |
mouse |
| Jirakhamon Sengking, Chio Oka, Nuttapong Yawoot, Jiraporn Tocharus, Waraluck Chaichompoo, Apichart Suksamrarn, Chainarong Tocharu. Protective Effect of Neferine in Permanent Cerebral Ischemic Rats via Anti-Oxidative and Anti-Apoptotic Mechanisms. Neurotoxicity research. 2022-08-26. PMID:36018507. |
in addition, the brain tissues were examined by western blot analysis to calculate the expression of proteins related to oxidative stress and apoptosis. |
2022-08-26 |
2023-08-14 |
rat |
| Maonan Wang, Bo Xu, Lintao Liu, Dawei Wan. Oridonin attenuates dextran sulfate sodium‑induced ulcerative colitis in mice via the Sirt1/NF‑κB/p53 pathway. Molecular medicine reports. vol 26. issue 4. 2022-08-25. PMID:36004485. |
tunel and western blot assays were also performed to detect cell apoptosis, and the expression level of sirt1/nf‑κb/p53 pathway‑related proteins was also determined using western blot analysis. |
2022-08-25 |
2023-08-14 |
mouse |
| Yan Xu, Ji Zheng, Zhi-Ying Y. Anlotinib inhibits the proliferation and induces apoptosis by inactivating the AKT pathway in androgen receptor-negative prostate cancer cells. Neoplasma. 2022-08-25. PMID:36004650. |
cell nuclear morphology was observed after dapi staining, cell apoptosis level was evaluated by annexin-v-fitc/pi staining, and western blot was used to detect the proliferation- and apoptosis-related proteins. |
2022-08-25 |
2023-08-14 |
Not clear |
| Abolfazl Shakeri, Toktam Hafezian, Norbert Kúsz, Judit Hohmann, Motahare Boozari, Javad Mottaghipisheh, Seyed Ahmad Emami, Zahra Tayarani-Najaran, Javad Asil. Cytotoxicity, apoptosis inducing activity and Western blot analysis of tanshinone derivatives from Stachys parviflora on prostate and breast cancer cells. Molecular biology reports. 2022-08-24. PMID:36002657. |
cytotoxicity, apoptosis inducing activity and western blot analysis of tanshinone derivatives from stachys parviflora on prostate and breast cancer cells. |
2022-08-24 |
2023-08-14 |
human |
| Ji Di, Bo Jiang, Hao Shang, Xin Yang, Chengwu Zhang, Xiali Shi, Yan Cha. Study on the Mechanism of Action of STAT3 in the Drug Resistance of Gastric Cancer Cells. Contrast media & molecular imaging. vol 2022. 2022-08-22. PMID:35992548. |
this research detected the expression of stat3 in gastric cancer tissues and adjacent tissues by western blot and immunohistochemical experiments and conducted corresponding basic experiments to explore the role of stat3 in inhibiting the proliferation of cisplatin-resistant gastric cancer cells and promoting their apoptosis, including the construction of cisplatin-resistant gastric cancer cell line, the knock-out stat3 in drug-resistant gastric cancer cells by crispr-cas9, and the comparison of the proliferation and apoptosis of drug-resistant cells and drug-resistant cells stat3(-). |
2022-08-22 |
2023-08-14 |
Not clear |
| Lifeng Wu, Na Zuo, Shuo Pan, Yue Wang, Qixue Wang, Jun M. miR-1246 Promotes Laryngeal Squamous Cell Carcinoma Progression by Interacting with THBS1. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer. vol 41. issue 3. 2022-08-22. PMID:35993956. |
in addition, the roles of mir-1246 in the proliferation, apoptosis and migration of tu212 and amc-hn-8 cells were measured by colony formation, flow cytometry, wound-healing, and western blot (wb) assays, respectively. |
2022-08-22 |
2023-08-14 |
Not clear |
