| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| C Ho, B F Willis, T J Shen, N T Dazhen, D P Sun, M F Tam, S M Suzuka, M E Fabry, R L Nage. Roles of alpha 114 and beta 87 amino acid residues in the polymerization of hemoglobin S: implications for gene therapy. Journal of molecular biology. vol 263. issue 3. 1996-12-18. PMID:8918602. |
three novel recombinant mutants of sickle hemoglobin (hb s, beta 6glu-->val) have been constructed to assess the role of proline at alpha 114 and threonine at beta 87 in the polymerization of deoxygenated hb s. using the hemoglobin expression system (phe2) designed in our laboratory, four plasmids were expressed separately in escherichia coli to produce the four recombinant hemoglobins: r hb s (beta 6glu-->val); r hb s-chiapas (beta 6glu-->val, alpha 114pro-->arg); r hb s-d-ibadan (beta 6glu-->val, beta 87thr-->lys); and r hb s-chiapas-d-ibadan (beta 6glu-->val, alpha 114pro-->arg, beta 87thr-->lys). |
1996-12-18 |
2023-08-12 |
human |
| C Ho, B F Willis, T J Shen, N T Dazhen, D P Sun, M F Tam, S M Suzuka, M E Fabry, R L Nage. Roles of alpha 114 and beta 87 amino acid residues in the polymerization of hemoglobin S: implications for gene therapy. Journal of molecular biology. vol 263. issue 3. 1996-12-18. PMID:8918602. |
the recombinant hemoglobins were further investigated by measuring the oxygen-binding properties, which were found to be comparable to those of hb a. delay-time gelation studies of the three mutants of r hb s were carried out in 1.8 m potassium phosphate (ph 7.34) by a temperature jump from 4 degrees c to 30 degrees c and an increase in delay time over that of r hb s was observed, as well as an overall decrease in the polymerization of these three mutants of hb s. a more detailed and quantitative investigation has also been carried out to determine the equilibrium solubility (csat) in 0.1 m potassium phosphate (ph 7.35) at 25 degrees c of the three hb s mutants as well as of mixtures of these mutants with hb s versus mixtures of fetal hemoglobin (hb f) and hb a with hb s. the inhibition of polymerization demonstrated in these experiments suggests that the interactions involving the two amino acid residues alpha 114pro and beta 87thr are very important to the formation of hb s polymer, and modification of these amino acids results in an anti-sickling potential. |
1996-12-18 |
2023-08-12 |
human |
| T Yamaguchi, J Pang, K S Reddy, H E Witkowska, S Surrey, K Adach. Expression of soluble human beta-globin chains in bacteria and assembly in vitro with alpha-globin chains. The Journal of biological chemistry. vol 271. issue 43. 1996-12-16. PMID:8900144. |
these results indicate that presence of alpha chains favors assembly with beta-globin, beta-beta dimers cannot bind alpha chains, and that hb a tetramer formation results in the most thermally stable species. |
1996-12-16 |
2023-08-12 |
human |
| A S Moore, N G Coldham, M J Saue. A cellular mechanism for imidocarb retention in edible bovine tissues. Toxicology letters. vol 87. issue 2-3. 1996-12-12. PMID:8914612. |
binding capacities of selected bovine macromolecules for [14c]imidocarb were in the order deoxy-ribonucleic acid (dna) = ribonucleic acid (rna) > > alpha 1-acid glycoprotein (agp) > serum albumin (bsa) > haemoglobin (hb). |
1996-12-12 |
2023-08-12 |
cattle |
| P Pannangpetch, O L Woodma. The effect of ischaemia on endothelium-dependent vasodilatation and adrenoceptor-mediated vasoconstriction in rat isolated hearts. British journal of pharmacology. vol 117. issue 6. 1996-12-11. PMID:8882595. |
