| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Zhixin Liu, Youjie Li, Gaohan She, Xiaofeng Zheng, Liying Shao, Pingyu Wang, Min Pang, Shuyang Xie, Yunxiao Su. Resveratrol induces cervical cancer HeLa cell apoptosis through the activation and nuclear translocation promotion of FOXO3a. Die Pharmazie. vol 75. issue 6. 2021-05-20. PMID:32539920. |
our data showed that resveratrol could significantly inhibit hela cell proliferation and induce their apoptosis, as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) colorimetric assay and flow cytometry. |
2021-05-20 |
2023-08-13 |
Not clear |
| Zhen Zhang, Xin Chen, Sichen Li. Role of Sirtuin-1 in Neonatal Hypoxic-Ischemic Encephalopathy and Its Underlying Mechanism. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-20. PMID:32826847. |
cell apoptosis and cell viability were analyzed by flow cytometry and mtt assay. |
2021-05-20 |
2023-08-13 |
rat |
| Jin Zhao, Liping Su, Jianjun Jian. Long Non-Coding RNA Paternally Expressed Imprinted Gene 10 (PEG10) Elevates Diffuse Large B-Cell Lymphoma Progression by Regulating Kinesin Family Member 2A (KIF2A) via Targeting MiR-101-3p. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-20. PMID:32976381. |
the biological behaviors including cell proliferation, apoptosis, migration, and invasion were determined by mtt assay, flow cytometry analysis, and transwell assays, respectively. |
2021-05-20 |
2023-08-13 |
Not clear |
| Shaohua Mi, Pengfei Wang, Lejun Li. miR-188-3p Inhibits Vascular Smooth Muscle Cell Proliferation and Migration by Targeting Fibroblast Growth Factor 1 (FGF1). Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-20. PMID:33020467. |
the proliferation, migration, and apoptosis of the cells were determined using mtt, brdu, and transwell assays, as well as flow cytometry analysis. |
2021-05-20 |
2023-08-13 |
mouse |
| Amber M Tavener, Megan C Phelps, Richard L Daniel. Anthracycline-induced cytotoxicity in the GL261 glioma model system. Molecular biology reports. vol 48. issue 1. 2021-05-20. PMID:33387196. |
mtt assays quantified anthracycline effects on cell viability, comet assays examined doxorubicin genotoxicity, and flow cytometry with annexin v/pi staining characterized doxorubicin-induced apoptosis and necrosis. |
2021-05-20 |
2023-08-13 |
Not clear |
| Weifu Zhou, Hui Li, Shuo Shang, Feng Li. lncRNA KCNQ1OT1 reverses the effect of sevoflurane on hepatocellular carcinoma progression via regulating the miR-29a-3p/CBX3 axis. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 54. issue 7. 2021-05-20. PMID:34008749. |
cell proliferation, apoptosis, migration, and invasion were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometry, and transwell assays, respectively. |
2021-05-20 |
2023-08-13 |
Not clear |
| Fatemeh Vahidian, Elham Safarzadeh, Ali Mohammadi, Shiva Najjary, Behzad Mansoori, Jafar Majidi, Zohreh Babaloo, Ayoub Aghanejad, Mahdi Abdoli Shadbad, Ahad Mokhtarzadeh, Behzad Baradara. siRNA-mediated silencing of CD44 delivered by Jet Pei enhanced Doxorubicin chemo sensitivity and altered miRNA expression in human breast cancer cell line (MDA-MB468). Molecular biology reports. vol 47. issue 12. 2021-05-19. PMID:33206362. |
the mtt assay, wound healing test, colony formation assay, dapi staining, and flow cytometry were performed to investigate the tumoral cell viability, migration, clonogenesis, and apoptosis progression. |
2021-05-19 |
2023-08-13 |
human |
