| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yan-Ying Li, Yan-Fang Wang, Jing Wang, Xiao-Yan K. [Effect of chidamide on human B lymphoma cell lines and its mechanisms]. Zhongguo shi yan xue ye xue za zhi. vol 20. issue 4. 2013-05-09. PMID:22931650. |
the cell proliferation was determined by cck-8 method; the cell apoptosis and mitochondrial membrane potential were analyzed by flow cytometry; the protein levels of histone h3/h4 acetylation in cells and the activity of caspase-3 were detected by western blot. |
2013-05-09 |
2023-08-12 |
human |
| Ying-Ying Yuan, Zhi-Yong Zeng, Jun-Min Che. [Effect of DAPT on proliferation and apoptosis of human multiple myeloma cell line RPMI8226]. Zhongguo shi yan xue ye xue za zhi. vol 20. issue 4. 2013-05-09. PMID:22931655. |
the proliferation of rpmi8226 cells was detected by cck-8 method; flow cytometry was employed to assay the cell apoptosis rate;the expressions of notch1 and hes1 proteins were detected by western blot. |
2013-05-09 |
2023-08-12 |
human |
| Libo Jiang, Xiaolei Zhang, Xuhao Zheng, Ao Ru, Xiao Ni, Yaosen Wu, Naifeng Tian, Yixing Huang, Enxing Xue, Xiangyang Wang, Huazi X. Apoptosis, senescence, and autophagy in rat nucleus pulposus cells: Implications for diabetic intervertebral disc degeneration. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. vol 31. issue 5. 2013-05-07. PMID:23238821. |
the apoptosis and senescence of np cells was investigated by terminal deoxyribonucleotidyl transferase (tdt)-mediated dutp-digoxigenin nick end labeling (tunel) assay, immunohistochemistry, and western blot for caspase3, caspase8, caspase9, and p16lnk4a (increased in cellular senescence). |
2013-05-07 |
2023-08-12 |
rat |
| Qiqiang Tang, Ruodong Han, Han Xiao, Jilong Shen, Qingli Luo, Jun L. Neuroprotective effects of tanshinone IIA and/or tetramethylpyrazine in cerebral ischemic injury in vivo and in vitro. Brain research. vol 1488. 2013-05-01. PMID:23063715. |
apoptosis factors (bax, bcl-2, caspase-3, and trmp-7) were analyzed using western blot and immunohistochemistry. |
2013-05-01 |
2023-08-12 |
rat |
| Sivakamasundari Pichu, Swapna Krishnamoorthy, Andrei Shishkov, Bi Zhang, Peter McCue, Biddanda C Ponnapp. Knockdown of Ki-67 by dicer-substrate small interfering RNA sensitizes bladder cancer cells to curcumin-induced tumor inhibition. PloS one. vol 7. issue 11. 2013-05-01. PMID:23152782. |
western blot analysis suggested that pretreatment with ki-67-7 sensitized bladder cancer cells to curcumin-mediated apoptosis and cell cycle arrest by p53- and p21-independent mechanisms. |
2013-05-01 |
2023-08-12 |
human |
| Haifang Xiao, Yutang Wang, Qisen Xiang, Chunxia Xiao, Li Yuan, Zhigang Liu, Xuebo Li. Novel physiological properties of ethanol extracts from Eremurus chinensis Fedtsch. roots: in vitro antioxidant and anticancer activities. Food & function. vol 3. issue 12. 2013-04-26. PMID:22948313. |
western blot analysis demonstrated that treatment with ecfe led to cell apoptosis hallmarked by parp cleavage. |
2013-04-26 |
2023-08-12 |
Not clear |
| XiaoGuang Liu, YunCheng Li, Fang Zeng, YanYan Huang, JiHang Zhou, YeKai Wang, ShiQuan Zhou, WangYu Zhu, Gang Chen, YongKui Zhan. Chan-Yu-Bao-Yuan-Tang, the water extract of a chinese medicine prescription, induces s-phase arrest and mitochondria-mediated apoptosis in human lung adenocarcinoma cells. Integrative cancer therapies. vol 11. issue 4. 2013-04-24. PMID:21382962. |
an xtt assay, cell cycle analysis, annexin v-fitc staining and western blot were applied to identify the viability of cells, cell cycle arrest, stages of apoptosis, and signaling proteins, respectively. |
2013-04-24 |
2023-08-12 |
human |
