| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Y Y Gan, C M Lai, S H Lee, L H Ga. Production of monoclonal and polyclonal antibodies against various haemoglobins for the detection of thalassaemias. Annals of the Academy of Medicine, Singapore. vol 25. issue 1. 1996-09-19. PMID:8779551. |
the developed antibodies include the polyclonal antibodies against hb bart's and hb h; monoclonal antibodies (mab) against hb h, used in a sandwich enzyme-linked immunosorbent assay (elisa), for detecting carriers of (--sea/) deletion and deletions involving the complete zeta-alpha-globin gene cluster, such as (--alpha fil/), (--alpha thai/) and (--hw/), which are the common deletional alpha-thalassaemias in southeast asians; mab against zeta-globin chains used in an immunocytological test, for the detection of adult carriers of (--sea/) deletion except for (alpha 20.5/), (--alpha fil/) and (--alpha thai/) (this simple test is useful in identifying couples at risk of conceiving foetuses afflicted with the hb bart's hydrops foetalis syndrome due to homozygous alpha-thalassaemia); mab against hb a2 and beta- and gamma-globin chains used for the quantitation of hb a2 in beta-thalassaemia and the diagnosis of beta-thalassaemia major in foetuses respectively; other mabs produced to date include those specific to haemoglobins d-los angeles, j-baltimore, o-arab and j-paris-i. |
1996-09-19 |
2023-08-12 |
Not clear |
| A Y Chan, C K So, L C Cha. Comparison of the HbH inclusion test and a PCR test in routine screening for alpha thalassaemia in Hong Kong. Journal of clinical pathology. vol 49. issue 5. 1996-09-12. PMID:8707958. |
to compare the haemoglobin (hb) h inclusion test with a polymerase chain reaction (pcr) test in routine screening for alpha thalassaemia. |
1996-09-12 |
2023-08-12 |
Not clear |
| N S Smetanina, T H Huisma. MRNA analysis in reticulocytes of subjects with Hb D, Hb Porto Alegre, Hb E, and different types of unstable hemoglobin variants. American journal of hematology. vol 52. issue 4. 1996-08-30. PMID:8701943. |
using a reverse transcription-polymerase chain reaction (rt-pcr) technique we determined the alpha 2/alpha 1, alpha/beta, and gamma/beta mrna ratios in reticulocytes of 11 patients with seven different unstable beta chain variants, of 4 patients with two unstable alpha chain variants, in hemoglobin (hb) d, hb porto alegre, and hb e heterozygotes, and in 8 patients with hb x-beta 0-thalassemia (thal) (three d-beta 0-thal, one porto alegre = beta 0-thal, one lulu island-beta 0-thal, and three e-beta 0-thal). |
1996-08-30 |
2023-08-12 |
human |
| N S Smetanina, T H Huisma. MRNA analysis in reticulocytes of subjects with Hb D, Hb Porto Alegre, Hb E, and different types of unstable hemoglobin variants. American journal of hematology. vol 52. issue 4. 1996-08-30. PMID:8701943. |
the alpha/beta mrna ratio in four hb e heterozygotes averaged 4.21 (normal, 4.47), and that in 2 patients with hb e-beta 0-thal and four alpha-globin genes (alpha alpha/alpha alpha) averaged a high 22.4. |
1996-08-30 |
2023-08-12 |
human |
| S C Björling, R A Goldbeck, S J Paquette, S J Milder, D S Klige. Allosteric intermediates in hemoglobin. 1. Nanosecond time-resolved circular dichroism spectroscopy. Biochemistry. vol 35. issue 26. 1996-08-22. PMID:8679624. |
in the allosteric core model of hb cooperativity, fe-co bond breakage initiates a heme structural change, thought to be heme doming, that is transmitted to the alpha 1 beta 2 interface via the f helix. |
1996-08-22 |
2023-08-12 |
Not clear |