| Ling Qiang Bai, Bin Zhe Wang, Qi Wei Liu, Wen Qiang Li, Hang Zhou, Xiao Yan Yan. Effects of penehyclidine hydrochloride on myocardial ischaemia-reperfusion injury in rats by inhibiting TLR4/MyD88/NF-κB pathway via miR-199a-3p. Growth factors (Chur, Switzerland). 2022-08-19. PMID:35984706. |
he staining was applied to detect pathological tissue structure, tunel staining to detect apoptosis rate, qrcr and western blot to detect mir-199a-3p and tlr4/myd88/nf-κb expressions in rat myocardial tissues. |
2022-08-19 |
2023-08-14 |
rat |
| Tian-Jiao Lyu, Zi-Xiu Zhang, Jun Chen, Zong-Jun Li. Ginsenoside Rg1 ameliorates apoptosis, senescence and oxidative stress in ox-LDL-induced vascular endothelial cells via the AMPK/SIRT3/p53 signaling pathway. Experimental and therapeutic medicine. vol 24. issue 3. 2022-08-18. PMID:35978936. |
cell counting kit-8 assay, tunel staining, western blot analysis of apoptosis-related proteins and senescence-related proteins, senescence-associated β-galactosidase staining, elisa and other techniques including related kits of oxidative stress markers were used to detect the viability, apoptosis, oxidative stress, inflammatory cytokines including il-1β, il-6 and tnf-α and senescence of ox-ldl-induced huvecs induced by rg1. |
2022-08-18 |
2023-08-14 |
human |
| Lijun Wang, Songqing Lai, Huaxi Zou, Xueliang Zhou, Qing Wan, Yong Luo, Qicai Wu, Li Wan, Jichun Liu, Huang Huan. Ischemic preconditioning/ischemic postconditioning alleviates anoxia/reoxygenation injury via the Notch1/Hes1/VDAC1 axis. Journal of biochemical and molecular toxicology. 2022-08-17. PMID:35975741. |
the expression of hes1 and vdac1 proteins were measured by western blot analysis, while the levels of reactive oxygen species (ros), mitochondrial membrane potential (ΔΨm), and apoptosis were evaluated by flow cytometry. |
2022-08-17 |
2023-08-14 |
Not clear |
| Huimin Li, Yunhan Zhao, Qiuzi Shen, Honggang L. Multiple circRNAs regulated by QKI5 conjointly spongemiR-214-3p to antagonize Bisphenol A-inducedspermatocyte toxicity. Acta biochimica et biophysica Sinica. 2022-08-12. PMID:35959880. |
here by cck-8 assay, apoptosis assay, qrt-pcr and western blot analysis, we further discover that circrnas (represented by circdcbld2, circmapk1 and circtbcld20) can cooperatively sponge mir-214-3p and then up-regulate akt1 in ameliorating bpa-induced reproductive toxicity. |
2022-08-12 |
2023-08-14 |
Not clear |
| Yun Ji, Yu He, Ying Yang, Zhaolai Dai, Zhenlong W. Hydroxyproline alleviates 4-hydroxy-2-nonenal-induced DNA damage and apoptosis in porcine intestinal epithelial cells. Animal nutrition (Zhongguo xu mu shou yi xue hui). vol 9. 2022-08-11. PMID:35949986. |
thereafter, the cells were subjected to apoptosis detection by hoechst staining, flow cytometry, and western blot or dna damage analysis by comet assay, immunofluorescence, and reverse-transcription quantitative pcr (rt-qpcr). |
2022-08-11 |
2023-08-14 |
Not clear |
| Dongfang Guo, Airong Zhang, Meifang Suo, Ping Wang, Yile Lian. ELK1-Induced upregulation of long non-coding TNK2-AS1 promotes the progression of acute myeloid leukemia by EZH2-mediated epigenetic silencing of CELF2. Cell cycle (Georgetown, Tex.). 2022-08-09. PMID:35941836. |
the changes of the proliferation, apoptosis, and differentiation in tnk2-as1 shrna-transfected hl-60 and thp-1 cells were detected with cck-8, edu, flow cytometry, western blot, and nbt assays. |