| Cui-Luan Yao, George N Somer. The impact of acute temperature stress on hemocytes of invasive and native mussels (Mytilus galloprovincialis and Mytilus californianus): DNA damage, membrane integrity, apoptosis and signaling pathways. The Journal of experimental biology. vol 215. issue Pt 24. 2013-04-22. PMID:22972891. |
western blot analyses of hemocyte extracts with antibodies for proteins associated with cell signaling and stress responses [including members of the phospho-specific mitogen-activated protein kinase (mapk) family c-jun nh(2)-terminal kinase (jnk) and p38-mapk, and apoptosis executor caspase-3] revealed that heat and cold stress induced a time-dependent activation of jnk, p38-mapk and caspase-3 and that these signaling and stress responses differed between species. |
2013-04-22 |
2023-08-12 |
Not clear |
| Xuchen Cao, Bowen Liu, Wenfeng Cao, Weiran Zhang, Fei Zhang, Hongmeng Zhao, Ran Meng, Lin Zhang, Ruifang Niu, Xishan Hao, Bin Zhan. Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells. Chinese journal of cancer research = Chung-kuo yen cheng yen chiu. vol 25. issue 2. 2013-04-18. PMID:23592903. |
flow cytometry, fluorescent staining and western blot analysis were employed to detect apoptosis and autophagy, and the role of autophagy was assessed using autophagy inhibitors. |
2013-04-18 |
2023-08-12 |
human |
| C Freiburghaus, H Lindmark-Månsson, M Paulsson, S Oredsso. Reduction of ultraviolet light-induced DNA damage in human colon cancer cells treated with a lactoferrin-derived peptide. Journal of dairy science. vol 95. issue 10. 2013-04-17. PMID:22901475. |
in the search for a mechanism, we have investigated the levels of several proteins involved in cell cycle regulation, dna replication, and apoptosis using western blot. |
2013-04-17 |
2023-08-12 |
human |
| Lan Yao, Xiaowei Nie, Shuai Shi, Shasha Song, Xuewei Hao, Shuzhen Li, Daling Zh. Reciprocal regulation of HIF-1α and 15-LO/15-HETE promotes anti-apoptosis process in pulmonary artery smooth muscle cells during hypoxia. Prostaglandins & other lipid mediators. vol 99. issue 3-4. 2013-04-16. PMID:22982617. |
furthermore, cell viability assay and several cell apoptosis assays, including tunel assay, western blot, nuclear morphology determination, mitochondrial potential analysis, indicated that blocking hif-1α induced apoptosis, decreased cell viability and suppressed the anti-apoptosis effects of 15-hete. |
2013-04-16 |
2023-08-12 |
Not clear |
| Wei-jun Pang, Yan Xiong, Yu Wang, Qiang Tong, Gong-she Yan. Sirt1 attenuates camptothecin-induced apoptosis through caspase-3 pathway in porcine preadipocytes. Experimental cell research. vol 319. issue 5. 2013-04-16. PMID:23313858. |
camptothecin induces porcine preadipocyte apoptosis in a dose-dependent manner, assessed with the hoechst staining and western blot analysis. |
2013-04-16 |
2023-08-12 |
Not clear |
| S Braig, A-K Bosserhof. Death inducer-obliterator 1 (Dido1) is a BMP target gene and promotes BMP-induced melanoma progression. Oncogene. vol 32. issue 7. 2013-04-12. PMID:22469980. |
in addition, fluorescence-activated cell sorting and western blot experiments revealed that transfection of melanoma cells with dido1 small interfering rnas led to an upregulation of apoptosis. |
2013-04-12 |
2023-08-12 |
Not clear |
| Tom Gatsinzi, Elena V Ivanova, Kerstin Iverfeld. TRAIL resistance in human neuroblastoma SK-N-AS cells is dependent on protein kinase C and involves inhibition of caspase-3 proteolytic processing. Journal of neuro-oncology. vol 109. issue 3. 2013-04-10. PMID:22798207. |
here, we show by western blot analysis, and live cell imaging using anchored fret sensors, that the resistance to trail-induced apoptosis in human neuroblastoma sk-n-as cells depends on an incomplete processing of procaspase-3, generating an immature and catalytically inactive 21 kda fragment. |
2013-04-10 |
2023-08-12 |
human |