inhibition of the action of no by hb significantly enhanced the vasoconstrictor response to the non-selective alpha-adrenoceptor agonist, noradrenaline (na, 0.1-10 nmol) and the alpha 2-adrenoceptor agonist, b-ht 920 (0.001-1 mumol) but had no effect on the vascular response to the alpha 1-adrenoceptor agonist, methoxamine (mtx, 10-300 nmol). |
1996-12-11 |
2023-08-12 |
rat |
| P Pannangpetch, O L Woodma. The effect of ischaemia on endothelium-dependent vasodilatation and adrenoceptor-mediated vasoconstriction in rat isolated hearts. British journal of pharmacology. vol 117. issue 6. 1996-12-11. PMID:8882595. |
enhanced constrictor responses to noradrenaline and b-ht 920 in the presence of hb indicates that endogenous no modulates the constriction of coronary resistance vessels in response to stimulation of alpha 2-adrenoceptors. |
1996-12-11 |
2023-08-12 |
rat |
| T MIYAJI, I IUCHI, S SHIBATA, I TAKEDA, A TAMUR. POSSIBLE AMINO ACID SUBSTITUTION IN THE ALPHA CHAIN (ALPHA-87TYR) OF HB M-IWATE. Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society. vol 26. 1996-12-01. PMID:14095683. |
possible amino acid substitution in the alpha chain (alpha-87tyr) of hb m-iwate. |
1996-12-01 |
2023-08-12 |
Not clear |
| S Ayala, D Colomer, A Pujades, M Aymerich, J L Vives Corron. Haemoglobin Lleida: a new alpha 2-globin variant (12 bp deletion) with mild thalassaemic phenotype. British journal of haematology. vol 94. issue 4. 1996-11-20. PMID:8826886. |
since the deletion affects an aminoacid residue (114 pro) involved in alpha 1-beta 1-globin chain contacts, the interaction required for efficient hb assembly is also compromised. |
1996-11-20 |
2023-08-12 |
Not clear |
| M Tamburrini, R D'Avino, A Fago, V Carratore, A Kunzmann, G Prisc. The unique hemoglobin system of Pleuragramma antarcticum, an antarctic migratory teleost. Structure and function of the three components. The Journal of biological chemistry. vol 271. issue 39. 1996-11-18. PMID:8798605. |
hb 1 has the alpha chain in common with hb 2 and the beta in common with hb 3. |
1996-11-18 |
2023-08-12 |
human |
| C L Harteveld, P C Giordano, M Losekoot, J G Heister, D Batelaan, P van Delft, M C Bruin, L F Bernin. Hb Utrecht [alpha 2 129(H12)Leu-->Pro], a new unstable alpha 2-chain variant associated with a mild alpha-thalassaemic phenotype. British journal of haematology. vol 94. issue 3. 1996-11-01. PMID:8790146. |
hb utrecht [alpha 2 129(h12)leu-->pro], a new unstable alpha 2-chain variant associated with a mild alpha-thalassaemic phenotype. |
1996-11-01 |
2023-08-12 |
Not clear |
| H E Witkowska, B N Green, M Morris, C H Shackleto. Intact protein electrospray ionization tandem mass spectrometry can be the sole technique used for confirming the structure of a variant hemoglobin. Rapid communications in mass spectrometry : RCM. vol Spec No. 1996-10-15. PMID:8829475. |
examination of low-abundance product ions allowed the unequivocal identification of the mutation as alpha 136 leu --> met, a hemoglobin previously named hb chicago. |
1996-10-15 |
2023-08-12 |
human |
| M J Peres, L Romão, H Carreiro, I Picanço, L Batalha, H A Magalhães, M C Martins, J Lavinh. Molecular basis of alpha-thalassemia in Portugal. Hemoglobin. vol 19. issue 6. 1996-10-11. PMID:8718693. |
in a group of 342 subjects presenting beta-thalassemia, or hb s trait, beta-thalassemia major sickle cell disease or low red blood cell indices, the -alpha 3.7, -alpha 4.2, -sea, -med, (alpha alpha)mm, and alpha alpha alpha anti 3.7 haplotypes were found in different combinations. |
1996-10-11 |
2023-08-12 |
human |
| T Harano, K Harano, S Uehara, K Matsushit. Two new alpha chain variants: Hb Fuchu-I [alpha 72(EF1)His-->Tyr] and Hb Fuchu-II [alpha 97(G4)Asn-->His]. Hemoglobin. vol 19. issue 6. 1996-10-11. PMID:8718697. |
