| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Li-Fang Shi, Hong-Bing Ru. [Effects of DLL4 gene on YY1 and c-Myc protein expression and cell proliferation in leukemia cell line K562]. Zhongguo shi yan xue ye xue za zhi. vol 19. issue 6. 2012-11-29. PMID:22169291. |
at 48 hours after transfection, the expression level of dll4, yy1 and c-myc proteins in k562 cells of each group were detected by western blot and indirect immunocytochemical method; the cck-8 method was used to detect proliferation of k562 cells; at 48 hours after transfection, cell cycle distribution and apoptosis of k562 cells were detected by flow cytometry. |
2012-11-29 |
2023-08-12 |
Not clear |
| Jun Hong, Ahmed Katsha, Pengcheng Lu, Yu Shyr, Abbes Belkhiri, Wael El-Rifa. Regulation of ERBB2 receptor by t-DARPP mediates trastuzumab resistance in human esophageal adenocarcinoma. Cancer research. vol 72. issue 17. 2012-11-19. PMID:22745369. |
the annexin-v staining and western blot analysis indicated that t-darpp effectively abrogated trastuzumab-induced apoptosis, inhibited cleavage of caspase-3, and blocked trastuzumab-induced dephosphorylation of erbb2 and akt proteins. |
2012-11-19 |
2023-08-12 |
mouse |
| Xiao Wen Mao, James D Crapo, Daila S Gridle. Mitochondrial oxidative stress-induced apoptosis and radioprotection in proton-irradiated rat retina. Radiation research. vol 178. issue 3. 2012-11-16. PMID:22780102. |
proton irradiation was delivered to the same eye at doses of 1 or 4 gy and assays were done at 6 h. levels of bax, bcl-2 and sod2 proteins were evaluated by western blot and caspase-3 immunohistochemistry was performed to confirm the occurrence of apoptosis. |
2012-11-16 |
2023-08-12 |
rat |
| Guanghong Jia, Ryan M Stormont, Deepak M Gangahar, Devendra K Agrawa. Role of matrix Gla protein in angiotensin II-induced exacerbation of vascular calcification. American journal of physiology. Heart and circulatory physiology. vol 303. issue 5. 2012-11-07. PMID:22796540. |
the expression of mgp, calcification, and apoptosis in human vsmcs were examined by western blot analysis, real-time pcr, in situ terminal deoxynucleotidyltransferase-mediated dutp nick end labeling, and enzyme-linked immunosorbent assay, respectively. |
2012-11-07 |
2023-08-12 |
human |
| Ying Zhang, Meng Luo, Yuangang Zu, Yujie Fu, Chengbo Gu, Wei Wang, Liping Yao, Thomas Effert. Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway. Chemico-biological interactions. vol 199. issue 2. 2012-11-06. PMID:22796323. |
western blot results showed that dryofragin inhibited bcl-2 and induced bax expression which led to an activation of caspases-9 and -3 in the cytosol, and further cleavage of poly adp-ribose polymerase (parp) in the nucleus, then induced cell apoptosis. |
2012-11-06 |
2023-08-12 |
human |
| Darya Habibi, Nadya Ogloff, Reza B Jalili, Arla Yost, Andrew P Weng, Aziz Ghahary, Christopher J On. Borrelidin, a small molecule nitrile-containing macrolide inhibitor of threonyl-tRNA synthetase, is a potent inducer of apoptosis in acute lymphoblastic leukemia. Investigational new drugs. vol 30. issue 4. 2012-10-29. PMID:21678129. |
flow cytometry and western blot analysis indicated that borrelidin was able to increase the level of apoptosis and cause g(1) arrest in all cell lines. |
2012-10-29 |
2023-08-12 |
Not clear |
| Feng Han, Ying Luo, Yanyan Li, Zhongyang Liu, Dunquan Xu, Faguang Jin, Zhichao L. Seawater induces apoptosis in alveolar epithelial cells via the Fas/FasL-mediated pathway. Respiratory physiology & neurobiology. vol 182. issue 2-3. 2012-10-29. PMID:22609371. |
