| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| T Y Long, R Jing, F Kuang, L Huang, Z X Qian, T L Yan. CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 50. issue 4. 2017-09-06. PMID:28355355. |
subsequently, alamarblue assay, flow cytometry and western blot analysis were used, respectively, to assess cell viability, reactive oxygen species (ros) level and apoptosis, and expression levels of iκbα, p65 and bcl-3. |
2017-09-06 |
2023-08-13 |
Not clear |
| Zongyi Xie, Lei Huang, Budbazar Enkhjargal, Cesar Reis, Weifeng Wan, Jiping Tang, Yuan Cheng, John H Zhan. Intranasal administration of recombinant Netrin-1 attenuates neuronal apoptosis by activating DCC/APPL-1/AKT signaling pathway after subarachnoid hemorrhage in rats. Neuropharmacology. vol 119. 2017-08-31. PMID:28347836. |
sah grade, neurological score, neuronal apoptosis assessed by cleaved caspase-3 (cc-3) expression and fluoro-jade c (fjc) staining, double immunofluorescence staining, and western blot were examined. |
2017-08-31 |
2023-08-13 |
human |
| Yan Zhang, Rong Zhang, Xixi Ding, Bangan Peng, Ning Wang, Fang Ma, Youmei Peng, Qingduan Wang, Junbiao Chan. FNC efficiently inhibits mantle cell lymphoma growth. PloS one. vol 12. issue 3. 2017-08-22. PMID:28333959. |
in in vitro experiments, cell viability was detected by the cck8 assay, and cell cycle progression and apoptosis were assessed by flow cytometry, and the expression of relative apoptosis proteins were detected by western blot. |
2017-08-22 |
2023-08-13 |
mouse |
| Xiao-Qiang Wang, Juliette A Aka, Tang Li, Dan Xu, Charles J Doillon, Sheng-Xiang Li. Inhibition of 17beta-hydroxysteroid dehydrogenase type 7 modulates breast cancer protein profile and enhances apoptosis by down-regulating GRP78. The Journal of steroid biochemistry and molecular biology. vol 172. 2017-08-22. PMID:28645527. |
cell apoptosis was analyzed by flow cytometry, followed by reverse transcription quantitative real-time pcr (rt-qpcr) and western blot to investigate the expression of apoptosis-related genes. |
2017-08-22 |
2023-08-13 |
Not clear |
| Tingting Ren, Juanjuan Zhu, Lili Zhu, Mingliang Chen. The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-α. Nutrients. vol 9. issue 3. 2017-08-17. PMID:28264426. |
the levels of peroxisome proliferators-activated receptor (ppar)-α, sterol regulatory element binding protein-1c (srebp-1c), patatin-like phospholipase domain-containing protein 3 (pnpla-3), inflammatory cytokines and apoptosis biomarkers in liver tissues were measured by qrt-pcr and western blot. |
2017-08-17 |
2023-08-13 |
rat |
| Shiva Mansouri, Ingrid Agartz, Sven-Ove Ögren, Cesare Patrone, Mathias Lundber. PACAP Protects Adult Neural Stem Cells from the Neurotoxic Effect of Ketamine Associated with Decreased Apoptosis, ER Stress and mTOR Pathway Activation. PloS one. vol 12. issue 1. 2017-08-14. PMID:28125634. |
after 24 hours, cell viability, potential involvement of apoptosis, endoplasmic reticulum (er) stress, mtor and ampa pathway activation were assessed by quantitative rt-pcr and western blot analysis. |
2017-08-14 |
2023-08-13 |
mouse |
| Ran Jing, Zhengming Zhou, Feng Kuang, Lei Huang, Chuanchang L. microRNA-99a Reduces Lipopolysaccharide-Induced Oxidative Injury by Activating Notch Pathway in H9c2 Cells. International heart journal. vol 58. issue 3. 2017-08-09. PMID:28484120. |
western blot was performed to determine the expression of notch pathway factors.lps could significantly suppress viability and increase cell death, apoptosis, and ros level (p < 0.05). |
2017-08-09 |
2023-08-13 |
rat |
| Lili Jia, Fei Wang, Xiangqian Gu, Yiqi Weng, Mingwei Sheng, Gang Wang, Shipeng Li, Hongyin Du, Wenli Y. Propofol postconditioning attenuates hippocampus ischemia-reperfusion injury via modulating JAK2/STAT3 pathway in rats after autogenous orthotropic liver transplantation. Brain research. vol 1657. 2017-08-07. PMID:27998796. |
