| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Lijie Gong, Chen Chen, Xiaoqin Liu, Xiutao Wu, Ling Zhu, Jianguang Luo, Lingyi Kon. Hainanolide inhibits the progression of colon cancer via inducing the cell cycle arrest, cell apoptosis and activation of the MAPK signaling pathway. Toxicology and applied pharmacology. 2022-09-20. PMID:36126765. |
western blot indicated that hn promoted apoptosis by up-regulating bax and down-regulated bcl-2. |
2022-09-20 |
2023-08-14 |
mouse |
| Tao Ma, Yan Chen, Zhi-Gang Yi, Jia Liu, Yan-Hong Li, Jun Bai, Wen-Ting Tie, Mei Huang, Xiao-Feng Zhu, Ji Wang, Juan Du, Xiu-Qin Zuo, Qin Li, Fan-Li Lin, Liu Tang, Jing Guo, Hong-Wen Xiao, Qian Lei, Xiao-Li Ma, Li-Juan Li, Lian-Sheng Zhan. NORAD promotes multiple myeloma cell progression via BMP6/P-ERK1/2 axis. Cellular signalling. 2022-09-20. PMID:36126794. |
the norad gene was knockdown by lentiviral transfection in mm cell lines, and the effects of norad on apoptosis, cell cycle and cell proliferation in mm cells were examined by flow cytometry, cck8 assay, edu assay and western blot, and the differential genes after knockdown of norad were screened by mrna sequencing, followed by in vivo experiments and immunohistochemical assays. |
2022-09-20 |
2023-08-14 |
Not clear |
| Fei Zhu, Jianzhong Xiong, Fei Yi, Ermin Luo, Chun Huang, Runying L. Albiflorin relieves cerebral ischemia-reperfusion injury by activating Nrf2/HO-1 pathway. Histology and histopathology. 2022-09-19. PMID:36121178. |
flow cytometry, western blot, or tunel showed that af treatment reduced apoptosis of cells or rat brain tissues. |
2022-09-19 |
2023-08-14 |
rat |
| Chandrakant Bagul, Garikapati Koteswara Rao, Immadi Veena, Ravindra Kulkarni, Jaki R Tamboli, Ravikumar Akunuri, Siddiq Pasha Shaik, Manika Pal-Bhadra, Ahmed Kama. Benzimidazole-linked pyrazolo[1,5-a]pyrimidine conjugates: synthesis and detail evaluation as potential anticancer agents. Molecular diversity. 2022-09-17. PMID:36114912. |
western blot analysis demonstrated an elevated level of apoptotic proteins such as p53, p21, bax, whereas a decrease in the antiapoptotic protein bcl-2 and procaspase-9, confirming the ability of these conjugates to trigger cell death by apoptosis. |
2022-09-17 |
2023-08-14 |
Not clear |
| Shan Xu, Xin Sun, Leixuan Luo, Yang Yang, Qiuyan Guo, Sheng Tang, Zhiyan Jiang, Yuzhen Li, Jiaqian Han, Wenhui Gan, Feiyi Yang, Xuan Zhang, Yijun Liu, Chuanchuan Sun, Jie He, Meng Liu, Daiying Zuo, Wufu Zhu, Yingliang W. XS-2, a novel potent dual PI3K/mTOR inhibitor, exhibits high in vitro and in vivo anti-breast cancer activity and low toxicity with the potential to inhibit the invasion and migration of triple-negative breast cancer. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 155. 2022-09-16. PMID:36113258. |
in order to investigate the antitumor mechanism of xs-2, a series of experiments were carried out in vitro/in vivo animal model and molecular biological levels such as the cell cycle and the apoptosis assay, real-time pcr, western blot, docking and molecular simulations analysis, etc. |
2022-09-16 |
2023-08-14 |
mouse |
| Jinqing Huang, Lang Hong, Binghua Shen, Yunying Zhou, Jianyun Lan, Ying Pen. FOXO1 represses MCL1 transcription to regulate the function of vascular smooth muscle cells in intracranial aneurysm. Experimental brain research. 2022-09-14. PMID:36104628. |
after foxo1 and/or mcl1 were overexpressed in hbvsmcs, caspase-3 and cyt-c protein expression were examined by western blot, cell proliferation by cck-8 and edu assays, and cell apoptosis by flow cytometry. |
2022-09-14 |
2023-08-14 |
human |
| Nan Chen, Jie Deng, Zhihui Zhang, Xia Feng, Hongkang Wang, Jian Chen, Ling Li, Yemin Cao, Chenglin Jia, Yongbing Ca. Oxidative stress-triggered pyroptosis mediates Candida albicans susceptibility in diabetic foot. Microbial pathogenesis. 2022-09-10. PMID:36087690. |
