| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Dan Liu, Bo Yi, Zhangping Liao, Lei Tang, Dong Yin, Shu Zeng, Jianguo Yao, Ming H. 14-3-3γ protein attenuates lipopolysaccharide-induced cardiomyocytes injury through the Bcl-2 family/mitochondria pathway. International immunopharmacology. vol 21. issue 2. 2015-04-09. PMID:24957688. |
the cardiomyocytes were treated with lps (1mg l(-1)) for 6h; the interaction between 14-3-3γ and phospho-bad(s112) was detected by co-immunoprecipitation (co-ip); the levels of bcl-2 family members in the cytosolic and mitochondrial fractions were examined by western blot; the apoptosis and mitochondrial membrane potential (ΔΨm) were detected by flow cytometry; and the mitochondrial permeability transition pore (mptp) opening was tested by mitochondrial swelling. |
2015-04-09 |
2023-08-13 |
Not clear |
| Kaja Urbańska, Beata Pająk, Arkadiusz Orzechowski, Justyna Sokołowska, Marta Grodzik, Ewa Sawosz, Maciej Szmidt, Paweł Sys. The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells. Nanoscale research letters. vol 10. 2015-04-08. PMID:25852394. |
assessment of cell proliferation and cell apoptosis was estimated by histological, immunohistochemical, and western blot analyses. |
2015-04-08 |
2023-08-13 |
human |
| Xin Zhao, Liang Pang, Jing Li, Jia-Le Song, Li-Hua Qi. Apoptosis inducing effects of Kuding tea polyphenols in human buccal squamous cell carcinoma cell line BcaCD885. Nutrients. vol 6. issue 8. 2015-04-01. PMID:25100434. |
by rt-pcr and western blot assays, kuding tea polyphenol significantly induced apoptosis in bcacd885 cancer cells (p < 0.05) by upregulating caspase-3, caspase-8, caspase-9, fas/fasl, bax, p53, p21, e2f1, p73 and downregulating bcl-2, bcl-xl, hiap-1, and hiap-2 mrna and protein expressions. |
2015-04-01 |
2023-08-13 |
human |
| Xi Xiao, Xiaojian Liao, Shaoling Qiu, Zihao Liu, Bin Du, Shihai X. Synthesis, cytotoxicity and apoptosis induction in human tumor cells by galaxamide and its analogues [corrected]. Marine drugs. vol 12. issue 8. 2015-03-31. PMID:25231922. |
furthermore, we used flow cytometry and western blot assays to investigate whether galaxamide and a5 could induce cancer cell apoptosis. |
2015-03-31 |
2023-08-13 |
human |
| Hyun Sook Lee, Han Jin Cho, Rina Yu, Ki Won Lee, Hyang Sook Chun, Jung Han Yoon Par. Mechanisms underlying apoptosis-inducing effects of Kaempferol in HT-29 human colon cancer cells. International journal of molecular sciences. vol 15. issue 2. 2015-03-30. PMID:24549175. |
to examine whether kaempferol induces apoptosis of ht-29 cells and to explore the underlying molecular mechanisms, cells were treated with various concentrations (0-60 μmol/l) of kaempferol and analyzed by hoechst staining, annexin v staining, jc-1 labeling of the mitochondria, immunoprecipitation, in vitro kinase assays, western blot analyses, and caspase-8 assays. |
2015-03-30 |
2023-08-12 |
human |
| Xiaoling Zhu, Shasha Jiang, Nan Hu, Fuling Luo, Hailong Dong, Yu-Ming Kang, Kyla R Jones, Yunzeng Zou, Lize Xiong, Jun Re. Tumour necrosis factor-α inhibition with lenalidomide alleviates tissue oxidative injury and apoptosis in ob/ob obese mice. Clinical and experimental pharmacology & physiology. vol 41. issue 7. 2015-03-30. PMID:24739012. |
apoptosis was evaluated using caspase 3 activity, and levels of bax, bcl-2, bip, caspase 8, caspase 9 and tnf-α were assessed using western blot analysis. |
2015-03-30 |
2023-08-13 |
mouse |
| Rasoul Ghasemi, Asadollah Zarifkar, Karim Rastegar, Nader maghsoudi, Maryam Moosav. Insulin protects against Aβ-induced spatial memory impairment, hippocampal apoptosis and MAPKs signaling disruption. Neuropharmacology. vol 85. 2015-03-30. PMID:24881967. |
