| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Lin Yang, Jian-Min Luo, Xiao-Jun Liu, Shu-Peng Wen, Xing-Yan Du, Li Yao, Jing-Ci Yan. [The mechanism for SHIP gene to induce the apoptosis of human leukemia cell line K562.]. Sheng li xue bao : [Acta physiologica Sinica]. vol 61. issue 2. 2015-05-16. PMID:19377826. |
the results are as follows: (1) human leukemia cell line k562 was ship-negative; (2) transfection with ship gene led to the re-expression of ship mrna and protein in k562, as shown by fq-pcr and western blot; (3) the expression of ship protein inhibited cell growth and significantly increased apoptosis in k562 cells; (4) compared to that in control group, the expression level of p-akt-308 and p-akt-473 in ship-expressing cell group decreased significantly (p<0.01); ship activated caspase-9, caspase-3, up-regulated protein levels of bad, p27, down-regulated expression of bcl-xl, while it had no effect on the expression of bcl-2 and bax. |
2015-05-16 |
2023-08-12 |
human |
| Hong-Nan Huo, Kun-Peng Xie, Li-Meng Wang, Ying Jiang, Wei Zou, Ming-Jie Xi. [Relationship between the inhibitory effect of fraxetin on breast cancer and estrogen signaling pathway]. Sheng li xue bao : [Acta physiologica Sinica]. vol 65. issue 3. 2015-05-16. PMID:23788190. |
the article used mtt assay, flow cytometer analysis and western blot to detect the inhibition of fraxetin on mcf-7 cell cycle distribution and apoptosis, erα, cyclin d1 and bcl-2 expression levels, mapk and pi3k signaling pathway to investigate the mechanism of anti-breast cancer of fraxetin. |
2015-05-16 |
2023-08-12 |
Not clear |
| Jianguo Li, Li Lin, Liqing Du, Chang Xu, Yan Wang, Jia Cao, Qin Wang, Feiyue Fan, Xiaoping Wang, Yafei Wang, Qiang Li. Radioprotective effect of a pan-caspase inhibitor in a novel model of radiation injury to the nucleus of the abducens nerve. Molecular medicine reports. vol 10. issue 3. 2015-05-13. PMID:24939579. |
the present study performed immunohistochemistry, terminal deoxynucleotidyl transferase dutp nick end labeling (tunel) and western blot analysis and identified no significant changes in the expression of the x-linked inhibitor of apoptosis protein (xiap) following radiation (p>0.05). |
2015-05-13 |
2023-08-13 |
rat |
| Xiuqing Xiao, Hao He, Xuezhi Ding, Qi Yang, Xuemei Liu, Shuang Liu, Jie Rang, Ting Wang, Mingxing Zuo, Liqiu Xi. Purification and cloning of lectin that induce cell apoptosis from Allium chinense. Phytomedicine : international journal of phytotherapy and phytopharmacology. vol 22. issue 2. 2015-05-13. PMID:25765828. |
western blot showed that acl upregulated caspase-3 and bax expression during apoptosis and cloned the structural gene of acl with an open reading frame of 456 bp encoding 151 amino acid residues. |
2015-05-13 |
2023-08-13 |
human |
| Zheng-Xiang Han, Xiao-Xia Wang, Shang-Nuan Zhang, Jin-Xia Wu, He-ya Qian, Yi-yang Wen, Hui Tian, Dong-Sheng Pei, Jun-Nian Zhen. Downregulation of PAK5 inhibits glioma cell migration and invasion potentially through the PAK5-Egr1-MMP2 signaling pathway. Brain tumor pathology. vol 31. issue 4. 2015-05-12. PMID:24062079. |
additionally, flow cytometry and western blot analysis indicated that pak5 could inhibit cell apoptosis. |
2015-05-12 |
2023-08-12 |
human |
| Ke-Wang Luo, Chun-Hay Ko, Grace Gar-Lee Yue, Julia Kin-Ming Lee, Kai-Kai Li, Michelle Lee, Gang Li, Kwok-Pui Fung, Ping-Chung Leung, Clara Bik-San La. Green tea (Camellia sinensis) extract inhibits both the metastasis and osteolytic components of mammary cancer 4T1 lesions in mice. The Journal of nutritional biochemistry. vol 25. issue 4. 2015-05-12. PMID:24561153. |
western blot analysis showed that cs increased the expression of bax-to-bcl-2 ratio and activated caspase-8 and caspase-3 to induce apoptosis. |
2015-05-12 |
2023-08-12 |
mouse |
