| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| A Stea, S J Dubel, T P Snutc. alpha 1B N-type calcium channel isoforms with distinct biophysical properties. Annals of the New York Academy of Sciences. vol 868. 1999-08-12. PMID:10414290. |
utilizing chimeric alpha 1b-i and alpha 1b-ii cdnas, we show that: (1) the glu 177 to gly substitution in domain i s3 increases the rate of activation by approximately 15-fold; (2) the presence or absence of ala 415 in the domain i-ii linker alters current-voltage relations by approximately 10 mv but does not affect channel kinetics; (3) the substitution of gly 387 to glu in the domain i-ii linker also has no effect on kinetics; and (4) the presence or absence of sfmg (1236-1239) in domain iii s3-s4 did not significantly affect channel current-voltage relations, kinetics, or steady state inactivation. |
1999-08-12 |
2023-08-12 |
rat |
| M Padma, U N Da. Effect of cis-unsaturated fatty acids on the activity of protein kinases and protein phosphorylation in macrophage tumor (AK-5) cells in vitro. Prostaglandins, leukotrienes, and essential fatty acids. vol 60. issue 1. 1999-07-29. PMID:10319918. |
this cytotoxic action of c-ufas was found to be associated with an increase in free radical generation and lipid peroxidation process and a simultaneous decrease in cellular anti-oxidants such as superoxide dismutase (sod), catalase, glutathione peroxidase, glutathione reductase, glutathione and vitamin e. in the present study, it was observed that c-ufas such as gamma linolenic acid (gla), arachidonic acid (aa), eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) can activate phospholipase c (plc) and enhance diacylglycerol formation; all the fatty acids except alpha linolenic acid (ala) increased the binding of phorbol dibutyrate acetate (pdbu) suggesting translocation of protein kinase c (pkc) and at the same time these fatty acids (especially gla, aa, epa and dha) also enhanced pkc activity. |
1999-07-29 |
2023-08-12 |
Not clear |
| S Deutsch, R Darbellay, R Offord, A Frutiger, J Kister, H Wajcman, P Beri. Hb Iraq-Halabja beta10 (A7) Ala-->Val (GCC-->GTC): a new beta-chain silent variant in a family with multiple Hb disorders. American journal of hematology. vol 61. issue 3. 1999-07-26. PMID:10398311. |
sequencing of the beta-globin gene and alpha cluster mapping in the propositus and his brother showed a previously undescribed beta-globin variant:hb iraq-halabja, beta10(a7) ala-->val (gcc-->gtc), associated with beta0-thalassemia ivs-2 nt1 g-->a and either alpha-thal-2-3.7 kb deletion (brother), or alpha-globin gene triplication anti-3.7 kb type (propositus). |
1999-07-26 |
2023-08-12 |
Not clear |
| L Zhang, E F Plo. Amino acid sequences within the alpha subunit of integrin alpha M beta 2 (Mac-1) critical for specific recognition of C3bi. Biochemistry. vol 38. issue 25. 1999-07-22. PMID:10387051. |
mutation of k245 to ala significantly reduced c3bi binding but had no effect on binding of another alpha m beta 2 i domain ligand, nif. |
1999-07-22 |
2023-08-12 |
Not clear |
| M A Jimenez, J A Evangelio, C Aranda, A Lopez-Brauet, D Andreu, M Rico, R Lagos, J M Andreu, O Monasteri. Helicity of alpha(404-451) and beta(394-445) tubulin C-terminal recombinant peptides. Protein science : a publication of the Protein Society. vol 8. issue 4. 1999-07-14. PMID:10211825. |
the apolar faces of the helix in both alpha and beta chains share a characteristic sequence of conserved residues ala,met(+4),leu(+7),tyr(+11). |
1999-07-14 |
2023-08-12 |
Not clear |
| I Korn, S Gutkind, N Srinivasan, T L Blundell, C C Allende, J E Allend. Interactions of protein kinase CK2 subunits. Molecular and cellular biochemistry. vol 191. issue 1-2. 1999-04-29. PMID:10094395. |
the inactive mutant of ck2alpha that has asp 156 mutated to ala (ck2alphaa156) is able to bind the ck2beta subunit and to compete effectively in this binding with wild-type subunits alpha and alpha'. |
1999-04-29 |
2023-08-12 |
Not clear |