after finishing of behavioral studies the hippocampi was isolated and the amount of hippocampal cleaved caspase 3 (the landmark of apoptosis) and the phosphorylated (activated) forms of p38, jnk and erk was analyzed by western blot. |
2015-03-30 |
2023-08-13 |
rat |
| Le Wang, Fu-Xin Wei, Jing-Sheng Cen, Su-Ning Ping, Zi-Qing Li, Ning-Ning Chen, Shang-Bin Cui, Yong Wan, Shao-Yu Li. Early administration of tumor necrosis factor-alpha antagonist promotes survival of transplanted neural stem cells and axon myelination after spinal cord injury in rats. Brain research. vol 1575. 2015-03-30. PMID:24887643. |
first we observed that etanercept suppressed the tnf-α expression and apoptosis of transplanted nscs by western blot, tunel and immunofluorescence staining. |
2015-03-30 |
2023-08-13 |
rat |
| Xiaochen Yu, Yang Wang, Wenhui Zhao, Haizhou Zhou, Wei Yang, Xiuru Gua. Toll-like receptor 7 promotes the apoptosis of THP-1-derived macrophages through the CHOP-dependent pathway. International journal of molecular medicine. vol 34. issue 3. 2015-03-30. PMID:24994112. |
to determine whether endoplasmic reticulum (er) stress plays a role in cell apoptosis, the mrna and protein expression of known markers of er stress [glucose-regulated protein (grp)78 and c/ebp homologous protein (chop)] was detected by reverse transcription pcr (rt-pcr), quantitative reverse transcription pcr (qrt-pcr) and western blot analysis. |
2015-03-30 |
2023-08-13 |
Not clear |
| Jian Wu, Zhigang Yu, Dechun S. BMP4 protects rat pulmonary arterial smooth muscle cells from apoptosis by PI3K/AKT/Smad1/5/8 signaling. International journal of molecular sciences. vol 15. issue 8. 2015-03-30. PMID:25110865. |
to test this hypothesis, we performed confocal, cell viability measurement, mitochondrial potential, real-time polymerase chain reaction (pcr), and western blot analysis to determine the role of bmp4 on cell survival and apoptosis. |
2015-03-30 |
2023-08-13 |
rat |
| Ying-Ying Lei, Yuan Luo, Lian-Ning Duan, Cheng-Rong Lu, Zhe Wang, Li-Ya Sun, Pei-De Xian. [Influence of calcineurin on apoptosis of pre-B lymphocytes and the leukemia cells derived from pre-B lymphocytes]. Zhongguo shi yan xue ye xue za zhi. vol 22. issue 5. 2015-03-30. PMID:25338569. |
the western blot was used to detect the effects of 2 drugs on apoptosis of s9 and s4c2 cell lines. |
2015-03-30 |
2023-08-13 |
mouse |
| Jia-Xin Fan, Ying-Jian Zeng, Jian-Wei Wu, Zhang-Qiu Li, Yuan-Ming Li, Rong Zheng, Guang-Yang Weng, Kun-Yuan Gu. [Synergistic killing effect of arsenic trioxide combined with curcumin on KG1a cells]. Zhongguo shi yan xue ye xue za zhi. vol 22. issue 5. 2015-03-30. PMID:25338570. |
the cell survival rate was mesured by mtt; colony formation capacity was examined by methylcellulose colony formation test; flow cytometry was used to analyse the cell surface molecules, cell apoptosis rate and cell cycle; the cell morphology was observed with wright-giemsa staining and the protein expression of bcl-2, bax, parp was detected by western blot. |
2015-03-30 |
2023-08-13 |
Not clear |
| Yao Yao, Yue-Ping Bian, Dan-Dan Xia, Bin Pan, Ming-Shan Niu, Kai Zhao, Ling-Yu Zeng, Kai-Lin X. [Effect of AZD8330 on proliferation and apoptosis of multiple myeloma cells]. Zhongguo shi yan xue ye xue za zhi. vol 22. issue 5. 2015-03-30. PMID:25338579. |
the wester blot was used to detect the expression levels of cyclin d and cyclin e, and multiple myeloma cells were treated with 10, 100, 1000 and 2000 nmol/l of azd8330, the annexinv/7-aad double staining was used to analyse cell apoptosis and the western blot was used to detect the expression level of caspase-3. |
2015-03-30 |
2023-08-13 |
Not clear |
| Ying Wang, Yu-Xiang Han, Zhi-Yun Niu, Xing-Zhe Wang, Huan Hua, Yin-Tao Shang, Fu-Xu Wang, Xue-Jun Zhang, Jian-Min Lu. [BMMSC from blastic phase CML down-regulate leukemia cell apoptosis]. Zhongguo shi yan xue ye xue za zhi. vol 22. issue 5. 2015-03-30. PMID:25338597. |
