| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wenshuang Wu, Tao Wei, ZhiHui Li, Jingqiang Zh. p53-dependent apoptosis is essential for the antitumor effect of paclitaxel response to DNA damage in papillary thyroid carcinoma. International journal of medical sciences. vol 18. issue 14. 2021-08-18. PMID:34400889. |
rad-p53 + ptx increased the levels of cleaved-parp-1, cleaved -caspase 3, and bax and decreased the level of bcl-xl, suggesting p53 regulates the expression of bax/bcl-xl to mediate dna damage-induced apoptosis in ptc cells. |
2021-08-18 |
2023-08-13 |
Not clear |
| Lin Zhang, De-Yu Fang, Chun Liu, Dan-Yu Zhao, Yan-Jie Wang, Wen-Na Chen, Yong Cui, Jun-Fu Guo, Pei-Wei Cong, Xiao-Fan Feng, Yun-Ting Zhan. [Mechanism of puerarin reversing SH-SY5Y cell injury induced by Aβ_(1-42) based on proteomics]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 14. 2021-08-18. PMID:34402289. |
puerarin has the anti-alzheimer's disease (ad) activity,which can reverse nerve injury induced by aβand inhibit neuronal apoptosis.however,its potential pharmacodynamic mechanism still needs to be further researched.the occurrence and development of ad is due to the change of multiple metabolic links in the body,which leads to the destruction of balance.puerarin may act on multiple targets and multiple metabolic processes to achieve therapeutic purposes.quantitative proteomic analysis provides a new choice to understand the mechanism as completely as possible.this research adopted sh-sy5y cells induced by aβ_(1-42)to establish ad cell model,and aβimmunofluorescence detection showed that aβdecreased significantly after puerarin intervention.the mechanism of puerarin reversing sh-sy5y cell injured by aβ_(1-42)was further explored by using label-free non-labeled quantitative technology and western blot detection based on bioinformatics analysis result.the results showed that most of the differential proteins were related to biological processes such as cellular component organization or biogenesis,cellular component organization and cellular component biogenesis,and they mainly participated in the top ten pathways of p value such as pathogenic escherichia coli infection,m tor signaling pathway,regulation of autophagy,regulation of actin cytoskeleton,spliceosome,hepatocellular carcinoma,tight junction,non-small cell lung cancer,apoptosis and gap junction.annexin v/pi flow cytometry and tunel were used to detect apoptosis,and the results showed that aβdecreased significantly and the rate of apoptosis decreased significantly after puerarin intervention.western blot analysis found that the protein expression level of autophagy related protein lc3Ⅱwas up-regulated after aβinduction,and the degree of this up-regulation was further enhanced in puerarin intervention group.the trend of the ratio of lc3Ⅱ/lc3Ⅰamong groups was the same as the protein expression level of lc3Ⅱ，the protein expression level of p62 in the control group,ad model group and puerarin intervention group decreased successively.protein interaction network analysis showed that cap1 was correlated with tuba1b,hsp90ab2p,dnm1l,tuba1a and erk1/2,and the correlation between cap1 and erk1/2 was the highest among them.western blot showed that the expressions of p-erk1/2,bax and cap1 were significantly down-regulated and the protein expression level of bcl-2 was significantly up-regulated after puerarin intervention.therefore,puerarin might improve the sh-sy5y cells injured by aβ_(1-42)through the interaction of multiple biological processes and pathways in cells multiple locations,and cap1 might play an important role among them. |
2021-08-18 |
2023-08-13 |
Not clear |
| He Yu, Zhong-Mei He, Kun Shi, Yan Zhao, Ying Zong, Jian-Ming Li, Wei-Jia Chen, Rui D. [Study on antidepressant effect of total alkaloids of Fibraurea recisa]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 14. 2021-08-18. PMID:34402292. |