our previous study showed that seawater can cause lung tissue cell apoptosis; in the present study, the immunohistochemistry and western blot analysis results demonstrated that fas, fasl, and cleaved caspase-8 and caspase-3 were up-regulated in the rat lungs exposed to seawater. |
2012-10-29 |
2023-08-12 |
human |
| Lei Zhang, Li Tao, Junshan Ruan, Weidong Li, Yu Wu, Linggeng Yan, Feng Zhang, Fangtian Fan, Shizhong Zheng, Aiyun Wang, Yin L. Xanthatin induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma MKN-45 cells. Planta medica. vol 78. issue 9. 2012-10-26. PMID:22532019. |
western blot analysis elucidated that: a) xanthatin downregulated expression of chk1 and chk2 and phosphorylation of cdc2, which are known as key g2/m transition regulators; b) xanthatin increased p53 activation, decreased the bcl-2/bax ratio and the levels of downstream procaspase-9 and procaspase-3, which are key regulators in the intrinsic apoptosis pathway; c) xanthatin blocked phosphorylation of nf-κb (p65 subunit) and of iκbα, which might contribute to its proapoptotic effects on mkn-45 cells. |
2012-10-26 |
2023-08-12 |
human |
| Bu-Chin Yu, Wen-Jen Yu, Chun-Yung Huang, Ying-Hsin Chen, Yun-Ching Tsai, Chen-Chin Chang, Sue-Joan Chan. Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress. Evidence-based complementary and alternative medicine : eCAM. vol 2012. 2012-10-25. PMID:23093985. |
protein expressions in testes identified by proteomic analysis and verified by western blot demonstrated that tsl-a not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kda-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin a (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme a synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction). |
2012-10-25 |
2023-08-12 |
rat |
| Li Gong, Hua Yu, Yifeng ZhuGe, Qing Y. Neutrophil gelatinase-associated lipocalin protects renal tubular epithelial cell in ischemic/reperfusion injury rats via apoptosis-regulating proteins. Renal failure. vol 34. issue 6. 2012-10-24. PMID:22500534. |
the pathological changes of kidney tissues were investigated using hematoxylin-eosin staining; renal tubular epithelial cell apoptosis was detected using terminal dutp nick-labeling method; expression of apoptosis-regulating protein fas and bcl-2 was measured using real-time polymerase chain reaction, western blot, and immunohistochemical staining. |
2012-10-24 |
2023-08-12 |
rat |
| Zengning Li, Xianghong Zhang, Jinfeng Cui, Weijun Kan. Assessment on pollution of Ochratoxin A in grain in China and its apoptosis effect on vitro-cultured human tubular kidney cells. Journal of biochemical and molecular toxicology. vol 26. issue 4. 2012-10-24. PMID:22511400. |
the effects of ota on cell apoptosis, caspase-3, bax, and bcl-2 expression, and phosphorylation of c-jun nh(2) terminal kinase (jnk) were detected in cultured hkcs via flow cytometry (fcm), hoechst 33258 staining, and western blot. |
2012-10-24 |
2023-08-12 |
human |
| Guosheng Wu, Zhengming Qian, Jiajie Guo, Dejun Hu, Jiaolin Bao, Jing Xie, Wenshan Xu, Jinjian Lu, Xiuping Chen, Yitao Wan. Ganoderma lucidum extract induces G1 cell cycle arrest, and apoptosis in human breast cancer cells. The American journal of Chinese medicine. vol 40. issue 3. 2012-10-23. PMID:22745075. |
moreover, esac effectively mediated g1 cell cycle arrest in both concentration- and time-dependent manners and induced apoptosis as determined by hoechst staining, dna fragment assay and western blot analysis in mcf-7 cells. |
2012-10-23 |
2023-08-12 |
human |
| Yan-Qing Guan, Zhibin Li, Aini Yang, Zheng Huang, Zhe Zheng, Lin Zhang, Ling Li, Jun-Ming Li. Cell cycle arrest and apoptosis of OVCAR-3 and MCF-7 cells induced by co-immobilized TNF-α plus IFN-γ on polystyrene and the role of p53 activation. Biomaterials. vol 33. issue 26. 2012-10-22. PMID:22682938. |