we evaluated histological damage, inflammation, oxidative stress and apoptosis in hippocampus using he staining, light microscope, real-time pcr and western blot. |
2017-08-07 |
2023-08-13 |
rat |
| Lei Shi, Ruigao Song, Xiaolei Yao, Youshe Re. Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig cells in vitro. Theriogenology. vol 93. 2017-08-01. PMID:28257863. |
the mrna expression and protein abundance of cell cycle, apoptosis and testosterone synthesis-related genes were detected using real-time pcr and western blot analysis. |
2017-08-01 |
2023-08-13 |
Not clear |
| Mohsen Marvibaigi, Neda Amini, Eko Supriyanto, Fadzilah Adibah Abdul Majid, Saravana Kumar Jaganathan, Shajarahtunnur Jamil, Javad Hamzehalipour Almaki, Rozita Nasir. Antioxidant Activity and ROS-Dependent Apoptotic Effect of Scurrula ferruginea (Jack) Danser Methanol Extract in Human Breast Cancer Cell MDA-MB-231. PloS one. vol 11. issue 7. 2017-07-31. PMID:27410459. |
the present study demonstrated that the s. ferruginea methanol extract mediated mda-mb-231 cell growth inhibition via induction of apoptosis which was confirmed by western blot analysis. |
2017-07-31 |
2023-08-13 |
human |
| Xudan Shi, Liang Xu, Desislava Met Doycheva, Jiping Tang, Min Yan, John H Zhan. Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. vol 37. issue 4. 2017-07-26. PMID:27381825. |
western blot showed that sestrin2 expression levels were increased after treatment with rh-sestrin2, and sestrin2 exerts neuroprotective effects via activation of the adenosine monophosphate-activated protein kinase pathway which in turn inhibits mammalian target of rapamycin signaling resulting in the attenuation of apoptosis. |
2017-07-26 |
2023-08-13 |
human |
| Agnieszka Galanty, Paulina Koczurkiewicz, Dawid Wnuk, Milena Paw, Elżbieta Karnas, Irma Podolak, Michał Węgrzyn, Magdalena Borusiewicz, Zbigniew Madeja, Jarosław Czyż, Marta Michali. Usnic acid and atranorin exert selective cytostatic and anti-invasive effects on human prostate and melanoma cancer cells. Toxicology in vitro : an international journal published in association with BIBRA. vol 40. 2017-07-26. PMID:28095330. |
the aim of the study was focused on the impact of the two lichen metabolites, on the viability (by trypan blue test and fluoresceine diacetate and ethidium bromide assay), proliferation (cell counting in a bürker's chamber), apoptosis (flow cytometry analysis and western blot) and motile activity (cell movement recording and image analysis) and actin cytoskeleton organization (immunofluorescent staining) of melanoma htb-140, prostate cancers du-145 and pc-3, normal human skin fibroblasts and prostate epithelial pnt2 cells, with special emphasis to their selectivity and versatility. |
2017-07-26 |
2023-08-13 |
human |
| Lan Wu, Kai Bao, Rui Song, Defa Wang, Lei Zhang, Weiyun Wang, Weige Zhang, Wen Bi. Development of Novel Erythromycin Derivatives with Inhibitory Activity against Proliferation of Tumor Cells. PloS one. vol 11. issue 7. 2017-07-24. PMID:27447724. |
further experiments including morphologic observation, dna agarose gel electrophoresis, mitochondrial potential alternation and western blot analysis revealed that the antiproliferative mechanism may involve the induction of apoptosis in activating the mitochondrial pathway, and regulation of apoptotic proteins. |
2017-07-24 |
2023-08-13 |
human |
| Barbara Bassani, Desirèe Bartolini, Arianna Pagani, Elisa Principi, Massimo Zollo, Douglas M Noonan, Adriana Albini, Antonino Brun. Fenretinide (4-HPR) Targets Caspase-9, ERK 1/2 and the Wnt3a/β-Catenin Pathway in Medulloblastoma Cells and Medulloblastoma Cell Spheroids. PloS one. vol 11. issue 7. 2017-07-19. PMID:27367907. |
western blot analysis were used to investigate the crucial molecules involved in selected signaling pathways associated with apoptosis (caspase-9 and parp-1), cell survival (erk 1/2) and tumor progression (wnt3a and β-catenin). |