this study aimed to investigate the antidepressant effects of total alkaloids of fibraurea recisa in ht22 cells damaged by corticosterone (cort) in vitro and in a mouse model of chronic unpredictable mild stress (cums) as well as the underlying mechanisms.in cellular experiments,the viability of cort-damaged ht22 cells was detected using cell counting kit-8 (cck-8),and the cell apoptosis was detected by hoechst 33258 staining.in animal experiments,c57bl/6n mice were randomly divided into the control group,model group,low (100 mg·kg~(-1)),medium (200 mg·kg~(-1)) and high (400 mg·kg~(-1))-dose of total alkaloids of f.recisa groups,and positive control group.after 21 days of cums exposure,their depressive behaviors were observed in behavioral and morris water maze tests.the serum levels of 5-hydroxytryptamine (5-ht),dopamine (da),and norepinephrine (ne) were assessed by elisa.the expression levels of apoptosis-related proteins bcl-2,bax and cleaved caspase-3 in ht22 cells and mouse hippocampus were detected by western blot.the results suggested that total alkaloids of f.recisa alleviated the damage of ht22 cells induced by cort in a dose-dependent manner.the hoechst 33258 staining uncovered that total alkaloids of f.recisa better reduced the blue spots and inhibited cell apoptosis.the results of animal experiments showed that total alkaloids of f.recisa significantly improved the depression-like behaviors of mice and increased the serum levels of 5-ht,da and ne as compared with those in the model group.the western blot assays revealed a significant up-regulation of bcl-2 protein expression,but an obvious reduction in bax and cleaved caspase-3protein expression in the total alkaloids of f.recisa group.in conclusion,total alkaloids of f.recisa inhibited depression possibly by regulating the apoptosis-related protein expression or elevating the monoamine neurotransmitter levels in the brain. |
2021-08-18 |
2023-08-13 |
mouse |
| Di Wang, Xi Ming, Jinhuan Xu, Yi Xia. Circ_0009910 shuttled by exosomes regulates proliferation, cell cycle and apoptosis of acute myeloid leukemia cells by regulating miR-5195-3p/GRB10 axis. Hematological oncology. vol 39. issue 3. 2021-08-17. PMID:33969901. |
functionally, blocking circ_0009910 via small interfering rna (sirna) suppressed cell proliferation and cell cycle progression, but facilitated apoptosis rate of hl-60 and molm-13 cells, accompanied with lower b-cell lymphoma 2 (bcl-2) level and higher bcl-2-associated x protein (bax) level. |
2021-08-17 |
2023-08-13 |
human |
| Xue Bai, Qiaoyun Dong, Li Zhao, Yan Yao, Bo Wan. microRNA-106b-containing extracellular vesicles affect autophagy of neurons by regulating CDKN2B in Parkinson's disease. Neuroscience letters. vol 760. 2021-08-17. PMID:34216715. |
mir-106b shuttled by msc-derived evs increased neuronal survival, autophagy, lc3ii/lc3i ratio and bcl-2 protein expression, while inhibited neuronal apoptosis and bax expression in pd mice. |
2021-08-17 |
2023-08-13 |
mouse |
| Yucong Xue, Muqing Zhang, Miaomiao Liu, Yu Liu, Li Li, Xue Han, Zhenqing Sun, Li Ch. 8-Gingerol Ameliorates Myocardial Fibrosis by Attenuating Reactive Oxygen Species, Apoptosis, and Autophagy Frontiers in pharmacology. vol 12. 2021-08-17. PMID:34393792. |
8-gin also could lead to down-regulation of the activities of matrix metalloproteinases-9 (mmp-9), caspase-9, and bax protein, up-regulation of the activity of bcl-2 protein, and alleviation of cardiomyocyte apoptosis. |
2021-08-17 |
2023-08-13 |
mouse |
| Yong Zuo, Jinhong Xie, Xincheng Li, Yan Li, Anand Thirupathi, Jianhua Zhang, Peng Yu, Guofen Gao, Yanzhong Chang, Zhenhua Sh. Ferritinophagy-Mediated Ferroptosis Involved in Paraquat-Induced Neurotoxicity of Dopaminergic Neurons: Implication for Neurotoxicity in PD. Oxidative medicine and cellular longevity. vol 2021. 2021-08-17. PMID:34394837. |
meanwhile, bcl2, bax, and p-38 were involved in apoptosis induced by pq. |
2021-08-17 |
2023-08-13 |
mouse |
