| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wei Ji, Shijia Liu, Xia Zhao, Yunke Guo, Sha Xia, Yueyang Lu, Menyun Yin, Xiao X. Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells. Molecular medicine reports. vol 16. issue 5. 2018-06-06. PMID:28944904. |
cell proliferation was detected using the bromodeoxyuridine assay, cell cycle and apoptosis were measured by flow cytometry, nuclear apoptosis was observed by hoechst staining and associated proteins were determined via western blot analysis. |
2018-06-06 |
2023-08-13 |
mouse |
| Yuxin Chen, Ling Zhu, Xi Yang, Cheng Wei, Chuanrong Chen, Yang He, Zhaoning J. Ailanthone induces G2/M cell cycle arrest and apoptosis of SGC‑7901 human gastric cancer cells. Molecular medicine reports. vol 16. issue 5. 2018-06-04. PMID:28901518. |
cell cycle progression and apoptosis were measured by flow cytometry, and the protein and mrna expression levels of bcl‑2 and bax were analyzed by western blot analysis and reverse transcription‑quantitative polymerase chain reaction (rt‑qpcr) respectively, in sgc‑7901 cells. |
2018-06-04 |
2023-08-13 |
human |
| Li-Juan Wei, Jun-An Li, Dong-Mei Bai, Ying Son. miR-223-RhoB signaling pathway regulates the proliferation and apoptosis of colon adenocarcinoma. Chemico-biological interactions. vol 289. 2018-06-04. PMID:29660302. |
we further used western blot to determine levels of cell cycle regulators cdk1 and cyclin b1 with anti-mir-223 or apoptosis with overexpression of rhob. |
2018-06-04 |
2023-08-13 |
human |
| Silvia Tomkova, Matus Misuth, Lenka Lenkavska, Pavol Miskovsky, Veronika Huntosov. In vitro identification of mitochondrial oxidative stress production by time-resolved fluorescence imaging of glioma cells. Biochimica et biophysica acta. Molecular cell research. vol 1865. issue 4. 2018-06-01. PMID:29410069. |
their involvement in oxidative phosphorylation and apoptosis was investigated with oxygen consumption rate estimation, western blot and flow-cytometric analysis. |
2018-06-01 |
2023-08-13 |
Not clear |
| Xiao-Xia Liu, Jing Xia, Jia-Feng Tang, Ming-Hua Zhou, Di-Long Chen, Ze-Hong Li. [Ginsenoside Rh₂ induces apoptosis and autophagy of K562 cells by activating p38]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 42. issue 1. 2018-05-31. PMID:28945040. |
cck-8 assay was used to examine the growth inhibition of human leukemia cell lines k562 treated with ginsenoside rh₂; flow cytometry (fcm) was used to detect cell apoptosis; hoechst staining was used to observe the changes of cell morphological apoptosis; acridine and mdc staining were used to detect the effects of the rh₂ on autophagy; western blot and rt-pcr were used to detect the expression levels of the proteins closely associated with autophagy and apoptosis. |
2018-05-31 |
2023-08-13 |
human |
| Xiao-Xia Liu, Jing Xia, Jia-Feng Tang, Ming-Hua Zhou, Di-Long Chen, Ze-Hong Li. [Ginsenoside Rh₂ induces apoptosis and autophagy of K562 cells by activating p38]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 42. issue 1. 2018-05-31. PMID:28945040. |
in order to study the effect of autophagy in proliferation and apoptosis, we used the autophagy inhibitor (3-ma).cck-8 indicated that rh₂ at low concentration could effectively inhibit the proliferation of leukemia cellsin dose- and time-dependent manners in k562 cells; fcm indicated that rh₂ induced apoptosis; hoechest staining showed that k562 cells had typical apoptotic morphological changes by treated rh₂; acridine and mdc staining showed that rh₂ enhanced the green fluorescence and a large number of acidic autophagy vesicles were present; western blot and rt-pcr results showed that rh₂ increased the expression levels of beclin-1, lc3a, lc3b, activated caspase-3 and p-p38 in k562 cells; application of autophagy inhibitors(3-ma) could weaken the inhibition effect of rh₂ on proliferation and induction effect on apoptosis in k562 cells. |
2018-05-31 |
2023-08-13 |
human |