notably, in this study, through the investigation of murine diabetic footpad c. albicans infection model and molecular biotechnology, including histopathological staining, immunofluorescence (if) staining, quantitative real-time pcr (qpcr), western blot (wb), flow cytometry (fcm), sandwich enzyme-linked immunosorbent assay (elisa) assays, we found that intense os responses in the footpad tissue not only mediated the activation of nf-κb protein complex, but also triggered downstream pyroptosis and apoptosis through nlrp3 inflammasome, which is one of the potential reasons for the severe condition of infectious skin injuries in diabetic mice. |
2022-09-10 |
2023-08-14 |
mouse |
| Xiaosu Huang, Pei Yan, Wenqing Ding, Chang Zhou, Qiuxiang Xu, Ming Li, Lianbao Ye, Weiqiang Che. α-Pinene inhibits the growth of cervical cancer cells through its proapoptotic activity by regulating the miR-34a-5p/Bcl-2 signaling axis. Drug development research. 2022-09-08. PMID:36074793. |
therefore, we investigated the effect of α-pinene on apoptosis in cc via in vitro assays of flow cytometry (fcw), terminal deoxynucleotidyl transferase-mediated nick end labeling (tunel) assay, quantitative real-time polymerase chain reaction (qrt-pcr), and western blot. |
2022-09-08 |
2023-08-14 |
Not clear |
| Jing Luo, Ning Jiang, Jialei Chen, Gao Yu, Jing Zhao, Changhong Yang, Yong Zha. Inhibition of miR-423-5p exerts neuroprotective effects in an experimental rat model of cerebral ischemia/reperfusion injury. Neuroscience. 2022-09-06. PMID:36067951. |
subsequently, western blot, elisa, mpo, tunel and commercial assay kits were applied to assess the influence of mir-423-5p on nlrp3 inflammasome, apoptosis, and oxidative stress levels in ischemic penumbra tissue. |
2022-09-06 |
2023-08-14 |
rat |
| Mei Yuan, Bozhi Yua. Antidepressant-like effects of Rehmannioside A on rats induced by chronic unpredictable mild stress through inhibition of endoplasmic reticulum stress and apoptosis of hippocampus. Journal of chemical neuroanatomy. 2022-09-06. PMID:36067970. |
the effect of rea on the neuronal protection, apoptosis and endoplasmic reticulum stress (ers) was evaluated by he, nissl, if and tunel staining, and western blot assays. |
2022-09-06 |
2023-08-14 |
rat |
| Jiefei Zhu, Yan Li, Xu L. IL4I1 enhances PD-L1 expression through JAK/STAT signaling pathway in lung adenocarcinoma. Immunogenetics. 2022-09-03. PMID:36056935. |
we carried out several methods to examine cell functions: cck-8 to measure luad cell proliferation; flow cytometry to determine cell apoptosis; western blot to assess the expression of jak/stat pathway-related proteins and pd-l1; t cell cytotoxicity assay to evaluate the effect of il4i1 on the immune escape of luad cells. |
2022-09-03 |
2023-08-14 |
Not clear |
| Jian Pan, Guoxiu Zhang, Yingying Hu, Hongwei Jiang, XianMing Tang, Dongshan Zhan. MiR-6918-5p prevents renal tubular cell apoptosis by targeting MBD2 in ischemia/reperfusion-induced AKI. Life sciences. 2022-09-03. PMID:36057400. |
cell apoptosis was detected by cck8, flow cytometry and western blot, while he staining and tunel staining were used to assess the degree of kidney damage. |
2022-09-03 |
2023-08-14 |
mouse |
| Huan Wang, Mei-Lin Liu, Chu Chu, Shi-Jiao Yu, Jing Li, Hai-Chuan Shen, Qian Meng, Teng Zhan. Paeonol alleviates placental inflammation and apoptosis in preeclampsia by inhibiting the JAK2/STAT3 signaling pathway. The Kaohsiung journal of medical sciences. 2022-09-02. PMID:36054229. |
western blot analysis was performed to detect the protein levels of apoptosis markers and janus kinase 2/signal transducer and activator of transcription 3 (jak2/stat3) signaling pathway-related molecules. |
2022-09-02 |
2023-08-14 |
mouse |
| Bo Li, Jie Wang, Ming Yuan, Yuchen Miao, Hui Zhang, Junjie Zhan. Design, Synthesis and Biological Evaluation of Tetrahydroisoquinoline Stilbene Derivatives as Potential Antitumor Candidates. Chemical biology & drug design. 2022-09-02. PMID:36054251. |