the phosphorylation sites of the p53 regulation and the apoptosis key protein expressions of caspase 3, 8 and 9 are detected by western blot assay. |
2012-10-22 |
2023-08-12 |
Not clear |
| Seigo Miyoshi, Hironobu Hamada, Naohiko Hamaguchi, Aki Kato, Hitoshi Katayama, Kazunori Irifune, Ryoji Ito, Tatsuhiko Miyazaki, Takafumi Okura, Jitsuo Higak. Antitumor activity of MEK and PI3K inhibitors against malignant pleural mesothelioma cells in vitro and in vivo. International journal of oncology. vol 41. issue 2. 2012-10-19. PMID:22580933. |
immunohistochemical and western blot analysis of thoracic tumors suggested that these agents induced cell cycle arrest, apoptosis and inhibition of tumor angiogenesis. |
2012-10-19 |
2023-08-12 |
mouse |
| Hideo Tomioka, Toru Mukohara, Yu Kataoka, Roudy Chiminch Ekyalongo, Yohei Funakoshi, Yasuo Imai, Naomi Kiyota, Yutaka Fujiwara, Hironobu Minam. Inhibition of the mTOR/S6K signal is necessary to enhance fluorouracil-induced apoptosis in gastric cancer cells with HER2 amplification. International journal of oncology. vol 41. issue 2. 2012-10-19. PMID:22614071. |
fluorouracil-induced apoptosis was evaluated using western blot for poly (adp-ribose) polymerase (parp). |
2012-10-19 |
2023-08-12 |
Not clear |
| Yin Luo, Ran Song, Yao Li, Shuai Zhang, Zhi-Jun Liu, Jie Fu, Hai-Liang Zh. Design, synthesis, and biological evaluation of chalcone oxime derivatives as potential immunosuppressive agents. Bioorganic & medicinal chemistry letters. vol 22. issue 9. 2012-10-11. PMID:22494616. |
also, the deep mechanism of apoptosis was detected by western blot analysis. |
2012-10-11 |
2023-08-12 |
Not clear |
| Yang Hong, Meng Sang, Chao Shang, Yi-xue Xue, Yun-hui Li. Quantitative analysis of topoisomerase II alpha and evaluation of its effects on cell proliferation and apoptosis in glioblastoma cancer stem cells. Neuroscience letters. vol 518. issue 2. 2012-10-04. PMID:22569122. |
we observed the expression differences of topo-iiα gene transcript in cscs and non-cscs in glioblastoma cell line u87 by quantitative real-time pcr and western blot; we silenced topo-iiα expression in u87 cscs using topo-iiα-specific sirna to investigate the role of topo-iiα in cell proliferation, cell cycle, and apoptosis. |
2012-10-04 |
2023-08-12 |
Not clear |
| Do-Yeon Kim, Sung-Hak Kang, Sung-Ho Ghi. Cirsium japonicum extract induces apoptosis and anti-proliferation in the human breast cancer cell line MCF-7. Molecular medicine reports. vol 3. issue 3. 2012-10-02. PMID:21472257. |
furthermore, c. japonicum extract induced an anti-proliferative effect by causing g1 phase cell cycle arrest and also induced apoptosis by affecting mitochondrial apoptotic events, as determined by nuclear derangement, flow cytometry and western blot analysis. |
2012-10-02 |
2023-08-12 |
human |
| Nisha Singh, Anuraag Shrivastav, Rajendra K Sharm. Curcumin induces caspase and calpain-dependent apoptosis in HT29 human colon cancer cells. Molecular medicine reports. vol 2. issue 4. 2012-10-02. PMID:21475877. |
western blot analysis indicated that ccm induced apoptosis in ht29 cells by activating the expression of caspases-3 and -12, but not caspase-9. |
2012-10-02 |
2023-08-12 |
human |
| G Thippeswamy, B P Salimat. Induction of caspase-3 activated DNase mediated apoptosis by hexane fraction of Tinospora cordifolia in EAT cells. Environmental toxicology and pharmacology. vol 23. issue 2. 2012-10-02. PMID:21783760. |
in western blot analysis, apoptosis in the eat cells was associated with the constitutive expression of caspase activated dnase (cad) in both nucleus and cytoplasm after tchf treatment. |
2012-10-02 |
2023-08-12 |
mouse |