| Shaojing Li, Chuanhong Wu, Li Zhu, Jian Gao, Jing Fang, Defeng Li, Meihong Fu, Rixin Liang, Lan Wang, Ming Cheng, Hongjun Yan. By improving regional cortical blood flow, attenuating mitochondrial dysfunction and sequential apoptosis galangin acts as a potential neuroprotective agent after acute ischemic stroke. Molecules (Basel, Switzerland). vol 17. issue 11. 2013-04-05. PMID:23143152. |
western blot analysis revealed that galangin also inhibited apoptosis in a dose-dependent manner concomitant with the up-regulation of bcl-2 expression, down-regulation of bax expression and the bax/bcl-2 ratio, a reduction in cytochrome c release from the mitochondria to the cytosol, the reduced expression of activated caspase-3 and the cleavage of poly(adp-ribose) polymerase (parp). |
2013-04-05 |
2023-08-12 |
rat |
| Shue Dong Chung, Ting Yu Lai, Chiang Ting Chien, Hong Jen Y. Activating Nrf-2 signaling depresses unilateral ureteral obstruction-evoked mitochondrial stress-related autophagy, apoptosis and pyroptosis in kidney. PloS one. vol 7. issue 10. 2013-04-04. PMID:23071780. |
fibrosis, ed-1 (macrophage/monocyte) infiltration, oxidative stress, autophagy, apoptosis and pyroptosis were evaluated by immunohistochemistry and western blot in uuo kidneys. |
2013-04-04 |
2023-08-12 |
rat |
| Sonia-Caroline Sorli, Sandra Colié, Virginie Albinet, Alexandre Dubrac, Christian Touriol, Nicolas Guilbaud, Carmen Bedia, Gemma Fabriàs, Josefina Casas, Bruno Ségui, Thierry Levade, Nathalie Andrieu-Abadi. The nonlysosomal β-glucosidase GBA2 promotes endoplasmic reticulum stress and impairs tumorigenicity of human melanoma cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 27. issue 2. 2013-03-28. PMID:23073830. |
sphingolipid levels were monitored by mass spectrometry; unfolded protein response (upr) and apoptosis were assessed by western blot and flow cytometry analyses, respectively. |
2013-03-28 |
2023-08-12 |
human |
| N Ally, X L Zou, B C Jiang, L Qin, L Zhai, P Xiao, H L Li. Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi. Genetics and molecular research : GMR. vol 11. issue 4. 2013-03-27. PMID:23212339. |
vegfa gene expression, apoptosis genes and vegfa receptor genes were detected by qrt-pcr, the vegfa protein level in culture media by elisa assay and protein levels in mgc by western blot analysis. |
2013-03-27 |
2023-08-12 |
mouse |
| Gonghua Hu, Haiyan Huang, Lingqing Yang, Caigao Zhong, Bo Xia, Yuan Yang, Jianjun Liu, Desheng Wu, Qingchen Liu, Zhixiong Zhuan. Down-regulation of Polη expression leads to increased DNA damage, apoptosis and enhanced S phase arrest in L-02 cells exposed to hydroquinone. Toxicology letters. vol 214. issue 2. 2013-03-18. PMID:22981619. |
in this study, cell survival rate was determined using the mtt assay, dna damage was determined by the comet assay, apoptosis and cell cycle distribution were determined using flow cytometry, the mrna expression levels of polη were determined by real-time pcr, and the protein expression levels of polη and γ-h2ax were determined by western blot, γ-h2ax foci were visualized by confocal laser scanning fluorescence microscopy after cells were exposed to hq at various concentrations for 24h in vitro. |
2013-03-18 |
2023-08-12 |
Not clear |
| Burçin Tezcanlı Kaymaz, Nur Selvi, Cumhur Gündüz, Cağdaş Aktan, Ayşegül Dalmızrak, Güray Saydam, Buket Kosov. Repression of STAT3, STAT5A, and STAT5B expressions in chronic myelogenous leukemia cell line K-562 with unmodified or chemically modified siRNAs and induction of apoptosis. Annals of hematology. vol 92. issue 2. 2013-03-07. PMID:23053176. |
quantitative rt-pcr and western blot assays indicated that stat expressions were downregulated both at mrna and protein levels, and tunel assay showed that leukemic cell apoptosis was induced due to inhibition of stats. |
2013-03-07 |
2023-08-12 |
Not clear |