two new alpha chain variants: hb fuchu-i [alpha 72(ef1)his-->tyr] and hb fuchu-ii [alpha 97(g4)asn-->his]. |
1996-10-11 |
2023-08-12 |
Not clear |
| Y Igarashi, S Matsuzaki, N Kanou, S Inami, T Nakamura, K Kasai, K Fushitan. The first case of Hb E-Saskatoon [alpha 2 beta(2)22(B4)Glu-->Lys] in a Japanese male in Asia. Hemoglobin. vol 19. issue 6. 1996-10-11. PMID:8718699. |
the first case of hb e-saskatoon [alpha 2 beta(2)22(b4)glu-->lys] in a japanese male in asia. |
1996-10-11 |
2023-08-12 |
Not clear |
| J M de Pablos Gallego, L H Gu, Leonova JYe, T H Huisma. Hb F-Veleta or alpha 2 G gamma(2)40(C6)Arg-->Gly. Hemoglobin. vol 19. issue 6. 1996-10-11. PMID:8718700. |
hb f-veleta or alpha 2 g gamma(2)40(c6)arg-->gly. |
1996-10-11 |
2023-08-12 |
Not clear |
| B W Day, R Jin, M H Karo. In vivo and in vitro reactions of toluene diisocyanate isomers with guinea pig hemoglobin. Chemical research in toxicology. vol 9. issue 3. 1996-10-10. PMID:8728499. |
ionspray ms analysis of the hb isolated from guinea pigs exposed in vivo to 2,4-tdi indicated carbamoylation products with both the alpha and beta chains in which one of the two original isocyanato groups had been hydrolyzed to the amine. |
1996-10-10 |
2023-08-12 |
Not clear |
| A Dumoulin, L Kiger, N Griffon, C Vasseur, I Kister, P Génin, M C Marden, J Pagnier, C Poyar. Two mutations in recombinant Hb beta F41(C7)Y, K82 (EF6)D show additive effects in decreasing oxygen affinity. Protein science : a publication of the Protein Society. vol 5. issue 1. 1996-10-09. PMID:8771203. |
functional studies have shown that the hb alpha 2 beta 2(c7)f41y exhibits a decreased oxygen affinity relative to hb a, without a significantly increased autooxidation rate. |
1996-10-09 |
2023-08-12 |
Not clear |
| N S Smetanina, C Oner, E Baysal, R Oner, G Bozkurt, C Altay, A Gürgey, A D Adekile, L H Gu, T H Huisma. The relative levels of alpha 2-, alpha 1-, and zeta-mRNA in HB H patients with different deletional and nondeletional alpha-thalassemia determinants. Biochimica et biophysica acta. vol 1316. issue 3. 1996-10-04. PMID:8781536. |
the relative levels of alpha 2-, alpha 1-, and zeta-mrna in hb h patients with different deletional and nondeletional alpha-thalassemia determinants. |
1996-10-04 |
2023-08-12 |
Not clear |
| N S Smetanina, C Oner, E Baysal, R Oner, G Bozkurt, C Altay, A Gürgey, A D Adekile, L H Gu, T H Huisma. The relative levels of alpha 2-, alpha 1-, and zeta-mRNA in HB H patients with different deletional and nondeletional alpha-thalassemia determinants. Biochimica et biophysica acta. vol 1316. issue 3. 1996-10-04. PMID:8781536. |
we have analyzed the alpha 2/alpha 1-, alpha/beta-, zeta/(alpha + zeta)-mrna ratios in the retic-ulocytes of 40 patients with hb h disease. |
1996-10-04 |
2023-08-12 |
Not clear |
| N S Smetanina, C Oner, E Baysal, R Oner, G Bozkurt, C Altay, A Gürgey, A D Adekile, L H Gu, T H Huisma. The relative levels of alpha 2-, alpha 1-, and zeta-mRNA in HB H patients with different deletional and nondeletional alpha-thalassemia determinants. Biochimica et biophysica acta. vol 1316. issue 3. 1996-10-04. PMID:8781536. |
21 patients had deletional hb h disease (- -/- alpha), namely combinations of one of four types of alpha-thal-1 (med-i, med-ii, -(alpha)20.5, sea) and one of two types of alpha-thal-2 (-3.7 or -4.2 kb); 13 had hb h disease because of combinations of one of these alpha-thal-1 deletions with either a 5 nt deletion at the 5' splicing site of ivs-i, or a terminating codon mutation (hb cs), or a poly(a) mutation, and six were homozygous for either a poly(a) mutation or the 5 nt deletion. |
1996-10-04 |
2023-08-12 |
Not clear |