| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Qianru Yin, Jiayi Gu, Yingxue Qi, Yuxin Lu, Lanxin Yang, Jianwen Liu, Xin Lian. ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells. European journal of pharmacology. vol 898. 2021-05-19. PMID:33675784. |
by mtt analysis, wound healing assay, and flow cytometry, we found that overexpression/knockdown of adam28 expression in gastric cancer cells can regulate cell proliferation, apoptosis and migration in vitro. |
2021-05-19 |
2023-08-13 |
human |
| Lei Jiao, Yingchun Shao, Qi Yu, Mengmeng Li, Yanying Wang, Manyu Gong, Xuewen Yang, Tianyi Liu, Zhange Li, Heng Liu, Yuanyuan Zhang, Zhongyue Tan, Lihua Sun, Lina Xuan, Hongli Yin, Yong Zhang, Benzhi Cai, Ying Zhang, Baofeng Yan. GDF11 replenishment protects against hypoxia-mediated apoptosis in cardiomyocytes by regulating autophagy. European journal of pharmacology. vol 885. 2021-05-18. PMID:32861661. |
by using the mtt assay, flow cytometry assay, live/dead® viability/cytotoxicity kit stains and tunel assay, we found that exogenous gdf11 decreased apoptosis caused by prolonged hypoxia in cardiomyocytes. |
2021-05-18 |
2023-08-13 |
mouse |
| Jie Yang, Wen-Wen Mu, Guo-Yun Li. Synthesis and evaluation of the anticancer activity of bischalcone analogs in human lung carcinoma (A549) cell line. European journal of pharmacology. vol 888. 2021-05-17. PMID:32798508. |
in this study, we synthesized a series of bischalcone analogs via aldol condensation reaction; mtt method was used to evaluate the antiproliferation effects; the 2',7'-dichlorofluorescein fluorescence assay was used to determine the intracellular reactive oxygen species levels; the glutathione reductase-dtnb recycling assay was used to detect the redox imbalance; determination of thiobarbituric acid-reactive substance was used to evaluate the lipid peroxidation; rhodamine 123 was used to test the mitochondrial membrane potential (mmp); the fitc/pi kit was used to detect the apoptosis; western blotting was used to detect the expression of bax and caspase 3. |
2021-05-17 |
2023-08-13 |
human |
| Tayebeh Rezaei, Maryam Hejazi, Behzad Mansoori, Ali Mohammadi, Mohammad Amini, Jafar Mosafer, Sarah Rezaei, Ahad Mokhtarzadeh, Behzad Baradara. microRNA-181a mediates the chemo-sensitivity of glioblastoma to carmustine and regulates cell proliferation, migration, and apoptosis. European journal of pharmacology. vol 888. 2021-05-17. PMID:32810491. |
mtt assay was used to determine cell viability and annexin v/pi and dapi staining were employed to evaluate apoptosis induction. |
2021-05-17 |
2023-08-13 |
human |
| Tao Zhang, Fen Qi. Icariin Protects Mouse Insulinoma Min6 Cell Function by Activating the PI3K/AKT Pathway. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-17. PMID:32885795. |
min6 cells were treated with ica for 2 h, followed by 5 mg/dl ua for 24 h, and cell viability, apoptosis, apoptosis-associated protein levels and insulin secretion were assessed by mtt, flow cytometry, western blotting and glucose-stimulated insulin secretion assays, respectively. |
2021-05-17 |
2023-08-13 |
mouse |
| Mingxiang Cao, Di Xiao, Xubei Din. The anti-tumor effect of ursolic acid on papillary thyroid carcinoma via suppressing Fibronectin-1. Bioscience, biotechnology, and biochemistry. vol 84. issue 12. 2021-05-17. PMID:32942951. |
human ptc cells were under ua treatment, and cell viability, clone formation, and apoptosis were measured by mtt assay, clone formation assay, and flow cytometry, respectively. |
2021-05-17 |
2023-08-13 |
human |