| J F Prost, D Nègre, C Oudot, K Murakami, A Ishihama, A J Cozzone, J C Corta. Cra-dependent transcriptional activation of the icd gene of Escherichia coli. Journal of bacteriology. vol 181. issue 3. 1999-02-23. PMID:9922253. |
we observed that (i) the cra factor binds to and activates transcription from a site centered at position -76.5 within the icd p2 promoter region and (ii) three particular mutations in the c-terminal end of the alpha subunit of rna polymerase (l262a, r265a, and n268a) considerably diminish transcription initiating from the icd p2 promoter, as shown by in vitro experiments performed in the presence of mutant rna polymerases carrying ala substitutions. |
1999-02-23 |
2023-08-12 |
Not clear |
| O M El-Agnaf, D J Guthrie, D M Walsh, G B Irvin. The influence of the central region containing residues 19-25 on the aggregation properties and secondary structure of Alzheimer's beta-amyloid peptide. European journal of biochemistry. vol 256. issue 3. 1998-11-05. PMID:9780232. |
the mutation from ala to gly at position 21 increased the rate of aggregation and altered the tendency to adopt secondary structure in the direction away from alpha helix and towards beta sheet. |
1998-11-05 |
2023-08-12 |
Not clear |
| M Natochin, N O Artemye. A single mutation Asp229 --> Ser confers upon Gs alpha the ability to interact with regulators of G protein signaling. Biochemistry. vol 37. issue 39. 1998-10-28. PMID:9753466. |
to test this hypothesis, we have investigated the possibility of generating an interaction between gs alpha and rgs proteins by substituting gs alpha asp229 with ser and replacing the potential gs alpha asp229 contact residues in rgs16, glu129 and asn131, by ala and ser, respectively. |
1998-10-28 |
2023-08-12 |
Not clear |
| H J Dyson, L Bolinger, V A Feher, J J Osterhout, J Yao, P E Wrigh. Sequence requirements for stabilization of a peptide reverse turn in water solution--proline is not essential for stability. European journal of biochemistry. vol 255. issue 2. 1998-09-09. PMID:9716389. |
within this framework, the identity of the first residue of the turn (x in xpgdv) does not greatly influence the turn population, although a small but significant increase is observed for residues such as ala which have a preference for backbone conformations in the alpha region of (phi,psi) space. |
1998-09-09 |
2023-08-12 |
Not clear |
| M Hanner, R Vianna-Jorge, A Kamassah, W A Schmalhofer, H G Knaus, G J Kaczorowski, M L Garci. The beta subunit of the high conductance calcium-activated potassium channel. Identification of residues involved in charybdotoxin binding. The Journal of biological chemistry. vol 273. issue 26. 1998-08-03. PMID:9632689. |
to identify those residues in the beta subunit that are responsible for this change in binding affinity, ala scanning mutagenesis was carried out along the extracellular loop of beta, and the resulting effects on 125i-chtx binding were determined after coexpression with the alpha subunit. |
1998-08-03 |
2023-08-12 |
Not clear |
| Y J Huang, D Walker, W Chen, M Klingbeil, R Komunieck. Expression of pyruvate dehydrogenase isoforms during the aerobic/anaerobic transition in the development of the parasitic nematode Ascaris suum: altered stoichiometry of phosphorylation/inactivation. Archives of biochemistry and biophysics. vol 352. issue 2. 1998-06-03. PMID:9587415. |
to examine this altered pattern of phosphorylation, the two phosphorylation sites in each e1 alpha subunit of the r alpha i (site 1 and site 2) were changed either individually or together from ser to ala by site-directed mutagenesis. |
1998-06-03 |
2023-08-12 |
human |
| H Cho, B V Plap. Specificity of alcohol dehydrogenases for sulfoxides. Biochemistry. vol 37. issue 13. 1998-04-23. PMID:9521768. |
eqadh binds the s isomers of 3-butylthiolane 1-oxides, hexyl methyl sulfoxide, and phenyl methyl sulfoxide more tightly than the r isomers, but eqadh with phe-93 substituted with ala and the human alpha enzyme (with ala-93) prefer (r)-phenyl methyl sulfoxide, apparently because the phenyl ring fits into the space near residue 93. |