| Qing Su, Yu Wang, Xin Yang, Xiao-Dong Li, Yong-Fen Qi, Xiao-Jing He, Yan-Jie Wan. Inhibition of Endoplasmic Reticulum Stress Apoptosis by Estrogen Protects Human Umbilical Vein Endothelial Cells Through the PI3 Kinase-Akt Signaling Pathway. Journal of cellular biochemistry. vol 118. issue 12. 2018-05-29. PMID:28485890. |
the effect of estrogen on ers apoptosis, the mechanism responsible for that effect, and the ers signaling pathways were examined in human umbilical vein endothelial cells (huvecs) and measured using western blot, hoechst stains and caspase-3 activity assay. |
2018-05-29 |
2023-08-13 |
human |
| Weiwei Zhang, Ying Li, Peng Wan. Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 51. issue 6. 2018-05-29. PMID:29694511. |
cell viability, apoptosis, apoptosis-associated factors, and p38/mapk pathway were examined by mtt, flow cytometry, and western blot assays. |
2018-05-29 |
2023-08-13 |
human |
| Honglei Chen, Zhixu Zhang, Li Zhang, Junzhi Wang, Minghui Zhang, Bin Zh. miR-27a protects human mitral valve interstitial cell from TNF-α-induced inflammatory injury via up-regulation of NELL-1. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 51. issue 6. 2018-05-29. PMID:29694513. |
trypan blue staining, brdu incorporation assay, flow cytometry detection, elisa, and western blot assay were performed to detect cell proliferation, apoptosis, and the release of proinflammatory cytokines. |
2018-05-29 |
2023-08-13 |
human |
| Jiaqi Wang, Yixuan Pan, Jiacheng Hu, Qiang Ma, Yi Xu, Yijian Zhang, Fei Zhang, Yingbin Li. Tea polyphenols induce S phase arrest and apoptosis in gallbladder cancer cells. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 51. issue 4. 2018-05-24. PMID:29513793. |
western blot assay was used to detect the expression of proteins related to cell cycle and apoptosis. |
2018-05-24 |
2023-08-13 |
mouse |
| Gaoxin Lei, Menglong Xu, Zhipan Xu, Lili Gu, Chenchen Lu, Zhengli Bai, Yue Wang, Yongbo Zhang, Huajing Hu, Yiwei Jiang, Wenfeng Zhao, Shuhua Ta. A Novel Fully Human Agonistic Single Chain Fragment Variable Antibody Targeting Death Receptor 5 with Potent Antitumor Activity In Vitro and In Vivo. International journal of molecular sciences. vol 18. issue 10. 2018-05-23. PMID:28953230. |
in addition, the activation of caspase-dependent apoptosis was evaluated by western blot assays. |
2018-05-23 |
2023-08-13 |
human |
| Seema Yousuf, Daniel J Brat, Hui-Kuo Shu, Ya Wang, Donald G Stein, Fahim Ati. Progesterone improves neurocognitive outcomes following therapeutic cranial irradiation in mice. Hormones and behavior. vol 96. 2018-05-22. PMID:28866326. |
western blot analysis of hippocampal and cortical tissue showed that ir at both doses induced astrocytic activation (glial fibrillary acidic protein), reactive macrophages/microglia (cd68) and apoptosis (cleaved caspase-3) and prog treatment inhibited these markers of brain injury. |
2018-05-22 |
2023-08-13 |
mouse |
| Xue-Juan Zan, Dong-Yun Rong, Yun-Hua Tu, Yue-Cui Xue, Zhen-Yuan Ye, Ying-Qian Kang, Ying Zhou, Yu Ca. [Effect of turmeric volatile oil on proliferation and apoptosis of human skin SCC A431 cells]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 41. issue 15. 2018-05-22. PMID:28914033. |
apoptosis was evaluated with ao/eb double staining and flow cytometry (fcm); cell cycle was analyzed with fcm ．western blot method was used to detect caspase-3 and caspase-9 protein expression. |
2018-05-22 |
2023-08-13 |
human |
| Feifan Xu, Yuanlong Han, Dandan Zhu, Hua Tian, Huiming Zhu, Jingjing Ren, Delin Gu, Yinong Dua. Construction of a recombinant pIRES2-EGFP-ARTS plasmid and its effect on LX-2 cells. Molecular medicine reports. vol 16. issue 4. 2018-05-21. PMID:28791356. |
in addition, the effects of pires2‑egfp‑arts on the activation, apoptosis, viability and migration of lx‑2 cells were assessed by western blot analysis, tunel staining, an mtt assay, and scratch and transwell assays, respectively. |
2018-05-21 |
2023-08-13 |
human |
| Chen Zhu, Nirong Bao, Shuo Chen, Jianning Zha. Dioscin enhances osteoblastic cell differentiation and proliferation by inhibiting cell autophagy via the ASPP2/NF-κβ pathway. Molecular medicine reports. vol 16. issue 4. 2018-05-21. PMID:28849197. |