| Weiguo Jian, Hua-Guo Xu, Jianfeng Chen, Zhi-Xiang Xu, Jonathan M Levitt, Jennifer A Stanley, Eddy S Yang, Seth P Lerner, Guru Sonpavd. Activity of CEP-9722, a poly (ADP-ribose) polymerase inhibitor, in urothelial carcinoma correlates inversely with homologous recombination repair response to DNA damage. Anti-cancer drugs. vol 25. issue 8. 2015-05-12. PMID:24714082. |
flow cytometry, comet assay, and western blot were performed to assess apoptosis, dna damage, and dna-repair proteins, respectively. |
2015-05-12 |
2023-08-13 |
human |
| Chao-Ying Cheng, Yan-Yan Feng, Yi Zang, Jia Li, Xiao-Peng He, Guo-Rong Che. Identification of a new bis-amino acid glycoside selectively toxic to multiple myeloma cells. Carbohydrate research. vol 394. 2015-05-12. PMID:24972313. |
a western blot analysis suggested that this compound could potentiate the cleavage of poly adp-ribose polymerase in mm cells, leading to apoptosis. |
2015-05-12 |
2023-08-13 |
Not clear |
| Qiang Yu, Qiaoyu Li, Peisong Lu, Qianxue Che. Polyphyllin D induces apoptosis in U87 human glioma cells through the c-Jun NH2-terminal kinase pathway. Journal of medicinal food. vol 17. issue 9. 2015-05-12. PMID:25045920. |
the apoptosis of u87 glioma cells was detected by flow cytometry, and western blot analysis was used to examine human b-cell leukemia/lymphoma 2 (bcl-2), human bcl-2 associated x protein (bax), caspase-3, total-c-jun nh2-terminal kinase (t-jnk), and phosphorylation-jnk (p-jnk) protein expression in u87 human glioma cells. |
2015-05-12 |
2023-08-13 |
human |
| S Ray, K Chatterjee, D De, D Ghos. Bioefficacy of hydromethanolic extract of tuber of Chlorophytum borivilianum (Safed Musli) for the management of male infertility in cyproterone acetate-treated albino rats. Andrologia. vol 46. issue 6. 2015-05-11. PMID:23957231. |
apoptosis profile was evaluated by western blot study, tunel assay and dna fragmentation study of testicular tissues. |
2015-05-11 |
2023-08-12 |
rat |
| Ping Zhang, He-Sheng Luo, Ming Li, Shi-Yun Ta. Artesunate inhibits the growth and induces apoptosis of human gastric cancer cells by downregulating COX-2. OncoTargets and therapy. vol 8. 2015-05-06. PMID:25945055. |
the effects of artesunate on the growth and apoptosis of gastric cancer cells were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometric analysis of annexin v-fluorescein isothiocyanate/propidium iodide staining, rhodamine 123 staining, and western blot analysis. |
2015-05-06 |
2023-08-13 |
human |
| Wen-Jing Zhou, Qi-Feng Gui, Yue Wu, Yun-Mei Yan. Tanshinone IIA protects against methylglyoxal-induced injury in human brain microvascular endothelial cells. International journal of clinical and experimental medicine. vol 8. issue 2. 2015-05-01. PMID:25932127. |
annexinv/pi staining and western blot were performed to determine cell apoptosis and protein expression. |
2015-05-01 |
2023-08-13 |
human |
| Ke Chen, Yue-Hua Yang, Sheng-Dan Jiang, Lei-Sheng Jian. Decreased activity of osteocyte autophagy with aging may contribute to the bone loss in senile population. Histochemistry and cell biology. vol 142. issue 3. 2015-04-22. PMID:24553790. |
immunofluorescent histochemistry, rt-pcr, and western blot assessment demonstrated that the expression of lc3-ii, lc3-ii/i, beclin-1, and ulk-1 in the osteocytes decreased with age, while sqstm1/p62 and apoptosis in the osteocytes increased. |
2015-04-22 |
2023-08-12 |
rat |
| Xing-Qi Li, Zhi-Gang Ouyang, Sheng-Hua Zhang, Hong Liu, Yue Shang, Yi Li, Yong-Su Zhe. Synergistic inhibition of angiogenesis and glioma cell-induced angiogenesis by the combination of temozolomide and enediyne antibiotic lidamycin. Cancer biology & therapy. vol 15. issue 4. 2015-04-20. PMID:24424202. |
as determined by western blot and elisa, the interaction of tumor cells and the rbmec was markedly interrupted by ldm plus tmz with synergistic regulations of vegf induced angiogenesis signal pathway, tumor cell invasion/migration, and apoptosis signal pathway. |