| Zeinab Mohseni, Hamid Sedighian, Raheleh Halabian, Jafar Amani, Elham Behzadi, Abbas Ali Imani Foolad. Potent in vitro antitumor activity of B-subunit of Shiga toxin conjugated to the diphtheria toxin against breast cancer. European journal of pharmacology. vol 899. 2021-05-17. PMID:33753109. |
t47d, skbr3, 4t1 and mcf7 cell lines were treated separately with purified dt389-stxb recombinant protein and functional activity of dt389-stxb was analyzed by the cell enzyme-linked immunosorbentassay (elisa), mtt, icc, western blot and apoptosis tests. |
2021-05-17 |
2023-08-13 |
human |
| Y H Li, M F Lv, M S Lu, J P B. Bone marrow mesenchymal stem cell-derived exosomal MiR-338-3p represses progression of hepatocellular carcinoma by targeting ETS1. Journal of biological regulators and homeostatic agents. vol 35. issue 2. 2021-05-17. PMID:33884828. |
next, transwell assay and methyl thiazolyl tetrazolium (mtt) experiments were carried out to detect the effects of mir-338-3p and e26 transformation specific-1 (ets1) on the viability, invasion, migration, and apoptosis of hcc cells through the exosomes derived from bmscs. |
2021-05-17 |
2023-08-13 |
human |
| Shuyan Liu, Yun Zhang, Chang Huang, Shengyun Li. miR-215-5p is an anticancer gene in multiple myeloma by targeting RUNX1 and deactivating the PI3K/AKT/mTOR pathway. Journal of cellular biochemistry. vol 121. issue 2. 2021-05-14. PMID:31498483. |
cell viability was detected using the mtt method, while apoptosis and cell cycle were measured using flow cytometry. |
2021-05-14 |
2023-08-13 |
Not clear |
| Dong-Wei Yao, Qun Song, Xiao-Zhou H. Kinesin family member 23 (KIF23) contributes to the progression of bladder cancer cells in vitro and in vivo. Neoplasma. vol 68. issue 2. 2021-05-14. PMID:33231086. |
colony formation, mtt, and flow cytometry (fcm) assays were performed to detect its effects on bladder cancer cell proliferation and apoptosis, respectively. |
2021-05-14 |
2023-08-13 |
mouse |
| Lana S Rosa, Mariana L Santos, Joel P Abreu, Celso F Balthazar, Ramon S Rocha, Hugo L A Silva, Erick A Esmerino, Maria Carmela K H Duarte, Tatiana C Pimentel, Mônica Q Freitas, Márcia C Silva, Adriano G Cruz, Anderson J Teodor. Antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cell lines. Food research international (Ottawa, Ont.). vol 137. 2021-05-14. PMID:33233128. |
cell viability was determined by mtt assay and cell cycle arrest, and apoptosis by flow cytometry. |
2021-05-14 |
2023-08-13 |
human |
| Zahra Ranjbar-Navazi, Marziyeh Fathi, Elaheh Dalir Abdolahinia, Yadollah Omidi, Soodabeh Davara. MUC-1 aptamer conjugated InP/ZnS quantum dots/nanohydrogel fluorescent composite for mitochondria-mediated apoptosis in MCF-7 cells. Materials science & engineering. C, Materials for biological applications. vol 118. 2021-05-14. PMID:33255050. |
results obtained from in vitro analysis (mtt assay, apoptosis/necrosis assessment, evaluation of mitochondria targeting, and gene expression profiling) revealed that ap-nhg-qds-ptx-so ns could significantly target and inhibit mcf-7 cells and also induce mitochondria-mediated apoptosis. |
2021-05-14 |
2023-08-13 |
Not clear |
| Asrin Pakravan, Roya Salehi, Mehrdad Mahka. Comparison study on the effect of gold nanoparticles shape in the forms of star, hallow, cage, rods, and Si-Au and Fe-Au core-shell on photothermal cancer treatment. Photodiagnosis and photodynamic therapy. vol 33. 2021-05-14. PMID:33307234. |
the ptt potential of these gnps was compared on mcf7 cells in vitro using mtt assay, cell cycle, and annexin-v apoptosis assay. |
2021-05-14 |
2023-08-13 |
Not clear |