| Xiaohuan Ding, Jing Lv, Jia Luan, Jun Zhan. Calycosin may Alleviate Ang II-Induced Pro-proliferative Effects on Glomerular Mesangial Cells via Partially Inhibiting Autophagy and ERK Signaling Pathway. Biological & pharmaceutical bulletin. vol 43. issue 12. 2021-08-16. PMID:32999213. |
human glomerular mesangial cells (gmcs) were cultured and treated by ang ii and 0, 0.1, 1, or 10 µm calycosin, and the viability and proliferation of the cells were determined by methyl thiazolyl tetrazolium (mtt) and 5-ethynil-2'-deoxyuridine (edu) staining; moreover, the apoptosis of the cells was examined by flow cytometry assay; furthermore, the expression levels of extracellular signal-regulated kinase (erk), p-erk, anti-apoptotic factor bcl-2, as well as pro-apoptotic factor bax have been examined by western blot (wb) methods; finally, the expression of autophagic markers in each group was examined by wb and immunocytochemistry methods. |
2021-08-16 |
2023-08-13 |
human |
| Guanpeng Xu, Xi Li, Zhiyong Zhu, Huisheng Wang, Xizhuang Ba. Iron Overload Induces Apoptosis and Cytoprotective Autophagy Regulated by ROS Generation in Mc3t3-E1 Cells. Biological trace element research. vol 199. issue 10. 2021-08-16. PMID:33405076. |
cell apoptosis was examined using annexin v-fitc/pi staining with a flow cytometry, and levels of bax, bcl-2, cleaved caspase-3, and cleaved parp were evaluated with western blot. |
2021-08-16 |
2023-08-13 |
Not clear |
| Ana Patrícia Bastos Ferreira, Ana Paula Pimentel Cassilhas, Patrícia Moura, Pedro Augusto Sampaio Rocha-Filh. Intrinsic and Extrinsic Cell Apoptotic Pathways in Patients with HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis: A Systematic Review. Viral immunology. vol 34. issue 6. 2021-08-16. PMID:33470891. |
the descriptors used for this systematic literature review were: tropical paraparesis, proto-oncogenic protein c, bcl-2, bcl-x protein, bax protein, fas ligand (fasl) protein, fas receptor, tnf-related apoptosis-inducing ligand and fas-associated protein with death domain (fadd)-like apoptosis regulating. |
2021-08-16 |
2023-08-13 |
human |
| Haixia Zhao, Laixin Song, Na Ma, Chaoqi Liu, Yaoyan Dun, Zhiyong Zhou, Ding Yuan, Changcheng Zhan. The dynamic changes of Nrf2 mediated oxidative stress, DNA damage and base excision repair in testis of rats during aging. Experimental gerontology. vol 152. 2021-08-16. PMID:34175407. |
finally, tunel and western blot results further confirmed germ cell apoptosis progressively increased from 3 to 24 months of age as evidenced by decreased ratio of bcl-2/bax and levels of bcl-2 expression, and increased bax expression levels. |
2021-08-16 |
2023-08-13 |
rat |
| Marc Lebordais, Juan Manuel Gutierrez-Villagomez, Julien Gigault, Magalie Baudrimont, Valerie S Langloi. Molecular impacts of dietary exposure to nanoplastics combined with arsenic in Canadian oysters (Crassostrea virginica) and bioaccumulation comparison with Caribbean oysters (Isognomon alatus). Chemosphere. vol 277. 2021-08-16. PMID:34384184. |
the investigated gene functions were endocytosis (cltc), oxidative stress (gapdh, sod3, cat), mitochondrial metabolism (12s), cell cycle regulation (gadd45, p53), apoptosis (bax, bcl-2), detoxification (cyp1a, mdr, mt), and energy storage (vit). |
2021-08-16 |
2023-08-13 |
cat |
| Xuexia Liu, Qian Li, Zhixin Wang, Fujun Li. Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide. Journal of cellular and molecular medicine. vol 25. issue 3. 2021-08-15. PMID:33438283. |
the results of immunohistochemistry and western blot showed that cp reduced the proliferation of germ cells (pcna, plzf) and increased germ cell apoptosis (bax and tunel-positive cells) in cp-treated mice testes. |
2021-08-15 |
2023-08-13 |
mouse |
| Hao Li, Jingjing Shi, Haihang Gao, Xiaoqi Yang, Yang Fu, Yuxuan Peng, Ying Xia, Donghai Zho. Hexavalent Chromium Causes Apoptosis and Autophagy by Inducing Mitochondrial Dysfunction and Oxidative Stress in Broiler Cardiomyocytes. Biological trace element research. 2021-08-14. PMID:34390448. |