2022-08-09 |
2023-08-14 |
Not clear |
| Xu Zhao, Dongge Qiao, Dongsheng Guan, Kun Wang, Yinglin Cu. Chrysophanol Ameliorates Hemin-Induced Oxidative Stress and Endoplasmic Reticulum Stress by Regulating MicroRNA-320-5p/Wnt3a Pathway in HT22 Cells. Oxidative medicine and cellular longevity. vol 2022. 2022-08-08. PMID:35936221. |
the cell viability, apoptosis, er stress, and oxidative stress were evaluated by conducting the cell counting kit-8 (cck-8), tdt-mediated dutp nick end labeling (tunel) staining assays, western blot, and corresponding kit, respectively. |
2022-08-08 |
2023-08-14 |
Not clear |
| Fang Hao, Chunguang Shan, Yubo Zhang, Ying Zhang, Zhanwei Ji. Exosomes Derived from microRNA-21 Overexpressing Neural Progenitor Cells Prevent Hearing Loss from Ischemia-Reperfusion Injury in Mice via Inhibiting the Inflammatory Process in the Cochlea. ACS chemical neuroscience. 2022-08-08. PMID:35939349. |
immunofluorescence staining of caspase-3 and parvalbumin was used to detect apoptosis hair cells in the cochlea, and western blot was utilized to detect the relative expressions of p53 and inflammatory cytokines in the cochlea. |
2022-08-08 |
2023-08-14 |
mouse |
| Yu Xiong, Chuanbin Yang, Xiaobo Yang, Caihong Ding, Qing Wang, Huaiyang Zh. LncRNA MIR9-3HG enhances LIMK1 mRNA and protein levels to contribute to the carcinogenesis of lung squamous cell carcinoma via sponging miR-138-5p and recruiting TAF15. Pathology, research and practice. vol 237. 2022-08-07. PMID:35933883. |
functional assays encompassing colony formation, 5-ethynyl-2'-deoxyuridine (edu) staining, transwell and flow cytometry assays and western blot detecting related proteins demonstrated that mir9-3hg depletion hampered cell proliferation, migration, invasion and epithelial-mesenchymal transition (emt) while accelerating cell apoptosis in lusc. |
2022-08-07 |
2023-08-14 |
Not clear |
| Jasmine Bagge, Arvind Manikantan Padma, Anna Casselbrant, Mats Hellström, Mihai Oltea. MUCOSAL RECOVERY AFTER INTESTINAL TRANSPLANTATION IN THE RAT: A SEQUENTIAL HISTOLOGICAL AND MOLECULAR ASSESSMENT. European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes. 2022-08-04. PMID:35926441. |
histology studies (goblet cell count, morphometry), immunofluorescence and western blot for several tight junction proteins, apoptosis and inflammation related proteins were performed. |
2022-08-04 |
2023-08-14 |
rat |
| Z Junde, L Tingting, Z Lu, C Shan, Y Da. Lithium chloride promotes neural functional recovery after local cerebral ischemia injury in rats through Wnt signaling pathway activation. Folia morphologica. 2022-08-02. PMID:35916382. |
apoptosis of the hippocampal neurons was tested by western blot. |
2022-08-02 |
2023-08-14 |
human |
| Mingsheng Liu, Xiaomei Liu, Yuan Wang, Yutong Sui, Feilin Liu, Zinan Liu, Fei Zou, Kuiyang Zuo, Ziyu Wang, Wei Sun, Qi Xu, Dan Liu, Jinyu Li. Intrinsic ROS Drive Hair Follicle Cycle Progression by Modulating DNA Damage and Repair and Subsequently Hair Follicle Apoptosis and Macrophage Polarization. Oxidative medicine and cellular longevity. vol 2022. 2022-07-29. PMID:35903712. |
the results of western blot and histological staining indicated that aif-parp1 plays a key role in hf apoptosis, but their role in the regulation of the hf cycle is not clear. |
2022-07-29 |
2023-08-14 |
mouse |