flow cytometry and western blot analysis indicated that 16e caused apoptosis via the mitochondrial-dependent apoptotic pathway by reducing mitochondrial membrane potential, inducing ros accumulation, promoting the release of cytochrome c from the mitochondria into the cytoplasm, and further increasing the protein level of cleaved caspase-3. |
2022-09-02 |
2023-08-14 |
human |
| Min Yu, Yan Li, Xianpei Tan, Qiao H. Steroid Receptor Coactivator-Interacting Protein (SIP) Alleviates Ischemic Cerebral Infarction Damage Through the NF-κB Pathway. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 2022-09-02. PMID:36055280. |
after ischemia/reperfusion, neurological function, neuroglia cells activation, infarction volume, brain water content, brain tissue apoptosis ratio, and inflammatory response were assessed, and quantitative pcr and western blot were also detected, respectively. |
2022-09-02 |
2023-08-14 |
Not clear |
| Mansoor-Ali Vaali-Mohammed, Maha-Hamadien Abdulla, Sabine Matou-Nasri, Wagdy M Eldehna, M Meeramaideen, Eslam B Elkaeed, Mohammed El-Watidy, Noura S Alhassan, Khayal Alkhaya, Omar Al Obee. The Anticancer Effects of the Pro-Apoptotic Benzofuran-Isatin Conjugate (5a) Are Associated With p53 Upregulation and Enhancement of Conventional Chemotherapeutic Drug Efficiency in Colorectal Cancer Cell Lines. Frontiers in pharmacology. vol 13. 2022-09-01. PMID:36046830. |
western blot and rt-qpcr were performed to assess the protein and transcript expression levels of epithelial-mesenchymal transition (emt), cell cycle, and apoptosis markers. |
2022-09-01 |
2023-08-14 |
Not clear |
| Xin Xiao, Wen-Hua Xu, Xiao-Qing Zhang, Jun-Feng Ding, Yue Jiang, Jun T. [Anti-oxidative and anti-apoptotic effects and molecular mechanisms of catalpol against H_2O_2-induced oxidative damage in pancreatic β cells (INS-1 cells)]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 16. 2022-09-01. PMID:36046869. |
the present study investigated the anti-oxidative and anti-apoptotic effects and molecular mechanisms of catalpol on the h_2o_2-induced pancreatic β-cells(ins-1 cells).the oxidative damage model of ins-1 cells was induced and optimized by the stimulation of h_2o_2 of different concentrations for different time.cck-8 assay was used to detect cell viability after catalpol intervention(1, 5, 10, 20, 40, 80, and 160 μmol·l~(-1)) for 24 h.intracellular reactive oxygen species(ros), superoxide dismutase(sod), and lipid peroxide malondialdehyde(mda) were measured by dcfh-da fluorescent probe, wst-1, and tba respectively.moreover, the apo-ptotic effect was detected by ao-eb and annexin v-fitc/pi staining.in addition, the protein expression levels were detected by wes-tern blot, and intracellular insulin concentration was measured by elisa.the results showed that the oxidative damage model of ins-1 cells was stably induced by 50 μmol·l~(-1) h_2o_2 treatment for 2 h, and catalpol at 1-80 μmol·l~(-1) did not affect cell viability of ins-1 cells.compared with the conditions in the model group, 1, 5, and 10 μmol·l~(-1) catalpol intervention for 2 h could protect ins-1 cells from oxidative damage(p<0.001), reduce ros and mda, increase sod, and inhibit excessive cell apoptosis.moreover, 1, 5, and 10 μmol·l~(-1) catalpol could also up-regulate the phosphorylation of nuclear transcription factor nf-e2 related factors, negatively regulate kelch-like ech-associated protein 1(keap1), phosphorylation of extracellular signal-regulated kinase(erk), and heme oxyge-nase 1(ho-1), and promote the protein expression of pancreatic-duodenal homeobox factor-1(pdx-1) and glucose transporter 2(glut2).in addition, 1, 5, and 10 μmol·l~(-1) catalpol increased insulin secretion of ins-1 cells under oxidative damage in the high-glucose culture medium, indicating function recovery of pancreatic β cells.pdx-1 is a key nuclear transcription factor of pancreatic β cell function that directly regulates glut2 and insulin synthesis, and affects glucose homeostasis.in conclusion, catalpol can reduce the oxidative damage and apoptosis of ins-1 cells, activate antioxidant pathway, protect the function of pancreatic β cells, and improve insulin synthesis and secretion. |