western blot analysis showed that dioscin regulated autophagy‑associated protein expression in mc3t3‑e1 cells; it promoted the expression of apoptosis stimulated protein of p53‑2 (aspp2), and inhibited the expression of nuclear factor (nf)‑κβ and microtubule‑associated protein 1 light chain 3β, in a concentration‑dependent manner. |
2018-05-21 |
2023-08-13 |
Not clear |
| Iram Iqbal Hejazi, Rashmin Khanam, Syed Hassan Mehdi, Abdul Roouf Bhat, M Moshahid Alam Rizvi, Asimul Islam, Sonu Chand Thakur, Fareeda Atha. New insights into the antioxidant and apoptotic potential of Glycyrrhiza glabra L. during hydrogen peroxide mediated oxidative stress: An in vitro and in silico evaluation. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 94. 2018-05-18. PMID:28763750. |
apoptosis was established by dapi fluorescent staining and western blot analysis of pro apoptotic protein caspase-8, caspase-3 and anti-apoptotic protein bcl-2.there is an up regulation in the levels of pro apoptotic caspase-8 and caspase-3 and down regulation of anti-apoptotic bcl-2. |
2018-05-18 |
2023-08-13 |
human |
| Qiang Wan, Yu-Ping Yang, Zhong-Yong Li. [Puerarin attenuates PM2.5-induced vascular endothelial cells injury via ERK1/2 signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 41. issue 12. 2018-05-18. PMID:28901078. |
different concentrations of pm2.5 (0,20, 200, 400 mg•l⁻¹) were used to contaminate ea.hy926 cells for 24 h. the cells survival rate was detected by mtt assay; cells apoptosis of ea.hy926cells was detected by flow cytometry; the protein levels of p-erk1/2, bax and bcl-2 were detected by western blot; the contents of tumor necrosis factor-α (tnf-α), interleukin-6 (il-6), malonaldehyde (mda), and the activities of superoxide dismutase (sod) and lactic dehydrogenase (ldh) were measured by elisa. |
2018-05-18 |
2023-08-13 |
human |
| Kai Liu, Xinke Zhao, Jing Gu, Jianjun Wu, Hong Zhang, Yingdong L. Effects of 12C6+ heavy ion beam irradiation on the p53 signaling pathway in HepG2 liver cancer cells. Acta biochimica et biophysica Sinica. vol 49. issue 11. 2018-05-18. PMID:29036263. |
in order to explore whether the intrinsic or extrinsic pathway participates this process, hepg2 cells were treated with 12c6+ hib irradiation at doses of 0 (control), 1, 2, 4, and 6 gy with various methods employed to understand relevant mechanisms, such as detection of apoptosis, cell cycle, and fas expression by flow cytometry, analysis of apoptotic morphology by electron microscopy and laser scanning confocal microscopy, and screening differentially expressed genes relating to p53 signaling pathway by pcr-array assay following with any genes confirmed by western blot analysis. |
2018-05-18 |
2023-08-13 |
human |
| Tong-Huai Li, Jing-Jing Zhang, Shao-Xiao Liu, Yan Che. Long non-coding RNA taurine-upregulated gene 1 predicts unfavorable prognosis, promotes cells proliferation, and inhibits cells apoptosis in epithelial ovarian cancer. Medicine. vol 97. issue 19. 2018-05-15. PMID:29742691. |
ckk-8, annexin v-fitc-propidium iodide, qpcr and western blot assays were performed to detect cells proliferation, cells apoptosis, rna expression, and protein expression, respectively.lncrna tug1 expression was higher in tumor tissue compared to paired adjacent tissue (p < .001), and it was positively correlated with pathological grade (p = .022), tumor size (p = .011) and figo stage (p < .001). |
2018-05-15 |
2023-08-13 |
Not clear |
| Marzieh Moeinifard, Zuhair Mohammad Hassan, Faranak Fallahian, Maryam Hamzeloo-Moghadam, Mohammad Taghikhan. Britannin induces apoptosis through AKT-FOXO1 pathway in human pancreatic cancer cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 94. 2018-05-14. PMID:28821161. |
additionally, by using western blot and real-time pcr, we observed that britannin induced apoptosis by decreasing the expression of bcl-2 and increasing the expression of bax. |
2018-05-14 |
2023-08-13 |
human |