| R Petruzzelli, G Aureli, A Lania, A Galtieri, A Desideri, B Giardin. Diving behaviour and haemoglobin function: the primary structure of the alpha- and beta-chains of the sea turtle (Caretta caretta) and its functional implications. The Biochemical journal. vol 316 ( Pt 3). 1996-08-07. PMID:8670176. |
comparison with that of human hb shows differences in several residues involved in both alpha 1 beta 1 and alpha 1 beta 2 packing contacts. |
1996-08-07 |
2023-08-12 |
human |
| T Horiuchi, T Nakamura, H Tsutsumi, M Miyao, J Inoue, A Araki, A Hattori, A Harano, M Mori, H It. [A case of abnormal hemoglobin (HbJ Cape Town) with high serum levels of HbAlc]. Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics. vol 33. issue 2. 1996-07-26. PMID:8656577. |
subsequently, we sequenced isolated abnormal alpha chain and detected the substitution of ariginine for glutamamine at position 92 (hb j cape town). |
1996-07-26 |
2023-08-12 |
Not clear |
| B Vallone, A Bellelli, A E Miele, M Brunori, G Ferm. Probing the alpha 1 beta 2 interface of human hemoglobin by mutagenesis. Role of the FG-C contact regions. The Journal of biological chemistry. vol 271. issue 21. 1996-07-22. PMID:8647854. |
we have expressed and characterized one double and two single mutants: hb alpha t38w/beta w37t, hb beta w37t, and hb alpha t38w, whose structure has been determined by crystallography. |
1996-07-22 |
2023-08-12 |
human |
| H W Kim, T J Shen, N T Ho, M Zou, M F Tam, C H. Contributions of asparagine at alpha 97 to the cooperative oxygenation process of hemoglobin. Biochemistry. vol 35. issue 21. 1996-07-17. PMID:8639610. |
recombinant hb (alpha 97asn-->ala) shows a milder alteration of functional properties compared to the severely impaired beta 99 mutants of the human abnormal hemoglobins. |
1996-07-17 |
2023-08-12 |
human |
| H W Kim, T J Shen, N T Ho, M Zou, M F Tam, C H. Contributions of asparagine at alpha 97 to the cooperative oxygenation process of hemoglobin. Biochemistry. vol 35. issue 21. 1996-07-17. PMID:8639610. |
the addition of inositol hexaphosphate, an allosteric effector, causes recovery of the functional properties of recombinant hb (alpha 97 asn-->ala) almost to the level of human normal adult hemoglobin without this allosteric effector. |
1996-07-17 |
2023-08-12 |
human |
| H W Kim, T J Shen, N T Ho, M Zou, M F Tam, C H. Contributions of asparagine at alpha 97 to the cooperative oxygenation process of hemoglobin. Biochemistry. vol 35. issue 21. 1996-07-17. PMID:8639610. |
r hb (alpha 97 asn-->ala) shows very similar tertiary structure around the heme pockets and quaternary structure in the alpha 1 beta 2 interface compared to those of human normal adult hemoglobin. |
1996-07-17 |
2023-08-12 |
human |
| H W Kim, T J Shen, N T Ho, M Zou, M F Tam, C H. Contributions of asparagine at alpha 97 to the cooperative oxygenation process of hemoglobin. Biochemistry. vol 35. issue 21. 1996-07-17. PMID:8639610. |
preliminary molecular dynamics simulations have been used to calculate the contributions of specific interactions of several amino acid residues in r hb (alpha 97asn-->ala) to the free energy of cooperativity of this recombinant hemoglobin. |
1996-07-17 |
2023-08-12 |
human |
| W E Rodriguez Romero, M Castillo, M A Chaves, G F Saenz, L H Gu, J B Wilson, E Baysal, N S Smetanina, J Y Leonova, T H Huisma. Hb Costa Rica or alpha 2 beta 2 77(EF1)His --> Arg: the first example of a somatic cell mutation in a globin gene. Human genetics. vol 97. issue 6. 1996-07-12. PMID:8641705. |
hb costa rica or alpha 2 beta 2 77(ef1)his --> arg: the first example of a somatic cell mutation in a globin gene. |
1996-07-12 |
2023-08-12 |
Not clear |