the results showed that, compared with the control group, reactive oxygen species (ros) and apoptosis rate in the cr(vi) treatment group increased in a dose-dependent manner, the mrna levels of apoptosis-related genes bax and p53 were significantly increased, and the mrna level of bcl-2 was significantly decreased. |
2021-08-14 |
2023-08-13 |
Not clear |
| Haiying Fu, Junjie Zhang, Mayu Huan. Topiroxostat ameliorates oxidative stress and inflammation in sepsis-induced lung injury. Zeitschrift fur Naturforschung. C, Journal of biosciences. vol 75. issue 11-12. 2021-08-13. PMID:32589612. |
the cell apoptosis in lung was detected by tunnel staining and western blot analysis of apoptosis-related proteins including pro-apoptosis proteins, bax, cleaved caspase9, cleaved caspase3 and anti-apoptosis protein bcl2. |
2021-08-13 |
2023-08-13 |
rat |
| Dejun Xu, Lingbin Liu, Yongju Zhao, Li Yang, Jianyong Cheng, Rongmao Hua, Zelin Zhang, Qingwang L. Melatonin protects mouse testes from palmitic acid-induced lipotoxicity by attenuating oxidative stress and DNA damage in a SIRT1-dependent manner. Journal of pineal research. vol 69. issue 4. 2021-08-13. PMID:32761924. |
moreover, melatonin therapy suppressed pa-induced apoptosis by modulating apoptosis-associated proteins such as bcl2, bax, c-caspase3, c-caspase12, and chop in type b spermatogonial stem cells. |
2021-08-13 |
2023-08-13 |
mouse |
| Xiaoli Gao, Xiaoyu Huang, Qiaoli Yang, Shengwei Zhang, Zunqiang Yan, Ruirui Luo, Pengfei Wang, Wei Wang, Kaihui Xie, Shuangbao Gu. MicroRNA-21-5p targets PDCD4 to modulate apoptosis and inflammatory response to Clostridium perfringens beta2 toxin infection in IPEC-J2 cells. Developmental and comparative immunology. vol 114. 2021-08-13. PMID:32888967. |
noteworthily, transfection of cpb2-treated ipec-j2 cells with mir-21-5p mimic increased cell viability and bcl2 expression, as well as reduced cytotoxicity, apoptosis rates and bax level. |
2021-08-13 |
2023-08-13 |
Not clear |
| Bruna Pasqualotto Costa, Marcella Tornquist Nassr, Fernando Mendonça Diz, Leonardo Pfeiff Carlessi, Krist Helen Antunes Fernandes, Fernanda Bordignon Nunes, Gisele Branchini, Jarbas Rodrigues de Oliveir. Fructose-1,6-bisphosphate induces generation of reactive oxygen species and activation of p53-dependent cell death in human endometrial cancer cells. Journal of applied toxicology : JAT. vol 41. issue 7. 2021-08-12. PMID:33078453. |
these harmful stimuli trigger the upregulation of the expression of pro-apoptotic genes (p53 and bax), leading to the reduction of cell proliferation through inducing programmed cell death by apoptosis. |
2021-08-12 |
2023-08-13 |
human |
| Zhiqing Shen, Aling Shen, Xiaoping Chen, Xiangyan Wu, Jianfeng Chu, Ying Cheng, Meizhong Peng, Youqin Chen, Nathaniel Weygant, Meizhu Wu, Xiaoying Lin, Jun Peng, Keji Che. Huoxin pill attenuates myocardial infarction-induced apoptosis and fibrosis via suppression of p53 and TGF-β1/Smad2/3 pathways. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 130. 2021-08-12. PMID:34321167. |
in support of these findings, hxp attenuated cardiac apoptosis and decreased p53 and bax protein expression, while increasing bcl-2 protein expression in cardiac tissues of mi mice. |
2021-08-12 |
2023-08-13 |
mouse |
| Shun Iida, Sohtaro Mine, Keiji Ueda, Tadaki Suzuki, Hideki Hasegawa, Harutaka Katan. Suberoyl bis-hydroxamic acid reactivates Kaposi's sarcoma-associated herpesvirus through histone acetylation and induces apoptosis in lymphoma cells. Journal of virology. 2021-08-11. PMID:33328303. |
in addition to its effect of kshv reactivation, this study revealed that sbha induces apoptosis in pel cells in a dose-dependent manner, inducing acetylation and phosphorylation of p53, cleavage of caspases, and expression of pro-apoptotic factors such as bim and bax. |
2021-08-11 |
2023-08-13 |
Not clear |