1998-04-23 |
2023-08-12 |
human |
| O N Ozoline, K Murakami, T Negishi, N Fujita, A Ishiham. Specific fluorescent labeling of two functional domains in RNA polymerase alpha subunit. Proteins. vol 30. issue 2. 1998-04-10. PMID:9489926. |
substitution of ala for both cys-269 and cys-176 completely eliminates the reactivity of alpha subunit against the fluorescent dye, supporting the prediction that another reactive amino acid under native conformation is cys-176, which is positioned within or near the region important for alpha dimerization and its binding of beta' subunit. |
1998-04-10 |
2023-08-12 |
Not clear |
| D Nègre, C Oudot, J F Prost, K Murakami, A Ishihama, A J Cozzone, J C Corta. FruR-mediated transcriptional activation at the ppsA promoter of Escherichia coli. Journal of molecular biology. vol 276. issue 2. 1998-04-07. PMID:9512708. |
direct contacts between the upstream activating dna and rna polymerase were studied in an in vitro transcription assay by using reconstituted rna polymerase mutants containing ala substitutions in c-terminal domain of their alpha subunit. |
1998-04-07 |
2023-08-12 |
Not clear |
| M A Broda, B Rzeszotarska, L Smełka, M Rospen. Conformational investigation of alpha,beta-dehydropeptides. VIII. N-acetyl-alpha,beta-dehydroamino acid N'-methylamides: conformation and electron density perturbation from infrared and theoretical studies. The journal of peptide research : official journal of the American Peptide Society. vol 50. issue 5. 1998-02-26. PMID:9401918. |
the fourier transform infrared spectra are analyzed in the regions of vs(n-h), amide i, amide ii and vs(c alpha = c beta) bands for a series of ac-delta xaa-nhme, where delta xaa = delta ala, (z)-delta abu, (z)-delta leu, (z)-delta phe and delta val, to determine the predominant solution conformation of these alpha,beta-dehydropeptide-related molecules and the electron distribution perturbation in their amide bonds. |
1998-02-26 |
2023-08-12 |
Not clear |
| M A Kapphahn, J B Shab. Contribution of the carboxyl-terminal regional of the cAMP-dependent protein kinase type I alpha regulatory subunit to cyclic nucleotide interactions. Archives of biochemistry and biophysics. vol 348. issue 2. 1998-02-09. PMID:9434747. |
the parent ri alpha subunit contained an ala to thr mutation at position 334 so that it would bind both camp and cgmp with high affinity. |
1998-02-09 |
2023-08-12 |
Not clear |
| E Benedetti, E Gavuzzo, A Santini, D R Kent, Y F Zhu, Q Zhu, C Mahr, M Goodma. Sweet and bitter taste: structure and conformations of two aspartame dipeptide analogues. Journal of peptide science : an official publication of the European Peptide Society. vol 1. issue 6. 1997-09-23. PMID:9223014. |
in the second residue, a left-handed helical conformation is observed for the (alpha me)phe residue of l-asp-d-(alpha me)phe-ome with phi 2 = 49.0 degrees and psi 2 = 47.9 degrees, while the ala residue of l-asp-d-ala-otmcp adopts a semi-extended conformation characterized by dihedral angles phi 2 = 62.8 degrees and psi 2 = -139.9 degrees. |
1997-09-23 |
2023-08-12 |
Not clear |
| T Oh-ham. Evolutionary consideration on 5-aminolevulinate synthase in nature. Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life. vol 27. issue 4. 1997-09-22. PMID:9249985. |
from the phylogenetic distribution it is shown that distribution of ala synthase is restricted to the alpha subclass of purple bacteria in prokaryotes, and further distributed to mitochondria of eukaryotes. |
1997-09-22 |
2023-08-12 |
Not clear |
| T Oh-ham. Evolutionary consideration on 5-aminolevulinate synthase in nature. Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life. vol 27. issue 4. 1997-09-22. PMID:9249985. |
evolution of ala synthase in the alpha subclass of purple bacteria is discussed in relation to the energy-generating and biosynthetic devices in subclasses of this bacteria. |
1997-09-22 |
2023-08-12 |
Not clear |