2017-07-19 |
2023-08-13 |
mouse |
| Soukaina Alaoui, Maeva Dufies, Mohsine Driowya, Luc Demange, Khalid Bougrin, Guillaume Robert, Patrick Auberger, Gilles Pagès, Rachid Benhid. Synthesis and anti-cancer activities of new sulfonamides 4-substituted-triazolyl nucleosides. Bioorganic & medicinal chemistry letters. vol 27. issue 9. 2017-07-18. PMID:28325600. |
western blot analyses on rcc4 cells showed that 5i displayed an interesting mode of action by inducing both apoptosis and autophagy cell death, making therefore this class of molecules highly promising for further hit-to-lead optimization. |
2017-07-18 |
2023-08-13 |
Not clear |
| B S Betts-Obregon, A A Mondragon, A S Mendiola, R G LeBaron, R Asmis, T Zou, F Gonzalez-Fernandez, A T Tsi. TGFβ induces BIGH3 expression and human retinal pericyte apoptosis: a novel pathway of diabetic retinopathy. Eye (London, England). vol 30. issue 12. 2017-07-17. PMID:27564721. |
in the present study, we propose a novel pathway in which pericyte apoptosis is mediated by macrophages, tgfβ and pro-apoptotic bigh3 (tgfβ-induced gene human clone 3) protein.patients and methodsto elucidate this pathway, we assayed human retinal pericyte (hrp) apoptosis by tunel assay, bigh3 mrna expression by qpcr, and bigh3 protein expression by western blot analysis. |
2017-07-17 |
2023-08-13 |
human |
| Linlin Wang, Tingting Wang, Haobo Li, Qing Liu, Zhongjun Zhang, Wanli Xie, Yinglu Feng, Tumenjavkhlan Socorburam, Gui Wu, Zhengyuan Xia, Qingping W. Receptor Interacting Protein 3-Mediated Necroptosis Promotes Lipopolysaccharide-Induced Inflammation and Acute Respiratory Distress Syndrome in Mice. PloS one. vol 11. issue 5. 2017-07-13. PMID:27195494. |
lung tissues were harvested for histological and tunel staining and western blot for rip3, p-rip3, x-linked inhibitor of apoptosis protein (xiap), mixed lineage kinase domain-like protein (mlkl), total and cleaved caspases-3/8. |
2017-07-13 |
2023-08-13 |
mouse |
| Yaping Xie, Dan Yu, Jiahua Wu, Lin L. Protective effects of physiological testosterone on advanced glycation end product‑induced injury in human endothelial cells. Molecular medicine reports. vol 15. issue 3. 2017-07-13. PMID:28112379. |
early‑stage apoptosis was detected using flow cytometry with annexin v‑fluorescein isothiocyanate/propidium iodide double staining, and the expression levels of apoptosis‑associated proteins, b cell lymphoma‑2 (bcl‑2), bcl‑2‑associated x protein (bax) and caspase‑3, were determined using western blot analysis. |
2017-07-13 |
2023-08-13 |
human |
| Xin Yan, Qianyin Li, Dongsheng Ni, Yajun Xie, Qingling He, Qianya Wan, Yamin Liu, Zhongshi Lyu, Zhaomin Mao, Qin Zho. Apobec-1 complementation factor regulates cell migration and apoptosis through Dickkopf1 by acting on its 3' untranslated region in MCF7 cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 39. issue 6. 2017-07-11. PMID:28639893. |
wound healing assay and flow cytometry were applied to detect the migration and apoptosis; western blot, real-time polymerase chain reaction, and dual-luciferase assays were applied to investigate the potential regulation mechanism of a1cf-mediated cell migration and apoptosis. |
2017-07-11 |
2023-08-13 |
human |
| Sung Won Lee, Jee Hyun Rho, Sang Yeob Lee, Seung Hee Yoo, Hye Young Kim, Won Tae Chung, Young Hyun Yo. Alpha B-Crystallin Protects Rat Articular Chondrocytes against Casein Kinase II Inhibition-Induced Apoptosis. PloS one. vol 11. issue 11. 2017-06-26. PMID:27851782. |
to examine whether the silencing of αb-crystallin sensitizes rat articular chondrocytes to ck2 inhibition-induced apoptosis, we assessed apoptosis by performing viability assays, mitochondrial membrane potential measurements, flow cytometry, nuclear morphology observations, and western blot analysis. |
2017-06-26 |
2023-08-13 |
rat |