| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| J van den Heuvel, V Lang, G Richter, N Price, L Peacock, C Proud, J E McCarth. The highly acidic C-terminal region of the yeast initiation factor subunit 2 alpha (eIF-2 alpha) contains casein kinase phosphorylation sites and is essential for maintaining normal regulation of GCN4. Biochimica et biophysica acta. vol 1261. issue 3. 1995-06-15. PMID:7742363. |
a triple ser-->ala mutant form of yeast eif-2 alpha was found to be no longer phosphorylated by either of the yeast (or mammalian) casein kinase activities in vitro. |
1995-06-15 |
2023-08-12 |
Not clear |
| H R Mott, B S Baines, R M Hall, R M Cooke, P C Driscoll, M P Weir, I D Campbel. The solution structure of the F42A mutant of human interleukin 2. Journal of molecular biology. vol 247. issue 5. 1995-05-25. PMID:7723044. |
mutation of residue phe42 to ala abolishes binding to the alpha chain of the tri-partite il-2 receptor. |
1995-05-25 |
2023-08-12 |
human |
| J Ylänne, J Huuskonen, T E O'Toole, M H Ginsberg, I Virtanen, C G Gahmber. Mutation of the cytoplasmic domain of the integrin beta 3 subunit. Differential effects on cell spreading, recruitment to adhesion plaques, endocytosis, and phagocytosis. The Journal of biological chemistry. vol 270. issue 16. 1995-05-23. PMID:7721884. |
we further found that substitution of tyr747 with ala also abolished alpha iib beta a-mediated cell spreading. |
1995-05-23 |
2023-08-12 |
Not clear |
| A Yalçin, F Avcu, C Beyan, A Gürgey, A U Ura. A case of HB J-Meerut (or Hb J-Birmingham) [alpha 120(H3)Ala-->Glu]. Hemoglobin. vol 18. issue 6. 1995-05-16. PMID:7713747. |
a case of hb j-meerut (or hb j-birmingham) [alpha 120(h3)ala-->glu]. |
1995-05-16 |
2023-08-12 |
Not clear |
| H Wajcman, M de Montalembert, R Girot, J Kister, M Maier-Redelsperger, F Galactero. The use of Staphylococcus V8 protease in the structural determination of human hemoglobin variants: HB Valparaiso [alpha 88(F9)Ala-->Gly] example. Hemoglobin. vol 18. issue 4-5. 1995-03-14. PMID:7852082. |
the use of staphylococcus v8 protease in the structural determination of human hemoglobin variants: hb valparaiso [alpha 88(f9)ala-->gly] example. |
1995-03-14 |
2023-08-12 |
human |
| H Wajcman, M de Montalembert, R Girot, J Kister, M Maier-Redelsperger, F Galactero. The use of Staphylococcus V8 protease in the structural determination of human hemoglobin variants: HB Valparaiso [alpha 88(F9)Ala-->Gly] example. Hemoglobin. vol 18. issue 4-5. 1995-03-14. PMID:7852082. |
we report here on hb valparaiso [alpha 88(f9)ala-->gly], a new neutral variant with a slight increase in oxygen affinity, that was identified by use of this strategy. |
1995-03-14 |
2023-08-12 |
human |
| T Kamata, W Puzon, Y Takad. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29) The Biochemical journal. vol 305 ( Pt 3). 1995-03-08. PMID:7531439. |
chinese hamster ovary (cho) cells expressing beta 1 (d130a) (asp-130 to ala mutant of beta 1) and alpha 4 showed much less binding to both ligands than cho cells expressing wild-type beta 1 and alpha 4 [a dominant negative effects of beta 1 (d130a)], suggesting that asp-130 of beta 1 is critical for binding to both ligands and that the two ligand share common binding mechanisms [corrected]. |
1995-03-08 |
2023-08-12 |
Not clear |
| P Spencer, P M Jorda. Characterization of the two 5-aminolaevulinic acid binding sites, the A- and P-sites, of 5-aminolaevulinic acid dehydratase from Escherichia coli. The Biochemical journal. vol 305 ( Pt 1). 1995-02-16. PMID:7826323. |