| Hongyan Ma, Baoyi Yang, Lu Yu, Yang Gao, Xiangmei Ye, Ying Liu, Zhengtian Li, Hulun Li, Enyou L. Sevoflurane protects the liver from ischemia-reperfusion injury by regulating Nrf2/HO-1 pathway. European journal of pharmacology. vol 898. 2021-05-19. PMID:33631180. |
inflammatory factor levels, cell viability, apoptosis were detected by enzyme-linked immunosorbent assay (elisa), mtt assay, and flow cytometry, respectively.in the i/r group, liver damage was severe, apoptosis-positive cells were increased, ho-1 and nuclear nrf2 expressions were increased, and cytosolic nrf2 expression was decreased. |
2021-05-19 |
2023-08-13 |
rat |
| Qianru Yin, Jiayi Gu, Yingxue Qi, Yuxin Lu, Lanxin Yang, Jianwen Liu, Xin Lian. ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells. European journal of pharmacology. vol 898. 2021-05-19. PMID:33675784. |
by mtt analysis, wound healing assay, and flow cytometry, we found that overexpression/knockdown of adam28 expression in gastric cancer cells can regulate cell proliferation, apoptosis and migration in vitro. |
2021-05-19 |
2023-08-13 |
human |
| Lei Jiao, Yingchun Shao, Qi Yu, Mengmeng Li, Yanying Wang, Manyu Gong, Xuewen Yang, Tianyi Liu, Zhange Li, Heng Liu, Yuanyuan Zhang, Zhongyue Tan, Lihua Sun, Lina Xuan, Hongli Yin, Yong Zhang, Benzhi Cai, Ying Zhang, Baofeng Yan. GDF11 replenishment protects against hypoxia-mediated apoptosis in cardiomyocytes by regulating autophagy. European journal of pharmacology. vol 885. 2021-05-18. PMID:32861661. |
by using the mtt assay, flow cytometry assay, live/dead® viability/cytotoxicity kit stains and tunel assay, we found that exogenous gdf11 decreased apoptosis caused by prolonged hypoxia in cardiomyocytes. |
2021-05-18 |
2023-08-13 |
mouse |
| Jie Yang, Wen-Wen Mu, Guo-Yun Li. Synthesis and evaluation of the anticancer activity of bischalcone analogs in human lung carcinoma (A549) cell line. European journal of pharmacology. vol 888. 2021-05-17. PMID:32798508. |
in this study, we synthesized a series of bischalcone analogs via aldol condensation reaction; mtt method was used to evaluate the antiproliferation effects; the 2',7'-dichlorofluorescein fluorescence assay was used to determine the intracellular reactive oxygen species levels; the glutathione reductase-dtnb recycling assay was used to detect the redox imbalance; determination of thiobarbituric acid-reactive substance was used to evaluate the lipid peroxidation; rhodamine 123 was used to test the mitochondrial membrane potential (mmp); the fitc/pi kit was used to detect the apoptosis; western blotting was used to detect the expression of bax and caspase 3. |
2021-05-17 |
2023-08-13 |
human |
| Tayebeh Rezaei, Maryam Hejazi, Behzad Mansoori, Ali Mohammadi, Mohammad Amini, Jafar Mosafer, Sarah Rezaei, Ahad Mokhtarzadeh, Behzad Baradara. microRNA-181a mediates the chemo-sensitivity of glioblastoma to carmustine and regulates cell proliferation, migration, and apoptosis. European journal of pharmacology. vol 888. 2021-05-17. PMID:32810491. |
mtt assay was used to determine cell viability and annexin v/pi and dapi staining were employed to evaluate apoptosis induction. |
2021-05-17 |
2023-08-13 |
human |
| Tao Zhang, Fen Qi. Icariin Protects Mouse Insulinoma Min6 Cell Function by Activating the PI3K/AKT Pathway. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-17. PMID:32885795. |
min6 cells were treated with ica for 2 h, followed by 5 mg/dl ua for 24 h, and cell viability, apoptosis, apoptosis-associated protein levels and insulin secretion were assessed by mtt, flow cytometry, western blotting and glucose-stimulated insulin secretion assays, respectively. |
2021-05-17 |
2023-08-13 |
mouse |