k562 cells and primary cml-bp cells were co-cultured with bmmsc of different groups; the cell proliferation was detected by mtt method, the cell apoptosis rate and mitochondrial membrane potential were measured by flow cytometry, the expression levels of caspase-8, caspase-9, and activated caspase-3 in cells were measured by western blot. |
2015-03-30 |
2023-08-13 |
Not clear |
| Zhikun Sun, Xingrong Ma, Hongqi Yang, Jiahua Zhao, Jiewen Zhan. Brain-derived neurotrophic factor prevents beta- amyloid-induced apoptosis of pheochromocytoma cells by regulating Bax/Bcl-2 expression. Neural regeneration research. vol 7. issue 5. 2015-03-16. PMID:25774173. |
methylthiazolyldiphenyl-tetrazolium bromide assay and western blot analysis showed that brain-derived neurotrophic factor provided neuroprotection against cellular apoptosis by suppressing the decline in β-amyloid(25-35)-induced cell activity and the increasing ratio of bax/bcl-2. |
2015-03-16 |
2023-08-13 |
Not clear |
| Yue Xing, Di Yang, Jing Lu, De-Li Don. Insulin prevents bone morphogenetic protein-4 induced cardiomyocyte apoptosis through activating Akt. Biochemical and biophysical research communications. vol 456. issue 2. 2015-03-13. PMID:25490386. |
cell viability and apoptosis were measured by using mtt, live and dead staining, caspase-3 activity assays, and the protein expressions were measured by using western blot technique. |
2015-03-13 |
2023-08-13 |
Not clear |
| Guo-Fu Huang, Jing Zou, Jing Shi, Dong-You Zhang, Hong-Fen Peng, Qi Zhang, Yu Gao, Bo-Yi Wang, Tang-Fa Zhan. Electroacupuncture stimulates remodeling of extracellular matrix by inhibiting apoptosis in a rabbit model of disc degeneration. Evidence-based complementary and alternative medicine : eCAM. vol 2015. 2015-03-12. PMID:25763091. |
apoptosis involvement was determined with tunel staining and western blot for the protein expression of bax and bcl-2. |
2015-03-12 |
2023-08-13 |
rabbit |
| Gabriella Teti, Viviana Salvatore, Maria Carla Mazzotti, Beatrice Orlandini, Stefano Focaroli, Sandra Durante, Ferdinando Paternostr. Ultrastructural changes in human gingival fibroblasts after exposure to 2-hydroxy-ethyl methacrylate. Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia. vol 119. issue 2. 2015-03-10. PMID:25665282. |
primary cultures of gingival fibroblasts were exposed to 3mm hema for 24 h, 72 h, 96 h. morphological investigations were performed by scanning and transmission electron microscopy, while western blot for caspase-3 was carried out to ver- ify apoptosis. |
2015-03-10 |
2023-08-13 |
human |
| Yong-Xin Li, Kyong-Hwa Kang, Hak Jun Kim, Se-Kwon Ki. In vitro induction of apoptosis by isosclerone from marine-derived fungus Aspergillus fumigatus. Bioorganic & medicinal chemistry letters. vol 24. issue 16. 2015-03-06. PMID:25011914. |
western blot and rt-pcr analysis have indicated that isosclerone induce cancer cells apoptosis through down-regulated bcl-2 family and up-regulated caspases, and activating the nf-κ-b signaling pathway. |
2015-03-06 |
2023-08-13 |
human |
| Euri Ahn, Ha Kim, Kyusun Torque Han, Jeong-Im Si. A loss of antitumor therapeutic activity of CEA DNA vaccines is associated with the lack of tumor cells' antigen presentation to Ag-specific CTLs in a colon cancer model. Cancer letters. vol 356. issue 2 Pt B. 2015-03-03. PMID:25449428. |
finally, our in vitro assays (western blot assay, ifn-γ, ctl and apoptosis assays, facs analysis) and animal studies demonstrated that a lack of antitumor therapeutic activity of cea dna vaccines might result from acquisition of tumor cell resistance to ag-specific ctl-mediated killing through the loss of tumor cells' antigen presentation to ag-specific ctls. |
2015-03-03 |
2023-08-13 |
human |