the affinity for ala at the a-site of mg alpha zn beta alad was found to determine the km for the reaction and was ph-dependent, with its affinity increasing from 1 mm at ph 6 to 70 microm at ph 8.5. |
1995-02-16 |
2023-08-12 |
Not clear |
| P Spencer, P M Jorda. Characterization of the two 5-aminolaevulinic acid binding sites, the A- and P-sites, of 5-aminolaevulinic acid dehydratase from Escherichia coli. The Biochemical journal. vol 305 ( Pt 1). 1995-02-16. PMID:7826323. |
the affinity of ala at the p-site of zn alpha an beta alad is proposed to limit the km at ph values above 7, since the measured kd for ala at the a-site in 45 microm tris, ph 8, was well below the observed km (600 microm) under the same conditions. |
1995-02-16 |
2023-08-12 |
Not clear |
| R D Mitchell, D B Glass, C W Wong, K L Angelos, D A Wals. Heat-stable inhibitor protein derived peptide substrate analogs: phosphorylation by cAMP-dependent and cGMP-dependent protein kinases. Biochemistry. vol 34. issue 2. 1995-02-13. PMID:7819246. |
although the inhibitory peptide corresponding to this sequence (i.e., with an ala at position 21) is a much more potent inhibitor of pka than of pkg (approximately 250-fold), pkg actually exhibits a 60% higher kcat than does pka with the (ser21)pki alpha(14-22) amide substrate peptide, with only a 20-fold higher km value. |
1995-02-13 |
2023-08-12 |
Not clear |
| M F White, J Vasquez, S F Yang, J F Kirsc. Expression of apple 1-aminocyclopropane-1-carboxylate synthase in Escherichia coli: kinetic characterization of wild-type and active-site mutant forms. Proceedings of the National Academy of Sciences of the United States of America. vol 91. issue 26. 1995-02-02. PMID:7809054. |
the lys-273 to ala mutant has no detectable activity, supporting the identification of this residue as the base catalyzing c alpha proton abstraction. |
1995-02-02 |
2023-08-12 |
Not clear |
| M E Kennedy, L E Limbir. Palmitoylation of the alpha 2A-adrenergic receptor. Analysis of the sequence requirements for and the dynamic properties of alpha 2A-adrenergic receptor palmitoylation. The Journal of biological chemistry. vol 269. issue 50. 1995-01-12. PMID:7989367. |
previous studies have demonstrated that the alpha 2a-adrenergic receptor (alpha 2a ar) incorporates [3h]palmitate and that replacement of cys442 by ala or ser eliminates detectable acylation without perturbing coupling to pertussis toxin-sensitive gtp-binding proteins (kennedy, m. e., and limbird, l. e. (1993) j. biol. |
1995-01-12 |
2023-08-12 |
Not clear |
| M Gidh-Jain, Y Zhang, P D van Poelje, J Y Liang, S Huang, J Kim, J T Elliott, M D Erion, S J Pilkis, M Raafat el-Maghrab. The allosteric site of human liver fructose-1,6-bisphosphatase. Analysis of six AMP site mutants based on the crystal structure. The Journal of biological chemistry. vol 269. issue 44. 1994-11-29. PMID:7961695. |
thus, the met177 --> ala mutant suggests that the side chain beyond c alpha is not needed for amp binding, and that the leu30 --> phe mutant preserves the amp contacts with these side chains. |
1994-11-29 |
2023-08-12 |
human |
| C Fronticelli, M Gattoni, A L Lu, W S Brinigar, J L Bucci, E Chiancon. The dimer-tetramer equilibrium of recombinant hemoglobins. Stabilization of the alpha 1 beta 2 interface by the mutation beta(Cys112-->Gly) at the alpha 1 beta 1 interface. Biophysical chemistry. vol 51. issue 1. 1994-09-16. PMID:8061226. |
in a mutant at the alpha 1 beta 2 interface, beta(cys93-->ala), the association constant was decreased three-fold. |