2015-04-20 |
2023-08-12 |
human |
| Sha Yi, Lu Wen, Jing He, Youping Wang, Fei Zhao, Jie Zhao, Zichu Zhao, Guohui Cui, Yan Che. Deguelin, a selective silencer of the NPM1 mutant, potentiates apoptosis and induces differentiation in AML cells carrying the NPM1 mutation. Annals of hematology. vol 94. issue 2. 2015-04-20. PMID:25242579. |
drug efficacy was evaluated by apoptosis and differentiation assays, and associated molecular events were assessed by western blot. |
2015-04-20 |
2023-08-13 |
human |
| Peipei An, Yu Ding, Aibing Wang, Wentao Tan, Fei Gao, Anran Fan, Bo Tang, Xueming Zhang, Ziyi L. Effects of WT1 gene downregulation on apoptosis in porcine fetal fibroblasts. In vitro cellular & developmental biology. Animal. vol 50. issue 6. 2015-04-16. PMID:24652047. |
to further explore whether wt1 was expressed in porcine fetal fibroblasts (pffs) and its contribution to cell apoptosis, rt-pcr, immunocytochemical staining, and western blot were used to detect the expression of wt1, the recombinant plasmids of plv3-wt1 short hairpin ribonucleic acid (shrna) were used to downregulate the wt1 gene in porcine fetal fibroblasts, and the role of wt1 in cell proliferation was examined by apoptosis analysis also. |
2015-04-16 |
2023-08-12 |
Not clear |
| Xiaodong Wang, Arong Jin, Min An, Yumei Ding, Ya Tuo, Yi Qi. Etomidate deteriorates the toxicity of advanced glycation end products to human endothelial Eahy926 cells. The Journal of toxicological sciences. vol 39. issue 6. 2015-04-16. PMID:25421967. |
in addition, western blot assay of apoptosis-associated molecules indicated that both agents synergistically upregulated the cytochrome c release, activated the apoptosis executor, caspase 3, and promoted the poly-adp-ribose polymerase (parp) lysis. |
2015-04-16 |
2023-08-13 |
human |
| Xiaoyun Zhang, Qizhu Tang, Li X. Herpes simplex virus 2 infects human endothelial ECV304 cells and induces cell apoptosis synergistically with ox-LDL. The Journal of toxicological sciences. vol 39. issue 6. 2015-04-16. PMID:25421969. |
moreover, the western blot assay confirmed the apoptosis induction; there was a significantly high level of released cytochrome c, activated caspase 3 and lyzed poly (adp-ribose) polymerase (parp) by the activated caspase 3 in the hsv-2-infected ecv304 cells, particularly the cells subject to ox-ldl. |
2015-04-16 |
2023-08-13 |
human |
| Dinesh Thummuri, Manish Kumar Jeengar, Shweta Shrivastava, Aparna Areti, Veera Ganesh Yerra, Samyuktha Yamjala, Prashanth Komirishetty, V G M Naidu, Ashutosh Kumar, Ramakrishna Sistl. Boswellia ovalifoliolata abrogates ROS mediated NF-κB activation, causes apoptosis and chemosensitization in Triple Negative Breast Cancer cells. Environmental toxicology and pharmacology. vol 38. issue 1. 2015-04-13. PMID:24908637. |
apoptotic studies showed that bl ethoh was able to induce apoptosis and western blot studies demonstrated that bl ethoh significantly decreased the phospho-nf-κb (ser536), pcna, anti-apoptotic protein bcl-2 expression and increased the expression of pro-apoptotic protein bax, in mda-mb-231 and mda-mb-453 cell lines when compared with untreated cells. |
2015-04-13 |
2023-08-13 |
Not clear |
| Yong-Zhan Zhen, Ya-Jun Lin, Kai-Ji Li, Xiao-Shan Yang, Yu-Fang Zhao, Jie Wei, Jing-Bo Wei, Gang H. Gambogic Acid lysinate induces apoptosis in breast cancer mcf-7 cells by increasing reactive oxygen species. Evidence-based complementary and alternative medicine : eCAM. vol 2015. 2015-04-13. PMID:25866542. |
inhibition of cell proliferation was determined by mtt assay; intracellular ros level was detected by staining cells with dcfh-da; cell apoptosis was determined by flow cytometer and the mechanism of gal was investigated by western blot. |
2015-04-13 |
2023-08-13 |
human |