| Jing Xu, Chunyang Wang, Fanjie Meng, Pengjuan X. Long non‑coding RNA H19 inhibition ameliorates oxygen‑glucose deprivation‑induced cell apoptosis and inflammatory cytokine expression by regulating the microRNA‑29b/SIRT1/PGC‑1α axis. Molecular medicine reports. vol 23. issue 2. 2021-05-14. PMID:33313940. |
following ogd treatment, mtt, flow cytometry, elisa, rt‑qpcr and western blotting assays were performed to assess cell proliferation, cell apoptosis, inflammatory cytokine concentrations, and lncrna h19, mir‑29b, sirt1, pgc‑1α expression levels, respectively. |
2021-05-14 |
2023-08-13 |
mouse |
| Cheng Gao, Xiequn Zou, Huan Chen, Rong Shang, Bing Wan. Long Non-Coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1)Relieves Sepsis-Induced Kidney Injury and Lipopolysaccharide (LPS)-Induced Inflammation in HK-2 Cells. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-13. PMID:32724027. |
after treatment with lipopolysaccharide (lps), the biological behaviors of human renal tubular epithelial cells (hk-2), such as proliferation and apoptosis, were determined using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2h-tetrazol-3-ium bromide (mtt) and flow cytometry assays, respectively. |
2021-05-13 |
2023-08-13 |
human |
| Hanke Geng, Kaixuan Li, Qi Pan, Shaohui Tao, Chunjuan Li, Haiwei Zhao, Xiaojun Zha. Identification and Expression of Several Circular RNAs and Knockdown of hsa_circ_0005556 Exerts Oncogenic Functions by miR-767-5p in Gastric Cancer. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-13. PMID:32728015. |
the effects of circ_0005556 and mir-767-5p on gc cell viability, apoptosis, migration, and invasion were assessed by mtt, flow cytometry, wound-healing and in vitro transwell experiments, respectively. |
2021-05-13 |
2023-08-13 |
Not clear |
| Xi Jiang, Yulin Huan. Curcumin Derivative C086 Combined with Cisplatin Inhibits Proliferation of Osteosarcoma Cells. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-13. PMID:32734935. |
functions of cur c086+cisplatin on proliferation ability, apoptosis response, and metastatic potential of mg-63 cells were determined by mtt, flow cytometry, hoechst 33258 staining and transwell assays, respectively. |
2021-05-13 |
2023-08-13 |
Not clear |
| Ziqiang Cui, Yaping Lin, Yuhong Liu, Ling Cao, Li Cu. Retinoic Acid-Platinum (II) Complex [RT-Pt(II)] Protects Against Rheumatoid Arthritis in Mice via MEK/Nuclear Factor kappa B (NF-κB) Pathway Downregulation. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-13. PMID:32741960. |
material and methods mh7a cell viability was determined by mtt assay and apoptosis was assessed using facscalibur flow cytometry. |
2021-05-13 |
2023-08-13 |
mouse |
| Xindong He, Yongqiang Don. Ankylosis progressive homolog upregulation inhibits cell viability and mineralization during fibroblast ossification by regulating the Wnt/β‑catenin signaling pathway. Molecular medicine reports. vol 22. issue 6. 2021-05-13. PMID:33173993. |
cell viability, apoptosis and mineralization were analyzed using mtt assays, flow cytometry and alizarin red staining, respectively. |
2021-05-13 |
2023-08-13 |
human |
| Yong Cui, Qi Wang, Jing Lin, Lei Zhang, Chi Zhang, Huairui Chen, Jun Qian, Chun Lu. miRNA-193a-3p Regulates the AKT2 Pathway to Inhibit the Growth and Promote the Apoptosis of Glioma Cells by Targeting ALKBH5. Frontiers in oncology. vol 11. 2021-05-13. PMID:33968717. |
the effects of mir-193a-3p on cell growth and apoptosis were investigated using mtt, colony-forming, and flow cytometry assays. |
2021-05-13 |
2023-08-13 |
mouse |