1994-09-16 |
2023-08-12 |
human |
| D Y Kwo. The stabilizing effects of hydrophobic cores on peptide folding of bovine-pancreatic-trypsin-inhibitor folding-intermediate model. European journal of biochemistry. vol 223. issue 2. 1994-09-13. PMID:7519984. |
using this model the roles of hydrophobic cores, such as non-polar amino acids and a short beta strand, in the folding structure stability were investigated by deleting the hydrophobic amino acids or substituting with ala. as a first step, a mutant peptide, p alpha 6 p beta 1, was made by removing the four residues (nnfk46) from the n-terminus of p alpha which make a short beta strand in native bpti, and each of the hydrophobic cores, phe45 of p alpha and tyr21 of p beta, were substituted by ala. |
1994-09-13 |
2023-08-12 |
cattle |
| L M Mayr, D Willbold, P Rösch, F X Schmi. Generation of a non-prolyl cis peptide bond in ribonuclease T1. Journal of molecular biology. vol 240. issue 4. 1994-08-18. PMID:8035456. |
this conformation is demonstrated by the presence of a noesy cross-peak in the nmr spectrum between the c alpha protons of tyr38 and ala39 in the pro39-->ala variant. |
1994-08-18 |
2023-08-12 |
Not clear |
| K Adachi, P Konitzer, C G Paulraj, S Surre. Role of Leu-beta 88 in the hydrophobic acceptor pocket for Val-beta 6 during hemoglobin S polymerization. The Journal of biological chemistry. vol 269. issue 26. 1994-07-29. PMID:8021253. |
ala and phe substitutions at the beta 88 position in hb s were made using a yeast expression system in an effort to clarify the role of leu-beta 88 in creating a suitable acceptor site for val-beta 6 during polymerization of hb s. both ala- and phe-beta 88 substitutions in hb s inhibited polymerization compared with hb s. critical concentrations for polymerization of alpha 2 beta 2 val-6,ala-88 and alpha 2 beta 2val-6,phe-88 were 6- and 10-fold higher, respectively, than that of hb s (alpha 2 beta 2val-6,leu-88). |
1994-07-29 |
2023-08-12 |
Not clear |
| M A Devi, N P Da. Antiproliferative effect of polyunsaturated fatty acids and interleukin-2 on normal and abnormal human lymphocytes. Experientia. vol 50. issue 5. 1994-06-28. PMID:8194587. |
the polyunsaturated fatty acids (pufas), linoleic acid (la), alpha linolenic acid (ala), gamma linolenic acid (gla), arachidonic acid (aa), docosahexaenoic acid (dha) and eicosapentaenoic acid (epa), showed inhibition of growth of both normal and abnormal (molt-4) human lymphocytes, and inhibition was concentration-dependent. |
1994-06-28 |
2023-08-12 |
human |
| S Mukherji, T R Soderlin. Regulation of Ca2+/calmodulin-dependent protein kinase II by inter- and intrasubunit-catalyzed autophosphorylations. The Journal of biological chemistry. vol 269. issue 19. 1994-06-22. PMID:8188649. |
in this study inactive cam kinase ii beta subunit, generated by mutation of lys43 to ala, and active kinase alpha subunit were expressed separately (homomeric kinases) or co-expressed (heteromeric kinase) using the baculovirus/sf9 cell expression system and purified on cam-sepharose. |
1994-06-22 |
2023-08-12 |
Not clear |
| L K Lan. Functional expression of rat alpha 1 Na,K-ATPase containing substitutions for cysteines 454, 458, 459, 513 and 551. Biochemistry and molecular biology international. vol 31. issue 5. 1994-04-22. PMID:8136699. |
site-specific mutagenesis was used to prepare the following substitutions of the rat alpha 1 na,k-atpase: cys 513-->ala, cys 454+458+459-->ala and cys 551-->ser. |
1994-04-22 |
2023-08-12 |
rat |