| Mingxiang Cao, Di Xiao, Xubei Din. The anti-tumor effect of ursolic acid on papillary thyroid carcinoma via suppressing Fibronectin-1. Bioscience, biotechnology, and biochemistry. vol 84. issue 12. 2021-05-17. PMID:32942951. |
human ptc cells were under ua treatment, and cell viability, clone formation, and apoptosis were measured by mtt assay, clone formation assay, and flow cytometry, respectively. |
2021-05-17 |
2023-08-13 |
human |
| Zeinab Mohseni, Hamid Sedighian, Raheleh Halabian, Jafar Amani, Elham Behzadi, Abbas Ali Imani Foolad. Potent in vitro antitumor activity of B-subunit of Shiga toxin conjugated to the diphtheria toxin against breast cancer. European journal of pharmacology. vol 899. 2021-05-17. PMID:33753109. |
t47d, skbr3, 4t1 and mcf7 cell lines were treated separately with purified dt389-stxb recombinant protein and functional activity of dt389-stxb was analyzed by the cell enzyme-linked immunosorbentassay (elisa), mtt, icc, western blot and apoptosis tests. |
2021-05-17 |
2023-08-13 |
human |
| Y H Li, M F Lv, M S Lu, J P B. Bone marrow mesenchymal stem cell-derived exosomal MiR-338-3p represses progression of hepatocellular carcinoma by targeting ETS1. Journal of biological regulators and homeostatic agents. vol 35. issue 2. 2021-05-17. PMID:33884828. |
next, transwell assay and methyl thiazolyl tetrazolium (mtt) experiments were carried out to detect the effects of mir-338-3p and e26 transformation specific-1 (ets1) on the viability, invasion, migration, and apoptosis of hcc cells through the exosomes derived from bmscs. |
2021-05-17 |
2023-08-13 |
human |
| Shuyan Liu, Yun Zhang, Chang Huang, Shengyun Li. miR-215-5p is an anticancer gene in multiple myeloma by targeting RUNX1 and deactivating the PI3K/AKT/mTOR pathway. Journal of cellular biochemistry. vol 121. issue 2. 2021-05-14. PMID:31498483. |
cell viability was detected using the mtt method, while apoptosis and cell cycle were measured using flow cytometry. |
2021-05-14 |
2023-08-13 |
Not clear |
| Dong-Wei Yao, Qun Song, Xiao-Zhou H. Kinesin family member 23 (KIF23) contributes to the progression of bladder cancer cells in vitro and in vivo. Neoplasma. vol 68. issue 2. 2021-05-14. PMID:33231086. |
colony formation, mtt, and flow cytometry (fcm) assays were performed to detect its effects on bladder cancer cell proliferation and apoptosis, respectively. |
2021-05-14 |
2023-08-13 |
mouse |
| Lana S Rosa, Mariana L Santos, Joel P Abreu, Celso F Balthazar, Ramon S Rocha, Hugo L A Silva, Erick A Esmerino, Maria Carmela K H Duarte, Tatiana C Pimentel, Mônica Q Freitas, Márcia C Silva, Adriano G Cruz, Anderson J Teodor. Antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cell lines. Food research international (Ottawa, Ont.). vol 137. 2021-05-14. PMID:33233128. |
cell viability was determined by mtt assay and cell cycle arrest, and apoptosis by flow cytometry. |
2021-05-14 |
2023-08-13 |
human |
| Zahra Ranjbar-Navazi, Marziyeh Fathi, Elaheh Dalir Abdolahinia, Yadollah Omidi, Soodabeh Davara. MUC-1 aptamer conjugated InP/ZnS quantum dots/nanohydrogel fluorescent composite for mitochondria-mediated apoptosis in MCF-7 cells. Materials science & engineering. C, Materials for biological applications. vol 118. 2021-05-14. PMID:33255050. |
results obtained from in vitro analysis (mtt assay, apoptosis/necrosis assessment, evaluation of mitochondria targeting, and gene expression profiling) revealed that ap-nhg-qds-ptx-so ns could significantly target and inhibit mcf-7 cells and also induce mitochondria-mediated apoptosis. |
2021-05-14 |
2023-08-13 |
Not clear |