2022-09-01 |
2023-08-14 |
Not clear |
| Meng-Yu Zuo, Tong-Juan Tang, Peng Zhou, Xiang Wang, Rui Ding, Jin-Fan Gu, Jian Chen, Liang Wang, Juan Yao, Xiang-Yang Li, Jin-Ling Huan. [Mechanism of atractylenolide Ⅲ in alleviating H9c2 cell apoptosis through ROS/GRP78/caspase-12 signaling pathway based on molecular docking]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 16. 2022-09-01. PMID:36046873. |
this study aims to investigate the effect of atractylenolide Ⅲ(atl-Ⅲ) on hydrogen peroxide(h_2o_2)-induced endoplasmic reticulum stress and apoptosis of h9 c2 cells via the ros/grp78/caspase-12 signaling pathway.the binding activity of atl-Ⅲ to grp78 was determined by molecular docking.the result showed that atl-Ⅲ had a good binding activity to grp78, and the binding activity of atl-Ⅲ was stronger than that of its specific inhibitor.the endoplasmic reticulum stress model of h9 c2 was established by h_2o_2(100 μmol·l~(-1)) treatment.five groups were designed: blank control group, model group, and atl-Ⅲ(15, 30, and 60 μmol·l~(-1)) groups.apoptosis was detected by hoechst/pi double staining and flow cytometry.the levels of superoxide dismutase(sod), malondialdehyde(mda), and lactate dehydrogenase(ldh) were measured by colorimetry.the levels of reactive oxygen species(ros) and calcium(ca~(2+)) in cytoplasm were determined by the fluorescence probe dcfh-da and the calcium fluorescence probe flou-4, respectively.the protein levels of grp78, caspase-12, and caspase-3 were determined by western blot, and the mrna levels of grp78 and caspase-12 by rt-qpcr.n-acetyl-l-cysteine(nac) and 4-phenylbutyric acid(4-pba) were respectively used to inhibit ros and grp78, and then the mechanism of atl-Ⅲ in protecting the cells from endoplasmic reticulum stress induced by h_2o_2 were deduced.atl-Ⅲ(15, 30, and 60 μmol·l~(-1)) decreased the apoptosis rate and ros, mda, and ldh levels(p<0.01), increased the sod activity(p<0.01), and down-regulated the protein levels of grp78, caspase-12, and caspase-3 and the mrna levels of grp78 and caspase-12(p<0.05).the addition of nac decreased the apoptosis rate and ros, mda, grp78, caspase-12, and caspase-3 levels(p<0.01), while it elevated the sod level(p<0.01).the addition of 4-pba also decreased the apoptosis rate and the levels of grp78, caspase-12, caspase-3, and ca~(2+)(p<0.01).the effect of inhibitors were consistent with that of atl-Ⅲ.in conclusion, atl-Ⅲ can protect h9 c2 cardiomyocytes by regulating ros/grp78/caspase-12 signaling pathway to inhibit h_2o_2-induced endoplasmic reticulum stress and apoptosis. |
2022-09-01 |
2023-08-14 |
Not clear |
| Tong Zhang, Guo-Yan Liu, Jing-Long Cao, Yan-Nan Li, Hui Xue, Hai-Tao Wu, Cheng-Hao Ji. Peimine-induced apoptosis and inhibition of migration by regulating reactive oxygen species-mediated MAPK/STAT3/NF-κB and Wnt/β-catenin signaling pathways in gastric cancer MKN-45 cells. Drug development research. 2022-09-01. PMID:36048972. |
additionally, western blot was used to analyze the expression of signaling pathway proteins and the relationship between apoptosis and ros accumulation. |
2022-09-01 |
2023-08-14 |
Not clear |
| Sheau-Chung Tang, Jiunn-Liang Ko, Chun-Te Lu, Pui-Ying Leong, Chu-Chyn Ou, Chih-Ting Hsu, Yu-Ping Hsia. Chloroquine alleviates the heat-induced to injure via autophagy and apoptosis mechanisms in skin cell and mouse models. PloS one. vol 17. issue 8. 2022-08-31. PMID:36044415. |
western blot assays were performed to assess expression of proteins associated with autophagy, apoptosis, and endoplasmic reticulum stress in skin cells following burns. |
2022-08-31 |
2023-08-14 |
mouse |