| M Coletta, M Angeletti, G De Sanctis, L Cerroni, B Giardina, G Amiconi, P Ascenz. Kinetic evidence for the existence of a rate-limiting step in the reaction of ferric hemoproteins with anionic ligands. European journal of biochemistry. vol 235. issue 1-2. 1996-07-03. PMID:8631366. |
the kinetics of azide and fluroide binding to various monomeric and tetrameric ferric hemoproteins (sperm whale mb, isolated alpha and beta chains of human hb reacted with p-chloromercuribenzoate, dromeday, ox and human hb) has been investigated (at ph 6.5 and 20 degrees c over a large range (20 microm to 2 m) of ligand concentration. |
1996-07-03 |
2023-08-12 |
human |
| M Marta, M Patamia, A Lupi, M Antenucci, M Di Iorio, S Romeo, R Petruzzelli, M Pomponi, B Giardin. Bovine hemoglobin cross-linked through the beta chains: functional and structural aspects. The Journal of biological chemistry. vol 271. issue 13. 1996-07-03. PMID:8631776. |
the reaction of bovine hb with nfplp leads to a cross-linkage between the beta subunits, which greatly stabilizes the low affinity t state of the molecule and simultaneously abolishes the tendency of the tetramer to dissociate into alpha beta dimers. |
1996-07-03 |
2023-08-12 |
cattle |
| Y Huang, T Yonetani, A Tsuneshige, B M Hoffman, G K Acker. Heterometallic hybrids of homometallic human hemoglobins. Proceedings of the National Academy of Sciences of the United States of America. vol 93. issue 9. 1996-07-01. PMID:8633083. |
it was found that four of the metal-substituted hbs (mg2+ hb, mn2+ hb, ni2+ hb, and zn2+ hb) did not form detectable amounts of heterometallic hybrids with normal fe2+ hb even though (i) their homometallic parents formed tight tetrameric complexes with stabilities similar to that of fe2+ hb and (ii) hybrids with metal substitution at both alpha sites or both beta sites are known to form readily. |
1996-07-01 |
2023-08-12 |
human |
| P Winichagoon, S Fucharoen, P Wilairat, Y Fukumak. Molecular mechanisms of thalassemia in southeast Asia. The Southeast Asian journal of tropical medicine and public health. vol 26 Suppl 1. 1996-06-26. PMID:8629113. |
hb constant spring (cs) occurs from the mutation at the termination codon of the alpha-globin gene resulting in an elongated polypeptide; alpha(cs)-globin mrna is also unstable and only small amounts of hb cs are produced. |
1996-06-26 |
2023-08-12 |
Not clear |
| J Tan, J S Tay, Y C Wong, S K Kham, N Bte Abd Aziz, S H Teo, H B Won. Molecular analysis of Hb Q-H disease and Hb Q-Hb E in a Singaporean family. The Southeast Asian journal of tropical medicine and public health. vol 26 Suppl 1. 1996-06-26. PMID:8629117. |
hb q (alpha 74asp-his) results from a mutation in the alpha-gene such that abnormal alpha q-chains are synthesized. |
1996-06-26 |
2023-08-12 |
Not clear |
| J Tan, J S Tay, Y C Wong, S K Kham, N Bte Abd Aziz, S H Teo, H B Won. Molecular analysis of Hb Q-H disease and Hb Q-Hb E in a Singaporean family. The Southeast Asian journal of tropical medicine and public health. vol 26 Suppl 1. 1996-06-26. PMID:8629117. |
the alpha q-chains combine with the normal beta a-chains to form abnormal hb alpha 2q beta 2a (hb q). |
1996-06-26 |
2023-08-12 |
Not clear |
| J Tan, J S Tay, Y C Wong, S K Kham, N Bte Abd Aziz, S H Teo, H B Won. Molecular analysis of Hb Q-H disease and Hb Q-Hb E in a Singaporean family. The Southeast Asian journal of tropical medicine and public health. vol 26 Suppl 1. 1996-06-26. PMID:8629117. |
cellulose acetate electrophoresis showed hb h and hb q with the absence of hb a. globin chain biosynthesis was carried out and alpha q- and beta-chains were detected. |
1996-06-26 |
2023-08-12 |
Not clear |